´Ý±â
TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Å°¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø 
  • ¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢support@takara.co.kr
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢tkbd@takara.co.kr
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > mRNA-Seq (Single cell) > Ultra low input mRNA-Seq Kit
¼ö¸¹Àº CitationÀÌ Áõ¸íÇÏ´Â ±Ø¼Ò·® »ùÇà ºÐ¼® ÃÖ°­ÀÚ

Ultra low input mRNA-Seq Kit

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
634888
SMART-Seq¢ç v4 Ultra¢ç Low Input RNA Kit for Sequencing
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
12ȸ
(Package)
°¡°Ý¹®ÀÇ   Àç°í ¼ÒÁø ÈÄ Á¾¸Å Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33067
Clontech
634889
SMART-Seq¢ç v4 Ultra¢ç Low Input RNA Kit for Sequencing
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
24ȸ
(Package)
°¡°Ý¹®ÀÇ   Àç°í ¼ÒÁø ÈÄ Á¾¸Å Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
634890
SMART-Seq¢ç v4 Ultra¢ç Low Input RNA Kit for Sequencing
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
48ȸ
(Package)
°¡°Ý¹®ÀÇ   Àç°í ¼ÒÁø ÈÄ Á¾¸Å Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
635013
10X Lysis Buffer
°ü·ÃÇмú ±¸¸ÅÇϱâ
1.85 ml
73,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33063, x33067




* SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing Á¦Ç°°ú SMART-Seq® v4 PLUS Kit Á¦Ç°Àº °¢°¢ SMART-Seq® mRNA, SMART-Seq® mRNA LP·Î ¸®´º¾ó µÇ¾ú½À´Ï´Ù.
* UMIs ºÎ°¡·Î Á¤È®µµ¿Í ½Å·Úµµ¸¦ ³ôÀÎ ½ÅÁ¦Ç°, SMART-Seq® mRNA LP (with UMIs)°¡ Ãâ½ÃµÇ¾ú½À´Ï´Ù.

* PLUS kit´Â »ùÇ÷κÎÅÍ lllumina¢ç ºÐ¼®¿ë library Á¦ÀÛÀ» À§ÇÑ, library preparation kit¿Í single-use unique dual index (UDI)¸¦ Æ÷ÇÔÇÏ°í ÀÖ½À´Ï´Ù.

* ÀÚµ¿È­ ½Ã½ºÅÛ (Apollo¢â Library Prep System (½Ã½ºÅÛÀº ÆǸÅÁ¾·á))¿¡ Àû¿ë°¡´ÉÇÑ ½Ã¾à
SMART-Seq¢ç v4 Reagent Kit for the SMARTer¢â Apollo¢â System (Code 640170)

[°ü·Ã Á¤º¸ÀÚ·á]
SMART-Seq v4 Single cell RNA-seq ¿Â¶óÀÎ ¼¼¹Ì³ª ¹Ù·Îº¸±â
SMART-Seq v4 Ultra Low Input Kit¸¦ ÀÌ¿ëÇÑ Single cell RNA-Seq ³í¹® ¸®½ºÆ® º¸±â

