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Home > ÀüÁ¦Ç°º¸±â > Real Time PCR > Dual-Labeled Probe °ËÃâ > PrimeDirect¢ç Probe RT-qPCR Mix (¶Ç´Â UNG Æ÷ÇÔ)
Ç÷¾×, ¼Òº¯ µî »ýü»ùÇÃÀÇ direct qRT-PCR

PrimeDirect¢ç Probe RT-qPCR Mix (¶Ç´Â UNG Æ÷ÇÔ)

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
RR650A
PrimeDirect¢ç Probe RT-qPCR Mix
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
200 ȸ
(25 §¡ ¹ÝÀÀ°è)
516,000¿ø  RR650A_e.v2207Da.pdf
Takara
RR650B
PrimeDirect¢ç Probe RT-qPCR Mix
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1,000 ȸ (RR650A x 5)
2,320,000¿ø 
Takara
RR651A
PrimeDirect¢ç Probe RT-qPCR Mix, with UNG
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
200 ȸ
(25 §¡ ¹ÝÀÀ°è)
540,000¿ø  RR651A_e.v2207Da.pdf
Takara
RR651B
PrimeDirect¢ç Probe RT-qPCR Mix, with UNG
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1,000 ȸ (RR651A x 5)
2,430,000¿ø 


  • 2 x premix ŸÀÔÀÇ 1 step Real Time RT-PCR ½Ã¾à
  • Ç÷¾×, ¼Òº¯ µîÀ¸·ÎºÎÅÍ RNA Á¤Á¦¾øÀÌ Real Time RT-PCR °¡´É
  • Carryover contamination ¹æÁö¸¦ À§ÇÑ UNG plus ¹öÀü ¼±Åà °¡´É (RR651A/B)
Á¦Ç°¼³¸í
PrimeDirect¢ç Probe RT-qPCR Mix´Â Dual-Labeled Probe¹ý (5'-nuclease method)ÀÇ 1 step Real Time RT-PCR ½Ã¾àÀÌ´Ù. 2 x premix ½Ã¾àÀ¸·Î Ÿ°Ù °ËÃâÀ» À§ÇÑ primer, probe¿Í template¸¸ ÷°¡ÇÏ¸é °£´ÜÈ÷ ¹ÝÀÀÀ» ÁøÇàÇÒ ¼ö ÀÖÀ» »Ó¸¸ ¾Æ´Ï¶ó, º°µµÀÇ ÇÙ»ê Á¤Á¦°úÁ¤ ¾øÀÌ RNA virus µîÀ» Æ÷ÇÔÇÏ´Â »ýü½Ã·á »ùÇÃÀ» ¹ÝÀÀ¾×¿¡ Á÷Á¢ ³Ö¾î Real Time RT-PCRÀ» ÁøÇàÇÒ ¼ö ÀÖ´Ù.
ÇϳªÀÇ tube¿¡¼­ ÇÙ»ê ÃßÃâ, ¿ªÀü»ç ¹ÝÀÀ, Real Time PCRÀ» ¿¬¼ÓÀûÀ¸·Î ½Ç½ÃÇϹǷΠ¸Å¿ì Æí¸®Çϸç, ¹ÝÀÀ½Ã°£ ¶ÇÇÑ Å©°Ô ÁÙÀÏ ¼ö ÀÖ´Ù.
¶ÇÇÑ °Ë»ç µîÀÇ ¸ñÀûÀ¸·Î ƯÁ¤ Ÿ°ÙÀ» ¹Ýº¹Çؼ­ °ËÃâÇÏ´Â ½ÇÇè½Ç¿¡¼­ ÈçÈ÷ ¹ß»ýÇÒ ¼ö ÀÖ´Â carryover contaminationÀ» ¹æÁöÇϱâ À§ÇØ Uracil-N-Glycosylase (UNG)°¡ Æ÷ÇÔµÈ PrimeDirect¢ç Probe RT-qPCR Mix, with UNG (Code RR651A/B)¸¦ »ç¿ëÇÒ ¼ö ÀÖ´Ù. Premix ¾È¿¡ Æ÷ÇÔµÈ UNG´Â dUTP¸¦ Æ÷ÇÔÇÑ PCR »ê¹°À» ºÐÇØÇÒ ¼ö ÀÖÀ¸¹Ç·Î, carryover contamination¿¡ ÀÇÇÑ false positive ÁõÆøÀ» ÇÇÇÒ ¼ö ÀÖ´Ù*.
º» Á¦Ç°Àº Heparin (blood)À̳ª humic acid (Åä¾ç) µî ´Ù¾çÇÑ PCR inhibitor¿¡ ´ëÇØ ÃÖ°í ¼öÁØÀÇ ³»¼º°ú ³ôÀº ÁõÆøÈ¿À²À» º¸À̹ǷÎ, ´Ù¾çÇÑ »ùÇÿ¡¼­ virus³ª ¼¼±ÕÀÇ direct °ËÃâ, À¯ÀüÀÚ ¹ßÇö Çؼ® µî¿¡ »ç¿ëÇÒ ¼ö ÀÖ´Ù.

