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Home > ÀüÁ¦Ç°º¸±â > Real Time PCR > Dual-Labeled Probe °ËÃâ > Probe qPCR Mix (¶Ç´Â UNG Æ÷ÇÔ)
´Ù¾çÇÑ »ùÇÿ¡ Àû¿ë¼ºÀÌ ³ôÀº Dual-Labeled Probe °ËÃâ Àü¿ë½Ã¾à

Probe qPCR Mix (¶Ç´Â UNG Æ÷ÇÔ)

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
RR391A
Probe qPCR Mix
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
200 ȸ
(50 §¡ ¹ÝÀÀ½Ã)
254,400¿ø 
318,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR391A_e.v2201Da.pdf
Takara
RR391B
Probe qPCR Mix
°ü·ÃÇмú ±¸¸ÅÇϱâ
400 ȸ (RR391A x 2)
572,000¿ø 
Takara
RR392A
Probe qPCR Mix, with UNG
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
200 ȸ
(50 §¡ ¹ÝÀÀ°è)
266,400¿ø 
333,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
RR392A_RR392S_e.v2207Da.pdf
Takara
RR392B
Probe qPCR Mix, with UNG
°ü·ÃÇмú ±¸¸ÅÇϱâ
400 ȸ (RR392A x 2)
600,000¿ø 



  • PCR ¾ïÁ¦¹°Áú¿¡ ´ëÇÑ ÀúÇ×¼ºÀ» °­È­
  • ±âº» ÇÁ·ÎÅäÄÝ°ú °í¼Ó ÇÁ·ÎÅäÄÝ¿¡ ¸ðµÎ »ç¿ë°¡´É
  • 2¡¿ premix¿¡ ³»¿­¼º RNaseH ÷°¡ - ÀÜÁ¸ mRNA¿¡ ÀÇÇÑ PCR ÀúÇعÝÀÀ ¾ïÁ¦
  • Carryover contamination ¹æÁö¸¦ À§ÇÑ UNG plus ¹öÀü ¼±Åà °¡´É (RR392A/B)
Á¦Ç°¼³¸í
Probe qPCR Mix´Â Dual-Labeled Probe¹ýÀ» ÀÌ¿ëÇÑ Real Time PCR Àü¿ë ½Ã¾àÀÌ´Ù. Anti-Taq Ç×ü¸¦ ÀÌ¿ëÇÑ hot start PCR È¿¼Ò¿Í Real Time PCR¿ë buffer¸¦ °³·®ÇÏ¿© ÀϹÝÀûÀÎ »ùÇÃÀÇ ÁõÆø»Ó¸¸ ¾Æ´Ï¶ó, PCR ¾ïÁ¦¹°ÁúÀÌ Æ÷ÇÔµÈ »ùÇÿ¡¼­µµ ³ôÀº ƯÀ̼º°ú ÁõÆø È¿À², ¶Ù¾î³­ °ËÃâ °¨µµ·Î ÁõÆøÇÒ ¼ö ÀÖ´Ù.
2¡¿ ³óµµÀÇ premix type½Ã¾àÀ¸·Î ¹ÝÀÀ¾× Á¶Á¦°¡ °£´ÜÇÏ°í, ³»¿­¼º RNaseHÀÎ Tli RNaseH °¡ 2¡¿ Premix ½Ã¾à Áß¿¡ ÷°¡µÇ¾î ÀÖ¾î cDNA¸¦ ÁÖÇüÀ¸·Î ÇÑ °æ¿ì ÀÜÁ¸ mRNA¿¡ ÀÇÇÑ PCR ¹ÝÀÀ ÀúÇظ¦ ±ØÇÑÀ¸·Î ¾ïÁ¦ÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ °í¼Ó ÇÁ·ÎÅäÄݵµ »ç¿ëÇÒ ¼ö ÀÖÀ¸¸ç, ÀçÇö¼º ÀÖ°Ô Æø³ÐÀº ¹üÀ§¿¡¼­ º¸´Ù Á¤È®ÇÑ Å¸°ÙÀÇ Á¤·® ¹× °ËÃâÀ» ÁøÇàÇÒ ¼ö ÀÖ´Ù.
Probe qPCR Mix, with UNG´Â ¹Ýº¹ÀûÀÎ PCR ¹ÝÀÀ¿¡¼­ ¹ß»ý À§ÇèÀÌ ³ôÀº carryover contaminationÀ» ¹æÁöÇϱâ À§ÇØ Uracil-N-Glycosylase (UNG)¸¦ Æ÷ÇÔÇÏ°í ÀÖ´Ù.
³»¿ë
Probe qPCR Mix (Code RR391A, 50 §¡ ¹ÝÀÀ½Ã 200ȸ ºÐ·®)
1.

