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Real Time PCR
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Cell direct qPCR
> qPCR/Western¿ë cell lysate Á¶Á¦½Ã¾à
°£´ÜÇÏ°Ô Á¶Á¦ÇÑ Lysate¿¡¼ mRNA¿Í ´Ü¹éÁúÀÇ ¹ßÇö Çؼ®
qPCR/Western¿ë cell lysate Á¶Á¦½Ã¾à
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Takara
3733
CellAmp¢â Direct Prep Kit for RT-PCR (Real Time) & Protein Analysis
200 ȸ
307,200¿ø
384,000¿ø
09.02 ~ 10.31
Product index
Overview
FASTest Real Time RT-P..
Dye-based Real Time PC..
Real Time PCR¿ë cDNA ..
One-step Real Time PCR..
Probe °ËÃâ¿ë Real Time..
Real Time PCR accessor..
The Best Tool for qPCR
qPCR Preamplification
PreAmp Master Mix
Real Time PCR ±â±â
Thermal Cycler Dice¢ç ..
Thermal Cycler Dice¢ç ..
CronoSTAR¢â 96 Real-Ti..
SmartChip¢â Real Time ..
cDNA ÇÕ¼º (Real Time PCR ..
gDNA Á¦°Å& RT ½Ã¾à (qP..
5x RT Master Mix (qPCR..
RT components (qPCR¿ë)
[New] ½Ã°£´ÜÃà, gDNA ..
»ùÇà dilution ½Ã¾à (qP..
EASY Dilution II (for ..
Intercalator TB Green °ËÃâ
Standard qPCR Mix
Long target¿ë qPCR Mix
Fast qPCR Mix
Dimer eraser qPCR Mix
GC rich qPCR Mix
Crude »ùÇÿë qPCR Mix
Premixed 1 step qRT-PC..
Specific 1 step qRT-PC..
Standard 1 step qRT-PC..
External Standard Kit ..
Transgene Detection Pr..
[New] Fast, UNG, dye p..
Dual-Labeled Probe °ËÃâ
Probe qPCR Mix MultiPl..
Probe qPCR Mix (¶Ç´Â U..
2 x all premix 1 step ..
Premix Ex Taq¢â (Probe..
[New] ½Ã°£´ÜÃà, gDNA ..
One Step PrimeScript¢â..
gDNA Á¦°Å& RT ½Ã¾à (qP..
5x RT Master Mix (qPCR..
RT components (qPCR¿ë)
PrimeDirect¢ç Probe RT..
Cell direct qPCR
RNA ÃßÃâ¾øÀÌ cell dire..
qPCR/Western¿ë cell ly..
1 step qRT-PCR¿ë cell ..
qPCR¿ë cell lysate Á¶..
Whole Transcriptome Áõ..
TB Green °ËÃâ(Clontech)
TB Green¢ç Advantage q..
Terra qPCR Direct TB G..
CycleavePCR °ËÃâ
Cycling Probe ¹ý¿¡ ´ë..
CycleavePCR Core Kit
CycleavePCR GBS Capsul..
CycleavePCR Streptococ..
CycleavePCR¢â Reaction..
CycleavePCR¢â Salmonel..
º´¿øü¤ýÀ¯ÀüÀÚ °ËÃâ (Real ..
Bacteria (
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Provirus Copy Number D..
Transgene Detection Pr..
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CellAmp Washing Buffer
12.5 §¢¡¿2
CellAmp Processing Buffer
10 §¢
DNase I for Direct RNA Prep
200 §¡
5¡¿Loading Buffer
1 §¢¡¿2
1 M DTT (Dithiothreitol)
100 §¡¡¿2
* 96 well plate¿¡¼ ¹è¾çÇÑ ¼¼Æ÷ 200 well¿¡ ÇØ´çÇÏ´Â ¾çÀÌ´Ù.
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CellAmp Washing Buffer*, CellAmp Processing Buffer*, DNase I for Direct RNA Prep, 1 M DTT: -20 ¡É 5 ¡¿ Loading Buffer: °³ºÀ ÈÄ ½Ç¿Â ÀúÀå
* CellAmp Washing Buffer¿Í CellAmp Processing Buffer´Â ¿ëÇØ ÈÄ, 4¡É¿¡ º¸ Á¸ ÀúÀå °¡´ÉÇÏ´Ù. ÀÌ °æ¿ì, ¿À¿°¿¡ ÁÖÀÇÇÑ´Ù.
