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Gene editing
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IT-X2¢ç Transfection Reagent
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IT-X2¢ç Transfection Reagent
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Mirus
MIR 6003
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IT-X2 Dynamic Delivery System
0.3 ml
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11.01 ~ 12.27
Mirus
MIR 6004
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IT-X2 Dynamic Delivery System
0.75 ml
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11.01 ~ 12.27
Mirus
MIR 6000
Trans
IT-X2 Dynamic Delivery System
1.5 ml
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11.01 ~ 12.27
Mirus
MIR 6005
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IT-X2 Dynamic Delivery System
1.5 ml¡¿5
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11.01 ~ 12.27
Mirus
MIR 6006
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IT-X2 Dynamic Delivery System
1.5 ml¡¿10
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11.01 ~ 12.27
Product index
Overview
RetroNectin¢ç - Viral ..
[FAQ] Retronectin
RetroNectin¢çÀÇ ´Ù¾çÇÑ..
T cell ¿¬±¸ÀÚ¸¦ À§ÇÑ ..
[Cancer Research] Immu..
Cancer ¿¬±¸ °¡À̵å
T cell therapy ¿¬±¸
RetroNectin¢ç (Recombi..
Anti-CD3 mAb GMP grade
LymphoONE¢ç T-Cell Exp..
Cytokine GoStix¢â Plus..
GT-T551 Culture medium
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Gene editing
Recombinant Cas9 ´Ü¹é..
sgRNA ÇÕ¼º°ú ½ºÅ©¸®´×
TransIT-X2¢ç Transfect..
[Upgrade] Guide-it¢â L..
TCR/BCR Profiling
Mouse TCR a/b Profilin..
Mouse BCR IgG H/K/L Pr..
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Cell culture ÇʼöÇ° - ..
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MycoAlert PLUS mycopla..
MycoAlert Mycoplasma D..
MycoZap Antibiotics
MycoZap Mycoplasma Eli..
TaKaRa PCR Mycoplasma ..
CycleavePCR Mycoplasma..
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Phosphate Buffered Sal..
TBS(Tris-Buffered Sali..
´Ù¾çÇÑ cell type°ú ´Ù¾çÇÑ application¿¡ °íÈ¿À² Àû¿ë °¡´É (±×¸² 1)
DNA¿Í siRNA/miRNA, co-transfection ¸ðµÎ °¡´É
Primary cellÀ» Æ÷ÇÔÇÑ ´Ù¾çÇÑ ¼¼Æ÷¿¡ È¿À²ÀûÀ¸·Î µµÀÔ:
ÁÖ¿ä cell lineº° ½ÇÇè °¡À̵å¶óÀÎ º¸±â (Ŭ¸¯)
Animal origin Free
CRISPR/Cas9 ½ÇÇè¿¡µµ ÃÖÀû: Cas9-sgRNA Á¶ÇÕ¿¡ µû¸¥ ½ÇÇè °¡À̵å¶óÀÎ Á¦°ø
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IT-X2
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for CRISPR RNP Delivery (
PDF ¸µÅ©
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IT-X2
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for CRISPR RNP + ssODN Delivery Protocol (
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IT-X2
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for CRISPR pDNA/gRNA Delivery Protocol (
PDF ¸µÅ©
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-
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IT-X2
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for CRISPR Plasmid Delivery Protocol (
PDF ¸µÅ©
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IT
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2000 Transfection Reagent¸¦ »ç¿ëÇؼ luciferase¸¦ encodingÇÏ´Â plasmid DNA¸¦ 41°³ÀÇ ´Ù¸¥ cell type¿¡ 3 : 1 (reagent : DNA)ÀÇ ºñÀ²·Î transfection ÇÏ¿´´Ù. Transfection 24½Ã°£ ÈÄ¿¡ standard luciferase assay¸¦ ÁøÇàÇÑ °á°ú
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Dynamic Delivery SystemÀº non-liposomal polymeric ½Ã½ºÅÛÀ¸·Î primary cellÀ» Æ÷ÇÔÇÑ ´Ù¾çÇÑ cell type¿¡¼ °íÈ¿À² transfectionÀÌ °¡´ÉÇÏ´Ù. ÀÌ Á¦Ç°À» »ç¿ëÇϸé DNA, siRNA/RNAi ¹× DNA¿Í siRNA µîÀÇ co-transfectionÀ» ÁøÇàÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ serum compatible transfectionÀÌ Æ¯Â¡À¸·Î transfection ÈÄ º°µµÀÇ media change°¡ ÇÊ¿äÇÏÁö ¾Ê´Ù´Â ÀåÁ¡ÀÌ ÀÖ´Ù.
