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Home > ÀüÁ¦Ç°º¸±â > Genome Editing > CRE recombinase > CRE Recombinase Gesicles

CRE Recombinase Gesicles

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Clontech
631449
Cre Recombinase Gesicles
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200 uL
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11.01 ~ 12.27
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CRE Recombinase Gesicles¸¦ ÀÌ¿ëÇÏ¿© Æ÷À¯·ù ¼¼Æ÷¿¡ ÀçÁ¶ÇÕ CRE ´Ü¹éÁúÀ» plasmid³ª virus¸¦ ÀÌ¿ëÇÏÁö ¾Ê°í ºü¸£°í È¿°úÀûÀ¸·Î Àü´Þ ÇÒ ¼ö ÀÖ´Ù. Gesicles´Â exosome-like vesiclesÇüÅ·Π³óÃàµÈ ÀçÁ¶ÇÕ CRE ´Ü¹éÁúÀ» ¸ñÀû ¼¼Æ÷·Î ¿î¹ÝÇÑ´Ù. ¼¼Æ÷³»·Î µµÀÔµÈ ÀçÁ¶ÇÕ CRE ´Ü¹éÁú¿¡´Â nuclear localization signalÀ» º¸À¯ÇÏ°í ÀÖ¾î ¼¼Æ÷ÇÙÀ¸·Î À̵¿ÇÏ¿© LoxP sitesÀÇ CRE-mediated recombination°¡ °¡´ÉÇϵµ·Ï ÇÑ´Ù.
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  • Flox your cells on demand: CRE recombinase is present when you need it and gone when you don't.
  • Super efficient: CRE Recombinase Gesicles are faster and more efficient than plasmid transfection.
  • Extremely simple protocol: Just apply 10-20 ul of CRE Recombinase Gesicles to your cells. That's it!
  • Limit unintended recombination events: Since there is no coding gene present, there is no risk of sustained CRE recombinase expression in your target cells.
What are the Benefits of Gesicle Technology?
  • CRE Recombinase Gesicles are far simpler to use than either plasmid or viral gene delivery. Simply apply 10-20 ul of the gesicles to your target cells; no preparation or pre-treatment is required.
  • CRE Recombinase Gesicles can be used with any cell type, including dividing and non-dividing cells, primary cells, and cell lines.
  • CRE Recombinase Gesicles ensure efficient delivery and high activity of CRE recombinase (see Images and Data tab). When using gesicle delivery there is no delay for transcription/translation: the protein is present and active as soon as you apply the gesicles to your cells.
  • Since there is no coding gene present, there is no risk of sustained CRE recombinase expression in your target cells. CRE recombinase is present when you need it and gone when you don't.
Applications
  • Deliver CRE recombinase for gene editing studies
  • Limit and control site-specific recombination events including deletions, insertions, translocations and inversions

±×¸² 1. Delivery of CRE recombinase into mammalian cells using gesicles. CRE Recombinase Gesicles are exosome-like vesicles that, when applied to your target cells, fuse with the plasma membrane and discharge CRE recombinase into the cell. CRE recombinase is transported to the nucleus via a nuclear localization signal where it can carry out CRE-mediated recombination between LoxP sites (top panel). Data was generated using a reporter cell line in which the LacZ gene is only expressed when the upstream, lox-flanked stop codon is excised by CRE recombinase (bottom panel, left and center images). CRE Recombinase Gesicles are labeled with the CherryPicker fluorescent protein, so treated target cells can be easily visualized by fluorescent microscopy (bottom panel, right hand image).


±×¸² 2. Rapid and efficient genome modification using CRE Recombinase Gesicles. A cell line harboring an integrated, loxP-conditional LacZ expression cassette (top panel) was either transfected for 6 hr with a plasmid expressing CRE recombinase (bottom panel, middle image) or treated with 20 ¥ìl CRE recombinase-containing gesicles for 3 hr (bottom panel, right image). 24 hr after treatment, cells were stained for LacZ expression using the Beta-Galactosidase Staining Kit from Clontech (Cat. No. 631780). The Beta Galactosidase gene is only expressed when the upstream loxP-flanked stop codon is excised by CRE recombinase. Results showed that, compared to plasmid transfection delivery of CRE recombinase, using gesicles resulted in faster and more efficient excision.




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