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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > Stranded RNA-seq > [New]Mid Input Stranded RNA-Seq Kit
Mid Input Strand- Specific Total RNA-Seq

[New]Mid Input Stranded RNA-Seq Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Clontech
635049
SMART-Seq¢ç Total RNA Mid Input
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
24ȸ
(Package)
2,085,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x346312
Clontech
635050
SMART-Seq¢ç Total RNA Mid Input
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
96ȸ
(Package)
6,132,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
635048
SMART-Seq¢ç Total RNA Mid Input
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
384ȸ (635050x4)
22,076,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

¡Ø º¯°æ»çÇ×
i. º» Á¦Ç°Àº ±âÁ¸ SMARTer¢ç Stranded RNA-Seq Kit (Code 634836), SMARTer¢ç Stranded Total RNA Sample Prep Kit - Low Input Mammalian (Code 634861), SMARTer¢ç Stranded RNA-Seq Kit HT (Code 634862)ÀÇ ¸®´º¾ó Á¦Ç°ÀÔ´Ï´Ù.
ii. Á¦Ç°»ç¿ë À¯¿¬¼ºÀ» À§ÇØ Á¦Ç° ±¸¼º¿¡¼­ index°¡ Á¦¿ÜµÇ¾ú½À´Ï´Ù. ÇÊ¿ä½Ã º°µµ ±¸¸Å ¹Ù¶ø´Ï´Ù.
>> ȣȯµÇ´Â Unique Dual Index kits (UDI kits)
iii. rRNA Á¦°Å¿ë RiboGone Mammalian (Code 634846)Àº º»Á¦Ç°¿¡ Æ÷ÇԵǾî ÀÖÁö ¾ÊÁö¸¸ mammalian total RNA¿¡ »ç¿ë½Ã º°µµ ±¸¸ÅÇÒ ¼ö ÀÖ½À´Ï´Ù.

ÀÚ¼¼ÇÑ ³»¿ëÀº ´ÙÀ½ ¾È³»ÆäÀÌÁö¸¦ Âü°íÇϼ¼¿ä! Á¦Ç° ¼±ÅÃÀÌ ½¬¿öÁý´Ï´Ù.
>> RNA-Seq Á¦Ç°¸íÀÌ ÀÌ·¸°Ô ¹Ù²ò´Ï´Ù

  • ³ôÀº Á¤È®µµ: 99% ÀÌ»óÀÇ Á¤È®µµ·Î °¢ Àü»çüÀÇ origin °¡´Ú ½Äº° °¡´É
  • ³ôÀº °¨µµ: 100 pgÀÇ ÀûÀº RNA input¿¡ ÀÖ´Â ¼Ò·®ÀÇ Àü»çüµµ °ËÃâÀÌ °¡´É. rRNA¸¦ Á¦°ÅÇÑ RNA ¶Ç´Â poly(A)-purified RNA¸¦ »ç¿ëÇÏ´Â °ÍÀ» ±ÇÀåÇÔ.
  • Illumina-ready library: PCR ÁõÆø ´Ü°è¿¡¼­ Illumina index¿Í adaptor¸¦ Ãß°¡
  • °¡Àå °£´ÜÇÑ ¿öÅ©Ç÷οì Á¦°ø: ´Ü 4½Ã°£ ¸¸¿¡ °£´ÜÇÏ°Ô library Á¦ÀÛ
Á¦Ç° ¼³¸í
º» Á¦Ç°Àº 99% ÀÌ»óÀÇ Á¤È®µµ·Î strand ¹æÇ⼺ Á¤º¸¸¦ À¯ÁöÇÏ´Â Illumina-ready library¸¦ ÁغñÇϱâ À§ÇÑ ¼Ö·ç¼ÇÀ» Á¦°øÇϸç, rRNA-depleted sample ¶Ç´Â poly(A)-enriched sample¿¡ ÃßõÇÑ´Ù. ¶ÇÇÑ eukaryotic ¹× prokaryotic RNA »ùÇÿ¡ ȣȯµÇ´Â random-priming ¹æ¹ýÀ» »ç¿ëÇϸç, 100 pg - 100 ng RNA inputÀ¸·Î °íÇ°Áú µ¥ÀÌÅ͸¦ »ý¼ºÇÑ´Ù.
ÀÌ Å°Æ®´Â coding, noncoding RNA Àüü¿¡ ´ëÇÑ Àü»çü Á¤º¸¸¦ Á¦°øÇϸç, ¿öÅ©ÇÃ·Î¿ì ¾È¿¡ Illumina index ¹× adapter¸¦ Ãß°¡ÇÏ´Â °úÁ¤ÀÌ Æ÷ÇÔµÇ¾î ¸Å¿ì °£ÆíÇÏ°Ô library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Ù.
¶ÇÇÑ Unique Dual Index Kits (Code 634752-634756)¸¦ »ç¿ëÇϸé ÃÖ´ë 384°³ÀÇ Illumina-ready library¸¦ multiplexingÇÒ ¼ö ÀÖ´Ù.
rRNA Á¦°Å°¡ ÇÊ¿äÇÑ °æ¿ì, 10 - 100 ng total RNA input¿¡ Àû¿ë°¡´ÉÇÑ RiboGone¢â- Mammalian kit¸¦ ÃßõÇÑ´Ù.

*±âŸ input amount¿¡ µû¸¥ Ãßõ Å°Æ®:
- Mammalian total RNA 250 pg-10 ng : SMARTer¢ç Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Code 634485 ¿Ü)
- Mammalian total RNA 100 ng-1 ¥ìg : SMART-Seq Total RNA High Input (RiboGone Mammalian) (Code 635046)
Àû¿ë
  • Illumina Ç÷§ÆûÀ» ÀÌ¿ëÇÑ prokaryotic ¶Ç´Â mammalian »ùÇÃÀÇ RNA-Seq
  • Strand ¹æÇ⼺ Á¤º¸¸¦ À¯ÁöÇÏ´Â NGS library Áغñ
  • Coding ¹× noncoding ¼­¿­ Á¤º¸ ºÐ¼®
ÀÚ¼¼ÇÑ ±¸¼ºÀº CoA¸¦ Âü°íÇϼ¼¿ä.
RNA-Seq ·¯´×¼¾Å͸¦ ÅëÇØ º» Á¦Ç°¿¡ ´ëÇÑ ´õ ¸¹Àº µ¥ÀÌÅÍ¿Í FAQ¸¦ Âü°íÇϼ¼¿ä.

Keyword : BioMarker,FFPE,Cell-free RNA,Degraded RNA,Non-coding RNA,ncRNA,lncRNA,Cancer,Transcriptomics,transcriptome,LCM,stranded-seq,smarter,random primer,smart-seq,bacteria,metatranscriptomics

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