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Home > ÀüÁ¦Ç°º¸±â > Real Time PCR > Intercalator TB Green °ËÃâ > External Standard Kit (¥ëpolyA) for qPCR
Real Time PCR¿ë ¿ÜºÎ Ç¥ÁØRNA£¦ÇÁ¶óÀ̸Ó

External Standard Kit (¥ëpolyA) for qPCR

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Takara
3789
External Standard Kit (¥ëpoly A) for qPCR
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 Set
270,400¿ø 
338,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
3789_e.v1903Da.pdf

Á¦Ç° ¼³¸í
ÀϹÝÀûÀ¸·Î Real Time PCR·Î À¯ÀüÀÚÀÇ ¹ßÇöÀ» È®ÀÎÇÏ´Â °æ¿ì reference geneÀ» »ç¿ëÇØ ¸ñÀû À¯ÀüÀÚÀÇ ¾çÀ» »ó´ëÀûÀ¸·Î Á¤·®ÇÑ´Ù. ÇÏÁö¸¸, ½ÇÇè¿¡ µû¶ó ÀÌ·¯ÇÑ house keeping geneÀ» ¼±Á¤Çϱ⠾î·Á¿î °æ¿ì Â÷¼±Ã¥À¸·Î External Standard Kit (λ poly A) for qPCR°ú °°Àº external standard¸¦ ÷°¡ÇØ reference·Î »ç¿ëÇÏ´Â °ÍÀÌ °¡´ÉÇÏ´Ù. ÀÌ Á¦Ç°Àº external standard·Î »ç¿ëÇϱâ À§ÇÑ RNA¿Í »ùÇà Èñ¼®¿ë¾× EASY Dilution (for Real Time PCR) (Code 9160) ¹× °ËÃâ¿ë primer ¼¼Æ®·Î ±¸¼ºµÇ¾î ÀÖ´Ù.
ÀÌ RNA´Â λ DNA¸¦ ÁÖÇüÀ¸·Î in vitro transcriptionÀ¸·Î ÇÕ¼ºÇÑ PolyA ¼­¿­À» °¡Áø ¾à 1.0kbÀÇ RNAÀ̸ç ÁøÇÙ»ý¹°ÀÇ »ùÇÿ¡¼­ external standard·Î »ç¿ëÇÒ ¼ö ÀÖ´Ù. 3’ ¸»´Ü¿¡ PolyA ¼­¿­À» Æ÷ÇÔÇÏ°í ÀÖ¾î oligo dT primer¸¦ ÅëÇÑ ¿ªÀü»ç ¹ÝÀÀ ¿ª½Ã °¡´ÉÇÏ´Ù.
Çü»ó

Real Time Primer for λ poly A*1

10 μM each

200 §¡

EASY Dilution (for Real Time PCR)*2

 

1 §¢

λ poly A£« RNA-A

10 ng/§¡

15 §¡

*1 ÁõÆø »ê¹°ÀÇ Å©±â´Â 88 bp ÀÌ´Ù.
*2 EASY Dilution (for Real Time PCR) (Code 9160) ´ÜÇ° ±¸ÀÔ °¡´É
º¸Á¸

Real Time Primer for λ poly A

-20¡É

EASY Dilution (for Real Time PCR)

-20¡É

λ poly A£« RNA-A

-80¡É

°ü·Ã Á¦Ç°
- 15ºÐ ¿Ï·á Real Time PCR¿ë cDNA ÇÕ¼º 5x premix, PrimeScript™ RT Master Mix (Perfect Real Time) (Code RR036A)
- Tli RNaseH°¡ Æ÷ÇԵǾî Á¤È®ÇÑ qPCR, TB Green® Premix Ex Taq™ II (Tli RNaseH Plus) (Codre RR820A)
- RTºÎÅÍ qPCR ±îÁö 1step, One Step TB Green® PrimeScript™ PLUS RT-PCR Kit (Perfect Real Time) (Code RR096A)
- gDNA Á¦°Å columnÀÌ Æ÷ÇÔµÈ RNA ÃßÃâ Kit, TaKaRa MiniBEST Universal RNA Extraction Kit (Code 9767A)

Keyword :

Intercalator TB Green °ËÃâ
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