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Snap Assembly Master Mix (Code 638947)
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TaKaRa PCR Mycoplasma Detection Set
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CycleavePCR Mycoplasma Detection Kit
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MycoAlert PLUS mycoplasma detection kit
- RNP µµÀÔµµ °¡´ÉÇÑ:
Guide-it¢â Recombinant Cas9 (Electroporation-Ready)
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Guide-it¢â Complete sgRNA Screening System
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Dynamic Delivery System.
A549 (A)¿Í MDCK (B) ¼¼Æ÷¿¡ luciferase¸¦ encodingÇÏ´Â plasmid¸¦
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2000 (Thermo Fisher Scientific), Lipofectamine
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3000 (Thermo Fisher Scientific)À¸·Î °¢°¢ÀÇ ÃÖÀû Á¶°Ç¿¡¼ 24½Ã°£ µ¿¾È transfection ÇÑ °á°úÀÌ´Ù. Luciferase activity·Î transfection È¿À²À» ÃøÁ¤ÇÏ°í, ¼Õ»óµÈ ¼¼Æ÷ÀÇ ¼¼Æ÷Áú¿¡¼ ¹æÃâµÇ´Â LDH¸¦ Á¤·®ÇØ ¼¼Æ÷µ¶¼ºÀ» Æò°¡ÇÏ¿´´Ù. ±× °á°ú Lipofectamine
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Dynamic Delivery System·Î transfection ÇÑ °á°úÀÌ´Ù. 96-well plate¿¡¼ 0.2~0.4 §¡ÀÇ
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·Î 0.1 §¶ÀÇ DNA¸¦ transfection Çß°í (2:1, 3:1, 4:1 / reagent: DNA ratio), 48½Ã°£ µÚ 3¹Ýº¹À¸·Î guava easyCyte¢â 5HT Flow Cytometer¿¡¼ ºÐ¼®µÇ¾ú´Ù. ±× °á°ú
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Dynamic Delivery SystemÀº ´Ù¾çÇÑ cell-line ¹× primary cell¿¡¼ °íÈ¿À²·Î GFP¸¦ ¹ßÇö½Ãų ¼ö ÀÖ¾ú´Ù.
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Dynamic Delivery System.
HEK293T/17°ú U2OS ¼¼Æ÷¿¡
2-part gRNA (IDT)¸¦ Ÿ°ÙÇÏ´Â PPIB (cyclophilin B)¿Í Cas9 protein (PNA Bio)À¸·Î Á¦ÀÛÇÑ Ribonucleoprotein (RNP) complexes¸¦
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Dynamic Delivery System (1 §¡/well) ¶Ç´Â Lipofectamine
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CRISPRMAX¢â (1.5 §¡/well and 1 §¡/well of Lipofectamine
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Cas9 Plus¢â Reagent, ThermoFisher), Lipofectamine
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RNAiMAX (1.5 §¡/well), Lipofectamine
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3000 (1.5 ¥ìl/well and 1 ¥ìl/well of P3000¢â Reagent, ThermoFisher)·Î Á¦Á¶»çÀÇ ÇÁ·ÎÅäÄÝ¿¡ µû¶ó transfection ÇÑ °á°úÀÌ´Ù. 6 nM Cas9 protein (PNA Bio)°ú ´Ù¾çÇÑ ¾çÀÇ gRNA (6 nM, 12 nM)·Î Å×½ºÆ®µÇ¾ú°í, transfection 48½Ã°£ ÈÄ T7E1 mismatch detection assay·Î cleavage efficiency¸¦ ÃøÁ¤ÇÏ¿´´Ù. ±× °á°ú
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Dynamic Delivery SystemÀº Ÿ»ç ´ëºñ ³ôÀº È¿À²·Î RNP¸¦ µµÀÔÇÏ¿© °íÈ¿À²·Î À¯ÀüÀÚ ÆíÁýÀ» ÁøÇàÇÒ ¼ö ÀÖ¾ú´Ù.
Keyword :
Gene editing
Recombinant Cas9 ´Ü¹éÁú
sgRNA ÇÕ¼º°ú ½ºÅ©¸®´×
Trans
IT-X2¢ç Transfection Reagent
À¯ÀüÀÚ »ðÀÔÀ» À§ÇÑ donor template ÇÕ¼º (5 kb..
[Upgrade] Guide-it¢â Long ssDNA Production System v2