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Home > ÀüÁ¦Ç°º¸±â > Epigenetics > DNA Methlyation ¿¬±¸ > TB Green¢ç Premix Ex Taq¢â GC (Perfect Real Time)
genomic DNA ¶Ç´Â CpG island ¿µ¿ªÀÇ ÁõÆø¿¡ ÃÖÀû

TB Green¢ç Premix Ex Taq¢â GC (Perfect Real Time)

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Takara
RR071A
TB Green¢ç Premix Ex Taq¢â GC (Perfect Real Time)
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200 ȸ
(50 §¡ ¹ÝÀÀ°è)
321,000¿ø  RR071_v1901.pdf


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  • CpG island¸¦ Æ÷ÇÔÇÑ GC rich¿µ¿ªÀÇ Çؼ®¿¡µµ Ãßõ
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TB Green¢ç Premix Ex Taq¢â GC (Perfect Real Time)´Â TB Green¢çÀ» ÀÌ¿ëÇÑ intercalating ¹ý Real Time PCR Àü¿ë ½Ã¾àÀÌ´Ù. 2¡¿ ³óµµÀÇ premix type½Ã¾àÀ¸·Î, ½Ç½Ã°£ ¸ð´ÏÅ͸µ¿¡ ÀûÇÕÇÑ ³óµµÀÇ TB Green¢çÀ» ¹Ì¸® Æ÷ÇÔÇÏ°í ÀÖ¾î ¹ÝÀÀ ¾×ÀÇ Á¶Á¦°¡ °£´ÜÇÏ´Ù.
º» Á¦Ç°Àº ´ÙÄ«¶óÀÇ µ¶ÀÚÀûÀΠ÷°¡Á¦¿Í ¹ÝÀÀ Á¶¼ºÀÇ °³·®À¸·Î GC ÇÔ·® 60-70%ÀÇ GC rich ¿µ¿ª¿¡ ´ëÇÑ ¹ÝÀÀ¼º°ú Á¤·®¼ºÀÌ Å©°Ô Çâ»óµÇ¾ú´Ù (ÁõÆø»çÀÌÁî: ~200 bp ±ÇÀå). º» Á¦Ç°À» ÀÌ¿ëÇÔÀ¸·Î½á CpG island¸¦ Æ÷ÇÔÇÑ ¿µ¿ª µî GC rich Ÿ°Ù¿¡¼­µµ Á¤¹ÐÇÑ Çؼ®ÀÌ °¡´ÉÇÏ´Ù. GCÇÔ·® 60% ÀÌÇÏÀÇ ÀϹÝÀûÀΠŸ°Ù¿¡¼­µµ ¹ÝÀÀ¼ºÀÌ ¾çÈ£ÇϹǷÎ, µ¿ÀÏÇÑ PCR Á¶°ÇÀ¸·Î Á¤·®ºÐ¼® ÇÒ ¼ö ÀÖ´Â ÀåÁ¡ÀÌ ÀÖ´Ù.
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TB Green¢ç Premix Ex Taq¢â GC (Perfect Real Time) (Code RR071A, 50 §¡ ¹ÝÀÀ½Ã 200ȸ ºÐ·®)

1.

TB Green¢ç Premix Ex Taq¢â GC (Perfect Real Time) (2¡¿conc.)*1

1 §¢¡¿ 5°³

2.

ROX Reference Dye (50¡¿conc.)*2

200 §¡

3.

ROX Reference Dye II (50¡¿conc.)*2

200 §¡


*1 TaKaRa Ex Taq HS, dNTP Mixture, Mg2£« ¹× TB Green¢çÀ» Æ÷ÇÔÇÑ´Ù.
*2 Life TechnologiesÀÇ Real Time PCR ÀåÄ¡ µî well °£ Çü±¤ ½ÅÈ£¸¦ º¸Á¤ÇÏ´Â ÀåÄ¡·Î Çؼ®ÇÏ´Â °æ¿ì¿¡ »ç¿ëÇÑ´Ù.
  • ROX Reference Dye(50¡¿)À» ÷°¡ÇÏ´Â ±âÁ¾
    Applied Biosystems 7300 Real-Time PCR System (Life Technologies)
    StepOnePlus Real-Time PCR System (Life Technologies)
  • ROX Reference Dye II(50¡¿)À» ÷°¡ÇÏ´Â ±âÁ¾
    Applied Biosystems 7500/7500 Fast Real-Time PCR System (Life Technologies)
  • ÷°¡°¡ ÇÊ¿ä ¾ø´Â ±âÁ¾
    CronoSTAR¢â 96 Real-Time PCR System (4ch) (Code 640231)
    Thermal Cycler Dice Real Time System III (Code TP950/TP970/TP980)
    Thermal Cycler Dice Real Time System II (Code TP900/TP960)
    Thermal Cycler Dice Real Time System Single (Code TP850/TP870)
    Thermal Cycler Dice Real Time System Lite (Code TP700/TP760)
    Smart Cycler System/Smart Cycler II System (Cepheidȍ)
    LightCycler 96/LightCycler 480 System (Roche Diagnosticsȍ)
    CFX96 Real-Time PCR Detection System (Bio-Radȍ)
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Keyword :

DNA Methlyation ¿¬±¸
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TaKaRa EpiTaq HS (for bisulfite-treated DNA)
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ƯÁ¤ ¿µ¿ªÀÇ DNA MethylationÀ» Çؼ® °¡´É
EpiScope¢ç MSP Kit
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genomic DNA ¶Ç´Â CpG island ¿µ¿ªÀÇ ÁõÆø¿¡ ÃÖ..
TB Green¢ç Premix Ex Taq¢â GC (Perfect Real Time)
>
DNA ¸ÞÆ¿È­ Çؼ® ÄÁÆ®·Ñ
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