(*PrimeDirect¢ç Probe RT-qPCR Mix, with UNG »ç¿ë ½Ã)
³»¿ë
PrimeDirect¢ç Probe RT-qPCR Mix (Code RR650A, 25 §¡ ¹ÝÀÀ ½Ã 200ȸ ºÐ·®)
1.

PrimeDirect Probe RT-qPCR Mix (2¡¿)

625 §¡¡¿ 4

2.

RNase Free Water

1.25 §¢¡¿ 2

3.

ROX Reference Dye (50¡¿ conc.)*

100 §¡

4.

ROX Reference Dye II (50¡¿ conc.)*

100 §¡


PrimeDirect¢ç Probe RT-qPCR Mix, with UNG (Code RR651A, 25 §¡ ¹ÝÀÀ ½Ã 200ȸ ºÐ·®)
1.

PrimeDirect Probe RT-qPCR Mix, with UNG (2¡¿)

625 §¡¡¿ 4

2.

RNase Free Water

1.25 §¢¡¿ 2

3.

ROX Reference Dye (50¡¿ conc.)*

100 §¡

4.

ROX Reference Dye II (50¡¿ conc.)*

100 §¡


* Real Time PCR ÀåÄ¡ Áß well °£ Çü±¤ ½ÅÈ£¸¦ º¸Á¤ÀÌ ÇÊ¿äÇÑ ÀåÄ¡·Î Çؼ®ÇÏ´Â °æ¿ì¿¡ »ç¿ëÇÑ´Ù.
  • ROX Reference Dye (50¡¿)¸¦ ÷°¡ÇÏ´Â ±âÁ¾
    StepOnePlus Real-Time PCR System (Thermo Fisher Scientific) ¿Ü
  • ROX Reference Dye II (50¡¿)¸¦ ÷°¡ÇÏ´Â ±âÁ¾
    Applied Biosystems 7500 Fast Real-Time PCR System (Thermo Fisher Scientific) ¿Ü
  • ÷°¡°¡ ÇÊ¿ä ¾ø´Â ±âÁ¾
    CronoSTAR¢â 96 Real-Time PCR System (4ch/6ch)
    Thermal Cycler Dice Real Time System III (Code TP950/TP970/TP980)
    LightCycler 96/LightCycler 480 System (Roche Diagnostics)
    CFX96 Real-Time PCR Detection System (Bio-Rad)
º¸Á¸ -20¡É
Ư¡
1. Probe °ËÃâ¿ë 1 step Real Time RT PCR ½Ã¾à
2. ¹ÝÀÀ¾×¿¡ Á÷Á¢ »ýü½Ã·á¸¦ ³Ö¾î RNA ¿­ÃßÃâ ÈÄ Real Time RT-PCR ÁøÇà
3. °í¿Â¿¡¼­ ¿ªÀü»ç ¹ÝÀÀ (55~65¡É)
4. ´Ù¾çÇÑ ÀúÇع°Áú¿¡ ´ëÇÑ ÃÖ°í ¼öÁØÀÇ ³»¼º
5. UNG°¡ Æ÷ÇÔµÈ premix·Î carryover contamination ¹æÁö (Code RR651A/B¿¡ ÇØ´ç)


±×¸². RNA ÃßÃâ°úÁ¤ ¾ø´Â °£´ÜÇÑ ½ÇÇè workflow
¹ÝÀÀ¾ïÁ¦¹°Áú¿¡ ´ëÇÑ ³»¼º½ÇÇè ¿¹
[´Ù¾çÇÑ ¹ÝÀÀÀúÇع°ÁúÀÌ Á¸ÀçÇÏ´Â »ùÇÿ¡¼­ÀÇ ¹ÝÀÀ¼º ºñ±³]
- Template: Human HeLa total RNA 1 ng
- Target: Human ACTB À¯ÀüÀÚ
- °á°ú: °¢ Á¦Ç°ÀÇ ÇÁ·ÎÅäÄÝ¿¡ µû¶ó PrimeDirect¢ç ¹× Ÿ»ç Á¦Ç°ÀÇ 1 step Real Time RT-PCR ½Ã¾àÀ» ÀÌ¿ëÇÏ¿© ´Ù¾çÇÑ ¾ïÁ¦Á¦°¡ ÷°¡µÈ »ùÇÃÀ» ¹ÝÀÀÇÑ °á°ú, PrimeDirect¢ç Á¦Ç°ÀÌ °¢Á¾ ÀúÇع°Áú¿¡ ´ëÇØ °¡Àå ³ôÀº ³»¼ºÀ» °¡Áö´Â °ÍÀ» È®ÀÎÇÏ¿´´Ù.


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