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Inducible System ¡¤ Yeast hybrid
>
Tet À¯µµ ¹ßÇö ½Ã½ºÅÛ
> Tet-One Inducible Expression (One vector System)
All-in-one vector ÇüÅÂÀÇ Tet-inducible system
Tet-One Inducible Expression (One vector System)
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Clontech
634301
Tet-One Inducible Expression System
Each
2,322,000¿ø
,
Clontech
631844
Lenti-X¢â Tet-One¢â Inducible Expression System
Each
(Package)
3,551,000¿ø
Clontech
631847
Lenti-X¢â Tet-One¢â Inducible Expression System (Puro)
Each
(Package)
3,551,000¿ø
Clontech
634304
Retro-X Tet-One Inducible Expression System
Each
(Package)
3,181,000¿ø
,
Clontech
634307
Retro-X Tet-One Inducible Expression System (puro)
Each
(Package)
3,181,000¿ø
,
Clontech
634310
AAVpro¢ç Tet-One Inducible Expression System (AAV2)
Each
1,827,000¿ø
,
Product index
Tet À¯µµ ¹ßÇö ½Ã½ºÅÛ
[°³¿ä] Tet-on system
[¼±Åð¡À̵å] Tet Syste..
Tet-On 3G À¯µµ ¹ßÇö ½Ã..
Lenti-X Tet-On 3G Indu..
Tet-On 3G Cell Line
Tet Approved FBS
Tet °ü·Ã Ç×»ýÁ¦
Selection marker (Line..
Tet-One Inducible Expr..
TetR Ç×ü
[Legacy] Tetracycline ..
Tet-Off Cell Lines
pTRE-Tight Vectors
pTet-tTS Vectors
IRES Tet À¯µµ¹ßÇö ½Ã½º..
iDimerize Inducible System..
[°³¿ä] Inducible Prote..
iDimerize Antibodies
Inducible Expression S..
Heterodimer System
Homodimer System
Regulated Transcriptio..
Reverse Dimerization S..
´Ü¹éÁú ¹ßÇö·® Á¶Àý ½Ã½ºÅÛ ..
ProteoTuner System
Tet-ProteoTuner Vector..
[°³¿ä] Yeast Hybrid System
[FAQ] Matchmaker Gold ..
[°³¿ä] Matchmaker Gold..
Yeast Two Hybrid System (Y..
Y2H Library Constructi..
Yeast Culture media (T..
Yeast Two-Hybrid (Y2H)..
Y2H Library (Yeast ³»..
Normalized Y2H Library..
Yeast Two hybrid vecto..
pBridge Vector
Yeast One-Hybrid (Y1H)
Yeast Culture Media (O..
Yeast One-Hybrid (Y1H)..
Matchmaker °ü·ÃÁ¦Ç°
Yeast Culture Media
Yeast Culture Media (..
Aureobasidin A
Yeast Plasmid Isolati..
Matchmaker Ç×ü
Insert Check Kit (prey..
Yeast Transformation M..
X-¥á-Gal
Yeast Transformation S..
Mammalian Cell Assay K..
Ư¡
°·ÂÇÏ°Ô ¹ßÇöÀ» Á¶Àý ÇÒ ¼ö ÀÖ´Â Tet-On 3G systemÀ» ±â¹ÝÀ¸·Î ÇÑ all-in-one vectorÇüÅÂ
³·Àº ¹é±×¶ó¿îµå¿Í ³ôÀº À¯µµ¹ßÇö È¿À²
10 ng/mlÀÇ doxycycline¿¡µµ ¹ÝÀÀÇÏ´Â ¹Î°¨µµ
Lenti-X Tet-One: °í¿ª°¡ÀÇ ·»Æ¼¹ÙÀÌ·¯½º¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Â ÆÐŰ¡ ½Ã½ºÅÛ Æ÷ÇÔ (Lenti-HTX Pakaging system)
Retro-X Tet-One: °í¿ª°¡ÀÇ ·¹Æ®·Î¹ÙÀÌ·¯½º¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Â ÆÐŰ¡ ½Ã½ºÅÛ Æ÷ÇÔ (Retro-X Universal Packaging System)
AAVpro Tet-One: helper-free packaging systemÀ» »ç¿ëÇϸç, AAV Particle ÃßÃâÀ» À§ÇÑ ÃßÃâ ½Ã¾à µîÀÌ Æ÷ÇԵǾîÀÖ´Ù.
