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Home > ÀüÁ¦Ç°º¸±â > Real Time PCR > Dual-Labeled Probe °ËÃâ > Premix Ex Taq¢â (Probe qPCR)

Premix Ex Taq¢â (Probe qPCR)

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
RR390A
Premix Ex Taq¢â (Probe qPCR)
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
200 ȸ
(50 §¡ ¹ÝÀÀ½Ã)
254,400¿ø 
318,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR390A_e.v2201Da.pdf
Takara
RR390B
Premix Ex Taq¢â (Probe qPCR)
°ü·ÃÇмú ±¸¸ÅÇϱâ
400 ȸ (RR390A x 2)
572,000¿ø 
Takara
RR390L
Premix Ex Taq¢â (Probe qPCR), Bulk
°ü·ÃÇмú ±¸¸ÅÇϱâ
200 ȸ
(50 §¡ ¹ÝÀÀ½Ã)
254,400¿ø 
318,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR390L_e.v2201Da.pdf
Takara
RR390LB
Premix Ex Taq¢â (Probe qPCR), Bulk
°ü·ÃÇмú ±¸¸ÅÇϱâ
400 ȸ (RR390LA x 2)
572,000¿ø 
Takara
RR39LR
Premix Ex Taq¢â (Probe qPCR), ROX Plus
°ü·ÃÇмú ±¸¸ÅÇϱâ
200 ȸ
(50 §¡ ¹ÝÀÀ½Ã)
254,400¿ø 
318,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR39LR_e.v2201Da.pdf
Takara
RR39LRB
Premix Ex Taq¢â (Probe qPCR), ROX Plus
°ü·ÃÇмú ±¸¸ÅÇϱâ
400 ȸ (RR39LRA x 2)
572,000¿ø 



º» Á¦Ç°Àº RR039A Premix Ex Taq (Perfect Real Time)ÀÇ ´ëüǰ ÀÔ´Ï´Ù.

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  • ´Ù¾çÇÑ Real Time PCR±â±â¿¡ ³ôÀº ƯÀ̼º ³ªÅ¸³¿
  • TaKaRa Ex Taq¢â HS ä¿ë - °í°¨µµ, °íÈ¿À²
  • 2¡¿ premix¿¡ ³»¿­¼º RNaseH ÷°¡ - ÀÜÁ¸ mRNA¿¡ ÀÇÇÑ PCR ÀúÇعÝÀÀ ¾ïÁ¦
  • ±âº» ÇÁ·ÎÅäÄÝ°ú °í¼Ó ÇÁ·ÎÅäÄÝ¿¡ ¸ðµÎ »ç¿ë°¡´É
Á¦Ç°¼³¸í
Premix Ex Taq¢â (Probe qPCR)Àº Dual-Labeled Probe ¸¦ ÀÌ¿ëÇÑ Real Time PCR Àü¿ë ½Ã¾àÀÌ´Ù.
Anti-Taq Ç×ü¸¦ ÀÌ¿ëÇÑ hot start PCR È¿¼ÒÀÎ TaKaRa Ex Taq HS¿Í Real Time PCR¿ëÀ¸·Î ÃÖÀûÈ­ ½ÃŲ buffer¸¦ ÀÌ¿ëÇÏ¿© ºñƯÀÌÀû ÁõÆøÀ» ¾ïÁ¦ÇÒ ¼ö ÀÖ¾î ³ôÀº ÁõÆø È¿À², ³ôÀº °ËÃâ °¨µµÀÇ ÁõÆøÀ» ½ÇÇöÇÏ°í ÀÖ´Ù.
2¡¿ ³óµµÀÇ premix type½Ã¾àÀ¸·Î ¹ÝÀÀ¾× Á¶Á¦°¡ °£´ÜÇÏ°í, ³»¿­¼º RNaseHÀÎ Tli RNaseH °¡ 2¡¿ Premix ½Ã¾à Áß¿¡ ÷°¡µÇ¾î ÀÖ¾î cDNA¸¦ ÁÖÇüÀ¸·Î ÇÑ °æ¿ì ÀÜÁ¸ mRN¿¡ ÀÇÇÑ PCR ¹ÝÀÀ ÀúÇظ¦ ±ØÇÑÀ¸·Î ¾ïÁ¦ ÇÒ ¼ö ÀÖ´Ù.
¶Ç °í¼Ó ÇÁ·ÎÅäÄݵµ »ç¿ëÇÒ ¼ö ÀÖÀ¸¸ç, Æø³ÐÀº ¹üÀ§¿¡¼­ º¸´Ù Á¤È®ÇÑ Å¸°ÙÀÇ Á¤·® ¹× °ËÃâÀ» ÀçÇö¼ºÀÖ°Ô ÁøÇàÇÒ ¼ö ÀÖ´Ù.

