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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > NGS °ü·Ã Àåºñ > ICELL8 cx 3 Differential Expression(DE) Kit

ICELL8 cx 3 Differential Expression(DE) Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
640167
ICELL8¢ç 3' DE Reagent Kit
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1 Rxn
(Package)
°¡°Ý¹®ÀÇ  
Takara
640005
ICELL8¢ç 3' DE for UMI Reagent Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 Kit
°¡°Ý¹®ÀÇ  
Takara
640197
ICELL8¢ç cx Loading Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 Rxn
°¡°Ý¹®ÀÇ   ICELL8 cx Human TCR ab Profiling User Manual_030920.pdf
Takara
640199
ICELL8¢ç cx 3' DE Chip
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 Chip
°¡°Ý¹®ÀÇ  
Takara
640048
ICELL8¢ç Collection Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 Kit
°¡°Ý¹®ÀÇ  
Takara
640018
MSND 384-Well Source Plate and Seals (430-000025)
°ü·ÃÇмú ±¸¸ÅÇϱâ
20/Pack
°¡°Ý¹®ÀÇ  

  • High-throughput Single Cell analysis : 1,200~1,500°³ÀÇ ´ÜÀϼ¼Æ÷ÀÇ sequence µ¥ÀÌÅ͸¦ Çѹø¿¡ ȹµæ
  • ÇÑ ¹ø ½ÇÇè¿¡¼­ ÃÖ´ë 8°¡Áö ´Ù¸¥ ¼¼Æ÷ »ùÇà ºÐ¼® °¡´É
  • mRNA 3' ¸»´Ü¿¡¼­ À¯·¡ÇÑ ¼­¿­¸¸ Çؼ®ÇÏ¿© À¯ÀüÀÚ ¹ßÇö Â÷ÀÌ ºÐ¼®¿¡ ÇÊ¿äÇÑ Read ¼ö¸¦ ÁÙÀÏ ¼ö ÀÖÀ½.
  • Illumina»ç NGS Sequencer platformÀûÇÕÇÑ library Á¦ÀÛ

SMART technology¸¦ ÀÌ¿ëÇÑ ´ÜÀϼ¼Æ÷ RNA-seq library±¸Ãà°ú SMARTer ICELL8½Ã½ºÅÛÀÇ ¼¼Æ÷ºÐ¸®¿Í ¼¼Æ÷¼±ÅôɷÂÀ» Á¶ÇÕÇÏ¿©, ÃÖ´ë 8Á¾·ùÀÇ »ùÇ÷κÎÅÍÀÇ ´ÜÀÏ ¼¼Æ÷ÀÇ gene differential expression Çؼ®À» À§ÇÑ Illumina sequencer library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ½À´Ï´Ù.
¼¼Æ÷ »ùÇÃÀÇ ¿°»ö ¹× Èñ¼® ÈÄ SMARTer ICELL8 cx½Ã½ºÅÛÀ» »ç¿ëÇÏ¿© ¼¼Æ÷¸¦ SMARTer ICELL8 cx 3'DE Chip»óÀÇ 5,184°³ÀÇ nano well¿¡ ºÐÁÖÇÕ´Ï´Ù. ±× ÈÄ, Àü¿ë ¼ÒÇÁÆ®¿þ¾î CellSelect Software¸¦ »ç¿ëÇÏ¿©, »ì¾ÆÀÖ´Â ´ÜÀÏ ¼¼Æ÷¸¦ Æ÷ÇÔÇÑ wellÀ» ¼±ÅÃÇÕ´Ï´Ù. Çؼ®ÇÏ°íÀÚÇÏ´Â ¼¼Æ÷¸¦ ¼±ÅÃÇÑ ÈÄ ±× ÇØ´ç well¿¡ RT-PCR½Ã¾àÀ» ºÐÁÖ, 1ȸ ¹ÝÀÀÀ¸·Î cDNAÇÕ¼º ¹× ÁõÆøÀ» chip ³»¿¡¼­ ½Ç½ÃÇÕ´Ï´Ù. RT-PCRÈÄ °¢ wellÀÇ cDNA¸¦ ÇÑ tube¿¡ ¸ð¾Æ¼­, Á¤Á¦ÇÏ°í transposase¿¡ ±Ù°ÅÇÑ ¹æ¹ýÀ¸·Î mRNA 3'¸»´Ü cDNA¸¦ ³óÃàÇÑ µÚ Illumina index ¸¦ ³Ö°í, PCRÁõÆøÇÏ¿© sequencing¿ë library¸¦ ÁغñÇÕ´Ï´Ù.

