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TransIT-PRO¢ç Transfection Reagent & Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Mirus
MIR 5700
TransIT-PRO¢ç Transfection Kit (includes PRO Boost Reagent)
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 ml
°¡°Ý¹®ÀÇ   transit_pro_transfection_kit.pdf
Mirus
MIR 5760
TransIT-PRO¢ç Transfection Kit (includes PRO Boost Reagent)
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ml
°¡°Ý¹®ÀÇ  
Mirus
MIR 5740
TransIT-PRO¢ç Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
1 ml
°¡°Ý¹®ÀÇ   transit-pro-transfection-reagent-protocol.pdf
Mirus
MIR 5750
TransIT-PRO¢ç Transfection Reagent
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ml
°¡°Ý¹®ÀÇ  



Á¦Ç°¼³¸í
TransIT-PRO Transfection Kit (includes Pro Boost reagent) Àº ºÎÀ¯ ¼¼Æ÷ÀÎ 293 cell°ú CHO cell¿¡¼­ ´Ü¹éÁúÀ» »ý»êÇϵµ·Ï °³¹ßµÈ DNA transfection ½Ã¾à°ú boost ½Ã¾àÀ¸·Î ±¸¼ºµÇ¾î
ÀÖ´Ù. PRO Boost Reagent´Â ¹èÁö¿¡ µû¶ó ¼±ÅÃÀûÀ¸·Î »ç¿ëÇÏ´Â ½Ã¾àÀ¸·Î ¹ßÇöÀ» Áõ°¡½ÃÅ°´Â ¿ªÇÒÀ» ÇÑ´Ù. TransIT-PRO Transfection Reagent¿Í PRO Boost Reagent´Â animal-origin free components·Î ±¸¼ºµÇ¾î ÀÖÀ¸¸ç ´Ù¾çÇÑ ¹èÁö¿¡ Àû¿ë °¡´ÉÇÏ´Ù. TransIT-PRO Transfection KitÀº transfection ÈÄÀÇ ¹èÁö ±³È¯ÀÌ ÇÊ¿äÇÏÁö ¾Ê°í transient transfectionÀ̳ª stable transfection ¸ðµÎ¿¡ Àû¿ë °¡´ÉÇÏ´Ù.
  • Suspension CHO¿Í 293 cell ¿ë transfection reagent
  • ´Ù¾çÇÑ CHO ¹èÁö Á¶¼º¿¡¼­ ȣȯ¼º ¿ì¼ö
  • ´Ù¾çÇÑ culture volume (4-400 §¢)¿¡ Àû¿ë °¡´É
TransIT-PRO¢ç Transfection Kit (includes PRO Boost Reagent)

Code

TransIT-PRO¢ç Transfection Reagent ¿ë·®

PRO Boost Reagent
¿ë·®

MIR5700

1 ml

1.5 ml

MIR 5760

10 ml

15 ml


¼Â¾÷ÀÌ °£ÆíÇÑ ´ë·® ´Ü¹éÁú »ý»ê system
CHOgro¢ç Expression System ÀÚ¼¼È÷º¸±â
Transfection ÈÄ ½Ã°£°æ°ú¿¡ µû¸¥ ¹ßÇöÀ² ºñ±³

Figure 1. Achieve high antibody titers using the TransIT-PRO¢ç Transfection Kit. Human IgG1 was produced by transient transfection using TransIT-PRO and PRO Boost Reagent, 25kDa linear PEI or FreeStyle¢â Max transfection reagents according to the manufacturers or published protocol. Transfections were performed using 1§¶ plasmid DNA per milliliter of culture and 0.5 x 106 cells/§¢ at the time of transfection. FreeStyle¢â CHO-S cells were cultured in 20 §¢ of FreeStyle¢â CHO Expression medium in 125 §¢ shake flasks. Day 3, 5 and 7 supernatants were clarified and analyzed using a human IgG-Fc sandwich ELISA.
°í°´ÀÌ Á÷Á¢ °æÇèÇÑ TransIT-PRO¢ç ³ôÀº ¹ßÇö

Figure 2. Evaluation of EGFP-C3 protein expression level upon transient transfection with different reagents. EGFP-C3 protein expression per viable cell at 37 ¡ÆC & 32 ¡ÆC.
[ÀÎ¿ë ³í¹®. Evaluation of transfection methods for transient gene expression in Chinese hamster ovary cells. Sou S, et al. ABB, 2013 Dec. Vol. 4.]
°ü·Ã Á¦Ç°
Endotoxin Á¦°Å¸¦ ÅëÇÑ ´Ü¹éÁú ¹ßÇö Çâ»ó MiraCLEAN¢ç Endotoxin Removal Kit ÀÚ¼¼È÷º¸±â
Transfection È¿À² È®Àοë Label IT¢ç Plasmid Delivery Control, Cy¢ç 3 ÀÚ¼¼È÷º¸±â

Keyword : V5700,MIR5700,GMP,V5760,MIR5760

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