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Inducible System ¡¤ Yeast hybrid
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Yeast One-Hybrid (Y1H)
> Yeast One-Hybrid (Y1H) System
Yeast One-Hybrid (Y1H) System
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Clontech
630491
Matchmaker Gold Yeast One-Hybrid Library Screening System
1 Set
(Package)
3,639,000¿ø
,
,
,
,
,
Clontech
630496
Matchmaker Insert Check PCR Mix 1
100 ȸ
352,000¿ø
Clontech
630497
Matchmaker Insert Check PCR Mix 2
100 ȸ
352,000¿ø
Product index
Tet À¯µµ ¹ßÇö ½Ã½ºÅÛ
[°³¿ä] Tet-on system
[¼±Åð¡À̵å] Tet Syste..
Tet-On 3G À¯µµ ¹ßÇö ½Ã..
Lenti-X Tet-On 3G Indu..
Tet-On 3G Cell Line
Tet Approved FBS
Tet °ü·Ã Ç×»ýÁ¦
Selection marker (Line..
Tet-One Inducible Expr..
TetR Ç×ü
[Legacy] Tetracycline ..
Tet-Off Cell Lines
pTRE-Tight Vectors
pTet-tTS Vectors
IRES Tet À¯µµ¹ßÇö ½Ã½º..
iDimerize Inducible System..
[°³¿ä] Inducible Prote..
iDimerize Antibodies
Inducible Expression S..
Heterodimer System
Homodimer System
Regulated Transcriptio..
Reverse Dimerization S..
´Ü¹éÁú ¹ßÇö·® Á¶Àý ½Ã½ºÅÛ ..
ProteoTuner System
Tet-ProteoTuner Vector..
[°³¿ä] Yeast Hybrid System
[FAQ] Matchmaker Gold ..
[°³¿ä] Matchmaker Gold..
Yeast Two Hybrid System (Y..
Y2H Library Constructi..
Yeast Culture media (T..
Yeast Two-Hybrid (Y2H)..
Y2H Library (Yeast ³»..
Normalized Y2H Library..
Yeast Two hybrid vecto..
pBridge Vector
Yeast One-Hybrid (Y1H)
Yeast Culture Media (O..
Yeast One-Hybrid (Y1H)..
Matchmaker °ü·ÃÁ¦Ç°
Yeast Culture Media
Yeast Culture Media (..
Aureobasidin A
Yeast Plasmid Isolati..
Matchmaker Ç×ü
Insert Check Kit (prey..
Yeast Transformation M..
X-¥á-Gal
Yeast Transformation S..
Mammalian Cell Assay K..
°¡Àå °íÈ¿À²ÀûÀÎ yeast one-hybrid ½Ã½ºÅÛ
»õ·Î¿î Protein-DNA °£ÀÇ »óÈ£ÀÛ¿ë È®ÀÎ
Aureobasidin A screening °úÁ¤ Ãß°¡·Î background °¨¼Ò
Yeast¿¡¼ Á÷Á¢ SMART cDNA library Á¦ÀÛ ¹× screening °¡´É
Aureobasidin A·Î ³·Àº background ½ÇÇö
Matchmaker Gold Yeast One-Hybrid Library Screening SystemÀº ¹ÌÁöÀÇ ´Ü¹éÁú°ú DNA °£ÀÇ »óÈ£ÀÛ¿ëÀ» È®ÀÎÇÏ°í ±× ±â´ÉÀ» ÆľÇÇÒ ¼ö ÀÖ´Â °£´ÜÇÏ°í È¿À²ÀûÀÎ ¹æ¹ýÀ» Á¦°øÇÑ´Ù (Figure 1). ¸ðµç Matchmaker Gold SystemÀº Á¤È®ÇÏ°í »ç¿ëÇϱâ Æí¸®ÇÑ reporter·Î Aureobasidin A ³»¼º (AbA
r
)À» »ç¿ëÇÑ´Ù. ÀÌ »õ·Î¿î reporter´Â Ç×»ýÁ¦ÀÎ Aureobasidin A (AbA)·Î ÀÎÇØ AbA ³»¼ºÀ¯ÀüÀÚ¸¦ ¹ßÇöÇÏÁö ¸øÇÏ´Â yeast¸¦ È¿°úÀûÀ¸·Î Á¦°ÅÇϱ⠶§¹®¿¡ ¸Å¿ì ³·Àº screening background¸¦ °¡´ÉÇÏ°Ô ÇÏ¿´´Ù.