  • 10 pg ¼öÁØÀÇ ±Ø¼Ò·® total RNA¿¡µµ Àû¿ë °¡´É
  • Unparalleled sensitivity and reproducibility
  • Single-tube workflows - cDNA ÇÕ¼ººÎÅÍ library Á¦ÀÛ °úÁ¤À» °¢°¢ single tube¿¡¼­ ÁøÇàÇÒ ¼ö ÀÖ¾î, »ùÇà ¼Õ½ÇÀ̳ª ¿À¿° µî ½ÇÇè °úÁ¤¿¡¼­ ¹ß»ýÇÒ ¼ö ÀÖ´Â ¿À·ù ÃÖ¼ÒÈ­
  • High-quality RNA-seq data - total RNA·ÎºÎÅÍ °¡Àå ¸¹Àº ¼öÀÇ À¯ÀüÀÚ ºÐ¼®
  • Increase your sequencing power - ¸¹Àº »ùÇÃÀ» ºÐ¼®ÇÒ ¼ö ÀÖ´Â Unique dual index (UDI) »ç¿ë
  • High library yield - ³ôÀº yieldÀÇ library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ¾î, NovaSeq¢â system°ú °°Àº high-throughput sequencer¿¡ Àû¿ëÇϰųª, ¿©·¯ Â÷·Ê ºÐ¼® °¡´É
Á¦Ç°¼³¸í
SMART-Seq¢ç v4 Ultra¢ç Low Input RNA Kit for Sequencing (SSv4)¿Í SMART-Seq¢ç v4 PLUS kit (SSv4 PLUS)´Â ±Ø¼Ò·® (Ultra-Low input) »ùÇÃÀ» À§ÇÑ SMARTer Ultra Low kit ½Ã¸®ÁîÀÇ 4¼¼´ë Á¦Ç°À¸·Î, ±Ø¼Ò·® »ùÇÃÀ» °¡Àå ¹Î°¨µµ ÀÖ°Ô ºÐ¼®ÇÒ ¼ö ÀÖ´Ù. SSv4 PLUS Á¦Ç°À» ÀÌ¿ëÇϸé, 1,000°³ ÀÌÇÏÀÇ intactÇÑ ¼¼Æ÷ ȤÀº 10 pg - 10 ngÀÇ high quality RNA (RIN > 8)·ÎºÎÅÍ oligo(dT) primer¸¦ ÀÌ¿ëÇØ °íÇ°Áú full-length cDNA¸¦ Á¦Á¶ÇÑ ÈÄ, lllumina¢ç ºÐ¼®¿ë library Á¦ÀÛ±îÁö ÁøÇàÇÒ ¼ö ÀÖ´Ù.
º» Á¦Ç°Àº SMART (Switching Mechanism at 5' End of RNA Template) ±â¼ú¿¡ Locked Nucleic Acid (LNA) ±â¼úÀ» Á¢¸ñÇÔÀ¸·Î½á template switching È¿À²À» ´õ¿í Çâ»ó½ÃÄ×´Ù. µû¶ó¼­, ÀÌÀü ¼¼´ëÀÇ Á¦Ç°º¸´Ù ´õ¿í ¸¹Àº ¼öÀÇ À¯ÀüÀÚ¸¦ °ËÃâÇÒ ¼ö ÀÖ¾î Àü¹ÝÀûÀÎ Àü»çü ¿¬±¸ (full transcriptome analysis)¿¡ ÀÖ¾î ¸Å¿ì À¯¿ëÇÏ´Ù. SSv4´Â ³ôÀº ÀçÇö¼º°ú gene-body coverage »Ó ¾Æ´Ï¶ó GC-rich Àü»çüµµ Á¤È®ÇÏ°Ô cDNA·Î ÇÕ¼ºÇÒ ¼ö ÀÖ´Ù.
SMART-Seq¢ç v4 PLUS kit (SSv4 PLUS)¿¡ Æ÷ÇԵǾî ÀÖ´Â library preparation kit´Â enzymatic fragmentation¿Í stem-loop adaptor¸¦ ÇÔ²² »ç¿ëÇÏ¿©, 2½Ã°£ ³» single tube¿¡¼­ purification °úÁ¤ ¾øÀÌ cDNA·ÎºÎÅÍ library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Ù. µû¶ó¼­ »ùÇÃÀÇ ¼Õ½ÇÀ̳ª ¿À¿° µî ½ÇÇè °úÁ¤¿¡¼­ ¹ß»ýÇÒ ¼ö ÀÖ´Â ¿À·ù¸¦ ÃÖ¼ÒÈ­ ÇÒ ¼ö ÀÖ´Ù. ÀÌ °úÁ¤À» ÅëÇØ Á¦ÀÛµÈ library´Â SMART-Seq2 µî ±âÁ¸ÀÇ ¹æ¹ý ¹× ´ç»ç ±âÁ¸ Á¦Ç° (SMART-Seq¢ç v3)¿¡ ºñÇØ ÀçÇö¼ºÀÌ ³ô°í, ÈξÀ ¿ì¼öÇÒ »Ó ¾Æ´Ï¶ó, ³ôÀº yield·Î Á¦À۵Ǿî high-throughput sequencer¿¡ Àû¿ëÇϰųª, ¿©·¯ Â÷·Ê ºÐ¼®ÇÒ ¼ö ÀÖ´Ù.


