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Home > ÀüÁ¦Ç°º¸±â > Real Time PCR > Dual-Labeled Probe °ËÃâ > gDNA Á¦°Å& RT ½Ã¾à (qPCR¿ë)
Genomic DNA Á¦°Å°úÁ¤ÀÌ Ãß°¡µÈ qPCR¿ë cDNA ÇÕ¼º kit

gDNA Á¦°Å& RT ½Ã¾à (qPCR¿ë)

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Takara
RR047A
PrimeScript¢â RT reagent Kit with gDNA Eraser (Perfect Real Time)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º  »ùÇýÅû 
100 ȸ
(20 §¡ ¹ÝÀÀ°è)
369,000¿ø  RR047A_e.v2202Da.pdf
Takara
RR047B
PrimeScript¢â RT reagent Kit with gDNA Eraser (Perfect Real Time)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
400 ȸ (RR047A x 4)
1,328,000¿ø 
Takara
9160
EASY Dilution (for Real Time PCR)
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 ml ¡¿ 8
229,000¿ø  9160_DS.v1902Da-a_116885.pdf



  • gDNA Eraser¿¡ ÀÇÇØ genomic DNA¸¦ 2ºÐ¸¸¿¡ Á¦°ÅÇÑ ÈÄ, 15ºÐ°£ ¿ªÀü»ç¹ÝÀÀÀ¸·Î qPCR¿ë cDNA¸¦ ÇÕ¼º°¡´É
  • 2Á¾·ùÀÇ ¿ªÀü»ç¿ë primer·Î Real Time PCR¿¡ ÃÖÀûÀÎ ÁÖÇü cDNA¸¦ ÇÕ¼º
  • TB Green¢â¿ë°ú Dual-Labeled probe assay¿ë ½ÇÇè¿¡ ¸ðµÎ Àû¿ë °¡´É
Á¦Ç°¼³¸í
º» Á¦Ç°Àº genomic DNA Á¦°Å°úÁ¤ÀÌ Ãß°¡µÈ Real Time RT-PCR¿ë ¿ªÀü»ç ½Ã¾àÀÌ´Ù.
°­·ÂÇÑ DNA ºÐÇØ È°¼ºÀ» °¡Áø gDNA Eraser¿¡ ÀÇÇØ genomic DNA¸¸À» 42¡É, 2ºÐÀÇ Ã³¸®·Î Á¦°ÅÇÑ ÈÄ, DNA ºÐÇØ È°¼ºÀ» ¿ÏÀüÇÏ°Ô ¾ïÁ¦ÇÏ´Â ¼ººÐÀ» Æ÷ÇÔÇÑ ¿ªÀü»ç¿ë ¹ÝÀÀ ½Ã¾àÀ»
÷°¡ÇÏ¿© 15ºÐ°£ÀÇ ¿ªÀü»ç ¹ÝÀÀÀ» ½Ç½ÃÇÑ´Ù. 20ºÐ ¹Ì¸¸ÀÇ ½Å¼ÓÇÑ ¹ÝÀÀÀ¸·Î »ùÇÃÀÇ ¼Õ½Ç¾øÀÌ RNA·ÎºÎÅÍ qPCR¿ë cDNA¸¦ ÇÕ¼ºÇÒ ¼ö ÀÖ´Ù.
º» Á¦Ç°¿¡ ÀÇÇØ ÇÕ¼ºµÈ cDNA´Â TB Green¢â assay, Dual-Laveled Probe assay¿¡ ¸ðµÎ »ç¿ëÇÒ ¼ö ÀÖÀ¸¸ç, ¸ñÀû¿¡ µû¶ó TB Green¢â Premix Ex Taq¢â II (Tli RNaseH Plus) (Code RR820A), Probe qPCR Mix (Code RR391A) µî Real Time PCR ½Ã¾à°ú ÇÔ²² »ç¿ëÇÑ´Ù.
³»¿ë (100 ȸ, 20 §¡ ¹ÝÀÀ½Ã)

1.

gDNA Eraser

100 §¡

2.

5¡¿gDNA Eraser Buffer *1

200 §¡

3.

PrimeScript RT Enzyme Mix I *2

100 §¡

4.

5¡¿PrimeScript Buffer 2 (for Real Time) *3

400 §¡

5.

RT Primer Mix *4

400 §¡

6.

RNase Free dH2O

1 §¢ x 2

7.

