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Home > ÀüÁ¦Ç°º¸±â > Stem Cell > hiPSC/hESC > Xeno-free & GMP DEF-CS culture medium
Xeno-freeÀÇ Human iPS ¼¼Æ÷ / ES ¼¼Æ÷ ¹è¾ç½Ã½ºÅÛ

Xeno-free & GMP DEF-CS culture medium

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Cellartis
Y30045
Cellartis DEF-CS 500 Xeno-Free Culture Medium, w/o antibiotics
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 Kit
(Package)
°¡°Ý¹®ÀÇ   x32558
Cellartis
Y30042
Cellartis DEF-CS 500 Xeno-Free Additives
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 Set
°¡°Ý¹®ÀÇ   x32558


¡Ø º» Á¦Ç°Àº ÀÏ¹Ý ¿¬±¸¿ë ¹èÁö ¿Ü¿¡µµ PIC/SÀÇ GMP °¡À̵å¶óÀο¡ µû¸¥ Ç°ÁúÀÌ º¸ÁõµÈ DEF-CS 500 Xeno-Free GMP ¹è¾ç ¹èÁö¸¦ Á¦°øÇÒ ¼ö ÀÖ½À´Ï´Ù (Custom).
GMP grade Á¦Ç° °ü·Ã ¹®ÀÇ»çÇ×Àº ´ÙÄ«¶óÄÚ¸®¾Æ °í°´Áö¿ø (TEL. 02-2081-2510, Email. support@takara.co.kr)À¸·Î ¿¬¶ô ÁÖ¼¼¿ä.

  • µ¿¹°¡¤»ç¶÷ À¯·¡ ¼ººÐ ºÒÆ÷ÇÔ (Xeno-free)
  • ´ÜÀÏ ¼¼Æ÷ (single cell) »óÅ·Π°è´ë (passaging) °¡´É
  • ¹ÌºÐÈ­ »óÅ¿¡¼­ Àå±â°£ °è´ë °¡´É (´Ù±â´É¼º, ÀÚ°¡Àç»ý, ºÐÈ­´ÉÀÇ ÀáÀç·ÂÀ» Àå±â°£ À¯Áö)
  • ÀüÀÓ»ó¿¡¼­ÀÇ »ç¿ë¿¡ ÃÖÀû
  • Lot °£ÀÇ Â÷ÀÌ°¡ Àû¾î ¾ÈÁ¤ÀûÀÎ iPS ¹è¾çÀÌ °¡´É
Á¦Ç°¼³¸í
º» Á¦Ç°Àº µ¿¹°¡¤»ç¶÷ À¯·¡ ¼ººÐÀÌ Æ÷ÇÔµÇÁö ¾ÊÀº chemically defined mediaÀÌ´Ù. Feeder-free ¹è¾ç Á¶°Ç ÇÏ¿¡¼­ Àå±â°£ ¹ÌºÐÈ­ »óŸ¦ À¯ÁöÇÑ »óÅ·ΠȿÀ²ÀûÀÎ »ç¶÷ iPS¼¼Æ÷ÀÇ °è´ë¹è¾çÀÌ °¡´ÉÇÏ´Ù. ³ôÀº Áõ½Ä·üÀ» ¾òÀ» ¼ö ÀÖ°í, ÇÙÇü (karyotype) ¶ÇÇÑ À¯ÁöµÈ´Ù. ÀÌ ½Ã½ºÅÛÀº ±âÁ¸ÀÇ DEF-CS¢â 500 ¹è¾ç ½Ã½ºÅÛó·³ ÃÖÀûÀÇ »óÅ·Π´ë·® ¹è¾çÀÌ °¡´ÉÇÏ´Ù. ÀÌ ¹è¾ç ½Ã½ºÅÛ¿¡¼­ Áõ½ÄÇÑ ¼¼Æ÷´Â ¹ÌºÐÈ­ »óŸ¦ À¯ÁöÇÏ¿© ºÐÈ­µÈ ¼¼Æ÷°¡ °ÅÀÇ °ËÃâµÇÁö ¾ÊÀ¸¹Ç·Î, º°µµÀÇ ºÐÈ­¼¼Æ÷ Á¦°Å ÀÛ¾÷ÀÌ ÇÊ¿ä ¾ø´Ù. ¶ÇÇÑ ´ÜÀϼ¼Æ÷ »óÅ·Π¹è¾çÇÒ ¼ö Àֱ⠶§¹®¿¡ iPS¼¼Æ÷ Ŭ·Î´×¿¡ ÀÌ¿ëÇϰųª, °è´ë ½Ã ÇÊ¿äÇÑ ÄÝ·Î´Ï ºÐ»êÀÛ¾÷¿¡ µû¸¥ ¼¼Æ÷¿¡ ¹ÌÄ¡´Â ¿µÇâÀ» ÃÖ¼ÒÈ­ ÇÒ ¼ö ÀÖ´Ù.