±×¸² 1. SMART±â¼úÀ» ÀÌ¿ëÇÑ cDNA ÇÕ¼º °úÁ¤

The SMART-Seq¢ç v4 Oligonucleotide, 3¡¯ SMART-Seq¢ç CDS Primer II A, and PCR Primer II A all contain a stretch of identical sequence. The black star indicates a chemical block on the 5¡¯ end of the oligonucleotide.


±×¸² 2. SSv4¸¦ ÀÌ¿ëÇßÀ» ¶§ ³ôÀº ¹Î°¨µµ¿Í mapping È¿À²

Replicate libraries were generated from 10 pg Mouse Brain Total RNA using the SMART-Seq¢ç v4 kit, the SMARTer¢ç Ultra Low v3 kit, or the SMART-Seq2 method. 18 PCR cycles were used to amplify cDNA libraries with the SMART-Seq2 method and SMARTer¢ç Ultra Low v3 kit; however, only 17 PCR cycles were needed for the SMART-Seq¢ç v4 libraries.


±×¸² 3. ±âÁ¸ ¹æ¹ýÀÎ SMART-Seq2, SSv3¿Í ºñ±³ÇßÀ» ¶§, SSv4ÀÇ ³ôÀº gene coverage

Gene body coverage shown is the average of two replicate libraries prepared from 10 pg Mouse Brain Total RNA using the three different cDNA synthesis methods. The SMARTer¢ç Ultra Low v3 kit produced a slight 3¡Ç bias, and the SMART-Seq¢ç v4 kit produced a slight 5¡Ç bias; however, the overall coverage was fairly even.


±×¸² 4. ³·Àº inputÀÇ »ùÇà »ç¿ë¿¡µµ ³ôÀº ÀçÇö¼º

FPKMs from replicate libraries generated from 10 pg of Mouse Brain Total RNA using the SMART-Seq¢ç v4 kit, the SMARTer¢ç Ultra Low v3 kit method were compared. For transcripts with FPKM <100, the correlation between replicates was much higher for the SMART-Seq¢ç v4 kit (Pearson R = 0.739; Panel B) compared to the SMARTer Ultra Low v3 (Pearson R = 0.376; Panel A). For all transcripts (shown in the scatterplots on the right) the correlation between replicates was high for each of the three methods (Pearson R between 0.911-0.972), though the SMART-Seq¢ç v4 kit did have the highest correlation. Transcripts represented in only one replicate can be seen along the X- and Y-axes of the scatter plots showing all transcripts.


±×¸² 5.SSv4-HT PLUS¸¦ ÀÌ¿ëÇÑ library¿¡¼­ Nextera¢ç XTº¸´Ù ´õ ³ôÀº yield¿Í À¯ÀüÀÚ °ËÃâ

