´Ý±â
TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Å°¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø 
  • ¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢support@takara.co.kr
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢tkbd@takara.co.kr
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > Stranded RNA-seq > [New]High Input Stranded RNA-Seq Kit
High Input Strand- Specific Total RNA-Seq

[New]High Input Stranded RNA-Seq Kit

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
635046
SMART-Seq¢ç Total RNA High Input (RiboGone¢â Mammalian)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
24ȸ
(Package)
1,936,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x346372
Clontech
635047
SMART-Seq¢ç Total RNA High Input (RiboGone¢â Mammalian)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
96ȸ
(Package)
6,788,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
635045
SMART-Seq¢ç Total RNA High Input (RiboGone¢â Mammalian)
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
384ȸ (635047x4)
24,437,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

¡Ø º¯°æ»çÇ×
º» Á¦Ç°Àº ±âÁ¸ SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian (Code 634873)ÀÇ ¸®´º¾ó Á¦Ç°ÀÔ´Ï´Ù. Á¦Ç°»ç¿ë À¯¿¬¼ºÀ» À§ÇØ Á¦Ç° ±¸¼º¿¡¼­ index°¡ Á¦¿ÜµÇ¾ú½À´Ï´Ù. ÇÊ¿ä½Ã º°µµ±¸¸Å ¹Ù¶ø´Ï´Ù.
>> ȣȯµÇ´Â Unique Dual Index kits (UDI kits)

ÀÚ¼¼ÇÑ ³»¿ëÀº ´ÙÀ½ ¾È³»ÆäÀÌÁö¸¦ Âü°íÇϼ¼¿ä! Á¦Ç° ¼±ÅÃÀÌ ½¬¿öÁý´Ï´Ù.
>> RNA-Seq Á¦Ç°¸íÀÌ ÀÌ·¸°Ô ¹Ù²ò´Ï´Ù

  • ³ôÀº Á¤È®µµ: 99% ÀÌ»óÀÇ Á¤È®µµ·Î °¢ Àü»çüÀÇ origin °¡´Ú ½Äº° °¡´É
  • ºü¸£°í °£¼ÒÈ­µÈ ÇÁ·ÎÅäÄÝ: ´Ü 5½Ã°£ ¸¸¿¡ library Á¦ÀÛ
  • ³ÐÀº input ¹üÀ§: human, rat, mouseÀÇ total RNA 100 ng-1 ¥ìg
  • ´Ù¾çÇÑ »ùÇà Àû¿ë¼º: RIN (RNA Integrity Number) °ªÀÌ 1-3 Á¤µµÀÇ total RNA¿¡¼­µµ ³ôÀº ÀçÇö¼ºÀÇ °íÇ°Áú µ¥ÀÌÅ͸¦ »ý»ê
  • High throughput Illumina sequencing: 384°³ÀÇ unique index¸¦ »ç¿ëÇÏ¿© ÃÖ´ë 384°³ÀÇ »ùÇ÷ΠIllumina-ready library Á¦ÀÛ °¡´É
Á¦Ç° ¼³¸í
º» Á¦Ç°Àº 100 ng-1 §¶ÀÇ mammalian total RNA·ÎºÎÅÍ Illumina sequencing library¸¦ ÁغñÇϱâ À§ÇÑ È¿À²ÀûÀÎ ¼Ö·ç¼ÇÀ» Á¦°øÇÑ´Ù. ¶ÇÇÑ RiboGone¢â°ú SMART¢ç ±â¼úÀ» °áÇÕÇÏ¿© È¿À²ÀûÀ¸·Î rRNA¸¦ Á¦°ÅÇÏ°í strand ¹æÇ⼺ Á¤º¸°¡ À¯ÁöµÇ´Â libraryÀÇ ¿øÈ°ÇÑ »ý»êÀÌ °¡´ÉÇÏ´Ù.
rRNA´Â total RNAÀÇ ~90% ÀÌ»óÀ» Â÷ÁöÇϱ⠶§¹®¿¡ library »ý¼º Àü¿¡ rRNA°¡ È¿°úÀûÀ¸·Î Á¦°Å°¡ µÇ¾î¾ß sequencing ºñ¿ëÀ» ³·Ãß°í mapping statistics¸¦ Çâ»ó½Ãų ¼ö ÀÖ´Ù.
SMART¢ç ±â¼úÀ» ÀÌ¿ëÇÑ cDNA ÇÕ¼º °úÁ¤°ú PCRÀ» ÅëÇØ Unique Dual Index Kits (Code 634752-634756, º°µµ±¸¸Å)¿¡ ÀÖ´Â index ¹× adaptor¸¦ Ãß°¡ÇÏ°í, strand ¹æÇ⼺À» ºÎ¿©ÇÏ¿© °£´ÜÇÏ°Ô library prep ÇÁ·Î¼¼½º¸¦ ¿Ï·áÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ rRNA Á¦°ÅºÎÅÍ Illumina¿ë index°¡ Ãß°¡µÈ RNA-Seq library Áغñ±îÁö ¾à 5½Ã°£ ³» ¿Ï¼ºµÈ´Ù.