iPS¼¼Æ÷, ES¼¼Æ÷ÀÇ ¹è¾ç ½Ã, º°µµÀÇ Ç÷¹ÀÌÆ® ÄÚÆÃÁ¦°¡ ÇÊ¿äÇÏ´Ù. Ç÷¹ÀÌÆ® ÄÚÆÃÁ¦·Î´Â iMatrix-511 (Code T303) ¶Ç´Â Corning Synthemax II-SC Substrate (Corning, Cat. No. 3535)¸¦ ±ÇÀåÇÑ´Ù.

º» Á¦Ç°Àº ¸ðµÎ Ç×»ýÁ¦¸¦ Æ÷ÇÔÇÏÁö ¾Ê°í ÀÖÀ¸¸ç, GMP grade Á¦Ç°Àº º°µµÀÇ ¹èÁö÷°¡Á¦(additives)°¡ ÇÊ¿äÇÏ´Ù.
Application
  • ÀüÀÓ»ó¿¡¼­ »ç¶÷ iPS¼¼Æ÷ÀÇ scale-up°ú ´ë·® ¹è¾ç
  • ½Å¾à°³¹ß ½ºÅ©¸®´×¿ë Á¶Á¦¹èÁö·Î »ç¿ë
  • Reprogramming, ºÐÈ­À¯µµ¿ë ¹èÁö·Î »ç¿ë
³»¿ë

Cellartis¢ç DEF-CS¢â 500 Xeno-Free Culture Medium w/o antibiotics (Code Y30045)

Cellartis¢ç DEF-CS¢â 500 Xeno-Free Basal Medium w/o antibiotics

500 ml

Cellartis¢ç DEF-CS¢â 500 Xeno-Free additives

1 Set; ¹èÁö ÷°¡Á¦
(Code Y30042)

Cellartis¢ç DEF-CS¢â Xeno-Free Additive 1

500 §¡

Cellartis¢ç DEF-CS¢â Xeno-Free Additive 2

200 §¡


¡Ø º°µµÀÇ Ç÷¹ÀÌÆ® ÄÚÆÃÁ¦°¡ ÇÊ¿äÇÕ´Ï´Ù.
±ÇÀå Ç÷¹ÀÌÆ® ÄÚÆÃÁ¦
iMatrix-511 (Code T303)
Corning Synthemax II-SC Substrate£¨Corning, Cat. No. 3535£©
¡Ø Cellartis DEF-CS 500 Xeno-Free GMP Grade Basal Medium(Prototype)(Code Y30071)¿¡´Â º°µµ ¹èÁö ÷°¡Á¦°¡ ÇÊ¿äÇÕ´Ï´Ù.
¼ö¼Û
Cellartis¢ç DEF-CS¢â 500 Xeno-Free Basal Medium£º2¢¦8 ¡É
Cellartis¢ç DEF-CS¢â Xeno-Free Additives£º£­15 ¡É ÀÌÇÏ
º¸Á¸
Cellartis¢ç DEF-CS¢â 500 Xeno-Free Basal Medium w/o Antibiotics : 4¡É
Cellartis¢ç DEF-CS¢â 500 Xeno-Free Additives£º-15¡É ÀÌÇÏ
Application data