3'DE Çؼ®¿¡ UMIÀ» »ç¿ëÇÏ´Â °æ¿ì¿Í UMIÀ» »ç¿ëÇÏÁö ¾Ê´Â °æ¿ì ÇÊ¿äÇÑ ½Ã¾àÀÌ ´Ù¸¨´Ï´Ù. ÀÚ¼¼ÇÑ °ÍÀº ¾Æ·¡ÀÇ Ç¥ 1.À» Âü°íÇØ ÁÖ¼¼¿ä.

Ç¥ 1. 3'DE¹ßÇö Çؼ®¿¡ UMIÀ» »ç¿ëÇÏ´Â °æ¿ì¿Í UMIÀ» »ç¿ëÇÏÁö ¾ÊÀ» °æ¿ìÀÇ Á¦Ç° ¼±Åÿ¡ ´ëÇؼ­

UMI¸¦ »ç¿ëÇÏÁö ¾ÊÀ» °æ¿ì

UMI¸¦ »ç¿ëÇÏ´Â °æ¿ì

ÇÊ¿ä½Ã¾à

¤ý SMARTer ICELL8 cx 3' DE Chip(Code 640199)

¤ý SMARTer ICELL8 Collection Kit(Code 640048)

¤ý SMARTer ICELL8 cx Loading Kit(Code 640197)

¤ý SMARTer ICELL8 3' DE Reagent Kit(Code 640167)
: ¿ªÀü»çÈ¿¼Ò Æ÷ÇÔ

¤ý SMARTer ICELL8 3' DE for UMI Reagent Kit (Code 640005)
: ¿ªÀü»ç È¿¼Ò ¹ÌÆ÷ÇÔ, º°µµ ±¸¸Å

protocol

1 Step RT-PCR

2 Step RT-PCR

Cell lysis

µ¿°áÀ¶ÇØ (-80¡É, 30 min)¿Í Triton

µ¿°áÀ¶ÇØ (-80¡É, 30 min)


MSND 384-well Source Plate and Seals´Â ¼¼Æ÷ ÇöŹ¾×(Cell suspension)ÀÇ ºÐÁÖ¿¡ ÀÌ¿ëÇÏ´Â source plate¿Í sealÀÇ ¼¼Æ®ÀÔ´Ï´Ù. Ç¥ 1ÀÇ ÇÊ¿ä ½Ã¾à ¿Ü¿¡ ±¸ÀÔÀÌ ÇÊ¿äÇÕ´Ï´Ù.

Cell suspension Áغñ Á¶°Ç°ËÅä¿Í ºÐÁÖÅ×½ºÆ®¸¦ ÇÒ ¶§¿¡´Â °¢ well¿¡ preprinted oligo(dT) primer°¡ ¹ÌÆ÷ÇÔµÈ Blank ChipÀ» ÀÌ¿ëÇϽʽÿÀ.
³»¿ë
SMARTer ICELL8 3' DE Reagent Kit(Code 640167)
¤ýBox 1: Control K562 Total RNA (1 ¥ìg/¥ìl)
¤ýBox 2: MgCl2 (1 M)
¤ý5X First-Strand Buffer
¤ýSeqAmp PCR Buffer (2X)
¤ýSMARTScribe Reverse Transcriptase (100 U/¥ìl)
¤ýSeqAmp DNA Polymerase
¤ýTriton X-100 (10£¥)
¤ýSMARTer ICELL8 3' DE Oligo Mix
¤ýDTT (100 mM)
¤ýGC Melt (5 M)
¤ýdNTP Mix (25 mM each)
¤ýSecond Diluent (100X)
¤ýICELL8 Fiducial Mix (1X)
¤ýRNase Inhibitor (40 U/¥ìl)
¤ýPrimer P5 (5 ¥ìM)
¤ýNuclease-Free Water

SMARTer ICELL8 3¡¯ DE for UMI Reagent Kit (Code 640005)
¤ýFiducial Mix
¤ýSecond Diluent
¤ýAmplification Primer
¤ýRT E5OLIGO
¤ýNextera Primer P5

SMARTer ICELL8 Collection Kit (Code 640048)
¤ýCollection Fixture
¤ýCollection Tube
¤ýCollection Film

SMARTer ICELL8 cx Loading Kit (Code 640197)
¤ýOptical Imaging Film
¤ýSealing Film
¤ýBlotting Paper
¤ýSmartChip Intermediate Film

MSND 384-well Source Plate and Seals (Code 640018)
¤ý2 packs 384-Well Source Plate Seals (10 per pack)
¤ý1 pack 384-Well Source Plate (20 per pack)
º¸Á¸
SMARTer ICELL8 cx 3' DE Chip : ½Ç¿Â
SMARTer ICELL8 Collection Kit : ½Ç¿Â
SMARTer ICELL8 cx Loading Kit : ½Ç¿Â
MSND 384-well Source Plate and Seals : ½Ç¿Â
Control K562 RNA-70¡É
±âŸ : -20¡É



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