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범용적인 Transfection 시약(동물 유래 성분 free)

TransIT®-2020 Transfection Reagent

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제조사 제품코드 제품명 용량 가격
(부가세별도)
비고 사용자매뉴얼
Mirus
MIR 5404
TransIT-2020 Transfection Reagent
관련학술 관심상품등록 구매하기
0.4 ml
가격문의   가격할인
11.01 ~ 12.13
ml055_transit_2020.pdf
Mirus
MIR 5400
TransIT-2020 Transfection Reagent
관련학술 관심상품등록 구매하기
1 ml
가격문의   가격할인
11.01 ~ 12.13
Mirus
MIR 5405
TransIT-2020 Transfection Reagent
관련학술 관심상품등록 구매하기
1 ml×5
가격문의   가격할인
11.01 ~ 12.13
Mirus
MIR 5406
TransIT-2020 Transfection Reagent
관련학술 관심상품등록 구매하기
1 ml×10
가격문의   가격할인
11.01 ~ 12.13

  • 광범위한 세포에 DNA를 고효율로 도입 가능
  • 낮은 세포 독성으로 배지 교환 조작이 불필요
  • 동물 유래 성분 비포함 (Animal Origin Free)
제품설명
TransIT®-2020은 광범위한 mammalian cell에 뛰어난 효율로 DNA plasmid를 도입하는 제품이다. TransIT-2020®은 유전자 도입이 어려운 세포의 일시적인 (transient) 유전자 도입과 영구적인 (stable) 유전자 도입에 적합하다. TransIT-2020®은 동물 유래 성분이 포함하지 않으며, serum이 포함된 배지에 사용 가능하기 때문에, transfection 이후 번거로운 배지 교체 작업이 필요 없이 간편하게 사용 할 수 있다.


Figure 1. TransIT®-2020 Reagent Exhibits Higher Expression and Lower Cellular Toxicity Compared to Other Transfection Reagents. Human umbilical vein endothelial cells (HUVEC) were transfected with a luciferase expression plasmid using the designated reagents at the reagent-to-DNA ratios indicated beneath each bar. Transfections were performed in 96-well plates using 0.1 μg of plasmid DNA per well. Luciferase expression (bar graph) and lactate dehydrogenase (LDH) levels (line graph) were measured at 24 hours post-transfection. LDH levels are reported as % cytotoxicity compared to cells alone and were measured using a commercially available colorimetric assay; all values at or below zero are represented as zero on graph. Error bars represent the standard deviation of triplicate wells.


Figure 2. High Performance Plasmid Transfection. Primary Human Small Epithelial Cells (HSAEpic) were transfected using  TransIT®-2020 and an EGFP expression plasmid (4:1 reagent-to-DNA ratio). Images were taken 24 hours post-transfection using a Zeiss axiovert inverted fluorescence microscope.


Figure 3. Superior Gene Expression in a Broad Spectrum of Cell Types. The indicated cell types were transfected in 96-well plates with a luciferase expression plasmid (0.1 μg/well) according to industry accepted testing protocols. Reagent to DNA ratios were optimized for each cell type:  TransIT®-2020 (Mirus Bio, 2:1 or 3:1), FuGENE® HD (Roche, 3.5:1), Lipofectamine® 2000 (Life Technologies, 1.5:1, 3:1 or 5:1).  Luciferase activity was measured 24 hours post-transfection. Values were normalized to TransIT®-2020 and presented as a percentage of luciferase expression. FuGENE® is a registered trademark of Fugent LLC. Lipofectamine® is a trademark of Life Technologies Corporation.
보존 -20℃






Keyword : V5404,MIR5404,insect,virus,V5400,MIR5400,V5405,MIR5405,V5406,MIR5406,
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