*±âŸ input amount¿¡ µû¸¥ Ãßõ Á¦Ç° :
- Total RNA 250 pg-10 ng : SMARTer¢ç Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian (Code 634485 ¿Ü)
- Total RNA 10-100 ng : SMART-Seq¢ç Total RNA Mid Input (Code 635049 ¿Ü)
Àû¿ë
  • Illumina Ç÷§ÆûÀ» ÀÌ¿ëÇÑ mammalian sampleÀÇ RNA-Seq
  • Strand ¹æÇ⼺ Á¤º¸¸¦ À¯ÁöÇÏ´Â NGS library Áغñ
  • coding ¹× noncoding ¼­¿­ Á¤º¸ ºÐ¼®
ÀÚ¼¼ÇÑ ±¸¼ºÀº CoA¸¦ Âü°íÇϼ¼¿ä.
RNA-Seq ·¯´×¼¾Å͸¦ ÅëÇØ º» Á¦Ç°¿¡ ´ëÇÑ ´õ ¸¹Àº µ¥ÀÌÅÍ¿Í FAQ¸¦ Âü°íÇϼ¼¿ä.

Keyword : BioMarker,FFPE,Cell-free RNA,Degraded RNA,Non-coding RNA,ncRNA,lncRNA,Cancer,Transcriptomics,transcriptome,LCM,stranded-seq,smarter,random primer,smart-seq

Stranded RNA-seq
>
(with UMI) FFPE, LCM, cell-free RNAµîÀÇ ±Ø¼Ò..
[New](+UMI) Pico input stranded RNA-Seq Kit
>
FFPE, LCM, cell-free RNAµîÀÇ ±Ø¼Ò·® low qual..
[New]Pico input stranded RNA-Seq Kit
>
´ÜÀϼ¼Æ÷ (Single cell)¿¡¼­ stranded RNA-Seq ..
[New]Single cell stranded RNA-Seq Kit
>
Mid Input Strand- Specific Total RNA-Seq
[New]Mid Input Stranded RNA-Seq Kit
>
High Input Strand- Specific Total RNA-Seq
[New]High Input Stranded RNA-Seq Kit
>
UDI, UMI Àû¿ëÀ¸·Î ´õ¿í Ưº°ÇØÁø
ÀÓ»ó»ùÇÃ ÃÖÀû Pico Input Mammalian(v3)
>
Picogram RNA·ÎºÎÅÍ Strand-specific illumina ..
ÀÓ»ó»ùÇÃ ÃÖÀû Pico Input Mammalian(v2)
>
´ÜÀϼ¼Æ÷ (single cell)ÀÇ stranded RNA-Seq li..
Single cell Stranded RNA-Seq Kit
>
High Input Strand- Specific Total RNA-Seq
High Input Stranded RNA-Seq Kit