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DNA Fragmentation Kit

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
6137
DNA Fragmentation Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
20 ȸ
239,200¿ø 
299,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
6137_e.v2408Da.pdf

Á¦Ç°¼³¸í
º» Á¦Ç°Àº ÃÊÀ½ÆÄ Æļâ ÀåÄ¡¿Í °°Àº Ư¼ö Àåºñ ¾øÀÌ, È¿¼Ò󸮸¦ ÅëÇØ gDNA¿Í °°Àº ±æÀÌ°¡ ±ä ÀÌÁß °¡´Ú DNA¸¦ ¹«ÀÛÀ§·Î ´ÜÆíÈ­ (random genomic DNA fragmentation)ÇÏ°í ÆòÈ°¸»´Ü (blunt end)À¸·Î ¸¸µå´Â Á¦Ç°ÀÌ´Ù. ÆòÈ° ¸»´ÜÀ» °¡Áø DNA ´ÜÆíÀº ±×´ë·Î ÆòÈ° ¸»´ÜÀ» °¡Áø vector (blunt end vector)¿¡ ligation µÉ ¼ö ÀÖ°í, ÆòÈ° ¸»´ÜÀÌ ÇÊ¿äÇÏÁö ¾ÊÀº °æ¿ì ´ÜÆíÈ­ °úÁ¤ ±îÁö¸¸ ¼öÇàÇÒ ¼öµµ ÀÖ´Ù. ¶ÇÇÑ ¸ÞÆ¿È­µÈ DNAÀÇ ³óÃà, DNA »ùÇÃÀÇ sequencing¿¡ Àü󸮿¡¼­ »ç¿ëÇÒ ¼ö ÀÖ´Ù.
³»¿ë (20ȸ, 20 §¡/ȸ )

Enzyme - 1

20 §¡

Dilution Buffer -1

1,040 §¡

A solution

20 §¡

B solution

50 §¡

Stop solution

400 §¡

150mM MgCl2

20 §¡

Dilution Buffer - 2

200 §¡

Enzyme - 2

20 §¡

0.5 M EDTA

50 §¡

dH2O

10 X 1 §¢

º¸Á¸ -20¡É
- A solution, B solution, 150mM MgCl2, 0.5 M EDTA, dH2O´Â 4¡É¿¡¼­µµ º¸°ü °¡´É
¿ëµµ
- gDNA ´ÜÆíÈ­
- ÀÌÁß °¡´Ú DNA ´ÜÆíÈ­
´ÜÆíÈ­ ¹ÝÀÀ
- ±ÇÀå ¹ÝÀÀ¾×·®
gDNA 100 ng ÀÌÇÏ: 10 §¡ ¹ÝÀÀ¾× ½Ã½ºÅÛ
gDNA 100 ng ~ 1 §¶: 20§¡ ¹ÝÀÀ¾× ½Ã½ºÅÛ
¡Ø 1 §¶ ÀÌ»óÀÏ °æ¿ì 1 §¶ / 20 §¡ ¸¦ ±âÁØÀ¸·Î ¹ÝÀÀ tube ¼ö¸¦ ´Ã¸°´Ù.
¶Ç´Â ÃÖ´ë 5 §¶ / 100 §¡±îÁö ¹ÝÀÀ¾×À» ´Ã¸°´Ù.

- ´ÜÆíÈ­ È®ÀÎ
1.5% Agarose L03¿¡¼­ gDNA 200~250 ng¿¡ ÇØ´çÇÏ´Â ¾çÀ» Àü±â¿µµ¿ ÈÄ È®ÀÎ

Keyword :

NGS Sample preparation
>
Human, mouse, rat total RNA·ÎºÎÅÍ rRNA ¹× mt..
rRNA Á¦°Å½Ã¾à RiboGone¢â-Mammalian
>
Magnetic bead-based DNA purification & size ..
Magnetic Separator-PCR Strip