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NGS °ü·Ã
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NGS Sample preparation
> rRNA Á¦°Å½Ã¾à RiboGone¢â-Mammalian
Human, mouse, rat total RNA·ÎºÎÅÍ rRNA ¹× mtRNA¸¦ È¿°úÀûÀ¸·Î Á¦°Å
rRNA Á¦°Å½Ã¾à RiboGone¢â-Mammalian
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(ºÎ°¡¼¼º°µµ)
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»ç¿ëÀڸŴº¾ó
Clontech
634846
RiboGone-Mammalian
6ȸ
600,800¿ø
751,000¿ø
11.01 ~ 12.27
Clontech
634847
RiboGone-Mammalian
24ȸ
1,743,200¿ø
2,179,000¿ø
11.01 ~ 12.27
Product index
PGT-A
Embgenix¢â PGT-A Kit (..
SMARTer RNA-Seq °³¿ä
[±â¼ú°³¿ä] SMARTer Tec..
RNA-Seq ¼±Åð¡À̵å
NGS ¼±Åð¡À̵å (NGS ±â..
RNA-Seq Á¦Ç°¸íÀÌ ÀÌ·¸..
mRNA-Seq (Single cell)
[New]Single cell mRNA-..
[New]Ultra low input m..
[New]High throughput, ..
Ultra low input mRNA-S..
High throughput, Autom..
Ultra low input 3¡¯end..
Stranded RNA-seq
[New](+UMI) Pico i..
[New]Pico input strand..
[New]Single cell stran..
[New]Mid Input Strande..
[New]High Input Strand..
Total RNA-Seq Kit (FFP..
ÀÓ»ó»ùÇÃ ÃÖÀû Pico Inp..
ÀÓ»ó»ùÇÃ ÃÖÀû Pico Inp..
Single cell Stranded R..
Mid Input Stranded RNA..
High Input Stranded RN..
Small RNA-Seq
Small RNA-Seq Kit
Immune-Seq
[¼±Åð¡À̵å] Immune-se..
[New]UMI Àû¿ë Human TC..
[New]UMI Àû¿ë Human BC..
[New]UMI Àû¿ë Mouse TC..
[New]UMI Àû¿ë Mouse BC..
UMI Àû¿ë Human TCR pro..
Human BCR IgG IgM H/K/..
Mouse TCR a/b Profilin..
Mouse BCR IgG H/K/L Pr..
±âŸ RNA-Seq
Full length cDNA ÇÕ¼º
Target RNA Capture for..
DNA-seq °³¿ä
[±â¼ú°³¿ä] ThruPLEX & ..
DNA-Seq ¼±Åð¡À̵å
ChIP-Seq / Meth-Seq
50pg ChIP DNA-seq Kit
DNA SMART ChIP-Seq Kit
Meth-Seq DNA Enrichmen..
Micrococcal Nuclease
Low Input DNA-Seq
ThruPLEX¢ç DNA-seq Kit
ThruPLEX¢ç Plasma-seq ..
PicoPLEX¢ç Single Cell..
High input DNA-seq
ThruPLEX¢ç DNA-Seq HV
ThruPLEX¢ç Tag-Seq HV
Single cell DNA-Seq
PicoPLEX¢ç Single Cell..
Whole Genome Amplification..
[±â¼ú°³¿ä] ThruPLEX & ..
PicoPLEX¢ç Single Cell..
PicoPLEX¢ç Single Cell..
Index Kit
ThruPLEX, PicoPLEX¿ë I..
Stranded-SMART kit¿ë U..
SMART-PLUS kit¿ë UDI (..
Unique Dual Index Kits
NGS Library Á¤·®
Library Quantification..
NGS Sample preparation
rRNA Á¦°Å½Ã¾à RiboGone..
Magnetic Separator-PCR..
DNA Fragmentation Kit
NGS ºÐ¼®¿ë PCR È¿¼Ò
SeqAmp¢â DNA Polymeras..
PrimeSTAR¢ç GXL DNA Po..
PreAmp Master Mix
NGS °ü·Ã Àåºñ
ICELL8 cx Technical No..