(Panel A) SSv4 chemistry was used to produce cDNA, in triplicate, from 10 pg of Mouse Brain Total RNA. Illumina-compatible libraries were then generated using the SMART-Seq¢ç Library Prep Kit (not sold separately) portion of the SSv4 PLUS kit or Nextera¢ç XT and sequenced on a NextSeq 500. The reads were normalized to 4M paired-end reads and analyzed as described in the techical note.
(Panel B) The library yield obtained with the SSv4 PLUS kit is higher than the yield obtained when using Nextera¢ç XT.
(Panel C) The number of genes identified was also higher for the SSv4 PLUS kit compared to Nextera¢ç XT.
Àû¿ë
±Ø¼Ò·® »ùÇ÷κÎÅÍ full-length transcriptome ºÐ¼® (transcript isoforms, gene fusions, point mutations µî)À» À§ÇØ, cDNA ÇÕ¼º °úÁ¤¿¡¼­ »ç¿ëÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°À» ÀÌ¿ëÇØ ÇÕ¼ºÇÑ cDNA´Â SMART-Seq Library Prep kit (SSv4 PLUS kit ³» Æ÷ÇÔ, º°µµ ±¸¸Å ºÒ°¡) ¸¦ ÀÌ¿ëÇÏ¿© Illumina¢ç ºÐ¼®¿ë sequencing library·Î Á¦ÀÛÇÒ ¼ö ÀÖ´Ù. ȤÀº Ion Xpress Plus Fragment Library Kit¸¦ ÀÌ¿ëÇÏ¿©, Ion Torrent sequencing¸¦ ÁøÇàÇÒ ¼ö ÀÖ´Ù.
ÀÚ¼¼ÇÑ ±¸¼ºÀº CoA¸¦ Âü°íÇϼ¼¿ä.
Citation
Multi-omic analyses of changes in the tumor microenvironment of pancreatic adenocarcinoma following neoadjuvant treatment with anti-PD-1 therapy
Cancer cell    |    2022 Oct 27    |    PubMed ID: 36306792    |    Read Article

PD-1 -cis IL-2R agonism yields better effectors from stem-like CD8 + T cells
Nature    |    2022 Sep 28    |    PubMed ID: 36171284    |    Read Article

An amygdala circuit that suppresses social engagement
Nature    |    2022 Jul 04    |    PubMed ID: 33790466    |    Read Article

An engineered IL-2 partial agonist promotes CD8 + T cell stemness
Nature    |    2022 Jun 07    |    PubMed ID: 34526724    |    Read Article

The spatial landscape of progression and immunoediting in primary melanoma at single cell resolution
Cancer discovery    |    2022 Jun 02    |    PubMed ID: 35404441    |    Read Article

MCM complexes are barriers that restrict cohesin-mediated loop extrusion
Nature    |    2022 May 18    |    PubMed ID: 35585235    |    Read Article

Extricating human tumour immune alterations from tissue inflammation
Nature    |    2022 May 11    |    PubMed ID: 35545675    |    Read Article

Anatomic position determines oncogenic specificity in melanoma
Nature    |    2022 Apr 01    |    PubMed ID: 35355015    |    Read Article

Corticosterone inhibits Gas6 to govern hair follicle stem cell quiescence
Nature    |    2022 Mar 15    |    PubMed ID: 33790465    |    Read Article

An autoimmune stem-like CD8 T cell population drives type 1 diabetes
Nature    |    2022 Feb 01    |    PubMed ID: 34847567    |    Read Article

Tissue-resident macrophages provide a pro-tumorigenic niche to early NSCLC cells.
Nature    |    2022 Jan 11    |    PubMed ID: 34135508    |    Read Article

Exercise plasma boosts memory and dampens brain inflammation via clusterin
Nature    |    2021 Dec 08    |    PubMed ID: 34880498    |    Read Article

Fate mapping of single NK cells identifies a type 1 innate lymphoid-like lineage that bridges innate and adaptive recognition of viral infection
Immunity    |    2021 Oct 12    |    PubMed ID: 34437840    |    Read Article

Human neocortical expansion involves glutamatergic neuron diversification
Nature    |    2021 Oct 06    |    PubMed ID: 34616067    |    Read Article

A transcriptomic and epigenomic cell atlas of the mouse primary motor cortex
Nature    |    2021 Oct 06    |    PubMed ID: 34616066    |    Read Article

Comparative cellular analysis of motor cortex in human, marmoset and mouse
Nature    |    2021 Oct 06    |    PubMed ID: 34616062    |    Read Article

Regulation of Prefrontal Patterning and Connectivity by Retinoic Acid
Nature    |    2021 Oct 01    |    PubMed ID: 34599305    |    Read Article

Distinct developmental pathways from blood monocytes generate human lung macrophage diversity.
Immunity    |    2021 Sep 07    |    PubMed ID: 33382972    |    Read Article