Tet-One SystemÀÌ °³¹ßµÇ±â Àü¿¡´Â ClontechÀÇ Tet-on, Tet-off systemÀº response vector¿Í regulatory vector(doxycycline-responsive transactivator protein)ÀÎ 2°³ÀÇ vector¸¦ À¯ÀüÀÚ ¹ßÇöÀ» Á¶ÀýÇß´Ù.
Tet-One SystemÀº ÇϳªÀÇ º¤ÅÍ¿¡ ±¸¼º¿ä¼Ò¸¦ ¸ðµÎ Æ÷ÇÔÇÏ°í ÀÖ´Ù.
Tet-On 3G transactivator´Â human phosphoglycerate kinase 1 promoter¸¦ ÀÌ¿ëÇÏ¿© Á¤¹æÇâÀ¸·Î ¹ßÇöµÇ¸ç, ¹Ý´ë ¹æÇâ¿¡´Â
P
TRE3GS promoter¸¦ ÀÌ¿ëÇÏ¿© ¸ñÀû À¯ÀüÀÚ(Gene of interest)ÀÇ ¹ßÇöÀ» Á¶ÀýÇÑ´Ù. ±âÁ¸¿¡ ¹ßÇ¥µÈ all-in-one vector systemÀº 50~100¹è Á¤µµÀÇ À¯µµ¹ßÇö È¿À²À» º¸ÀδÙ. ÇÏÁö¸¸
ClontechÀÇ Tet-One systemÀº all-in-one vector ÇüÅ·ΠÃÖ´ë 2500¹èÀÇ À¯µµ¹ßÇö È¿À²À» º¸ÀδÙ.(Heinz
et al
, 2011).
±×¸². Tet-One System Mechanism
- The Tet-On 3G transactivator protein is expressed constitutively from the human PGK promoter
(P
PGK) but is unable to bind to the TRE3G promoter
(P
TRE3G) in the absence of doxycycline (Dox). When bound by Dox, supplied in the culture medium, the transactivator undergoes a conformational change, binds to
P
TRE3G and activates transcription of a transgene cloned downstream.
±×¸². Seven out of eight Tet-One clones show more than 1,000-fold induction of expression.
HeLa cells were infected with LVX-TetOne-Puro-Luc lentivirus (M.O.I. = 1) and eight individual clones were selected and expanded according to the protocol. Each clone was analyzed for doxycycline-induced expression of luciferase. Seven of the eight clones demonstrated more than 1,000-fold induced expression, including two clones with 5,000-fold induction, and one clone with 10,000-fold induction.
±×¸². The Tet-One system is highly sensitive to low levels of doxycycline (Dox).
HEK 293 cells were infected with LVX-TetOne-Luc lentivirus (M.O.I.=1) and the transduced pool was treated with 10-fold dilutions of doxycycline. 48 hr after treatment, the cells were harvested, lysed, and Western-blotted using anti-luciferase antibody. Luciferase expression reached maximum levels in the presence of only 10 ng/ml Dox.
References
Heinz, N., Schambach, A., Galla, M., Maetzig, T., Baum, C., Loew R., & Schiedlmeier, B. (2011) Retroviral and transposon-based Tet-regulated all-in-one vectors with reduced background expression and improved dynamic range.
Human Gene Ther
.
22
(2):166-176.
Keyword :
Tet À¯µµ ¹ßÇö ½Ã½ºÅÛ
[°³¿ä] Tet-on system
[¼±Åð¡À̵å] Tet Systems Product
Tet-On 3G À¯µµ ¹ßÇö ½Ã½ºÅÛ
Lenti-X Tet-On 3G Inducible Expression System
Tet-On 3G Cell Line
Tet Approved FBS
Tet °ü·Ã Ç×»ýÁ¦
Selection marker (Linear / Plasmid)
All-in-one vector ÇüÅÂÀÇ Tet-inducible syste..
Tet-One Inducible Expression (One vector System)
TetR Ç×ü
[Legacy] Tetracycline À¯µµ¹ßÇö ½Ã½ºÅÛ
Tet-Off Cell Lines
pTRE-Tight Vectors
pTet-tTS Vectors
IRES Tet À¯µµ¹ßÇö ½Ã½ºÅÛ