Matchmaker Gold One-Hybrid System
Matchmaker Gold Yeast One-Hybrid system¿¡¼´Â ¸ñÀû DNA (bait) ¼¿À» ¸î ¹ø ¹Ýº¹ÇÏ¿© pAbAi reporter vector¿¡ cloning ÇÑ ÈÄ, bait-specific reporter¸¦ °¡Áø yeast strainÀ» ¸¸µé±â À§ÇÏ¿© pAbAi vector¸¦ homologous recombinationÀ¸·Î Y1HGold yeast genomeÀ¸·Î »ðÀÔ½ÃÄÑ library screeningÀ» À§ÇÑ ¼÷ÁÖ·Î »ç¿ëÇÑ´Ù.
Figure 1. Screening for protein-DNA interactions with the Matchmaker Gold One-Hybrid System.
One to three copies of the DNA target sequence are cloned into the pAbAi reporter vector, which is then integrated into the Y1HGold genome to create a bait-specific reporter strain. Activation of the AbA resistance gene (AbA
r
) occurs if a prey protein from the library binds to the bait sequence.
One-step Library Á¦ÀÛ°ú Screening
Yeast GAL4-transcription activation domain (GAL4 AD prey protein)°ú À¶ÇÕµÇ¾î ¹ßÇöµÇ´Â DNA-binding proteinÀ» Æ÷ÇÔÇÏ´Â cDNA library´Â SMART ±â¼ú°ú homologous recombinationÀ» ÀÌ¿ëÇÏ¿© Y1HGold [Bait-AbAi] reporter strain¿¡¼ Á÷Á¢ Á¦ÀÛÇÒ ¼ö ÀÖ´Ù. (Figure 2). Prey ´Ü¹éÁúÀÌ bait sequence¿Í °áÇÕÇϸé GAL4 AD´Â AbA ÀúÇ×¼º À¯ÀüÀÚÀÇ ¹ßÇöÀ» È°¼ºÈ½ÃÄÑ AbA¸¦ Æ÷ÇÔÇÏ´Â ¹èÁö¿¡¼ yeast°¡ »ýÁ¸ÇÏ°Ô µÈ´Ù. µû¶ó¼ library screening °úÁ¤¿¡¼ »ì¾Æ³²Àº yeast cloneÀ¸·ÎºÎÅÍ prey ´Ü¹éÁúÀ» ¹ßÇöÇÏ´Â plasmid¸¦ ½±°Ô ȹµæÇÒ ¼ö ÀÖ°í À̸¦ ÀÌ¿ëÇÏ¿© ´ÙÀ½ °úÁ¤ÀÇ ½ÇÇèÀ» ÁøÇàÇÒ ¼ö ÀÖ°Ô µÈ´Ù.
Colony PCR Kit & Media Set
Colony PCRÀº bait strainÀ» È®ÀÎÇÏ°í screeningÀ» ÅëÇØ È®ÀÎµÈ positive cloneÀ» ºÐ·ùÇϱâ À§ÇÑ °¡Àå ºü¸£°í Æí¸®ÇÑ ¹æ¹ýÀÌ´Ù. Matchmaker Insert Check PCR Mix 1Àº pBait-AbAi ÀçÁ¶ÇÕü°¡ yeast genome¿¡ »ðÀԵǾú´ÂÁö È®ÀÎÇϱâ À§ÇÏ¿© »ç¿ëÇϸç, Matchmaker Insert Check PCR Mix 2´Â onehybrid ¶Ç´Â two-hybrid screening °úÁ¤¿¡¼ ¾òÀº positive cloneÀ» ºü¸£°Ô ºÐ¼®Çϱâ À§ÇÏ¿© »ç¿ëÇÑ´Ù. Yeast Media Sets 1°ú 1 Plus´Â Matchmaker Gold One-Hybrid ½ÇÇè¿¡ ÇÊ¿äÇÑ ¹èÁö¸¦ pouch ÇüÅ·Π±¸¼ºÇÑ Á¦Ç°ÀÌ´Ù.
Figure 2. Use SMART technology and yeast biology to simultaneously construct and screen your library directly in yeast.
SMART technology creates a pool of cDNA harboring ends that are homologous to the linearized prey vector, pGADT7-Rec. The cDNA pool and pGADT7-Rec are cotransfomed into your Y1HGold-Bait reporter strain, and undergo homologous recombination within the yeast. Yeast cells are then plated on SD/-Leu/+AbA to select for colonies that express reporters as a result of positive Y1H interactions.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
Keyword :
Yeast One-Hybrid (Y1H)
Yeast Culture Media (One-Hybrid ¿ë)
Yeast One-Hybrid (Y1H) System