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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > Spatial analysis > Takara Trekker¢ç FX
FFPE Á¶Á÷ÀÇ true single cell °ø°£ ºÐ¼® ½ÇÇö

Takara Trekker¢ç FX

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Clontech
SK023
Trekker¢ç FX 10x10 Bundle
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Clontech
SK024
Trekker¢ç FX 10x10 Training Bundle
°ü·ÃÇмú ±¸¸ÅÇϱâ new ¶óÀ̼±½º 
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°¡°Ý¹®ÀÇ   Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x356533
Clontech
SK025
Trekker¢ç Alignment Fixture Bundle
°ü·ÃÇмú ±¸¸ÅÇϱâ new
1 kit
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Clontech
K011
Trekker Starter Kit Bundle (UV lamp)
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 kit
°¡°Ý¹®ÀÇ   Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

  • Trekker¢ç Single-Cell Spatial Mapping KitÀÇ Slide tags[1] ±â¼ú ±â¹Ý
    [1] Nature Vol 625, pp. 101-109 (2024).
  • FFPE »ùÇÃÀÇ true single cell °ø°£ºÐ¼® °¡´É
  • Single cell ºÐ¼® µ¥ÀÌÅÍ¿¡ À§Ä¡ Á¤º¸¸¦ ºÎ¿©ÇÏ¿© ºÐ¼®
  • ¼¼Æ÷ ³» ÇÙÀ» DNA barcode·Î taggingÇÏ´Â ¹æ½ÄÀ¸·Î º°µµÀÇ single cell segmentation ¾øÀÌ Á¤È®ÇÑ °ø°£ºÐ¼® ½ÇÇö
  • º» Á¦Ç°À¸·Î taggingÇÏ¿© ÃßÃâÇÑ ÇÙÀ» ÈÄ¼Ó single cell NGS ºÐ¼® ¿öÅ©Ç÷οì (e.g. 10X Chromium)¿¡ Àû¿ë
  • º°µµÀÇ °í°¡ ÀåÄ¡³ª probe set ºÒÇÊ¿ä (Á¾¿¡ »ó°ü¾øÀÌ ºÐ¼® °¡´É)
  • ÀϹÝÀûÀÎ À¯¸® ½½¶óÀ̵å·Î FFPE Á¶Á÷ ÀýÆí Áغñ: tagging ½Ã À§Ä¡Á¶Á¤ÀÌ ½¬¿ò
  • ÇÊ¿ä ½Ã H&E staining ÈÄ Trekker¢ç FX Kit·Î °ø°£ ºÐ¼® °¡´É
Á¦Ç°¼³¸í
Trekker¢ç FX´Â FFPE »ùÇÃÀ» ´ë»óÀ¸·Î single cell °ø°£ Àü»çü (spatial transcriptomics) ÇØ¼®À» Á¦°øÇÑ´Ù. NGS library Á¦ÀÛ Àü ´Ü°è¿¡ Àû¿ëÇÏ¿© single nuclei¿¡ À§Ä¡ Á¤º¸ (Spatial barcode)¸¦ ºÎ¿©ÇÔÀ¸·Î½á true single cell °ø°£ ºÐ¼®ÀÌ °¡´ÉÇÏ´Ù.

