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Home > ÀüÁ¦Ç°º¸±â > Real Time PCR > CycleavePCR °ËÃâ > CycleavePCR¢â Reaction Mix
Cycling probe Àü¿ë qPCR premix ½Ã¾à

CycleavePCR¢â Reaction Mix

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Takara
CY505S
CycleavePCR Starter Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
80 ȸ
106,400¿ø 
133,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
Takara
CY505A
CycleavePCR Reaction Mix
°ü·ÃÇмú ±¸¸ÅÇϱâ
400ȸ
458,400¿ø 
573,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
Takara
CY505B
CycleavePCR Reaction Mix
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1,200ȸ (CY505A x 3)
1,632,000¿ø 

  • 2X premix·Î Æí¸®ÇÑ »ç¿ë
  • SNP genotyping¿¡ ÃÖÀû
Á¦Ç°¼³¸í
CycleavePCR™ Reaction Mix´Â Cycling probe¸¦ ÀÌ¿ëÇÑ °ËÃâ½Ã »ç¿ëÇÏ´Â Real Time PCR ½Ã¾àÀ¸·Î, ÃÖÀûÈ­µÈ buffer¿Í TaKaRa Ex Taq HS PCRÈ¿¼Ò°¡ 2X premix·Î Á¦À۵Ǿî Æí¸®ÇÏ°Ô »ç¿ëÇÒ ¼ö ÀÖ´Ù. ÀÌ·¯ÇÑ Cycling probe¹ýÀ» »ç¿ëÇϸé RT-qPCR·Î Ÿ°ÙÀÇ °ËÃâ ¹× Á¤·® »Ó ¾Æ´Ï¶ó ´ÜÀÏ¿°±â´ÙÇü¼º (SNP) °ËÃâ±îÁö Á¤È®ÇÏ°í °£ÆíÇÏ°Ô ÁøÇàÇÒ ¼ö ÀÖ´Ù.
CycleavePCR™ Starter Kit´Â CycleavePCR Reaction Mix¿¡ positive control ½ÇÇèÀ» À§ÇÑ ½Ã¾àÀÌ Ãß°¡µÇ¾î ÀÖ´Ù. ÀÌ¹Ì ½Ã½ºÅÛÀ» ±¸ÃàÇß´Ù¸é CycleavePCR™ Reaction Mix (Code CY505A)¸¦, óÀ½ ½ÇÇèÀ» ½ÃÀÛÇÑ´Ù¸é CycleavePCR Starter Kit (Code CY505S)¸¦ ÃßõÇÑ´Ù.
¡Ø ÁÖÀÇ: dual-labeled probe¸¦ »ç¿ëÇÑ RT-PCR¿¡´Â Premix Ex Taq™ (Probe qPCR) (Code RR390A ¿Ü)¸¦, intercalator¸¦ »ç¿ëÇÑ RT-PCR¿¡´Â TB Green® Premix Ex Taq™ II (Tli RNaseH Plus) (Code RR820A ¿Ü) ¶Ç´Â TB Green® Premix Ex Taq™ (Tli RNaseH Plus) (Code RR420A ¿Ü)¸¦ ÃßõÇÑ´Ù.
Àû¿ë °¡´ÉÇÑ PCR ±â±â
ÀÌ Á¦Ç°Àº ¾Æ·¡ÀÇ °¢Á¾ PCR±â±â¿ÍÀÇ È£È¯¼ºÀÌ È®ÀεǾú´Ù.
  - CronoSTAR 96 Real-Time PCR System (Code 640231 ¿Ü)
  - Thermal Cycler Dice Real Time System III (Code TP950 ¿Ü)
  - Appied Biosystems 7300/7500/7500 Fast Real-Time PCR System
  - StepOnePlus Real-Time PCR System (Thermo Fisher Scientific)
  - LightCycler (Roche Diagnostics»ç)
ÀÌ¿Ü¿¡µµ ÀϹÝÀûÀÎ qPCR ±â±â¿Í ÇÔ²² »ç¿ëÇÒ ¼ö ÀÖ´Ù.
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CycleavePCR Starter Kit (Code CY505S) (25 §¡ ¹ÝÀÀ¾×, 80ȸºÐ)

