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NGS °ü·Ã
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mRNA-Seq (Single cell)
> Ultra low input mRNA-Seq Kit
¼ö¸¹Àº CitationÀÌ Áõ¸íÇÏ´Â ±Ø¼Ò·® »ùÇà ºÐ¼® ÃÖ°ÀÚ
Ultra low input mRNA-Seq Kit
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(ºÎ°¡¼¼º°µµ)
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Clontech
634888
SMART-Seq¢ç v4 Ultra¢ç Low Input RNA Kit for Sequencing
12ȸ
(Package)
°¡°Ý¹®ÀÇ
Àç°í ¼ÒÁø ÈÄ Á¾¸Å
Clontech
634889
SMART-Seq¢ç v4 Ultra¢ç Low Input RNA Kit for Sequencing
24ȸ
(Package)
°¡°Ý¹®ÀÇ
Àç°í ¼ÒÁø ÈÄ Á¾¸Å
Clontech
634890
SMART-Seq¢ç v4 Ultra¢ç Low Input RNA Kit for Sequencing
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(Package)
°¡°Ý¹®ÀÇ
Àç°í ¼ÒÁø ÈÄ Á¾¸Å
Product index
PGT-A
Embgenix¢â PGT-A Kit (..
SMARTer RNA-Seq °³¿ä
[±â¼ú°³¿ä] SMARTer Tec..
RNA-Seq ¼±Åð¡À̵å
NGS ¼±Åð¡À̵å (NGS ±â..
RNA-Seq Á¦Ç°¸íÀÌ ÀÌ·¸..
mRNA-Seq (Single cell)
[New]Single cell mRNA-..
[New]Ultra low input m..
[New]High throughput, ..
Single cell mRNA-Seq K..
Ultra low input mRNA-S..
High throughput, Autom..
Ultra low input 3¡¯end..
Stranded RNA-seq
[New](+UMI) Pico i..
[New]Pico input strand..
[New]Single cell stran..
[New]Mid Input Strande..
[New]High Input Strand..
Total RNA-Seq Kit (FFP..
ÀÓ»ó»ùÇÃ ÃÖÀû Pico Inp..
ÀÓ»ó»ùÇÃ ÃÖÀû Pico Inp..
Single cell Stranded R..
Mid Input Stranded RNA..
High Input Stranded RN..
Small RNA-Seq
Small RNA-Seq Kit
Immune-Seq
[¼±Åð¡À̵å] Immune-se..
[New]UMI Àû¿ë Human TC..
[New]UMI Àû¿ë Human BC..
[New]UMI Àû¿ë Mouse TC..
[New]UMI Àû¿ë Mouse BC..
UMI Àû¿ë Human TCR pro..
Human BCR IgG IgM H/K/..
Mouse TCR a/b Profilin..
Mouse BCR IgG H/K/L Pr..
±âŸ RNA-Seq
Full length cDNA ÇÕ¼º
Target RNA Capture for..
DNA-seq °³¿ä
[±â¼ú°³¿ä] ThruPLEX & ..
DNA-Seq ¼±Åð¡À̵å
ChIP-Seq / Meth-Seq
50pg ChIP DNA-seq Kit
DNA SMART ChIP-Seq Kit
Meth-Seq DNA Enrichmen..
Micrococcal Nuclease
Low Input DNA-Seq
ThruPLEX¢ç DNA-seq Kit
ThruPLEX¢ç Plasma-seq ..
PicoPLEX¢ç Single Cell..
High input DNA-seq
ThruPLEX¢ç DNA-Seq HV
ThruPLEX¢ç Tag-Seq HV
Single cell DNA-Seq
PicoPLEX¢ç Single Cell..
Whole Genome Amplification..
[±â¼ú°³¿ä] ThruPLEX & ..
PicoPLEX¢ç Single Cell..
PicoPLEX¢ç Single Cell..
Index Kit
ThruPLEX, PicoPLEX¿ë I..
Stranded-SMART kit¿ë U..
SMART-PLUS kit¿ë UDI (..
Unique Dual Index Kits
NGS Library Á¤·®
Library Quantification..
NGS Sample preparation
rRNA Á¦°Å½Ã¾à RiboGone..
Magnetic Separator-PCR..
DNA Fragmentation Kit
NGS ºÐ¼®¿ë PCR È¿¼Ò
SeqAmp¢â DNA Polymeras..
PrimeSTAR¢ç GXL DNA Po..
PreAmp Master Mix
NGS °ü·Ã Àåºñ
ICELL8 cx Technical No..
