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TransIT®-mRNA Transfection Kit

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제조사 제품코드 제품명 용량 가격
(부가세별도)
비고 사용자매뉴얼
Mirus
MIR 2225
TransIT-mRNA Transfection Kit
관련학술 관심상품등록 구매하기
0.4 ml
가격문의   ml036_transit_mrna_transfection_kit.pdf
Mirus
MIR 2250
TransIT-mRNA Transfection Kit
관련학술 관심상품등록 구매하기
1.0 ml
가격문의  
Mirus
MIR 2255
TransIT-mRNA Transfection Kit
관련학술 관심상품등록 구매하기
1.0 ml×5
가격문의  
Mirus
MIR 2256
TransIT-mRNA Transfection Kit
관련학술 관심상품등록 구매하기
1.0 ml×10
가격문의  

Each Kit is supplied with the TransIT-mRNA Transfection Reagent and the mRNA Boost Reagent.
  • Low Cellular Toxicity - Maintain cell density and reduce experimental biases.
  • High Efficiency Delivery - Achieve RNA delivery in a large population of cells to ensure experimental success.
  • Serum Compatible - Perform transfections in the presence of serum which eliminates the need for a media change and maintains cellular health.
  • Deliver Various Sizes of RNA - Ideal for specialized applications, such as viral production and protein expression from mRNA.
Recent Advances in Stem Cell Technology Utilize mRNA Transfection to Generate Induced Pluripotent Stem Cells. Find out more in the following articles.

Warren L, Manos PD, Ahfeldt T, Loh YH, Li H, Lau F, Ebina W, Mandal PK, Smith ZD, Meissner A, Daley GQ, Brack AS, Collins JJ, Cowan C, Schlaeger TM, Rossi DJ. 2010. Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA. Cell Stem Cell. Volume 7, Issue 5, 618-630.

Angel M, Yanik MF. 2010. Innate Immune Suppression Enables Frequent Transfection with RNA Encoding Reprogramming Proteins. PLoS One. Jul 23;5(7):e11756. Pubmed

Cell lines successfully transfected at Mirus usingTransIT-mRNA Transfection Kit



Figure 1. The TransIT-mRNA Transfection Kit Efficiently Delivers LacZ mRNA to CHO-K1 Cells. Using the TransIT-mRNA Transfection Kit, CHO-K1 cells were mock transfected (A) or transfected with a capped and polyadenylated LacZ encoding mRNA (B). Approximately 18 hrs post-transfection the cells were stained using Mirus Bio’s Beta-gal Staining Kit to identify the LacZ transfected cells.



Figure 2. The TransIT-mRNA Transfection Kit Efficiently Delivers mRNA to a Variety of Cell Lines. Using the TransIT-mRNA Transfection Kit, cells were transfected with a capped and polyadenylated EGFP encoding mRNA. Approximately 18 hrs post-transfection the cells were analyzed by flow cytometry to identify the EGFP expressing cells.



Figure 3. High Level Luciferase Expression after Delivery of a Luciferase mRNA using the TransIT-mRNA Transfection Kit. Cells in 12-well plates were transfected with a capped and polyadenylated mRNA encoding luciferase using the TransIT-mRNA Transfection Kit. Approximately 18 hrs post-transfection the cells were harvested and the total luciferase activity per well was determined.



Figure 4. Achieve Superior mRNA Delivery Performance with the TransIT-mRNA Transfection Kit. The indicated cell lines were transfected at approximately 80% confluency with a capped and polyadenylated mRNA encoding firefly luciferase using either the TransIT-mRNA Transfection Kit or Competitor Reagent Q. Approximately 18 hours post-transfection, the cells were harvested, lysed and the amount of luciferase activity present in each well was determined. The results were normalized to the activity of the TransIT-mRNA Kit transfected samples (set as 100%). The black diamonds illustrate the average percent cell confluency compared to the TransIT-mRNA transfected wells for each cell line demonstrating the toxicity of Reagent Q. In each experiment, the cells transfected with the TransIT-mRNA Transfection Kit were approximately 100% confluent at the time of harvest.

Keyword : V2225,MIR2225,V2250,MIR2250,V2255T,MIR2255,V2256,MIR2256
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