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Epigenetics
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Epigenetics¸¦ À§ÇÑ Cloning Kit
> Mighty TA-cloning Kit
°íÈ¿À²ÀÇ Ligation premix ¿Í T-vectorÀÇ Á¶ÇÕ
Mighty TA-cloning Kit
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Takara
6028
Mighty TA-cloning Kit
20 ȸ
178,000¿ø
Product index
Overview
[¼±Åð¡À̵å] Epigeneti..
[°³¿ä] ChIP-Seq Librar..
Cancer ¿¬±¸ °¡À̵å
DNA Methlyation ¿¬±¸
Metly-sensitive/non-se..
TaKaRa EpiTaq HS (for ..
EpiScope¢ç MSP Kit
TB Green¢ç Premix Ex T..
¸ÞÆ¿È/ºñ¸ÞÆ¿È ÄÁÆ®·Ñ..
McrBC
Methylated DNA ºÐ¸®¿Í ºÐ¼®
EpiXplore Methylated D..
Meth-Seq DNA Enrichmen..
Chromatin ¸é¿ª ħ°¨ (ChIP)
Micrococcal Nuclease
Anti-Histone monoclona..
EpiXplore ChIP Assay K..
DNA Fragmentation Kit
ChIP Elute Kit
EpiScope¢ç Nucleosome ..
Capturem¢â IP £¦ Co-IP..
ChIP-seq, Cut&Run-seq (NGS..
[Àû¿ë] ¼Ò·®¼¼Æ÷·ÎºÎÅÍ ..
[Customer Spotlight] C..
[DNA-Seq ±â¼ú°³¿ä] Thr..
[ChIP-seq ³í¹®¸®½ºÆ®] ..
SMARTer¢ç ThruPLEX¢ç D..
DNA SMART ChIP-Seq Kit
SMARTer¢ç DNA index ki..
miRNA ¹ßÇö ¡¤ Á¤·®
[Cancer Research] Canc..
MicroRNA ¿¬±¸¿ë Á¦Ç° ..
MicroRNA qRT-PCR Á¤·®
Tet Inducible miRNA ¹ß..
Small RNA-Seq Library ..
MicroRNA-Seq Library P..
miRNA ¹ßÇö vector (Çü..
Epigenetics¸¦ À§ÇÑ Cloning..
Online In-Fusion Tools
Mighty TA-cloning Kit
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Taq
±â¹ÝÀÇ DNA polymerase·Î PRCÇÑ »ê¹°ÀÇ TA-cloningÀ» À§ÇÑ Á¦Ç°ÀÌ´Ù. °íÈ¿À² ligation premixÀÎ
DNA Ligation Kit £¼Mighty Mix£¾ (Code: 6023)
¿Í pUC °è¿ÀÇ pMD20-T vector¸¦ Æ÷ÇÔÇÏ°í ÀÖ¾î ºü¸£°í °£ÆíÇÏ°Ô ligationÇÒ ¼ö ÀÖ´Ù.
*TA cloning:
Taq
DNA polymeraseÀÇ PCR »ê¹°Àº ±× 3¡¯ ¸»´Ü¿¡ deoxyriboadenosine (dA) ÇÑ ¿°±â°¡ Ãß°¡µÈ´Ù. ÀÌ PCR ÁõÆø»ê¹°Àº 3¡¯ ¸»´Ü¿¡ deoxyribothymidine (dT) ÇÑ°³°¡ Ãß°¡µÈ T vector°¡ PCR ÁõÆø»ê¹°ÀÇ dA ¿°±â¿Í »óº¸ÀûÀ¸·Î °áÇÕÇÏ´Â °ÍÀ» ÀÌ¿ëÇØ cloningÇÒ ¼ö ÀÖ´Ù.
