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Transfection ½Ã¾à
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µ¶¼º Á¦°Å - Transfection È¿À² Áõ°¡
> MiraCLEAN¢ç Endotoxin Removal Kit
DNA ½Ã·á·Î ºÎÅÍ endotoxin Á¦°Å
MiraCLEAN¢ç Endotoxin Removal Kit
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Mirus
MIR 5910
MiraCLEAN Endotoxin Removal Kit
for 10 §· DNA
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Mirus
MIR 5900
MiraCLEAN Endotoxin Removal Kit
for 100 §· DNA
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Product index
Overview
[FAQ] Mirus transfecti..
Cell lineº° ¼±Åð¡À̵å
PACKAGE DELIVERY: The ..
´Ù¾çÇÑ ¼¼Æ÷/¹ü¿ëÀûÀÎ µµÀÔ..
TransIT-X2¢ç Transfect..
TransIT¢ç-LT1 Transfec..
TransIT¢ç-2020 Transfe..
Xfect¢â Transfection R..
CalPhos¢â Mammalian Tr..
È¿À²Áõ´ë¸¦ À§ÇÑ Æ¯¼öµµÀÔ½Ã..
TransIT-VirusGEN¢ç Tra..
TransIT¢ç-Lenti Transf..
TransIT-PRO¢ç Transfec..
RevIT¢â AAV Enhancer
VirusGEN¢ç AAV Transfe..
VirusGEN¢ç LV Transfec..
¼¼Æ÷¿¡ µû¸¥ È¿À²ÀûÀÎ À¯Àü..
TransIT¢ç-293 Transfec..
TransIT¢ç-BrCa Transfe..
TransIT¢ç-CHO Transfec..
TransIT-HeLaMONSTER¢ç ..
TransIT¢ç-Jurkat Trans..
TransIT¢ç-Keratinocyte..
TransIT¢ç-Insect Trans..
Xfect mESC Transfectio..
Electroporation °ü·Ã
Ingenio¢ç solution & k..
Ingenio¢ç cuvette, ¼Ò..
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TransIT¢ç-mRNA Transfe..
TransIT¢ç-Oligo Transf..
Xfect¢â RNA Transfecti..
Xfect¢â Protein Transf..
in-vivo delivery ½Ã½ºÅÛ
TransIT Hydrodynamic D..
µ¶¼º Á¦°Å - Transfection ..
MiraCLEAN¢ç Endotoxin ..
siRNA Transfection ½Ã¾à
Label IT Delivery Cont..
Label IT siRNA Tracker..
TransIT-QR Hydrodynami..
TransIT-siQUEST Transf..
TransIT-TKO Transfecti..
TransIT-X2 Dynamic Del..
siRNA ¹ßÇö ¡¤ ºÐ¼®
pSINsi vector ½Ã¸®Áî
Synthetic siRNA Quanti..
small RNA Cloning
pBApo-CMV vector ½Ã¸®..
pBApo-EF1¥á Vector ½Ã..
small RNA Cloning Kit
small RNA Gel Extracti..
RNAi °ü·Ã Kit
14-30 ssRNA Ladder Mar..
siRNA Ladder Marker
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E.coli Áõ½Ä½Ã ¿À¿°µÈ plasmidÀÇ endotoxinÀ» Á¦°ÅÇÏ´Â kit·Î, 2°³ÀÇ ½Ã¾àÀ¸·Î ±¸¼ºµÇ¾î °£ÆíÇÏ°Ô Á¦°Å °¡´ÉÇÏ´Ù.
in vitro
,
in vivo
À¯ÀüÀÚµµÀÔÀ» ½Ç½ÃÇÒ ¶§, plasmid¿¡ È¥ÀÔµÈ endotoxinÀÌ µµÀÔ È¿À²¿¡ ¿µÇâÀ» ÁÖ´Â °ÍÀ¸·Î ¾Ë·ÁÁ®ÀÖÀ¸¸ç, º» Á¦Ç°Àº plasmidÀÇ endotoxinÀ» Á¦°ÅÇÏ¿© ¾ÈÀüÇÏ°í È¿À²ÀûÀ¸·Î À¯ÀüÀÚ µµÀÔÀÌ °¡´ÉÇϵµ·Ï ÇÏ¿´´Ù
MiraCLEAN
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Endotoxin Removal Kit ±¸¼º
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EndoGO Extraction Reagent
2.2 ml
MiraCLEAN
¢ç
Buffer
2 ml
Endotoxin Á¦°Å È¿À²
Figure 1. The MiraCLEAN¢ç Kit Effectively Removes Endotoxin from Crude Plasmid DNA Preparations. Plasmid DNA was spiked with E. coli endotoxin at 50,000 endotoxin units per §· of DNA (EU/§·). The DNA was treated with the MiraCLEAN¢ç Kit using three rounds of extraction. Endotoxin levels were decreased by over 2,000 fold.
Endotoxin Á¦°Å¸¦ ÅëÇÑ ´Ü¹éÁú ¹ßÇö Çâ»ó
Figure 2. Plasmid DNA exhibits high transfection efficiency after treatment with the MiraCLEAN
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Kit. COS-7 cells were transfected with 2 §¶ of plasmid DNA and 2 §¡ of the TransIT¢ç-LT1 Reagent in 6-well plates. Cells were harvested at 24 hours post transfection and assayed for luciferase expression. EU=Endotoxin Units
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Transfection È¿À² È®Àοë Label IT
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Plasmid Delivery Control, Cy
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Keyword : V5910,MIR5910,V5900,MIR5900
µ¶¼º Á¦°Å - Transfection È¿À² Áõ°¡
DNA ½Ã·á·Î ºÎÅÍ endotoxin Á¦°Å
MiraCLEAN¢ç Endotoxin Removal Kit