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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > Whole Genome Amplification (WGA) > PicoPLEX¢ç Single Cell WGA Kit v3
SNV, CNV °ËÃâ °¡´ÉÇÑ Whole genome amplification (WGA) from single cells

PicoPLEX¢ç Single Cell WGA Kit v3

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
R300718
PicoPLEX¢ç Single Cell WGA Kit v3
°ü·ÃÇмú ±¸¸ÅÇϱâ
24 ȸ
1,136,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x107472
Clontech
R300722
PicoPLEX¢ç Single Cell WGA Kit v3
°ü·ÃÇмú ±¸¸ÅÇϱâ
96 ȸ
3,287,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
R300723
PicoPLEX¢ç Single Cell WGA Kit v3
°ü·ÃÇмú ±¸¸ÅÇϱâ
480 ȸ (R300722 x 5)
14,792,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

* º» Á¦Ç°Àº ±âÁ¸ PicoPLEX¢ç Single Cell WGA Kit (Code R300671)ÀÇ ¾÷±×·¹ÀÌµå ¹öÀü Á¦Ç°À¸·Î, CNV´Â ¹°·Ð SNVÀÇ °ËÃâ±îÁö °¡´ÉÇÑ ÃÖ°í ¹Î°¨µµ ¼öÁØÀÇ Á¦Ç°ÀÔ´Ï´Ù.

  • Single cells ȤÀº 15 pg of DNA ¼öÁØ¿¡¼­ ÀçÇö¼º ÀÖ´Â °á°ú
  • ¿ùµîÇÑ Allele representation
  • ÀÚµ¿È­ ±â±â Àû¿ë¿¡ ÃÖÀû
  • ¸ðµç ½ÇÇè Àû¿ë¿¡¼­ ÆíÇ⼺ ¾ø´Â °á°ú (e.g., microarray and PCR)
Á¦Ç°¼³¸í
PicoPLEX Single Cell WGA Kit v3´Â single-tube protocol¸¦ ÀÌ¿ëÇÏ¿©, 1-10°³ÀÇ ¼¼Æ÷ ȤÀº µ¿µîÇÑ pg ¼öÁØÀÇ gDNA·ÎºÎÅÍ ÀçÇö¼º ÀÖ´Â ÁõÆøÀÌ °¡´ÉÇÏ´Ù. Cell lysis, whole genome preamplification, ÁõÆø °úÁ¤ÀÌ 2.5½Ã°£ ³»¿¡ ÁøÇàµÇ¸ç, ÃÖ¼ÒÈ­µÈ ¿¡·¯¹ß»ý·ü·Î »ùÇÃÀ» 2 ¥ìg ¼öÁØÀ¸·Î ÁõÆøÇÑ´Ù. ¶ÇÇÑ High-fidelity È¿¼Ò¸¦ µµÀÔÇÔÀ¸·Î½á, ±âÁ¸ Á¦Ç°¿¡¼­ °¡´ÉÇÑ CNV »Ó ¾Æ´Ï¶ó, SNV °ËÃâ¿¡µµ È°¿ëµÉ ¼ö ÀÖ´Ù.
»ùÇÃ
- Mammalian cells (1-10 cells)
- Bacterial cells (1,000-10,000 cells)
- Isolated DNA (15-60 pg of human DNA)
- Sorted chromosomes
- Intact or fragmented, single- or double-stranded DNA
Àû¿ë
- Copy number variation (CNV) analysis using NGS, oligonucleotide aCGH, BAC aCGH, and qPCR
- Target enrichment or amplicon enrichment for mutation detection
- SNP genotyping


±×¸² 1.  PicoPLEX¢ç WGA±â¼úÀÇ ¿ø¸®
Cells are lysed, quasi-random primers pre-amplify the DNA selectively, and a final PCR amplification adds the Illumina barcodes. ( PicoPLEX¢ç ±â¼ú °³¿ä º¸±â)


±×¸² 2. PicoPLEX WGA v3 workflow overview
Steps involved in PicoPLEX Single Cell WGA Kit v3 sample preparation starting from a single cell.
ÀÚ¼¼ÇÑ ±¸¼ºÀº CoA¸¦ Âü°íÇϼ¼¿ä.

Keyword : Sngle cell,´ÜÀϼ¼Æ÷,½Ì±Û¼¿,single cell analysis,single cell NGS,Ultra low input,WGA,Whole genome amplification,NGS,PGS,PGD,PGA,žƻêÀü°Ë»ç,»êÀü°Ë»ç,DNA-seq,CNV,SNV,SNP,Genotyping,array,variation,DNA variation

Whole Genome Amplification (WGA)
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SNV, CNV °ËÃâ °¡´ÉÇÑ Whole genome amplificat..
PicoPLEX¢ç Single Cell WGA Kit v3
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Whole genome amplification (WGA) from single..
PicoPLEX¢ç Single Cell WGA Kit