* CHOgro^® Expression Media, Dry Powder, Prepares 10 Liters와 CHOgro^® Expression Media, 10 Liter Polybag는 최소 주문수량이 있는 custom order 제품(주문 후 생산)으로, 주문 전 반드시 다카라코리아 고객지원 (02-2081-2510)으로 문의바랍니다.

제품 설명

CHOgro^® Expression System은 suspension CHO Cell을 이용하여 대량의 단백질 발현에 최적인 제품이다. Transfection 시약과 배지가 모두 포함된 All-in-one kit이며, 다른 culture system을 사용하고 있는 연구자도 CHOgro Expression Medium 에 24시간 적응 후 Transfection을 진행하면, 간편하게 높은 단백질 발현을 경험할 수 있다.

• 셋업이 간편: 대량 단백질 생산에 최적인 프로토콜 제공
• Cell clumping을 최소화하여, 높은 밀도의 CHO cell 배양 (12x10^6 cells/ml) 및 뛰어난 viability 실현
• Chemically defined, hydrolysate-free, animal origin free medium
• cGMP (국제의약품 품질 기준) 조건에서 생산 - CHOgro^® Expression Medium

CHOgro^® Expression System 구성

구성품	용량	별도구매 가능
TransIT-PRO® Transfection Reagent	1 ml	MIR 5740
CHOgro® Expression Medium	2 × 1000 ml	MIR 6200
CHOgro® Complex Formation Solution	100 ml	MIR 6210
Poloxamer 188 Solution	100 ml	MIR 6230
L-Glutamine Solution	100 ml	MIR 6240

Media 종류에 따른 세포 배양 밀도 및 viability 비교

Figure 1. Suspension CHO cells grow to high density in the CHOgro^® Expression Medium. Tripli-cate flasks of FreeStyle™ CHO-S cells were seeded in CHOgro^® Expression Medium (red line) or FreeStyle™ CHO Expression Medium (blue line) at a cell density of 0.5 x 10⁶cells/㎖. Cell counts (solid line) and viability (dotted line) were measured daily using a Guava easyCyte™ 5HT flow cytometer.

Expression system에 따른 단백질 발현 비교

Figure 2. CHOgro^® Expression Medium yields multi-fold increases in antibody titer. Human IgG1 was produced by transient transfection using TransIT-PRO^® (1:1), FreeStyle™ MAX (1.25:1.25) or 25 kDa linear PEI (6:1) transfection reagents according to the manufacturers’ or published protocol (reagent:DNA ratio). FreeStyle™ CHO-S cells were transfected using 1㎍ plasmid DNA per milliliter of culture and cell densities of 2 x 10⁶ cells/㎖ or 1 x 10⁶ cells/㎖ for the CHOgro^® Expression Medium (red bars) or FreeStyle™ Expression Medium (blue bars). Antibody levels were also analyzed from day 6 clarified supernatants using a human IgG ELISA.

간편한 culture system 변경과 발현율 증가

Figure 3. Media exchange leads to higher protein production. Free-Style™ CHO-S cells were cultured in FreeStyle™ CHO Expression Medium or CHOgro^® Expression Medium and 24 hours prior to transfection a subset of the cells grown in FreeStyle™ CHO Expression Medium were spun down and exchanged with 100% fresh CHOgro^® Expression Medium. The cells were allowed to grow and adapt for 24 hours prior to transfection with FreeStyle™ MAX or TransIT-PRO^® transfection reagents according to the manufacturers’ protocol and a hIgG1 encoding construct. (A) Workflow schematic of media exchange of CHO-S cells from FreeStyle™ CHO Expression Medium to CHOgro^® Expression Medium (black arrow) or the normal CHOgro^® Expression System (red arrow) (B) Day 6 supernatants were clarified and analyzed using a human IgG ELISA. Data is normalized to the complete CHOgro^® Expression System (red bar).

대용량 (10L) CHOgro^® Expression Media

그림 4. 대량의 CHO cell 배양을 위한 대용량의 CHOgro^® Expression Media. Powder 형태 (좌), Polybag 형태 (우)

Human IgG1 Expression Control

포유류 세포에서 항체 발현 확인용 control

Figure 5. Human IgG1 Expression Control is an Optimized Premix of Heavy and Light Chain Constructs. The Human IgG1 Expression Control is an equimolar premix of proprietary human IgG1 heavy and light chain expression constructs with antibody expression driven by CMV promoters. This product incorporates DNA2.0 IP-Freeⓒ vector technology.

관련 제품

고발현 transfection reagent TransIT-PRO^® Transfection Reagent 정보 자세히 보기
Endotoxin 제거를 통한 단백질 발현 향상 MiraCLEAN^® Endotoxin Removal Kit 자세히보기
Transfection 효율 확인용 Label IT^® Plasmid Delivery Control, Cy^® 3 자세히보기