ICELL8¢ç cx Single-Cel..
ICELL8 cx 3 Differenti..
ICELL8¢ç cx Single-Cel..
ICELL8 cx Human TCR Pr..
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Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
[Legacy] NGS Products
(Legacy) Human TCR a/b..
(Legacy) PicoPLEX¢ç WG..
* SMART-Seq
®
v4 Ultra
®
Low Input RNA Kit for Sequencing Á¦Ç°°ú SMART-Seq
®
v4 PLUS Kit Á¦Ç°Àº °¢°¢
SMART-Seq
®
mRNA
,
SMART-Seq
®
mRNA LP
·Î ¸®´º¾ó µÇ¾ú½À´Ï´Ù.
* UMIs ºÎ°¡·Î Á¤È®µµ¿Í ½Å·Úµµ¸¦ ³ôÀÎ ½ÅÁ¦Ç°,
SMART-Seq
®
mRNA LP (with UMIs)
°¡ Ãâ½ÃµÇ¾ú½À´Ï´Ù.
* PLUS kit´Â »ùÇ÷κÎÅÍ lllumina
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ºÐ¼®¿ë library Á¦ÀÛÀ» À§ÇÑ, library preparation kit¿Í single-use unique dual index (UDI)¸¦ Æ÷ÇÔÇÏ°í ÀÖ½À´Ï´Ù.
* ÀÚµ¿È ½Ã½ºÅÛ (Apollo¢â Library Prep System (½Ã½ºÅÛÀº ÆǸÅÁ¾·á))¿¡ Àû¿ë°¡´ÉÇÑ ½Ã¾à
-
SMART-Seq
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v4 Reagent Kit for the SMARTer¢â Apollo¢â System (Code 640170)
[°ü·Ã Á¤º¸ÀÚ·á]
-
SMART-Seq v4 Single cell RNA-seq ¿Â¶óÀÎ ¼¼¹Ì³ª ¹Ù·Îº¸±â
-
SMART-Seq v4 Ultra Low Input Kit¸¦ ÀÌ¿ëÇÑ Single cell RNA-Seq ³í¹® ¸®½ºÆ® º¸±â
10 pg ¼öÁØÀÇ ±Ø¼Ò·® total RNA¿¡µµ Àû¿ë °¡´É
Unparalleled sensitivity and reproducibility
Single-tube workflows -
cDNA ÇÕ¼ººÎÅÍ library Á¦ÀÛ °úÁ¤À» °¢°¢ single tube¿¡¼ ÁøÇàÇÒ ¼ö ÀÖ¾î, »ùÇà ¼Õ½ÇÀ̳ª ¿À¿° µî ½ÇÇè °úÁ¤¿¡¼ ¹ß»ýÇÒ ¼ö ÀÖ´Â ¿À·ù ÃÖ¼ÒÈ
High-quality RNA-seq data
- total RNA·ÎºÎÅÍ °¡Àå ¸¹Àº ¼öÀÇ À¯ÀüÀÚ ºÐ¼®
Increase your sequencing power
- ¸¹Àº »ùÇÃÀ» ºÐ¼®ÇÒ ¼ö ÀÖ´Â Unique dual index (UDI) »ç¿ë
High library yield -
³ôÀº yieldÀÇ library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ¾î,
NovaSeq¢â system°ú °°Àº high-throughput sequencer¿¡ Àû¿ëÇϰųª, ¿©·¯ Â÷·Ê ºÐ¼® °¡´É
Á¦Ç°¼³¸í
SMART-Seq
¢ç
v4 Ultra
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Low Input RNA Kit for Sequencing (SSv4)¿Í SMART-Seq
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v4 PLUS kit (SSv4 PLUS)´Â ±Ø¼Ò·® (Ultra-Low input) »ùÇÃÀ» À§ÇÑ SMARTer Ultra Low kit ½Ã¸®ÁîÀÇ 4¼¼´ë Á¦Ç°À¸·Î, ±Ø¼Ò·® »ùÇÃÀ» °¡Àå ¹Î°¨µµ ÀÖ°Ô ºÐ¼®ÇÒ ¼ö ÀÖ´Ù. SSv4 PLUS Á¦Ç°À» ÀÌ¿ëÇϸé, 1,000°³ ÀÌÇÏÀÇ intactÇÑ ¼¼Æ÷ ȤÀº 10 pg - 10 ngÀÇ high quality RNA (RIN > 8)·ÎºÎÅÍ oligo(dT) primer¸¦ ÀÌ¿ëÇØ °íÇ°Áú full-length cDNA¸¦ Á¦Á¶ÇÑ ÈÄ, lllumina
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ºÐ¼®¿ë library Á¦ÀÛ±îÁö ÁøÇàÇÒ ¼ö ÀÖ´Ù.