Adult neural stem cell activation in mice is regulated by the day/night cycle and intracellular calcium dynamics.
Cell    |    2021 Aug 24    |    PubMed ID: 33482084    |    Read Article

Genetic insights into biological mechanisms governing human ovarian ageing
Nature    |    2021 Aug 01    |    PubMed ID: 34349265    |    Read Article

The Identity of Human Tissue-Emigrant CD8 + T Cells.
Cell    |    2021 May 18    |    PubMed ID: 33306960    |    Read Article

Notch4 signaling limits regulatory T-cell-mediated tissue repair and promotes severe lung inflammation in viral infections
Immunity    |    2021 Apr 28    |    PubMed ID: 33915108    |    Read Article

Impaired Kupffer Cell Self-Renewal Alters the Liver Response to Lipid Overload during Non-alcoholic Steatohepatitis.
Immunity    |    2021 Apr 22    |    PubMed ID: 32562600    |    Read Article

H2AK119ub1 guides maternal inheritance and zygotic deposition of H3K27me3 in mouse embryos.
Nature genetics    |    2021 Apr 20    |    PubMed ID: 33821003    |    Read Article

CAR directs T cell adaptation to bile acids in the small intestine
Nature    |    2021 Apr 07    |    PubMed ID: 33828301    |    Read Article

Osteoclasts recycle via osteomorphs during RANKL-stimulated bone resorption
Cell    |    2021 Mar 04    |    PubMed ID: 33636130    |    Read Article

Dynamic Regulation of Tfh Selection During the Germinal Center Reaction
Nature    |    2021 Feb 03    |    PubMed ID: 33536617    |    Read Article

Arterialization requires the timely suppression of cell growth
Nature    |    2021 Jan 26    |    PubMed ID: 33299176    |    Read Article

Microbiota-Induced Type I Interferons Instruct a Poised Basal State of Dendritic Cells.
Cell    |    2020 Dec 16    |    PubMed ID: 32380006    |    Read Article

SWI/SNF Complex Mutations Promote Thyroid Tumor Progression and Insensitivity to Redifferentiation Therapies
Cancer Discovery    |    2020 Dec 14    |    Read Article

>> ³í¹® ´õ º¸±â (¿¬°áµÇ´Â ÆäÀÌÁö ÇÏ´ÜÀ» Âü°íÇØÁÖ¼¼¿ä)

Keyword : Single cell,´ÜÀϼ¼Æ÷,½Ì±Û¼¿,single cell analysis,single cell NGS,full length,Ultra low input,low input,±Ø¼Ò·®,mRNA,mRNA-seq,cDNA,cDNA-seq,NGS,Next Generation Sequencing,SMART,smart-seq,SSv4

mRNA-Seq (Single cell)
>
Single cell Àü¿ëÀ¸·Î, ÃÖ°íÀÇ °¨µµ ¹× ¼º´ÉÀ¸..
[New]Single cell mRNA-Seq Kit
>
¼ö¸¹Àº CitationÀÌ Áõ¸íÇÏ´Â ±Ø¼Ò·® »ùÇà ºÐ¼® ..
[New]Ultra low input mRNA-Seq Kit
>
SMART-Seq mRNA ±â¹Ý, High-throughput mRNA-se..
[New]High throughput, Automation mRNA-Seq Kit
>
Single cell Àü¿ëÀ¸·Î, ÃÖ°íÀÇ °¨µµ ¹× ¼º´ÉÀ¸..
Single cell mRNA-Seq Kit
>
¼ö¸¹Àº CitationÀÌ Áõ¸íÇÏ´Â ±Ø¼Ò·® »ùÇà ºÐ¼® ..
Ultra low input mRNA-Seq Kit NEW!
>
SSv4 ±â¹Ý, High-throughput mRNA-seq ºÐ¼®
High throughput, Automation mRNA-Seq Kit
>
3¡¯ Differential Expression (DE) ºÐ¼®À» High..
Ultra low input 3¡¯end RNA-Seq Kit