±×¸² 1. Ç÷¹ÀÌÆ® ÄÚÆÃÁ¦ 2Á¾ Synthemax II, iMatrix-511ÀÇ »ç¿ë µ¥ÀÌÅÍ
º» Á¦Ç°À» »ç¿ëÇÏ¿© »ç¶÷ iPS¼¼Æ÷¸¦ Ç÷¹ÀÌÆ® ÄÚÆÃÁ¦ÀÎ Corning Synthemax II-SC Substrate (Corning, Cat. No. 3535) ¹× iMatrix-511 (Á¦Ç°ÄÚµå: T303) »ó¿¡¼­ °¢°¢ ¹è¾çÇÏ°í doubling timeÀ» ºñ±³Çß´õ´Ï ¸ðµÎ ¾ÈÁ¤µÈ ³ôÀº ¼ºÀå·üÀ» º¸¿´´Ù. µû¶ó¼­ º» Á¦Ç°¿¡¼­ ¹è¾ç ½Ã »ó±â 2°¡Áö Ç÷¹ÀÌÆ® ÄÚÆÃÁ¦¸¦ ÃßõÇÑ´Ù.
(28~30½Ã°£ °£°ÝÀ¸·Î 10ȸ ÀÌ»óÀÇ °è´ë¸¦ ½ÃÇàÇÏ¿´´Ù. 2ȸ ½Ç½ÃÇÑ ½ÃÇè°£ÀÇ Æò±ÕÀ» ³ªÅ¸³½´Ù.)


±×¸² 2. ¹ÌºÐÈ­ »óÅ¿¡¼­ Àå±â°£ ¹è¾ç °¡´É
º» Á¦Ç°À» »ç¿ëÇÏ¿© »ç¶÷ iPS¼¼Æ÷ÀÇ °è´ë¸¦ 10ȸ ½ÃÇàÇÑ µÚ, ¹ÌºÐÈ­´É »óÅ°¡ À¯ÁöµÇ´Â °ÍÀ» ¹ÌºÐÈ­ Ç¥ÁöÀÎÀÚÀÎ Oct-4¿Í SSEA4¿¡ ´ëÇÑ ¸é¿ª¿°»öÀ¸·Î È®ÀÎÇÏ¿´´Ù. Ç÷¹ÀÌÆ® ÄÚÆÃÁ¦´Â Corning Synthemax II-SC Substrate (Corning, Cat. No. 3535)¸¦ »ç¿ëÇÏ¿´´Ù.


±×¸² 3. Àå±â°£ °è´ë ÈÄ¿¡µµ ´ÙºÐÈ­´É À¯Áö
º» Á¦Ç°À» »ç¿ëÇÏ¿© »ç¶÷ iPS¼¼Æ÷ÀÇ °è´ë¸¦ 10ȸ ½ÃÇàÇÑ µÚ, »ï¹è¿±À¸·ÎÀÇ ºÐÈ­´É·ÂÀÌ À¯ÁöµÇ´Â °ÍÀ» °¢ ¹è¿± ƯÀÌÀû Ç×ü·Î ¸é¿ª¿°»ö (Á߹迱Àº ASMA, ¿Ü¹è¿±Àº ¥âIII-tubulin, ³»¹è¿±Àº SOX17°ú HNF4a·Î °ËÃâ)À» ½Ç½ÃÇÏ¿© È®ÀÎÇÏ¿´´Ù. ¾Æ·¡ ±×¸²ÀÇ ÇÙ ¿°»öÀº DAPI¸¦ »ç¿ëÇÏ¿´´Ù. ¹è¾ç ½Ã Ç÷¹ÀÌÆ® ÄÚÆÃÁ¦´Â Corning Synthemax II-SC Substrate (Corning, Cat. No. 3535)¸¦ »ç¿ëÇÏ¿´´Ù.
°ü·Ã ¿Â¶óÀÎ °¡À̵å
Cellartis Áٱ⼼Æ÷ ¿¬±¸¿ë Á¦Ç° ¼±Åà °¡À̵å
http://takara.co.kr/web01/product/productList.asp?lcode=D150727

Keyword : Y30010,Áٱ⼼Æ÷¹èÁö,monolayerculture,singlecellculture,GMP,Clinical

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