º» Á¦Ç°Àº SMART (Switching Mechanism at 5' End of RNA Template) ±â¼ú¿¡ Locked Nucleic Acid (LNA) ±â¼úÀ» Á¢¸ñÇÔÀ¸·Î½á template switching È¿À²À» ´õ¿í Çâ»ó½ÃÄ×´Ù. µû¶ó¼, ÀÌÀü ¼¼´ëÀÇ Á¦Ç°º¸´Ù ´õ¿í ¸¹Àº ¼öÀÇ À¯ÀüÀÚ¸¦ °ËÃâÇÒ ¼ö ÀÖ¾î Àü¹ÝÀûÀÎ Àü»çü ¿¬±¸ (full transcriptome analysis)¿¡ ÀÖ¾î ¸Å¿ì À¯¿ëÇÏ´Ù. SSv4´Â ³ôÀº ÀçÇö¼º°ú gene-body coverage »Ó ¾Æ´Ï¶ó GC-rich Àü»çüµµ Á¤È®ÇÏ°Ô cDNA·Î ÇÕ¼ºÇÒ ¼ö ÀÖ´Ù.
SMART-Seq
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v4 PLUS kit (SSv4 PLUS)¿¡ Æ÷ÇԵǾî ÀÖ´Â library preparation kit´Â enzymatic fragmentation¿Í stem-loop adaptor¸¦ ÇÔ²² »ç¿ëÇÏ¿©, 2½Ã°£ ³» single tube¿¡¼ purification °úÁ¤ ¾øÀÌ cDNA·ÎºÎÅÍ library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Ù. µû¶ó¼ »ùÇÃÀÇ ¼Õ½ÇÀ̳ª ¿À¿° µî ½ÇÇè °úÁ¤¿¡¼ ¹ß»ýÇÒ ¼ö ÀÖ´Â ¿À·ù¸¦ ÃÖ¼ÒÈ ÇÒ ¼ö ÀÖ´Ù. ÀÌ °úÁ¤À» ÅëÇØ Á¦ÀÛµÈ library´Â SMART-Seq2 µî ±âÁ¸ÀÇ ¹æ¹ý ¹× ´ç»ç ±âÁ¸ Á¦Ç° (SMART-Seq
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v3)¿¡ ºñÇØ ÀçÇö¼ºÀÌ ³ô°í, ÈξÀ ¿ì¼öÇÒ »Ó ¾Æ´Ï¶ó, ³ôÀº yield·Î Á¦À۵Ǿî high-throughput sequencer¿¡ Àû¿ëÇϰųª, ¿©·¯ Â÷·Ê ºÐ¼®ÇÒ ¼ö ÀÖ´Ù.
±×¸² 1. SMART±â¼úÀ» ÀÌ¿ëÇÑ cDNA ÇÕ¼º °úÁ¤
The SMART-Seq
¢ç
v4 Oligonucleotide, 3¡¯ SMART-Seq
¢ç
CDS Primer II A, and PCR Primer II A all contain a stretch of identical sequence. The black star indicates a chemical block on the 5¡¯ end of the oligonucleotide.
±×¸² 2. SSv4¸¦ ÀÌ¿ëÇßÀ» ¶§ ³ôÀº ¹Î°¨µµ¿Í mapping È¿À²
Replicate libraries were generated from 10 pg Mouse Brain Total RNA using the SMART-Seq
¢ç
v4 kit, the SMARTer
¢ç
Ultra Low v3 kit, or the SMART-Seq2 method. 18 PCR cycles were used to amplify cDNA libraries with the SMART-Seq2 method and SMARTer
¢ç
Ultra Low v3 kit; however, only 17 PCR cycles were needed for the SMART-Seq
¢ç
v4 libraries.
±×¸² 3. ±âÁ¸ ¹æ¹ýÀÎ SMART-Seq2, SSv3¿Í ºñ±³ÇßÀ» ¶§, SSv4ÀÇ ³ôÀº gene coverage
Gene body coverage shown is the average of two replicate libraries prepared from 10 pg Mouse Brain Total RNA using the three different cDNA synthesis methods. The SMARTer
¢ç
Ultra Low v3 kit produced a slight 3¡Ç bias, and the SMART-Seq
¢ç
v4 kit produced a slight 5¡Ç bias; however, the overall coverage was fairly even.