[ÁÖÀÇ] PrimeSTAR
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½Ã¸®ÁîÀÇ PCR »ê¹°Àº ´ëºÎºÐ ÆòÈ° ¸»´Ü (blunt end)ÀÌ µÇ±â ¶§¹®¿¡ ¸»´Ü¿¡ dA ¿°±â¸¦ 1°³ºÎ°¡ÇÏ´Â °úÁ¤À» º°µµ·Î ÁøÇàÇؾ߸¸ ÀÌ Á¦Ç°À» »ç¿ëÇÒ ¼ö ÀÖ´Ù. µû¶ó¼ ÀÌ °úÁ¤ÀÇ ½Ã¾àÀ» Æ÷ÇÔÇÑ
Mighty TA-cloning Reagent Set for PrimeSTAR
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(Code 6019)
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pMD20-T vector (50 ng/§¡)
20 §¡
Ligation Mighty Mix
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2 X 50 §¡
Positive Control Insert
*2
10 §¡
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DNA Ligation Kit £¼Mighty Mix£¾(Code 6023)
¿¡ Æ÷ÇÔµÈ ½Ã¾à°ú µ¿ÀÏ
*2
3¡¯ ¸»´Ü¿¡ dA overhangÀ» °¡Áö´Â ¾à 200 bpÀÇ DNA fragment
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E.coli
gDNA¸¦ ÁÖÇüÀ¸·Î
TaKaRa Ex Taq
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»ç¿ë»óÀÇ ÁÖÀÇ
1. Ligation Mighty Mix´Â ¾óÀ½¿¡¼ õõÈ÷ ³ìÀÎ µÚ »ç¿ëÀü °¡º±°Ô ¼¯¾îÁØ´Ù.
2. Transformation ÈÄ¿¡µµ cloningÇÑ insert¿¡ ±æÀ̳ª ¹æÇâ¿¡ µû¶ó LB + Amp/X-Gal/IPTG plates¿¡¼ ÆĶõ»ö colony°¡ Çü¼ºµÉ ¼ö ÀÖ´Ù. ÀÌ °æ¿ì colony PCRÀ» ÅëÇØ insert DNAÀÇ ¿©ºÎ¸¦ È®ÀÎÇÑ´Ù.
3. Agarose gel¿¡¼ ¸ñÀû PCR »ê¹°À» ȸ¼öÇÏ´Â °æ¿ì UV¿¡ ¿À·¡ Á¶»çµÇ¸é DNA°¡ ¼Õ»óµÇ¾î cloning È¿À²ÀÌ ³·¾ÆÁú ¼ö ÀÖ´Ù.
4. PCR templateÀ¸·Î clone vector¿Í µ¿ÀÏÇÑ selection marker (pMD20-T vectorÀÇ °æ¿ì Ampr)¸¦ °¡Áø plasmid¸¦ »ç¿ëÇÏ´Â °æ¿ì, template plasmid°¡
E.coli
¿¡ transformation µÇ´Â °ÍÀ» ¹æÁöÇϱâ À§ÇØ Àü±â¿µµ¿ ÈÄ agarose gel¿¡¼ ȸ¼ö ¹× Á¤Á¦ÇÑ´Ù.
pMD20-T vector
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- PrimeSTAR
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µî High fidelity È¿¼Ò¸¦ ÁõÆø»ê¹°ÀÇ TA-cloning
Mighty TA-cloning Reagent Set for PrimeSTAR
¢ç
(Code 6019)
- È¿À² ³ôÀº TA-cloningÀ» À§ÇÑ T-vector
T-Vector pMD20 (Code 3270)
Âü°íÀÚ·á
-
Cloning ½ÇÇè ÀÔ¹®ÀÚ¸¦ À§ÇÑ ¡®Step by Step Cloning °¡À̵塯
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½ÇÇè ¸ñÀûº°·Î ÃßõÇÏ´Â Cloning Kit
Keyword :
Epigenetics¸¦ À§ÇÑ Cloning Kit
Online In-Fusion Tools
°íÈ¿À²ÀÇ Ligation premix ¿Í T-vectorÀÇ Á¶ÇÕ
Mighty TA-cloning Kit