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Home > ÀüÁ¦Ç°º¸±â > Real Time PCR > Cell direct qPCR > qPCR/Western¿ë cell lysate Á¶Á¦½Ã¾à
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qPCR/Western¿ë cell lysate Á¶Á¦½Ã¾à

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Takara
3733
CellAmp¢â Direct Prep Kit for RT-PCR (Real Time) & Protein Analysis
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200 ȸ
307,200¿ø 
384,000¿ø
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09.02 ~ 10.31
3733_e.v2201Da.pdf

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CellAmp Washing Buffer 12.5 §¢¡¿2
CellAmp Processing Buffer 10 §¢
DNase I for Direct RNA Prep 200 §¡
5¡¿Loading Buffer 1 §¢¡¿2
1 M DTT (Dithiothreitol) 100 §¡¡¿2
* 96 well plate¿¡¼­ ¹è¾çÇÑ ¼¼Æ÷ 200 well¿¡ ÇØ´çÇÏ´Â ¾çÀÌ´Ù.
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CellAmp Washing Buffer*, CellAmp Processing Buffer*, DNase I for Direct RNA Prep, 1 M DTT: -20 ¡É 5 ¡¿ Loading Buffer: °³ºÀ ÈÄ ½Ç¿Â ÀúÀå
* CellAmp Washing Buffer¿Í CellAmp Processing Buffer´Â ¿ëÇØ ÈÄ, 4¡É¿¡ º¸ Á¸ ÀúÀå °¡´ÉÇÏ´Ù. ÀÌ °æ¿ì, ¿À¿°¿¡ ÁÖÀÇÇÑ´Ù.
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º» Á¦Ç°Àº µ¿¹° ¹è¾ç¼¼Æ÷¿¡¼­ Real Time RT-PCR·Î mRNA ¹ßÇöÇؼ®À» Çϰųª western blotÀ¸·Î ´Ü¹éÁú ¹ßÇö Çؼ®À» ÇÏ°íÀÚ ÇÒ ¶§ ¸ðµÎ ÀÌ¿ëÇÒ ¼ö ÀÖ´Â ¼¼Æ÷ ¿ëÇع°(lysate) Á¶Á¦¿ë Å°Æ®ÀÌ´Ù. °£´ÜÇÑ ÇÁ·ÎÅäÄÝ¿¡ ÀÇÇØ ¹è¾ç¼¼Æ÷·ÎºÎÅÍ ÃÖ´Ü 10ºÐ¸¸¿¡ lysateÀ» ÁغñÇÒ ¼ö ÀÖÀ¸¸ç, ÁغñÇÑ ¼¼Æ÷ lysate´Â º°µµÀÇ ÇÙ»ê Á¤Á¦³ª ´Ü¹éÁú ÃßÃâ°úÁ¤ ¾øÀÌ ±×´ë·Î ´ÙÄ«¶óÀÇ Real Time RT-PCR Å°Æ®¿¡ »ç¿ëÇÒ ÁÖÇü ¶Ç´Â ÀϹÝÀûÀÎ western blot Çؼ®¿ë »ùÇ÷ΠÀÌ¿ëÇÒ ¼ö ÀÖ´Ù. ÷ºÎµÈ SDS-PAGE ¿ë Loading Buffer´Â 2-Mercaptoethanol(2-ME)ÀÌ ÇÊ¿äÇÏÁö ¾Ê±â ¶§¹®¿¡ 2-ME free »óÅ¿¡¼­ western blot ºÐ¼®À» ½Ç½ÃÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ º» Å°Æ®·Î Á¶Á¦ÇÑ lysate´Â One Step TB Green¢â PrimeScript RT-PCR Å°Æ® (Perfect Real Time) (TaKaRa Code RR066A/B)°ú ÇÔ²² Àü °úÁ¤ÀÌ ¾à 2½Ã°£ Á¤µµÀÇ ½Å¼Ó, °£ÆíÇÑ 1 step Real Time RT-PCR¿¡ »ç¿ëÇϰųª ¿ªÀü»ç ½Ã¾àÀÎ PrimeScript RT reagent Å°Æ® (Perfect Real Time) (TaKaRa Code RR037A/B)°ú ÇÔ²² Real Time PCR¿ë ÁÖÇü cDNA¸¦ Á¶Á¦ÇÏ´Â µî ´Ù¾çÇÑ Real Time RT-PCR °ü·Ã Á¦Ç°°ú ÇÔ²² ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.

º» Á¦Ç°°ú ÇÔ²² »ç¿ë °¡´ÉÇÑ Real Time RT - PCR °ü·Ã Á¦Ç°
1 step Real Time RT-PCR
TaKaRa Code Á¦Ç°¸í
RR096A/B One Step TB Green¢â PrimeScript PLUS RT-PCR Kit
(Perfect Real Time)
RR086A/B One Step TB Green¢â PrimeScript RT-PCR Kit II
(Perfect Real Time)
RR066A/B One Step TB Green¢â PrimeScript RT-PCR Kit
(Perfect Real Time)
RR064A/B One Step PrimeScript RT-PCR Kit
(Perfect Real Time)
2 step Real Time RT-PCR
TaKaRa Code Á¦Ç°¸í
RR037A/B PrimeScript RT reagent Kit
(Perfect Real Time)
RR091A/B TB Green¢â Premix DimerEraser (Perfect Real Time)
RR820A/B TB Green¢â Premix Ex Taq II (Perfect Real Time)
RR420A/B TB Green¢â Premix Ex Taq (Perfect Real Time)
RR390A/B Premix Ex Taq (Perfect Real Time)
½ÇÇè flow

Keyword :

Cell direct qPCR
>
1.5½Ã°£ÀÌ¸é ¹è¾ç¼¼Æ÷¿¡¼­ Real Time PCR °á°ú ..
RNA ÃßÃâ¾øÀÌ cell direct qPCR
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°£´ÜÇÏ°Ô Á¶Á¦ÇÑ Lysate¿¡¼­ mRNA¿Í ´Ü¹éÁúÀÇ ..
qPCR/Western¿ë cell lysate Á¶Á¦½Ã¾à
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¹è¾ç¼¼Æ÷·ÎºÎÅÍ °£´ÜÇÏ°Ô 1 Step Real Time RT-..
1 step qRT-PCR¿ë cell lysate Á¶Á¦½Ã¾à
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ºÒ°ú 10 ºÐ ¾È¿¡ ¹è¾ç ¼¼Æ÷·ÎºÎÅÍ Real Time RT..
qPCR¿ë cell lysate Á¶Á¦½Ã¾à
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1 °³ÀÇ ¼¼Æ÷·ÎºÎÅÍ template cDNA¸¦ ÁõÆø °¡´É
Whole Transcriptome ÁõÆø½Ã¾à