Probe qPCR Mix (2¡¿conc.)*1

1 §¢¡¿ 5°³

2.

ROX Reference Dye (50¡¿conc.)*2

200 §¡

3.

ROX Reference Dye II (50¡¿conc.)*2

200 §¡


Probe qPCR Mix, with UNG (Code RR392A, 50 §¡ ¹ÝÀÀ½Ã 200ȸ ºÐ·®)
1.

Probe qPCR Mix, with UNG (2¡¿conc.)*3

1 §¢¡¿ 5°³

2.

ROX Reference Dye (50¡¿conc.)*2

200 §¡

3.

ROX Reference Dye II (50¡¿conc.)*2

200 §¡


*1 PCR È¿¼Ò, dNTP Mixture, Mg2£«, Tli RNase H¸¦ Æ÷ÇÔÇÑ´Ù.
*2 Life TechnologiesÀÇ Real Time PCR ÀåÄ¡ µî well °£ Çü±¤ ½ÅÈ£¸¦ º¸Á¤ÇÏ´Â ÀåÄ¡·Î Çؼ®ÇÏ´Â °æ¿ì¿¡ »ç¿ëÇÑ´Ù.
  • ROX Reference Dye(50¡¿)À» ÷°¡ÇÏ´Â ±âÁ¾
    Applied Biosystems 7300 Real-Time PCR System (Life Technologies)
    StepOnePlus Real-Time PCR System (Life Technologies)

  • ROX Reference Dye II(50¡¿)À» ÷°¡ÇÏ´Â ±âÁ¾
    Applied Biosystems 7500/7500 Fast Real-Time PCR System (Life Technologies)

  • ÷°¡°¡ ÇÊ¿ä ¾ø´Â ±âÁ¾
    CronoSTAR¢â 96 Real-Time PCR System (4ch) (Code 640231)
    Thermal Cycler Dice Real Time System III (Code TP950/TP970/TP980)
    Thermal Cycler Dice Real Time System II (Code TP900/TP960)
    Thermal Cycler Dice Real Time System Single (Code TP850/TP870)
    Thermal Cycler Dice Real Time System Lite (Code TP700/TP760)
    Smart Cycler System/Smart Cycler II System (Cepheidȍ)
    LightCycler 96/LightCycler 480 System (Roche Diagnosticsȍ)
    CFX96 Real-Time PCR Detection System (Bio-Radȍ)
*3 PCR È¿¼Ò, dNTP Mixture, Mg2£«, Tli RNase H, UNG ¸¦ Æ÷ÇÔÇÑ´Ù.
º¸Á¸
4¡É¿¡ º¸°üÇÏ´Â °æ¿ì 6°³¿ù ¾ÈÁ¤
¡Ø ¿À¿°¿¡ ÁÖÀÇÇϽʽÿÀ.

- Àå±â º¸Á¸ÀÇ °æ¿ì´Â -20 ¡É¿¡¼­ º¸°üÇϽʽÿÀ.
- ÀÏ´Ü ¿ëÇØ ÈÄ¿¡´Â 4¡É º¸°üÇÏ°í, 6°³¿ù¾È¿¡ »ç¿ëÇÒ °ÍÀ» ±ÇÀåÇÕ´Ï´Ù.

Keyword : Taqman,probe,dual labeled,taqman probe,qPCR,Real time pcr

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