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º» Á¦Ç°Àº µ¿¹° ¹è¾ç¼¼Æ÷¿¡¼ Real Time RT-PCR·Î mRNA ¹ßÇöÇؼ®À» Çϰųª western blotÀ¸·Î ´Ü¹éÁú ¹ßÇö Çؼ®À» ÇÏ°íÀÚ ÇÒ ¶§ ¸ðµÎ ÀÌ¿ëÇÒ ¼ö ÀÖ´Â ¼¼Æ÷ ¿ëÇع°(lysate) Á¶Á¦¿ë Å°Æ®ÀÌ´Ù. °£´ÜÇÑ ÇÁ·ÎÅäÄÝ¿¡ ÀÇÇØ ¹è¾ç¼¼Æ÷·ÎºÎÅÍ ÃÖ´Ü 10ºÐ¸¸¿¡ lysateÀ» ÁغñÇÒ ¼ö ÀÖÀ¸¸ç, ÁغñÇÑ ¼¼Æ÷ lysate´Â º°µµÀÇ ÇÙ»ê Á¤Á¦³ª ´Ü¹éÁú ÃßÃâ°úÁ¤ ¾øÀÌ ±×´ë·Î ´ÙÄ«¶óÀÇ Real Time RT-PCR Å°Æ®¿¡ »ç¿ëÇÒ ÁÖÇü ¶Ç´Â ÀϹÝÀûÀÎ western blot Çؼ®¿ë »ùÇ÷ΠÀÌ¿ëÇÒ ¼ö ÀÖ´Ù. ÷ºÎµÈ SDS-PAGE ¿ë Loading Buffer´Â 2-Mercaptoethanol(2-ME)ÀÌ ÇÊ¿äÇÏÁö ¾Ê±â ¶§¹®¿¡ 2-ME free »óÅ¿¡¼ western blot ºÐ¼®À» ½Ç½ÃÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ º» Å°Æ®·Î Á¶Á¦ÇÑ lysate´Â One Step TB Green¢â PrimeScript RT-PCR Å°Æ® (Perfect Real Time) (TaKaRa Code RR066A/B)°ú ÇÔ²² Àü °úÁ¤ÀÌ ¾à 2½Ã°£ Á¤µµÀÇ ½Å¼Ó, °£ÆíÇÑ 1 step Real Time RT-PCR¿¡ »ç¿ëÇϰųª ¿ªÀü»ç ½Ã¾àÀÎ PrimeScript RT reagent Å°Æ® (Perfect Real Time) (TaKaRa Code RR037A/B)°ú ÇÔ²² Real Time PCR¿ë ÁÖÇü cDNA¸¦ Á¶Á¦ÇÏ´Â µî ´Ù¾çÇÑ Real Time RT-PCR °ü·Ã Á¦Ç°°ú ÇÔ²² ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.
º» Á¦Ç°°ú ÇÔ²² »ç¿ë °¡´ÉÇÑ Real Time RT - PCR °ü·Ã Á¦Ç°
1 step Real Time RT-PCR
TaKaRa Code
Á¦Ç°¸í
RR096A/B
One Step TB Green¢â PrimeScript PLUS RT-PCR Kit
(Perfect Real Time)
RR086A/B
One Step TB Green¢â PrimeScript RT-PCR Kit II
(Perfect Real Time)
RR066A/B
One Step TB Green¢â PrimeScript RT-PCR Kit
(Perfect Real Time)
RR064A/B
One Step PrimeScript RT-PCR Kit
(Perfect Real Time)
2 step Real Time RT-PCR
TaKaRa Code
Á¦Ç°¸í
RR037A/B
PrimeScript RT reagent Kit
(Perfect Real Time)
RR091A/B
TB Green¢â Premix DimerEraser (Perfect Real Time)
RR820A/B
TB Green¢â Premix Ex Taq II (Perfect Real Time)
RR420A/B
TB Green¢â Premix Ex Taq (Perfect Real Time)
RR390A/B
Premix Ex Taq (Perfect Real Time)
½ÇÇè flow
Keyword :
Cell direct qPCR
1.5½Ã°£ÀÌ¸é ¹è¾ç¼¼Æ÷¿¡¼ Real Time PCR °á°ú ..
RNA ÃßÃâ¾øÀÌ cell direct qPCR
°£´ÜÇÏ°Ô Á¶Á¦ÇÑ Lysate¿¡¼ mRNA¿Í ´Ü¹éÁúÀÇ ..
qPCR/Western¿ë cell lysate Á¶Á¦½Ã¾à
¹è¾ç¼¼Æ÷·ÎºÎÅÍ °£´ÜÇÏ°Ô 1 Step Real Time RT-..
1 step qRT-PCR¿ë cell lysate Á¶Á¦½Ã¾à
ºÒ°ú 10 ºÐ ¾È¿¡ ¹è¾ç ¼¼Æ÷·ÎºÎÅÍ Real Time RT..
qPCR¿ë cell lysate Á¶Á¦½Ã¾à
1 °³ÀÇ ¼¼Æ÷·ÎºÎÅÍ template cDNA¸¦ ÁõÆø °¡´É
Whole Transcriptome ÁõÆø½Ã¾à