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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > Stranded RNA-seq > [New]Single cell stranded RNA-Seq Kit
´ÜÀϼ¼Æ÷ (Single cell)¿¡¼­ stranded RNA-Seq library Á¦ÀÛ °¡´É

[New]Single cell stranded RNA-Seq Kit

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Clontech
634360
SMART-Seq¢ç Total RNA Single Cell (ZapR¢â Mammalian)
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24 ȸ (Package)
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Clontech
634361
SMART-Seq¢ç Total RNA Single Cell (ZapR¢â Mammalian)
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96 ȸ (Package)
8,072,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
634362
SMART-Seq¢ç Total RNA Single Cell (ZapR¢â Mammalian)
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384 ȸ (634355x4)
29,059,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

¡Ø º¯°æ»çÇ×
º» Á¦Ç°Àº ±âÁ¸ SMART-Seq¢ç Stranded Kit (Code 634442, 634443, 634444)ÀÇ ¸®´º¾ó Á¦Ç°ÀÔ´Ï´Ù. ÀϺΠ±¸¼ºÇ°ÀÇ ¾÷±×·¹À̵å·Î ¾Æ·¡¿Í °°ÀÌ ¼º´ÉÀÌ °³¼±µÇ¾ú½À´Ï´Ù.
i. rRNA Á¦°Å ½Ã¾àÀÎ ZapR¢âÀÇ ¼º´ÉÀÌ Çâ»óµÇ¾î ¿øÄ¡ ¾Ê´Â read¸¦ ´õ¿í È¿°úÀûÀ¸·Î Á¦°ÅÇÕ´Ï´Ù.
ii. ÇÊ¿äÇÑ index¸¦ ¼±ÅÃÇÏ¿© Àû¿ëÇÒ ¼ö ÀÖµµ·Ï Á¦Ç°±¸¼º¿¡¼­ index¸¦ Á¦¿ÜÇÏ¿´½À´Ï´Ù. ÇÊ¿ä½Ã º°µµ ±¸¸Å ¹Ù¶ø´Ï´Ù.
>> ȣȯµÇ´Â Unique Dual Index kits (UDI kits)
ÀÚ¼¼ÇÑ ³»¿ëÀº ´ÙÄ«¶óÄÚ¸®¾Æ (02-2081-2510)·Î ¹®ÀÇ ÁÖ¼¼¿ä.

  • ºü¸£°í °£´ÜÇÑ ÇÁ·ÎÅäÄÝ - Strand Á¤º¸ (sense/antisense)¸¦ °¡Áø Illumina¢ç sequencing-ready library¸¦ 7½Ã°£¸¸¿¡ Á¦ÀÛ
  • Single cell ¼öÁرîÁö Àû¿ë °¡´É - 1-1,000 intact mammalian cell ȤÀº 10 pg - 10 ngÀÇ low/high-quality mammalian total RNA Àû¿ë
  • ³ôÀº ÀçÇö¼º°ú Á¤È®µµ·Î ºÐ¼® - Single cell ¶Ç´Â total RNA·ÎºÎÅÍ full-lengthÀÇ coding/non-coding transcript¸¦ ÀçÇö¼º°ú Á¤È®µµ ³ô°Ô ºÐ¼®
  • Single cell RNA-seqÀÇ gold standard SMART-Seq¢ç mRNA LP (with UMIs) (Code 634762 ¿Ü) ¼öÁØÀÇ ³ôÀº ¹Î°¨µµ
Á¦Ç°¼³¸í
SMART-Seq¢ç Total RNA Single Cell (ZapR¢â Mammalian)Àº random primer¸¦ »ç¿ëÇÏ¿© È¿°úÀûÀ¸·Î whole transcriptomeÀ» ºÐ¼®ÇÒ ¼ö ÀÖ´Â Á¦Ç°ÀÌ´Ù. ¶ÇÇÑ single cell ·¹º§¿¡¼­ nonpolyadenylated transcripts¸¦ ºÐ¼®ÇÒ ¼ö ÀÖ´Ù. Stranded sequencing library Á¦ÀÛÀ» À§ÇØ cDNA ÇÕ¼º ¹× amplification ½Ã¾à, ZapR ±â¹Ý ribosomal RNA (rRNA) Á¦°Å½Ã¾à ±×¸®°í Illumina¢ç¿ë library Á¦À۽þàÀÌ Æ÷ÇԵǾî ÀÖ´Ù.

