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Gene transfer (virus)
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Mammalian Cell ¿ë Vector
> ¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bidirectional Expression Vectors)
Bidirectional Æ÷À¯·ç ¼¼Æ÷ ¹ßÇö Vectors
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bidirectional Expression Vectors)
Á¦Á¶»ç
Á¦Ç°ÄÚµå
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(ºÎ°¡¼¼º°µµ)
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Clontech
631630
pBI-CMV1 Vector
20 ¥ìg
1,035,000¿ø
Clontech
631631
pBI-CMV2 Vector
20 ¥ìg
1,061,000¿ø
Clontech
631632
pBI-CMV3 Vector
20 ¥ìg
1,061,000¿ø
Clontech
631633
pBI-CMV4 Vector
20 ¥ìg
1,061,000¿ø
Product index
Adeno-Associated Virus (AA..
Overview - All about A..
Packaging (293T Cell L..
AAV1, 2, 5, 6 Vector S..
CRE Recombinase System
LacZ System
pAAV-ZsGreen1 Vector
CRISPR/Cas9 Vector Sys..
CRISPR/SaCas9 Vector S..
Tet-Inducible System (..
Purification Kit Maxi/..
Purification Kit (AAV2..
AAVpro¢ç Concentrator
Titration Kit (qPCR)
Extraction Solution
Lentivirus
Lenti-X ¼±Åð¡À̵å
[°³¿ä] Packaging Singl..
[FAQ] Lentiviral Gene ..
CMV promoter º¤ÅÍ ½Ã½º..
EF1 ¥á promoter º¤ÅÍ ..
Lenti-X¢â SARS-CoV-2 P..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tet-on inducible Expre..
Tet One Inducible Expr..
(Legacy) Tet-Inducible..
Reporter assay ½Ã½ºÅÛ ..
Packaging single shots
Packaging Cell Lines
Á¤È®ÇÑ ¿ª°¡ ÃøÁ¤
GoStix¢â Plus
Lentivirus ³óÃà ½Ã¾à
Lentivirus Á¤Á¦
Ecotropic Receptor Boo..
Transduction ½Ã¾à
Provirus Quantitation ..
Integration Site Analy..
TransIT¢ç Lentivirus S..
Retrovirus
Retro-X ¼±Åà °¡À̵å
Packaging Cell £¦ ½Ã½º..
Retrovirus vector £¦ ..
Çü±¤´Ü¹éÁú Retrovirus ..
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
pDON-5 DNA
pDON-AI-2 DNA
pMEI-5 DNA
iPS Cell Generation¢â ..
Retrovirus Packaging K..
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (T..
Tet One Inducible Syst..
qRT-PCR titer ÃøÁ¤
Titer Set (for Real Ti..
Retrovirus ³óÃà
RetroNectin¢ç (Recombi..
Receptor Boosters (Eco..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analy..
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression ..
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi ..
Adenovirus Á¤Á¦ (Mega ..
qPCR titer ÃøÁ¤ Kit
Hexon ±â¹Ý titer ÃøÁ¤
Adeno-X GoStix
CAR Receptor Booster
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bi..
EF1¥á Promotor Vector ..
epitope tag (HA or c-M..
in vivo Vector, pLIVE ..
Magnetic Myc IP Kit
pBApo-CMVvector ½Ã¸®Áî
pBApo-EF1¥á Vector ½Ã..
pIRES Bicistronic Expr..
Çü±¤ Çö¹Ì°æ ¶Ç´Â flow cytometry¸¦ ÀÌ¿ëÇÏ¿© transfection µÈ ¼¼Æ÷¸¦ ºü¸£°Ô È®ÀÎ
´õ ºü¸£°í, ¾ÈÁ¤ÀûÀ¸·Î cloneÀ» ¼±º°Çϱâ À§ÇÑ bicistronic expression
¸ñÀû ´Ü¹éÁúÀÎ untagged proteinÀÇ °í¹ßÇö
Bicistronic vector´Â ¸ñÀû À¯ÀüÀÚ¿Í fluorescent proteinÀÌ ¸ðµÎ ´ÜÀÏ mRNA¿¡¼ ¹ø¿ªµÇµµ·Ï ÇÑ´Ù (Figure 1; 1, 2). °¢ vector´Â tagÀÌ ºÙ¾î ÀÖÁö ¾ÊÀº ¸ñÀû ´Ü¹éÁúÀ» °í¹ßÇöÇϵµ·Ï Á¦À۵Ǿú´Ù. µû¶ó¼, Çü±¤À¸·Î Ç¥½ÄµÈ ¼¼Æ÷´Â °ÅÀÇ 100% °¡±îÀÌ ¸ñÀû À¯ÀüÀÚ¸¦ ¹ßÇöÇϰí, À̰ÍÀº flow cytometry³ª Çü±¤ Çö¹Ì°æÀ» »ç¿ëÇÏ¿© °£´ÜÇÏ°Ô ¼±º°ÇÏ¿© ¸ñÀû À¯ÀüÀÚ¸¦ ¹ßÇöÇÏ´Â ¼¼Æ÷¸¦ ºü¸£°Ô È®ÀÎÇÒ ¼ö ÀÖ´Ù.