º» Á¦Ç°¿¡´Â À§Ä¡ Á¤º¸ (Spatial barcode)¸¦ °¡Áø DNA barcode°¡ °áÇÕµÈ bead (Á÷°æ 10 ¥ìm)°¡ ´ÜÃþÀ¸·Î ¹è¿­µÈ Tile Slide°¡ Æ÷ÇԵǾî ÀÖ´Ù. ÀÌ Tile Slide¿Í ºÐ¼®ÇϰíÀÚ ÇÏ´Â FFPE »ùÇà Á¶Á÷ÀÌ ºÎÂøµÈ ½½¶óÀ̵带 Trekker¢ç Alignment Fixture (Code SK025)·Î ÀÌ¿ëÇØ ¹ÐÂø½ÃŲ´Ù. UV Á¶»ç¿¡ ÀÇÇØ DNA barcode°¡ bead·ÎºÎÅÍ ºÐ¸®µÇ¾î ÁÖº¯ÀÇ ÇÙ ³»ºÎ·Î ħÅõÇϱ⠶§¹®¿¡ °¢°¢ÀÇ ÇÙ¿¡ Á÷Á¢ À§Ä¡ Á¤º¸¸¦ ºÎ¿©ÇÏ´Â °ÍÀÌ °¡´ÉÇÏ´Ù. ±× ÈÄ ÇÙÀ» ÃßÃâÇÏ¿© single-nucleus RNA sequencing (snRNA-Seq)À» ÁøÇàÇÏ¸é °¢°¢ÀÇ ÇÙ¿¡¼­ À¯·¡ÇÑ À¯ÀüÀÚ Á¤º¸ ¹× À§Ä¡ Á¤º¸¸¦ Æ÷ÇÔÇÑ Àü¹ÝÀûÀÎ ÇØ¼®ÀÌ °¡´ÉÇÏ´Ù. ¶ÇÇÑ ¿É¼ÇÀ¸·Î, µ¿ÀÏÇÑ FFPE Á¶Á÷ ÀýÆí¿¡¼­ H&E staining ºÐ¼®À» Ãß°¡·Î ÁøÇàÇÒ ¼ö ÀÖ´Ù.

Trekker¢ç FX 10x10 Training Bundle (Code SK024)Àº FFPE Á¶Á÷ ÀýÆíÀ» Tile Slide¿¡ ºÎÂøÇÏ´Â °ÍºÎÅÍ ÇÙ ÃßÃâ±îÁöÀÇ ½ÇÇè °úÁ¤À» °ËÁõ ¹× ÃÖÀûÈ­Çϱâ À§ÇÑ Æ®·¹ÀÌ´× kit Á¦Ç°ÀÌ´Ù. Trekker¢ç FX 10x10 Bundle (Code SK023)·Î º» ½ÇÇèÀ» ÁøÇàÇϱâ Àü¿¡, ÇØ´ç Æ®·¹ÀÌ´× kit¸¦ ÅëÇØ »ç¿ëÀÚÀÇ »ùÇÃÀÌ Á¦Ç° ¿öÅ©Ç÷ο쿡 ¾Ë¸Â°Ô ÀÛµ¿ÇÏ´Â Áö È®ÀÎÇÏ´Â µî ÃÖÀûÈ­ ´Ü°è¸¦ ÁøÇàÇÒ °ÍÀ» ±ÇÀåÇÑ´Ù.
¡Øº» Æ®·¹ÀÌ´× kit (Trekker¢ç FX 10x10 Training Bundle, Code SK024)´Â snRNA-Seq ºÐ¼® ÁøÇà ºÒ°¡

±×¸² 1. Trekker¢ç Tile Slide
  • Photocleavable linker: UV Á¶»ç¿¡ ÀÇÇØ bead·ÎºÎÅÍ ºÐ¸®µÇ´Â ºÎºÐ
  • BB-1, Linker, BB-2: À§Ä¡Á¤º¸¸¦ ³ªÅ¸³»´Â ¼­¿­·Î, °¢ bead¸¶´Ù °íÀ¯ÇÑ ¼­¿­À» °¡Áü
  • Handshake sequence: Single cell system¿¡ ÀÇÇØ Æ÷ȹµÇ´Â ¼­¿­·Î, À̸¦ ÅëÇØ bead barcode°¡ mRNA µî ºÐ¼® ´ë»ó°ú ÇÔ²² µ¿½Ã¿¡ ½ÃÄö½ÌµÊ


±×¸² 2. Trekker¢ç FX ¿öÅ©Ç÷οì
Trekker¢ç Tile Slide¿Í FFPE Á¶Á÷ÀÌ ºÎÂøµÈ ½½¶óÀ̵带 Trekker¢ç Alignment Fixture¸¦ ÀÌ¿ëÇØ sandwich ÇüÅ·Π¹ÐÂø½ÃÄÑ, Á¶Á÷°ú bead °£ÀÇ taggingÀ» ¼öÇàÇÑ´Ù. ÇÙÀ» À§Ä¡ Á¤º¸°¡ ºÎ¿©µÈ DNA barcode·Î taggingÇÔÀ¸·Î½á, single cell ¼öÁØÀÇ Á¤È®ÇÑ °ø°£ ÇØ¼®ÀÌ °¡´ÉÇϸç, Á¶Á÷ ³» ¼¼Æ÷ÀÇ À§Ä¡ Á¤º¸¸¦ snRNA-Seq µ¥ÀÌÅÍ¿¡ mappingÇÒ ¼ö ÀÖ´Ù.
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[Trekker¢ç FX ¸ÞÀÎ Á¦Ç° ±¸¼ºÇ° Á¤º¸]