ICELL8¢ç cx Single-Cel..
ICELL8 cx 3 Differenti..
ICELL8¢ç cx Single-Cel..
ICELL8 cx Human TCR Pr..
ICELL8¢ç cx blank chip
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
[Legacy] NGS Products
(Legacy) PicoPLEX¢ç WG..
RiboGone-Mammalian kit´Â human, mouse, ratÀÇ total RNA »ùÇ÷κÎÅÍ
5S, 5.8S, 18S, 28S
nuclear rRNA (Ribosomal RNA) »Ó¸¸ ¾Æ´Ï¶ó
12S
mtRNA (mitochondrial RNA)¸¦ Á¦°ÅÇÏ´Â ½Ã¾àÀÌ´Ù. ƯÈ÷, RiboGoneÀº FFPE »ùÇðú °°ÀÌ degradationµÈ »ùÇûӸ¸ ¾Æ´Ï¶ó ³·Àº qualityÀÇ RNA »ùÇÿ¡µµ Àû¿ë °¡´ÉÇÏ´Ù. RiboGone-Mammalian Kit´Â random-primed cDNA synthesis¿Í RNA-seq ¶Ç´Â transcriptom ºÐ¼® ½ÇÇè Àü, sample preparation °úÁ¤¿¡ ÀûÇÕÇÏ´Ù (including the SMARTer Stranded RNA-Seq Kit
(634836)
, the SMARTer Universal Low Input RNA Kit for Sequencing
(634938)
, and the SMARTer Universal Low Input RNA Library Prep Kit
(634947)
) (±×¸² 1).
±×¸² 1. RiboGone - Mammalian is specifically designed to work with low input RNA samples containing between 10-100 ng of full-length or degraded total RNA.
Samples processed using the RiboGone kit are ready for cDNA synthesis with any random-primed SMARTer RNA-Seq kit (including the SMARTer Stranded RNA-Seq Kit, the SMARTer Universal Low Input RNA Kit for Sequencing, and the SMARTer Universal Low Input RNA Library Prep Kit).
Ư¡
Effective rRNA removal
- rRNA removal method for human, mouse and rat total RNA samples - depletes ¡Ã95% of ribosomal RNA sequences
Suitable for any quality RNA
- designed to work with any quality starting RNA, including degraded material like FFPE samples
Low input RNA working range
- 10 ng -100 ng starting material
Works seamlessly
with downstream random-primed cDNA synthesis kit like the Stranded RNA-Seq Kit or SMARTer Universal Low Input RNA Library Prep Kit
±×¸² 2. Comparison of Human Universal Reference RNA and Human Brain Reference RNA sequencing alignment
metrics for 10 ng of RNA treated with the RiboGone - Mammalian kit and the SMARTer Stranded RNA-Seq Kit show that RiboGone efficiently degrades rRNA from small total RNA samples.
±×¸² 3. Sequencing data of RiboGone
- Mammalian treated RNA samples highly correlate with MAQC qPCR data for the same reference RNA. The high level of correlation between the RNA-Seq and MAQC data (R=0.860) shows that depletion of rRNA with RiboGone does not interfere with detection of other genes by RNA-seq.
Àû¿ë
Removal of rRNA from small total RNA samples prior to random-primed cDNA synthesis
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ±¸¼ºÇ°Àº CoA ¶Ç´Â ¸Å´º¾ó ÂüÁ¶)
5X RiboGone Hyb Buffer
RNaseH (5 U/ul)
DNaseI (5 U/ul)
RNase Inhibitor (40 U/ul)
Nuclease-Free Water RiboGone Purification Buffer
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Keyword : rrna Á¦°Å,ribosomal RNA,rRNA,rrna depletion,total rna,ribozero,ribo-zero,¸®º¸°ï,depletion
NGS Sample preparation
Human, mouse, rat total RNA·ÎºÎÅÍ rRNA ¹× mt..
rRNA Á¦°Å½Ã¾à RiboGone¢â-Mammalian
Magnetic bead-based DNA purification & size ..
Magnetic Separator-PCR Strip
DNA Fragmentation Kit