CycleavePCR Reaction Mix (2×conc.)*1

1 §¢

ROX Reference Dye (50×conc.)*2

200 §¡

ROX Reference Dye II (50×conc.)*2

200 §¡

Positive Control*3

10 §¡

Positive Control Primer Mix (10 μM each)*3

10 §¡

Positive Control Probe (FAM/TAMRA) (25×conc.)*3 *4

10 §¡

dH2O

1 §¢


CycleavePCR Reaction Mix (Code CY505A) (25 §¡ ¹ÝÀÀ¾×, 400ȸºÐ)

CycleavePCR Reaction Mix (2×conc.)*1

5x 1 §¢

ROX Reference Dye (50×conc.)*2

200 §¡

ROX Reference Dye II (50×conc.)*2

200 §¡


*1 TaKaRa Ex Taq HS, dNTP Mixture ¹× Mg2+ Tli RNaseH II°¡ Æ÷ÇԵǾî ÀÖ´Ù.
*2 Applied BiosystemµîÀÇ ÀϺΠqPCR±â±â¿¡¼­ well °£ÀÇ ½ÅÈ£¸¦ º¸Á¤Çϴµ¥ »ç¿ëÇÑ´Ù.

ROXÇ¥½Ä probe¸¦ »ç¿ëÇÏ´Â °æ¿ì¿¡´Â »ç¿ëÀÌ ºÒ°¡´ÉÇÏ´Ù.
  • ROX reference dye¸¦ »ç¿ëÇÏ´Â qPCR±â±â
    - Applied Biosystems 7300 Real-Time PCR System(Thermo Fisher Scientific)
    - StepOnePlus Real-Time PCR System(Thermo Fisher Scientific)
  • ROX reference dye II¸¦ »ç¿ëÇÏ´Â qPCR ±â±â
    - Applied Biosystems 7500/7500 Fast Real-Time PCR System(Thermo Fisher Scientific)
  • Rox reference dyeÀÇ Ã·°¡°¡ ÇÊ¿ä ¾ø´Â ±â±â
    - CronoSTAR 96 Real-Time PCR System (Code 640231 ¿Ü)
    - Thermal Cycler Dice Real Time System½Ã¸®Áî (Code TP950 ¿Ü)
    - LightCycler (Roche Diagnostics)
    ÀÚ¼¼ÇÑ ROX reference dye »ç¿ë¿¡ ´ëÇÑ ³»¿ëÀº °¢ ±â±âÀÇ Á¦Á¶»ç¿¡ ¹®ÀÇ ¹Ù¶ø´Ï´Ù.

*3 ½Ã¾àÀÇ ¹ÝÀÀ¼ºÀ» È®ÀÎÇϱâ À§ÇÑ positive control ½ÇÇè¿ëÀÌ´Ù.
*4 Çü±¤Ç¥Áö probe (FAM/TAMRA)¸¦ Æ÷ÇÔÇÏ°í ÀÖÀ¸¹Ç·Î, Â÷±¤ÇÏ¿© º¸°üÇÑ´Ù.
º¸Á¸ -20¡É
°ü·ÃÁ¦Ç°
- spin columnÀ» »ç¿ëÇÑ gDNA ÃßÃâ Kit, TaKaRa MiniBEST Universal Genomic DNA Extraction Kit (Code 9765A)
- gDNA Á¦°Å columnÀÌ Æ÷ÇÔµÈ RNA ÃßÃâ Kit, TaKaRa MiniBEST Universal RNA Extraction Kit (Code 9767A)
- AGPC¹ýÀ» È°¿ëÇÑ RNA Á¤Á¦ Kit, RNAiso Plus (Code 9109 ¿Ü)
- qPCR¿ë cDNA 15ºÐ ÇÕ¼º 5X premix, PrimeScript™ RT Master Mix (Perfect Real Time) (Code RR036A)

Keyword :

CycleavePCR °ËÃâ
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Cycling Probe ¹ýÀ» ÀÌ¿ëÇÑ Real Time PCR ¿ë ..
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