±×¸² 4. ³·Àº inputÀÇ »ùÇà »ç¿ë¿¡µµ ³ôÀº ÀçÇö¼º
FPKMs from replicate libraries generated from 10 pg of Mouse Brain Total RNA using the SMART-Seq
¢ç
v4 kit, the SMARTer
¢ç
Ultra Low v3 kit method were compared. For transcripts with FPKM <100, the correlation between replicates was much higher for the SMART-Seq
¢ç
v4 kit (Pearson R = 0.739; Panel B) compared to the SMARTer Ultra Low v3 (Pearson R = 0.376; Panel A). For all transcripts (shown in the scatterplots on the right) the correlation between replicates was high for each of the three methods (Pearson R between 0.911-0.972), though the SMART-Seq
¢ç
v4 kit did have the highest correlation. Transcripts represented in only one replicate can be seen along the X- and Y-axes of the scatter plots showing all transcripts.
±×¸² 5.
SSv4-HT PLUS
¸¦ ÀÌ¿ëÇÑ library¿¡¼ Nextera
¢ç
XTº¸´Ù ´õ ³ôÀº yield¿Í À¯ÀüÀÚ °ËÃâ
(Panel A)
SSv4 chemistry was used to produce cDNA, in triplicate, from 10 pg of Mouse Brain Total RNA. Illumina-compatible libraries were then generated using the SMART-Seq
¢ç
Library Prep Kit (not sold separately) portion of the SSv4 PLUS kit or Nextera
¢ç
XT and sequenced on a NextSeq 500. The reads were normalized to 4M paired-end reads and analyzed as described in the techical note.
(Panel B)
The library yield obtained with the SSv4 PLUS kit is higher than the yield obtained when using Nextera
¢ç
XT.
(Panel C)
The number of genes identified was also higher for the SSv4 PLUS kit compared to Nextera
¢ç
XT.
Àû¿ë
±Ø¼Ò·® »ùÇ÷κÎÅÍ full-length transcriptome ºÐ¼® (transcript isoforms, gene fusions, point mutations µî)À» À§ÇØ, cDNA ÇÕ¼º °úÁ¤¿¡¼ »ç¿ëÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°À» ÀÌ¿ëÇØ ÇÕ¼ºÇÑ cDNA´Â SMART-Seq Library Prep kit (SSv4 PLUS kit ³» Æ÷ÇÔ, º°µµ ±¸¸Å ºÒ°¡) ¸¦ ÀÌ¿ëÇÏ¿© Illumina
¢ç
ºÐ¼®¿ë sequencing library·Î Á¦ÀÛÇÒ ¼ö ÀÖ´Ù. ȤÀº Ion Xpress Plus Fragment Library Kit¸¦ ÀÌ¿ëÇÏ¿©, Ion Torrent sequencing¸¦ ÁøÇàÇÒ ¼ö ÀÖ´Ù.
ÀÚ¼¼ÇÑ ±¸¼ºÀº CoA¸¦ Âü°íÇϼ¼¿ä.
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Keyword : Single cell,´ÜÀϼ¼Æ÷,½Ì±Û¼¿,single cell analysis,single cell NGS,full length,Ultra low input,low input,±Ø¼Ò·®,mRNA,mRNA-seq,cDNA,cDNA-seq,NGS,Next Generation Sequencing,SMART,smart-seq,SSv4
mRNA-Seq (Single cell)
Single cell Àü¿ëÀ¸·Î, ÃÖ°íÀÇ °¨µµ ¹× ¼º´ÉÀ¸..
[New]Single cell mRNA-Seq Kit
¼ö¸¹Àº CitationÀÌ Áõ¸íÇÏ´Â ±Ø¼Ò·® »ùÇà ºÐ¼® ..
[New]Ultra low input mRNA-Seq Kit
SMART-Seq mRNA ±â¹Ý, High-throughput mRNA-se..
[New]High throughput, Automation mRNA-Seq Kit
Single cell Àü¿ëÀ¸·Î, ÃÖ°íÀÇ °¨µµ ¹× ¼º´ÉÀ¸..
Single cell mRNA-Seq Kit
¼ö¸¹Àº CitationÀÌ Áõ¸íÇÏ´Â ±Ø¼Ò·® »ùÇà ºÐ¼® ..
Ultra low input mRNA-Seq Kit
NEW!
SSv4 ±â¹Ý, High-throughput mRNA-seq ºÐ¼®
High throughput, Automation mRNA-Seq Kit
3¡¯ Differential Expression (DE) ºÐ¼®À» High..
Ultra low input 3¡¯end RNA-Seq Kit