Real Time RT-PCR½Ã ¿ªÀü»ç¹ÝÀÀÀº ¾Æ·¡ÀÇ Á¦Ç°À» ÃßõÇÕ´Ï´Ù.
  • PrimeScript¢ç RT reagent Kit with gDNA Eraser (Perfect Real Time) (Code RR047A/B),
  • PrimeScript¢ç RT Master Mix (Perfect Real Time) (Code RR036A/B)
  • PrimeScript¢ç RT reagent Kit (Perfect Real Time) (Code RR037A/B)
³»¿ë

Premix Ex Taq¢â(Probe qPCR) (Code RR390A, 50 §¡ ¹ÝÀÀ½Ã 200ȸ ºÐ·®)
Premix Ex Taq (Probe qPCR) (2¡¿conc.)*1

1 §¢¡¿5

ROX Reference Dye (50¡¿conc.)*2

200 §¡

ROX Reference Dye II (50¡¿conc.)*2

200 §¡


Premix Ex Taq¢â (Probe qPCR), Bulk (Code RR390L, 50 §¡ ¹ÝÀÀ½Ã 200ȸ ºÐ·®)
Premix Ex Taq (2¡¿) (Probe qPCR) , Bulk*1

5 §¢

ROX Reference Dye (50¡¿conc.)*3

200 §¡


Premix Ex Taq¢â (Probe qPCR), ROX plus (Code RR39LR, 50 §¡ ¹ÝÀÀ½Ã 200ȸ ºÐ·®)
Premix Ex Taq (2¡¿) (Probe qPCR), ROX plus*4

5 §¢


*1 TaKaRa Ex Taq HS, dNTP Mixture, Mg2+, Tli RNaseHÆ÷ÇÔ

*2 Life TechnologiesÀÇ Real Time PCR ÀåÄ¡ µî well °£ Çü±¤ ½ÅÈ£¸¦ º¸Á¤ÇÏ´Â ÀåÄ¡·Î Çؼ®ÇÏ´Â °æ¿ì¿¡ »ç¿ëÇÑ´Ù.
  • ROX Reference Dye(50¡¿)À» ÷°¡ÇÏ´Â ±âÁ¾
    Applied Biosystems 7300 Real-Time PCR System (Life Technologies)
    StepOnePlus Real-Time PCR System (Life Technologies)
  • ROX Reference Dye II(50¡¿)À» ÷°¡ÇÏ´Â ±âÁ¾
    Applied Biosystems 7500/7500 Fast Real-Time PCR System (Life Technologies)
  • ÷°¡°¡ ÇÊ¿ä ¾ø´Â ±âÁ¾
    CronoSTAR¢â 96 Real-Time PCR System (4ch) (Code 640231)
    Thermal Cycler Dice Real Time System III (Code TP950/TP970/TP980)
    Thermal Cycler Dice Real Time System II (Code TP900/TP960)
    Thermal Cycler Dice Real Time System Single (Code TP850/TP870)
    Thermal Cycler Dice Real Time System Lite (Code TP700/TP760)
    Smart Cycler System/Smart Cycler II System (Cepheidȍ)
    LightCycler 96/LightCycler 480 System (Roche Diagnosticsȍ)
    CFX96 Real-Time PCR Detection System (Bio-Radȍ)

º¸Á¸
4 ¡É º¸Á¸: 6°³¿ù°£ ¾ÈÁ¤
(Freezing & thawing ¹Ýº¹À» ÇÇÇØÁÖ¼¼¿ä.)

- Àå±â º¸Á¸ÀÇ °æ¿ì´Â -20 ¡É¿¡¼­ º¸°üÇϽʽÿÀ.
- ÀÏ´Ü ¿ëÇØ ÈÄ¿¡´Â 4¡É º¸°üÇÏ°í, 6°³¿ù¾È¿¡ »ç¿ëÇÒ °ÍÀ» ±ÇÀåÇÕ´Ï´Ù.
»ç¿ë»ó ÁÖÀÇ»çÇ×
1. »ç¿ë½Ã¿¡´Â °ÅÇ°ÀÌ »ý±âÁö ¾Ê°Ô ºÎµå·´°Ô ³ì¿©ÁØ ÈÄ, ½Ã¾àÀ» ¿ÏÀüÈ÷ ¼¯¾î¼­ »ç¿ëÇØ ÁֽʽÿÀ. ½Ã¾àÀÌ ¿ÏÀüÈ÷ ³ìÁö¾ÊÀº °æ¿ì È¿À²ÀÌ ÀúÇ쵃 ¼ö ÀÖ½À´Ï´Ù.
    Vortex´Â »ç¿ëÇÏÁö ¸»¾Æ ÁֽʽÿÀ. Premix Ex Taq¢â (Probe qPCR) (2¡¿conc.)À» -20 ¡É¿¡¼­ º¸°üÇßÀ» °æ¿ì, º¸°ü Áß¿¡ ħÀüÀÌ »ý±â´Â °æ¿ì°¡ ÀÖ½À´Ï´Ù.
    °¡º±°Ô ¼ÕÀ¸·Î ³ìÀÌ°í ½Ç¿Â¿¡ µÎ½Ã¸é, ¿ÏÀüÈ÷ ¿ëÇص˴ϴÙ. ¹Ýµå½Ã ¿ÏÀüÇÏ°Ô ¼¯Àº ÈÄ »ç¿ëÇØ ÁֽʽÿÀ.

2. ³ìÀÎ ½Ã¾àÀº ¹Ýµå½Ã ¾óÀ½¿¡ ²È¾Æ ÁֽʽÿÀ.

3. º» Á¦Ç°Àº Dual-Labeled probe¸¦ Æ÷ÇÔÇÏ°í ÀÖÁö ¾Ê½À´Ï´Ù. °ËÃâÇÏ°íÀÚ ÇÏ´Â probe¸¦ º°µµ·Î ÁغñÇØ ÁֽʽÿÀ.

Keyword : RR039A,RR039B,RR039W

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