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Home > ÀüÁ¦Ç°º¸±â > Gene transfer (virus) > Mammalian Cell ¿ë Vector > pIRES Bicistronic Expression Vector

pIRES Bicistronic Expression Vector

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
631605
pIRES Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
807,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33396
Clontech
631622
pIRESbleo3 Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
807,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33402
Clontech
631620
pIREShyg3 Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
807,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33403
Clontech
631621
pIRESneo3 Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
807,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33404
Clontech
631619
pIRESpuro3 Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
807,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33405
Clontech
632435
pIRES2-AcGFP1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
1,116,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33398
Clontech
632515
pIRES2-AcGFP1-Nuc Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
1,116,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33399
Clontech
632420
pIRES2-DsRed2 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
1,116,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33400
Clontech
632540
pIRES2 DsRed-Express2 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
1,082,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33397
Clontech
632478
pIRES2-ZsGreen1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
1,116,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33401
Clontech
632520
pRetroX-IRES-ZsGreen1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
1,076,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33539, x33005
Clontech
632521
pRetroX-IRES-DsRed-Express Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
1,076,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33538, x33005
Clontech
635003
pEXP-Lib Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
679,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33363

  • ¸ñÀû ´Ü¹éÁúÀ» °í¹ßÇöÇÏ´Â ¼¼Æ÷¸¦ Ç×»ýÁ¦³ª Çü±¤À» ÀÌ¿ëÇØ ºü¸£°Ô ¼±º°
  • Bicistronic expressionÀ¸·Î stable cloneÀ» ºü¸£°í, Á¤È®ÇÏ°Ô ¼±º°
pIRES Bicistronic Expression Vector´Â ¸ñÀû ´Ü¹éÁúÀ» ¹ßÇöÇÏ´Â cloneÀ» ºü¸£°í È¿°úÀûÀ¸·Î ¼±º°ÇÒ ¼ö ÀÖ°Ô ÇÑ´Ù. ÀÌ vector´Â ÇϳªÀÇ promoter·Î ¸ñÀû À¯ÀüÀÚ¿Í ¼±º° ¸¶Ä¿ (Figure 1) ¶Ç´Â Living Colors Fluorescent Protein (Figure 2) ¸ðµÎ¸¦ ¹ßÇöÇÒ ¼ö ÀÖ´Â cassette¸¦ °¡Áö°í ÀÖ´Ù. ±×·¯¹Ç·Î ½ÇÁúÀûÀ¸·Î ¸¶Ä¿¸¦ ¹ßÇöÇÏ´Â ¼¼Æ÷´Â ¸ñÀû À¯ÀüÀÚ¸¦ ¹ßÇöÇÑ´Ù. ÀÌ·± vector¸¦ ÀÌ¿ëÇϸé ÃÖ¼ÒÇÑÀÇ ¼±º°°úÁ¤À¸·Î ¸ñÀû ´Ü¹éÁúÀ» °í¹ßÇöÇÏ´Â cloneÀ» ¼±º°ÇÒ¼ö ÀÖ´Ù.
IRES Bicistronic Expression
³ú½É±Ù¿°¹ÙÀÌ·¯½º (encephalomyocarditis virus (ECMV))ÀÇ IRES (internal ribosome entry site)´Â ÇϳªÀÇ mRNA·ÎºÎÅÍ 2 °³ÀÇ ORF (open reading frame)¸¦ ¹ø¿ªÇϵµ·Ï ÇÑ´Ù (1, 2). RibosomeÀº bicistronic mRNAÀÇ 5£§-¸»´Ü¿¡¼­ºÎÅÍ ¸ñÀû À¯ÀüÀÚ¸¦ ¹ø¿ªÇϸç, ¼±º° ¸¶Ä¿ ¶Ç´Â Çü±¤ reporter¸¦ ¹ø¿ªÇϱâ À§ÇÏ¿© IRES¿¡ °áÇÕÇÑ´Ù. ¸ðµç pIRES Antibiotic Selection Vector´Â cloningµÈ À¯ÀüÀÚÀÇ ¹ø¿ª °³½Ã È¿À²¿¡ ºñÇÏ¿© »ó´ëÀûÀ¸·Î ¼±º° ¸¶Ä¿ÀÇ ¹ø¿ª °³½Ã È¿À²À» °¨¼Ò½ÃŰ´Â ºÎºÐÀûÀ¸·Î °áÇÔÀ» °¡Áø IRES¸¦ Æ÷ÇÔÇÑ´Ù. À̰ÍÀº ¸ñÀû ´Ü¹éÁúÀ» °í¹ßÇöÇÏ´Â ¼¼Æ÷¸¦ ¿ì¼±ÀûÀ¸·Î ¼±º°ÇÒ ¼ö ÀÖ°Ô ÇÑ´Ù (3).
Living Colors pIRES2 Vectors¿Í ¾î¿ï¸®´Â À¯ÀüÀÚ ¹ßÇö
Living Colors pIRES2 Vector´Â ¿ÏÀüÈ÷ Ȱ¼ºÈ­µÈ IRES ¼­¿­À» Æ÷ÇÔÇϰí ÀÖ¾î Çü±¤´Ü¹éÁúÀ» µ¿µîÇÏ°Ô °í¹ßÇö½Ãų ¼ö ÀÖ´Ù. ¸¶Ä¿´Â À¶ÇմܹéÁúÀ̱⠺¸´Ù´Â °³º°ÀûÀÎ ´Ü¹éÁúÀ̱⠶§¹®¿¡ ¸ñÀû ´Ü¹éÁúÀÇ »ý¹°ÇÐÀû Ȱ¼º¿¡ ´ëÇÑ À§ÇèÀÌ °ÅÀÇ ¾ø´Ù. pIRES2 vector´Â ÀϽÃÀûÀ¸·Î transfectionµÈ ¼¼Æ÷ÀÇ ³óÃàÀ̳ª Çü±¤ °­µµ¸¦ ÅëÇØ ¸ñÀû À¯ÀüÀÚÀÇ ¹ßÇö ¼öÁØÀ» °üÂû Çϴµ¥ À¯¿ëÇÑ´Ù.
pEXP-Lib vector´Â Æ÷À¯·ù ¼¼Æ÷¿¡¼­ cDNA library¸¦ ¹ßÇö½Ã۴µ¥ »ç¿ëÇÒ ¼ö ÀÖ´Ù. 2 Á¾ÀÇ retrovirus¿ë IRES vector´Â transfectionÇϱâ Èûµç ¼¼Æ÷¿¡ ¸ñÀûÀ¯ÀüÀÚ¿Í Çü±¤´Ü¹éÁúÀ» µµÀÔÇϴµ¥ À¯¿ëÇÏ´Ù.


Figure 1. Map of the pIRES Selection Vectors.


Figure 2. Map of the Fluorescent pIRES2 Bicistronic Expression Vectors.


Figure 3. Schematic diagram of bicistronic mRNA translation. The internal ribosome entry site (IRES) permits a protein of interest and either an antibiotic resistance marker or fluorescent protein to be translated from the same mRNA. IVS = synthetic intron.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
References
1. Jackson, R. J. et al. (1990) Trends Biochem. Sci. 15:477-483.
2. Jang, S. K. et al. (1988) J. Virol. 62:2636-2643.
3. Rees, S. et al. (1996) Biotechniques 20:102-110.

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