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Home > ÀüÁ¦Ç°º¸±â > Gene transfer (virus) > Mammalian Cell ¿ë Vector > ¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bidirectional Expression Vectors)
Bidirectional Æ÷À¯·ç ¼¼Æ÷ ¹ßÇö Vectors

¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bidirectional Expression Vectors)

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
631630
pBI-CMV1 Vector
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ¥ìg
1,035,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33278
Clontech
631631
pBI-CMV2 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
1,061,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33279
Clontech
631632
pBI-CMV3 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
1,061,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33280
Clontech
631633
pBI-CMV4 Vector
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 ¥ìg
1,061,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33281

  • Çü±¤ Çö¹Ì°æ ¶Ç´Â flow cytometry¸¦ ÀÌ¿ëÇÏ¿© transfection µÈ ¼¼Æ÷¸¦ ºü¸£°Ô È®ÀÎ
  • ´õ ºü¸£°í, ¾ÈÁ¤ÀûÀ¸·Î cloneÀ» ¼±º°Çϱâ À§ÇÑ bicistronic expression
  • ¸ñÀû ´Ü¹éÁúÀÎ untagged proteinÀÇ °í¹ßÇö
Bicistronic vector´Â ¸ñÀû À¯ÀüÀÚ¿Í fluorescent proteinÀÌ ¸ðµÎ ´ÜÀÏ mRNA¿¡¼­ ¹ø¿ªµÇµµ·Ï ÇÑ´Ù (Figure 1; 1, 2). °¢ vector´Â tagÀÌ ºÙ¾î ÀÖÁö ¾ÊÀº ¸ñÀû ´Ü¹éÁúÀ» °í¹ßÇöÇϵµ·Ï Á¦À۵Ǿú´Ù. µû¶ó¼­, Çü±¤À¸·Î Ç¥½ÄµÈ ¼¼Æ÷´Â °ÅÀÇ 100% °¡±îÀÌ ¸ñÀû À¯ÀüÀÚ¸¦ ¹ßÇöÇϰí, À̰ÍÀº flow cytometry³ª Çü±¤ Çö¹Ì°æÀ» »ç¿ëÇÏ¿© °£´ÜÇÏ°Ô ¼±º°ÇÏ¿© ¸ñÀû À¯ÀüÀÚ¸¦ ¹ßÇöÇÏ´Â ¼¼Æ÷¸¦ ºü¸£°Ô È®ÀÎÇÒ ¼ö ÀÖ´Ù.

IRES¿Í IRES2¿¡ ´ëÇÑ ´õ ÀÚ¼¼ÇÑ Á¤º¸´Â ȨÆäÀÌÁö (www.clontech.com)¿¡¼­ È®ÀÎÇϽʽÿÀ.


Figure 1. Translation of two proteins from a Living Colors pIRES2 Vector.
Bidirectional Reporter Vector
  • ÇϳªÀÇ ¸ñÀû ´Ü¹éÁú°ú ÇϳªÀÇ reporter ´Ü¹éÁúÀ» Ç×½ÃÀûÀ¸·Î ¹ßÇö
  • ³ì»öÀ̳ª Àû»ö ¶Ç´Â chemiluminescent reporter ¼±Åà °¡´É
Bidirectional vector´Â ºñ½ÁÇÑ ¼öÁØÀ¸·Î ¸ñÀû ´Ü¹éÁú°ú reporter ´Ü¹éÁú (¶Ç´Â 2 °³ÀÇ ¸ñÀû ´Ü¹éÁú)À» Ç×½ÃÀûÀ¸·Î ¹ßÇöÇÒ ¼ö ÀÖµµ·Ï Á¦À۵Ǿú´Ù. ¸ñÀû ´Ü¹éÁúÀÇ Ç×½ÃÀû ¹ßÇö½Ã½ºÅÛÀ» À§ÇÑ vector´Â ¾Æ·¡¿¡¼­ ¸ñÀû¿¡ ¸Â°Ô ¼±Åà °¡´ÉÇÏ´Ù.
  • 2 °³ÀÇ ¸ñÀû ´Ü¹éÁú - pBI-CMV1 (Figure 2)
  • Monomeric green fluorescent protein AcGFP1 - pBI-CMV2
  • ¾ÆÁÖ ¹àÀº green fluorescent protein ZsGreen1 - pBI-CMV3
  • Red fluorescent protein DsRed2 - pBI-CMV4
  • ºÐºñÇü chemiluminescent reporter, Metridia luciferase (MetLuc) pBICMV5 (Bidirectional Secreted Luciferase SystemÀÇ ±¸¼ºÇ°)
ÀÌ·¯ÇÑ bidirectional vector¸¦ »ç¿ëÇØ stable cloneÀ» ¸¸µé±â À§ÇÏ¿© linearized selection marker³ª antibiotic selection marker¸¦ Æ÷ÇÔÇϰí ÀÖ´Â vectorÀÇ cotransfectionÀ» ±ÇÀåÇÑ´Ù.
Inducible Bidirectional Vector ¶ÇÇÑ ÀÌ¿ë °¡´ÉÇϸç ÀÌ vector¿¡ ´ëÇÑ Á¤º¸´Â page 91¿¡¼­µµ È®ÀÎ °¡´ÉÇÏ´Ù.


Figure 2. pBI-CMV1 allows the constitutive expression of two proteins of interest. In the other pBI vectors, the ¡°Gene A¡± position is occupied by a reporter gene.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
References
1. Jackson, R. J. et al. (1990) Trends Biochem. Sci. 15(12):477-483.
2. Jang, S. K. et al. (1988) J. Virol. 62(8):2636-2643.

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