IRES¿Í IRES2¿¡ ´ëÇÑ ´õ ÀÚ¼¼ÇÑ Á¤º¸´Â ȨÆäÀÌÁö (www.clontech.com)¿¡¼ È®ÀÎÇϽʽÿÀ.
Figure 1. Translation of two proteins from a Living Colors pIRES2 Vector.
Bidirectional Reporter Vector
ÇϳªÀÇ ¸ñÀû ´Ü¹éÁú°ú ÇϳªÀÇ reporter ´Ü¹éÁúÀ» Ç×½ÃÀûÀ¸·Î ¹ßÇö
³ì»öÀ̳ª Àû»ö ¶Ç´Â chemiluminescent reporter ¼±Åà °¡´É
Bidirectional vector´Â ºñ½ÁÇÑ ¼öÁØÀ¸·Î ¸ñÀû ´Ü¹éÁú°ú reporter ´Ü¹éÁú (¶Ç´Â 2 °³ÀÇ ¸ñÀû ´Ü¹éÁú)À» Ç×½ÃÀûÀ¸·Î ¹ßÇöÇÒ ¼ö ÀÖµµ·Ï Á¦À۵Ǿú´Ù. ¸ñÀû ´Ü¹éÁúÀÇ Ç×½ÃÀû ¹ßÇö½Ã½ºÅÛÀ» À§ÇÑ vector´Â ¾Æ·¡¿¡¼ ¸ñÀû¿¡ ¸Â°Ô ¼±Åà °¡´ÉÇÏ´Ù.
2 °³ÀÇ ¸ñÀû ´Ü¹éÁú - pBI-CMV1 (Figure 2)
Monomeric green fluorescent protein AcGFP1 - pBI-CMV2
¾ÆÁÖ ¹àÀº green fluorescent protein ZsGreen1 - pBI-CMV3
Red fluorescent protein DsRed2 - pBI-CMV4
ºÐºñÇü chemiluminescent reporter, Metridia luciferase (MetLuc) pBICMV5 (Bidirectional Secreted Luciferase SystemÀÇ ±¸¼ºÇ°)
ÀÌ·¯ÇÑ bidirectional vector¸¦ »ç¿ëÇØ stable cloneÀ» ¸¸µé±â À§ÇÏ¿© linearized selection marker³ª antibiotic selection marker¸¦ Æ÷ÇÔÇϰí ÀÖ´Â vectorÀÇ cotransfectionÀ» ±ÇÀåÇÑ´Ù.
Inducible Bidirectional Vector ¶ÇÇÑ ÀÌ¿ë °¡´ÉÇϸç ÀÌ vector¿¡ ´ëÇÑ Á¤º¸´Â page 91¿¡¼µµ È®ÀÎ °¡´ÉÇÏ´Ù.
Figure 2. pBI-CMV1 allows the constitutive expression of two proteins of interest.
In the other pBI vectors, the ¡°Gene A¡± position is occupied by a reporter gene.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
References
1. Jackson, R. J. et al. (1990) Trends Biochem. Sci. 15(12):477-483.
2. Jang, S. K. et al. (1988) J. Virol. 62(8):2636-2643.
Keyword :
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾·ùÀÇ tag¸¦ ÀÌ¿ëÇÑ ´Ü¹éÁú »ý»ê ¹× Á¤Á¦
Bidirectional Æ÷À¯·ç ¼¼Æ÷ ¹ßÇö Vectors
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bidirectional Expression Vectors)
EF1¥á Promotor Vector (Çü±¤ ´Ü¹éÁú, IRES ±âŸ)
epitope tag (HA or c-Myc) Vectors
in vivo Vector, pLIVE Vector ½Ã¸®Áî
Magnetic Myc IP Kit
Æ÷À¯µ¿¹° ¼¼Æ÷ ¹ßÇö Vector
pBApo-CMVvector ½Ã¸®Áî
Æ÷À¯µ¿¹° ¼¼Æ÷ ¹ßÇö Vector
pBApo-EF1¥á Vector ½Ã¸®Áî
pIRES Bicistronic Expression Vector