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PPiLight Inorganic Pyrophosphate Assay

제조사	제품코드	제품명	용량	가격 (부가세별도)	비고	사용자매뉴얼
Lonza	LT07-610	PPiLight Inorganic Pyrophosphate Assay	500 tests	가격문의

Product index

면역검사관련제품
- TaKaRa DAB Substrate
- TaKaRa POD Conjugate시..
EIA Kit
OsteoㆍAdipo assay
세포증식 · 세포장해 측정
Apoptosis
- ApopLadder Ex™
- In situ Apoptosis Dete..
Signal Transduction
- ExpressHyb Hybridizati..
항체

제품설명

Inorganic pyrophosphate (also referred to as diphosphate, pyrophosphoric acid or PPi) is a small diphosphate molecule that is required as a substrate or is the product formed from a number of different enzymatic reactions. Enzymes that utilize PPi as a substrate may include phosphotransferases (EC 2.7) and pyrophosphatases (EC 3.6.1.1). Enzymes that generate PPi are more numerous and may include cyclases (EC 4.6.1), hydrolyses (EC 3) and ligases (EC 6).

The PPiLight™ Assay is a non-radioactive bioluminescent assay for the detection of inorganic pyrophosphate. In the presence of PPi the detection reagent catalyses the conversion of AMP to ATP. The assay uses luciferase, which produces light from the newly formed ATP and luciferin.

Figure 1: Bioluminescent reaction

특장점

Fast - Measure enzyme activity via pyrophosphate consumption in 1 hour
Simple - The 2 step, luminescent assay doesn’t require radioactive substrates, beads, detection antibodies or modified substrates
Wide Detection Range - Sensitive to 0.02 μM with a linear range to 10 μM
Versatile - Scalable to 96-, 384-, 1586-well formats

Applications

Measure activity of:

Phosphotransferases
Pyrophosphotases
Cyclases
Ligases
Hydrolases
DNA Polymerases

보존

2°C - 8°C

Keyword :

OsteoㆍAdipo assay

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