ÀÌ Á¦Ç°Àº ´ç»çÀÇ µ¶ÀÚÀûÀÎ SMART (Switching Mechanism at 5¡¯ end of RNA Template) ±â¼ú¿¡ stranded RNA-seqÀ» À§ÇÑ °³¼±»çÇ×À» ´õÇØ ÇÁ·ÎÅäÄÝÀ» °£ÆíÈ­ÇÏ°í sequencing È¿À²À» ³ô¿´´Ù. ¶ÇÇÑ RT ´Ü°è¿¡¼­ random primer¸¦ »ç¿ëÇØ nonpolyadenylated transcript¸¦ Æ÷ÇÔÇÏ´Â full transcriptome ºÐ¼®ÀÌ °¡´ÉÇÏ´Ù.

Single cell ¼öÁرîÁö Àû¿ë °¡´ÉÇÑ »ç¿ëÀÚ Ä£È­ÀûÀÎ °£´ÜÇÑ ÇÁ·ÎÅäÄÝ°ú rRNA À¯·¡ cDNA Á¦°Å ½Ã¾à (ZapR¢â mammalian)Àº °í°¨µµ·Î ÀçÇö¼º ³ôÀ¸¸ç rRNA mappingÀÌ ³·Àº µ¥ÀÌÅ͸¦ ¾òÀ» ¼ö ÀÖ´Ù. µû¶ó¼­ º°µµÀÇ rRNA Á¦°Å kit ¾øÀÌ strand Á¤º¸ (sense /antisense)¸¦ °¡Áø sequencing library¸¦ °íÈ¿À²·Î Á¦ÀÛÇÒ ¼ö ÀÖ´Ù.

oligo dT primer¸¦ »ç¿ëÇÏ°íÀÚ ÇÑ´Ù¸é SMART-Seq¢ç mRNA LP (with UMIs) (Code 634762 ¿Ü) Á¦Ç°À» ÃßõÇÑ´Ù.


±×¸² 1. SMART-Seq¢ç Total RNA Single Cell (ZapR¢â Mammalian) ÇÁ·Î¼¼½º.
Àû¿ë
  • ±Ø¼Ò·® (1-1,000) mammalian cell ¹× total RNA (10 pg - 10 ng)¿¡¼­ 7½Ã°£¸¸¿¡ Illumina¢ç Ç÷§Æû¿ë sequencing library Á¦ÀÛ
  • Strand Á¤º¸¸¦ °¡Áø whole transcriptome sequencing
ÀÚ¼¼ÇÑ ±¸¼ºÀº CoA¸¦ Âü°íÇϼ¼¿ä.
RNA-Seq ·¯´×¼¾ÅÍ¿¡¼­ ´õ ¸¹Àº µ¥ÀÌÅÍ¿Í FAQ¸¦ È®ÀÎÇÏ½Ç ¼ö ÀÖ½À´Ï´Ù.

Keyword :

Stranded RNA-seq
>
(with UMI) FFPE, LCM, cell-free RNAµîÀÇ ±Ø¼Ò..
[New](+UMI) Pico input stranded RNA-Seq Kit
>
FFPE, LCM, cell-free RNAµîÀÇ ±Ø¼Ò·® low qual..
[New]Pico input stranded RNA-Seq Kit
>
´ÜÀϼ¼Æ÷ (Single cell)¿¡¼­ stranded RNA-Seq ..
[New]Single cell stranded RNA-Seq Kit
>
Mid Input Strand- Specific Total RNA-Seq
[New]Mid Input Stranded RNA-Seq Kit
>
High Input Strand- Specific Total RNA-Seq
[New]High Input Stranded RNA-Seq Kit
>
UDI, UMI Àû¿ëÀ¸·Î ´õ¿í Ưº°ÇØÁø
ÀÓ»ó»ùÇÃ ÃÖÀû Pico Input Mammalian(v3)
>
Picogram RNA·ÎºÎÅÍ Strand-specific illumina ..
ÀÓ»ó»ùÇÃ ÃÖÀû Pico Input Mammalian(v2)
>
´ÜÀϼ¼Æ÷ (single cell)ÀÇ stranded RNA-Seq li..
Single cell Stranded RNA-Seq Kit
>
High Input Strand- Specific Total RNA-Seq
High Input Stranded RNA-Seq Kit