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Home > 전제품보기 > Real Time PCR > TB Green 검출(Clontech) > TB Green® Advantage qPCR Premix

TB Green® Advantage qPCR Premix

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제조사 제품코드 제품명 용량 가격
(부가세별도)		 비고 사용자매뉴얼
Clontech
 639676
 TB Green® Advantage qPCR Premix
관련학술 구매하기 		200 rxns
 311,000원  제조사 페이지로 바로가기 x100175, x100174


TB Green Advantage qPCR Premix-Taq Master Mix with Hot Start & TB Green

 TB Green Advantage qPCR Premix is a convenient, ready-to-use 2X-concentrated master mix that contains full-length Taq polymerase with hot start antibody and TB Green I, and is specially designed for real-time PCR employing the dye intercalator method. This product combines the high performance of our enzyme for hot start PCR utilizing Taq antibody and a newly developed buffer, to provide superior specificity, increased amplification efficiency, and excellent performance in high-speed, real-time PCR. Use of the TB Green Advantage qPCR Premix enables you to carry out successful real-time PCR with high sensitivity, broad dynamic range, and accurate quantification. The reagent is supplied separately with ROX Reference Dye LSR and ROX Reference Dye LMP, which allow you to normalize the fluorescent signal between reactions on instruments that are equipped with this option. Recent studies have shown that the Clontech TB Green Advantage qPCR Premix is superior to corresponding reagents offered by three leading competitors in the qPCR arena, in terms of reaction specificity, amplification efficiency, and sensitivity.

 More Specific than Competitor R
Competitor R’s real-time enzyme showed poor reaction specificity when compared to the Clontech TB Green Advantage qPCR Premix. This was evidenced by multiple peaks in the melting curve for Competitor R's enzyme, particularly when low-copy-number templates were amplified (Figure 1).

More Efficient than Competitor A
Competitor A's TB Green showed a lower amplification efficiency than that of the Clontech TB Green Advantage qPCR Premix. This was indicated by Ct values that were shifted to the right (Figure 2).

More Specific than Competitor I
The Clontech TB Green Advantage qPCR Premix demonstrated superior reaction specificity when compared to Competitor I's mix. This was indicated by tighter peaks in the melting curve for the Clontech enzyme (Figure 3).

Twice as Fast as Competitor A
Excellent results were obtained in about half the time (50 min versus 90 min) when using the Clontech TB Green Advantage qPCR Premix as opposed to Competitor A's TaqMan mix (data not shown). These experiments were carried out with the Applied Biosystems 7500 Real-Time PCR System.

Clearly, the Clontech TB Green Advantage qPCR Premix outperforms much of the competition in terms of specificity (melting curves indicate the presence of a single product), efficiency, and sensitivity (lower Ct values in the amplification plots). Moreover, when using the Clontech TB Green Advantage qPCR Premix, qPCR analysis can be accomplished in nearly half of the time required for other qPCR chemistries.

For GC-Rich Targets
Use TB Green Advantage GC qPCR Premix to amplify difficult, GC-rich targets. This 2X master mix offers the same advantages and benefits as the TB Green Advantage qPCR Premix, but it’s optimized for targets with a GC content between 60-70%.

Compatible cyclers:
SmartCycler, LightCycler, ABI PRISM 7000/7700/7900 HT, Applied Biosystems 7300/7500, iCycler, Opticon, Stratagene MX 3000P, and Rotor-Gene.

Components
TB Green Advantage qPCR Premix
2X TB Green Advantage qPCR Premix
50X ROX Reference Dye LSR
50X ROX Reference Dye LMP

TB Green Advantage GC qPCR Premix
2X TB Green Advantage GC qPCR Premix
50X ROX Reference Dye LSR
50X ROX Reference Dye LMP
Storage Conditions
  • Store at -70℃ in a dark container
  • After thawing, store at 4℃ in a dark container - Do not refreeze!

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