Á¦Ç°ÄÚµå

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SK017

Trekker¢ç FX 10x10 Bundle

4 Rxns

±¸¼ºÇ°

Trekker¢ç FX 10x10 Tiles

4 Tiles

Trekker¢ç FX Reagent Kit

4 Rxns

Trekker¢ç FX Accessories

4 Rxns

SK024*

Trekker¢ç Alignment Fixture Bundle*

1 kit

±¸¼ºÇ°

Trekker¢ç Alignment Fixture

1 ea

Alignment Fixture Spacers

6 ea

K011*

Trekker¢ç Starter Kit Bundle (UV lamp)*

1 kit

*Trekker¢ç FX ¿öÅ©Ç÷οì ÃÖÃÊ ¼¼ÆÃ ½Ã 1ȸ¸¸ ±¸¸Å


±×¸² 3. Trekker¢ç Starter Kit Bundle (UV lamp)¿Í Trekker¢ç Alignment Fixture Bundle Á¦Ç° »çÁø
Penal A. Trekker¢ç Starter Kit BundleÀÇ UV lamp. Penal B. Trekker¢ç Alignment Fixture BundleÀÇ Trekker¢ç Alignment Fixture Penal C. Trekker¢ç Alignment Fixture À§¿¡ UV lamp¸¦ ÀåÂøÇÑ ¸ð½À

[Trekker¢ç FX Training Kit ±¸¼ºÇ° Á¤º¸]
Trekker¢ç FX Training Kit´Â FFPE Á¶Á÷ ÀýÆíÀ» tile¿¡ Àû¿ëÇϰí nuclei dissociation ´Ü°è¸¦ ¿¬½ÀÇÒ ¼ö ÀÖ´Â kitÀÌ´Ù. (snRNA-seq ÁøÇà ºÒ°¡)

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SK017

Trekker¢ç FX 10x10 Bundle

4 Rxns

±¸¼ºÇ°

Trekker¢ç FX 10x10 Training Tiles

2 Tiles

Trekker¢ç FX Reagent Kit

4 Rxns

Trekker¢ç FX Accessories

4 Rxns

¡ØTraining kit »ç¿ë ½Ã, Trekker¢ç Alignment Fixture Bundle (Code SK024)°ú Trekker¢ç Starter Kit Bundle (UV lamp) (Code K011) ±¸¸Å ÇÊ¿ä. (ÃÖÃÊ ¼¼ÆÃ ½Ã 1ȸ¸¸ ±¸¸Å)
ÈÄ¼Ó application protocol
- 10x Single Cell Flex Multiplex with Feature Barcoding: Trekker¢ç FX Single-Cell Spatial Mapping Kit User Manual
°ü·Ã Á¦Ç°
- ´Ù¾çÇÑ application¿¡ Àû¿ë °¡´ÉÇÑ fresh frozen tissue Àü¿ë Trekker kit: Takara Trekker¢ç kit
- Àü¿ë ÀåÄ¡ ¾øÀÌ whole transcriptome °ø°£ ºÐ¼®À» À§ÇÑ reagent kit: Takara Seeker¢â kit

Keyword :

Spatial analysis
>
Àü¿ë ÀåÄ¡ ÇÊ¿ä ¾øÀÌ whole transcriptome °ø°£..
Takara Seeker Spatial Transcriptomics Kit
>
True single cell °ø°£ ºÐ¼®ÀÌ °¡´ÉÇÑ reagent ..
Takara Trekker U Single-cell Spatial Mapping Kit
>
FFPE Á¶Á÷ÀÇ true single cell °ø°£ ºÐ¼® ½ÇÇö
Takara Trekker¢ç FX