EASY Dilution (for Real Time PCR) *5

1 §¢


*1 5¡¿gDNA Eraser Buffer ¼ººÐÀÌ ±× ÈÄÀÇ ¿ªÀü»ç ¹ÝÀÀ¿¡ ÇÊ¿äÇϱ⠶§¹®¿¡ genomic DNA Á¦°Å ¹ÝÀÀÀº ¹Ýµå½Ã ÁøÇàÇÑ´Ù.
*2 RNase inhibitor¸¦ Æ÷ÇÔÇÑ´Ù.
*3 dNTP mixture¸¦ Æ÷ÇÔÇÑ´Ù.
*4 Oligo dT Primer ¿Í Random 6 mers¸¦ Æ÷ÇÔÇÑ´Ù.
*5 total RNA³ª cDNA¸¦ ´Ü°è Èñ¼®ÇÒ ¶§ Èñ¼®¿ë¾×À¸·Î »ç¿ëÇÑ´Ù. H2O³ª TE buffer·Î Èñ¼®Çϸé Á¤È®ÇÑ Èñ¼®À» ÇÒ ¼ö ¾ø´Â °æ¿ì°¡ ÀÖÁö¸¸, EASY DilutionÀ» ÀÌ¿ëÇϸé Àú³óµµ±îÁö Á¤È®È÷ Èñ¼®À» ÇÒ ¼ö ÀÖ´Ù. º» buffer´Â ¿ªÀü»ç ¹ÝÀÀÀ̳ª PCRÀÇ ¹ÝÀÀ¼º¿¡ ¿µÇâÀ» ¹ÌÄ¡Áö ¾ÊÀ¸¹Ç·Î Èñ¼®ÇÑ ÁÖÇü ¿ë¾×À» ±×´ë·Î ¿ªÀü»ç ¹ÝÀÀÀ̳ª PCR ¹ÝÀÀÀÇ ÁÖÇüÀ¸·Î¼­ »ç¿ëÇÒ ¼ö ÀÖ´Ù.
EASY Dilution (for Real Time PCR) (Code 9160)´Â º°µµ ±¸¸Å °¡´É.

¡¼ÁÖÀÇ¡½EASY DilutionÀº TakaraÀÇ Real Time PCR ½Ã¾à°ú ÇÔ²² »ç¿ëÇϱ⸦ ±ÇÀåÇϸç, Ÿ»ç Á¦Ç°°úÀÇ È£È¯¼ºÀº È®ÀεÇÁö ¾Ê¾Ò½À´Ï´Ù.
º¸Á¸ -20¡É
ÇÔ²² »ç¿ëÇÏ´Â Á¦Ç°
<cDNA ÇÕ¼ººÎÅÍ TB Green¢â premix±îÁö ÇÔ²² »ç¿ëÇϸé Perfect Real Time PCR>

<Reference paper list>
³í¹®Á¦¸ñ

³í¹®¸í

»ç¿ëÁ¦Ç°

Identification and Comparative Analysis of microRNA in Wheat (Triticum aestivum L.) Callus Derived from Mature and Immature Embryos during In vitro Culture

Front Plant Sci. 2016; 7: 1302.

RR047,
RR820

Identification of Putative ORF5 Protein of Porcine Circovirus Type 2 and Functional Analysis of GFP-Fused ORF5 Protein

PLoS One. 2015; 10(6): e0127859.

RR047,
RR820

Glucagon-like peptide-1 protects cardiomyocytes from advanced oxidation protein product-induced apoptosis via the PI3K/Akt/Bad signaling pathway

Mol Med Rep. 2016 Feb; 13(2): 1593?1601.

RR047,
RR820

Genome-wide identification of vegetative phase transition-associated microRNAs and target predictions using degradome sequencing in Malus hupehensis

BMC Genomics. 2014; 15(1): 1125.

RR047,
RR820

Antennal transcriptome analysis of the Asian longhorned beetle Anoplophora glabripennis

Sci Rep. 2016; 6: 26652.

RR047,
RR820

Identification of heat-responsive genes in carnation (Dianthus caryophyllus L.) by RNA-seq

Front Plant Sci. 2015; 6: 519.

RR047,
RR820

Heterologous Reconstitution of Omega-3 Polyunsaturated Fatty Acids in Arabidopsis

Biomed Res Int. 2015; 2015: 768478.

RR047,
RR820

ÁÖÀÇ
º» Á¦Ç°À» ÀÌ¿ëÇÏ¿© ÇÕ¼ºµÈ cDNA·Î TB Green¢â °ËÃâ¿ë Real Time RT-PCR ºÐ¼®À» ½Ç½ÃÇÏ´Â °æ¿ì¿¡´Â ¾Æ·¡ µÎ Á¦Ç°ÀÇ »ç¿ëÀ» ±ÇÀåÇÕ´Ï´Ù.
º» Á¦Ç°°ú Á¶ÇÕÇؼ­ »ç¿ëÇÔÀ¸·Î½á ½Å·Ú¼º ³ôÀº °á°ú¸¦ ¾òÀ» ¼ö ÀÖ½À´Ï´Ù.

´Ü, TB Green¢â Fast qPCR Mix (Code RR430A/B)¿ÍÀÇ Á¶ÇÕÀº ÃßõÇÏÁö ¾Ê½À´Ï´Ù.

Keyword : Dnase I,gDNAÁ¦°Å,Real time pcr,qpcr,sybr, sybr green,cDNAÇÕ¼º,rt kit

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