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Home
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NGS °ü·Ã
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ChIP-Seq / Meth-Seq
> Meth-Seq DNA Enrichment Kit
¸ÞÆ¿È DNA ¼¿ ºÐ¼®À» À§ÇÑ NGS Prep kit (Epigenetics)
Meth-Seq DNA Enrichment Kit
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Clontech
635023
EpiXplore¢â Meth-Seq DNA Enrichment Kit
Each
1,664,000¿ø
2,080,000¿ø
05.02 ~ 06.30
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Rapid, user-friendly protocol which separates methylated DNA from unmethylated DNA
Ligation-independent sequencing adapter addition for library preparation
DNA inputs of 25 ng - 1 ¥ìg sheared genomic DNA
Generate Illumina
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-ready sequencing libraries from the enriched DNA in ~4 hour
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EpiXplore¢â Meth-Seq DNA Enrichment KitÀº sheared genomic DNA·ÎºÎÅÍ ¸ÞÆ¿ÈµÈ DNA¿Í ºñ¸ÞÆ¿ÈµÈ DNA ÀýÆíÀ» ºÐ¸®ÇÏ°í ±×·ÎºÎÅÍ Illumina
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sequencing library¸¦ Á¦ÀÛ °¡´ÉÇÑ ½ÇÇè¿¡ ÇÊ¿äÇÑ ¸ðµç ±¸¼ºÇ°ÀÌ Æ÷ÇÔµÈ complete systemÀÌ´Ù.
EpiXplore¢â Meth-Seq DNA Enrichment Kit¸¦ ÀÌ¿ëÇϸé Clontech µ¶ÀÚÀÇ
Capturem Nanoprep column
±â¼ú°ú His-tagged MBD2 proteinÀ» Á¶ÇÕÇÑ DNA Enrichment±â¼úÀ» ÀÌ¿ëÇÏ¿© ¸ÞÆ¿È ¹× ºñ¸ÞÆ¿È DNA ºÐ¸®¸¦ 2½Ã°£ À̳»¿¡ ÁøÇàÇÒ ¼ö ÀÖ´Ù (±×¸² 1). ÀÌÈÄ, DNA SMART
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±â¼úÀ» ÀÌ¿ëÇÏ¿© ¸ÞÆ¿È DNA ÀýÆí (¶Ç´Â, ºñ¸ÞÆ¿È DNA ÀýÆí)À¸·ÎºÎÅÍ Illumina
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compatible sequencing library¸¦ Á¦ÀÛÇÑ´Ù (±×¸² 2). NGS Library PrepÀº ligation-independent DNA SMART
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¹æ¹ýÀ¸·Î DNA ÀýÆíÀÇ ¾ç ¸»´Ü¿¡ Illumina
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-specific sequencing adaptor¸¦ ºÎ°¡ÇÑ´Ù. º» ¹æ¹ýÀ» ÀÌ¿ëÇϸé low-input DNA »ùÇ÷κÎÅÍ 4½Ã°£ ³»¿¡ Sequencing library¸¦ Á¦ÀÛ ÇÒ ¼ö ÀÖ´Ù.
±×¸² 1. Flowchart for separating methylated and unmethylated fractions of DNA.
In the enrichment portion of the protocol, sheared DNA is incubated with his-tagged MBD2 protein for 1 hr, which allows the methylated DNA fraction to bind to the protein. The solution is then added to the Capturem nanoprep column, which binds the his-tagged protein and thus the methylated DNA. Subsequent washes with the Unmethylated Elution Buffer remove the unmethylated DNA. The methylated DNA is then eluted in a high-salt buffer. After cleanup, this enriched DNA can be used as the input for library preparation.
±×¸² 2. Flowchart for DNA-SMART ChIP-Seq technology.
This single-tube workflow allows users to generate Illumina-compatible libraries for meth-seq experiments. After library size selection and purification, the total time from input DNA to meth-seq library is approximately four hours. DNA SMART technology eliminates the need for an adapter ligation step and associated cleanup, reducing the loss of limited input DNA.
±×¸² 3. Distinct methylation patterns are observed in aligned NGS data of libraries generated from methylated and unmethylated DNA fractions.
A representative region of Arabidopsis chromosome 3 (outlined in the green box) shows the peaks identified in the DNA enriched for methylated and unmethylated fractions. The methylated regions are outlined in red boxes and the unmethylated regions are outlined in blue boxes. The amount of initial input DNA is shown along the left-hand side of the alignments. The data show good concordance with the publicly available bisulfite sequencing data. Data were visualized using the Integrative Genomics Viewer (Broad Institute). Public data was obtained from GEO accession GSM980986; Stroud et al., 2013.
Application
- Sequencing library preparation for methylated DNA-seq from low-input, sheared genomic DNA
- Illumina
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-specific NGS sequencing library production
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ ÂüÁ¶Çϼ¼¿ä)
- EpiXplore Meth-Seq DNA Enrichment Components
- SeqAmp¢â DNA Polymerase
- DNA SMART ChIP-Seq Components
- Indexing Primer Set HT for Illumina - 12
Keyword : Methylation,Methylated DNA-Seq,NGS,DNA-Seq,SMART,SMARTer,Epigenetics,EpiXplore
ChIP-Seq / Meth-Seq
[Àû¿ë] ThruPLEX¸¦ ÀÌ¿ëÇÑ ChIP-seq
[³í¹®] ThruPLEX¢ç for ChIP-Seq
50pg ChIP DNA-seq Kit
DNA SMART ChIP-Seq Kit
¸ÞÆ¿È DNA ¼¿ ºÐ¼®À» À§ÇÑ NGS Prep kit (Ep..
Meth-Seq DNA Enrichment Kit
Micrococcal Nuclease