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DNA ½Ã·á·Î ºÎÅÍ endotoxin Á¦°Å

MiraCLEAN¢ç Endotoxin Removal Kit

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Mirus
MIR 5910
MiraCLEAN Endotoxin Removal Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
for 10 §· DNA
°¡°Ý¹®ÀÇ   V5900.pdf
Mirus
MIR 5900
MiraCLEAN Endotoxin Removal Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
for 100 §· DNA
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E.coli Áõ½Ä½Ã ¿À¿°µÈ plasmidÀÇ endotoxinÀ» Á¦°ÅÇÏ´Â kit·Î, 2°³ÀÇ ½Ã¾àÀ¸·Î ±¸¼ºµÇ¾î °£ÆíÇÏ°Ô Á¦°Å °¡´ÉÇÏ´Ù. in vitro, in vivo À¯ÀüÀÚµµÀÔÀ» ½Ç½ÃÇÒ ¶§, plasmid¿¡ È¥ÀÔµÈ endotoxinÀÌ µµÀÔ È¿À²¿¡ ¿µÇâÀ» ÁÖ´Â °ÍÀ¸·Î ¾Ë·ÁÁ®ÀÖÀ¸¸ç, º» Á¦Ç°Àº plasmidÀÇ endotoxinÀ» Á¦°ÅÇÏ¿© ¾ÈÀüÇÏ°í È¿À²ÀûÀ¸·Î À¯ÀüÀÚ µµÀÔÀÌ °¡´ÉÇϵµ·Ï ÇÏ¿´´Ù
MiraCLEAN¢ç Endotoxin Removal Kit ±¸¼º

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EndoGO Extraction Reagent

2.2 ml

MiraCLEAN¢ç Buffer

2 ml

Endotoxin Á¦°Å È¿À²

Figure 1. The MiraCLEAN¢ç Kit Effectively Removes Endotoxin from Crude Plasmid DNA Preparations. Plasmid DNA was spiked with E. coli endotoxin at 50,000 endotoxin units per §· of DNA (EU/§·). The DNA was treated with the MiraCLEAN¢ç Kit using three rounds of extraction. Endotoxin levels were decreased by over 2,000 fold.
Endotoxin Á¦°Å¸¦ ÅëÇÑ ´Ü¹éÁú ¹ßÇö Çâ»ó

Figure 2. Plasmid DNA exhibits high transfection efficiency after treatment with the MiraCLEAN¢ç Kit. COS-7 cells were transfected with 2 §¶ of plasmid DNA and 2 §¡ of the TransIT¢ç-LT1 Reagent in 6-well plates. Cells were harvested at 24 hours post transfection and assayed for luciferase expression. EU=Endotoxin Units
°ü·Ã Á¦Ç°
Transfection È¿À² È®Àοë Label IT¢ç Plasmid Delivery Control, Cy¢ç 3 ÀÚ¼¼È÷º¸±â

Keyword : V5910,MIR5910,V5900,MIR5900

µ¶¼º Á¦°Å - Transfection È¿À² Áõ°¡
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DNA ½Ã·á·Î ºÎÅÍ endotoxin Á¦°Å
MiraCLEAN¢ç Endotoxin Removal Kit