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ÀüÁ¦Ç°º¸±â
>
Cloning °ü·Ã
>
¼ö½ÄÈ¿¼Ò£¯DNA Polymerase¡¢RNA Polymerase
> SP6 RNA Polymerase
Cloned
SP6 RNA Polymerase
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Takara
2520A
SP6 RNA Polymerase
3,000 U
174,400¿ø
218,000¿ø
03.02 ~ 04.30
Takara
2520B
SP6 RNA Polymerase
15,000 U
981,000¿ø
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¼ö½ÄÈ¿¼Ò£¯Ligase
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DNA-OFF¢â
Proteinase K
Westase (È¿¸ð ¼¼Æ÷º® ..
Yatalase (°è»ó±Õ ¼¼Æ÷..
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Çü»ó
10 mM
Potassium Phosphate (pH7.9)
1 mM
DTT
0.1 mM
EDTA
150 mM
NaCl
50%
Glycerol
º¸Á¸ -20¡É
÷ºÎ Buffer Á¶¼º (10¡¿)
400 mM
Tris-HCl (pH7.5)
60 mM
MgCl
2
20 mM
Spermidine
100 mM
DTT [º°Ã·]
0.1%
BSA [º°Ã·]
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Escherichia coli carrying the plasmid containing phage SP6 RNA polymerase gene
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37¡É, pH7.5ÀÇ Á¶°Ç¿¡¼ 1½Ã°£ µ¿¾È 1 nmolÀÇ [
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40 mM
Tris-HCl (pH7.5)
6 mM
MgCl
2
10 mM
DTT
2 mM
Spermidine
°¢ 0.5 mM
ATP, UTP, CTP
0.5 mM
[
3
H]GTP
0.01%
BSA
1 §¶/100 §¡
pSP65 DNA
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- 500 UÀÇ º» È¿¼Ò¿Í 1 §¶ÀÇ ¥ëDNA-Hind III ºÐÇØ¹°À» 37¡É, 16½Ã°£ ¹ÝÀÀÇÏ¿©µµ DNAÀÇ Àü±â¿µµ¿ ÆÐÅÏ¿¡ º¯È°¡ ¾ø´Ù. - 500 UÀÇ º» È¿¼Ò¿Í 1 §¶ÀÇ 16S¿Í 23S rRNA¸¦ 37¡É, 16½Ã°£ ¹ÝÀÀÇÏ¿©µµ RNAÀÇ Àü±â¿µµ¿ ÆÐÅÏ¿¡ º¯È°¡ ¾ø´Ù. - 500 UÀÇ º» È¿¼Ò¿Í 1 §¶ÀÇ closed circular (RF I) pBR322 DNA¸¦ 37¡É, 4½Ã°£ ¹ÝÀÀÇÏ¿©µµ nicking Ȱ¼ºÀÌ ¾ø´Ù. - º» È¿¼Ò´Â SDS Àü±â¿µµ¿¿¡ ÀÖ¾î¼, 95% ÀÌ»óÀÇ ¼øµµ¸¦ ³ªÅ¸³½´Ù.
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- »ç¿ë½Ã´Â ÷ºÎ ¹öÆÛ¿¡ ¹Ýµå½Ã DTT¿Í BSA¸¦ ÷°¡ÇÒ °Í. À̵éÀ» ÇÔÀ¯ÇÏÁö ¾ÊÀ¸¸é Ȱ¼ºÈµÇÁö ¾Ê´Â °æ¿ì°¡ ÀÖ´Ù.
- ÃÖÀû¿Âµµ´Â 40¡É(37¡ÉÀÇ ¾à 130%)·Î, È¿¼Ò·®Àº 300 U/ml±îÁö, ÁÖÇü·®(template DNA)Àº 100 §¶/ml ±îÁö´Â ÇÕ¼º·®¿¡ ´ëÇØ (+)»ó°ü °ü°è¸¦ ³ªÅ¸³½´Ù.
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- ƯÁ¤¿µ¿ª¸¸ÀÇ Àü»çÈ¿À²À» ³ôÀ̱â À§Çؼ´Â ±× ¿µ¿ªÀÇ ÇÏ·ù¿¡ ÁÖÇü DNA¸¦ Æò Ȱ ¶Ç´Â 5¡¯ µ¹Ã⸻´ÜÇüÀ¸·Î Àý´ÜÇÏ´Â °ÍÀÌ ÁÁ´Ù.
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- Northern hybridization°ú Southern hybridization¿ëÀÇ Ç¥½Ä RNA probeÀÇ Á¦ÀÛ
- RNA splicing ¹ÝÀÀÀÇ Àü±¸Ã¼ÀÇ Á¦ÀÛ
- Cap analog¸¦ ÇÁ¶óÀ̸ӷΠ»ç¿ëÇÑ capped mRNAÀÇ Á¦ÀÛ
Keyword :
¼ö½ÄÈ¿¼Ò£¯DNA Polymerase¡¢RNA Polymerase
Cloned
DNA Polymerase I (E. coli )
Cloned
Klenow Fragment (Large Fragment E. coli DNA Polymerase I)
Poly(A) Polymerase
Cloned
SP6 RNA Polymerase
Cloned
T4 DNA Polymerase
Cloned
T7 RNA Polymerase
Cloned
Terminal Deoxynucleotidyl Transferase