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NGS °ü·Ã
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Whole Genome Amplification (WGA)
> PicoPLEX¢ç Single Cell WGA Kit v3
SNV, CNV °ËÃâ °¡´ÉÇÑ Whole genome amplification (WGA) from single cells
PicoPLEX¢ç Single Cell WGA Kit v3
Á¦Á¶»ç
Á¦Ç°ÄÚµå
Á¦Ç°¸í
¿ë·®
°¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í
»ç¿ëÀڸŴº¾ó
Clontech
R300718
PicoPLEX¢ç Single Cell WGA Kit v3
24 ȸ
1,136,000¿ø
Clontech
R300722
PicoPLEX¢ç Single Cell WGA Kit v3
96 ȸ
3,287,000¿ø
Clontech
R300723
PicoPLEX¢ç Single Cell WGA Kit v3
480 ȸ (R300722 x 5)
14,792,000¿ø
Product index
PGT-A
Embgenix¢â PGT-A Kit (..
SMARTer RNA-Seq °³¿ä
[±â¼ú°³¿ä] SMARTer Tec..
RNA-Seq ¼±Åð¡À̵å
NGS ¼±Åð¡À̵å (NGS ±â..
RNA-Seq Á¦Ç°¸íÀÌ ÀÌ·¸..
mRNA-Seq (Single cell)
[New]Single cell mRNA-..
[New]Ultra low input m..
[New]High throughput, ..
Single cell mRNA-Seq K..
Ultra low input mRNA-S..
High throughput, Autom..
Ultra low input 3¡¯end..
Stranded RNA-seq
[New](+UMI) Pico i..
[New]Pico input strand..
[New]Single cell stran..
[New]Mid Input Strande..
[New]High Input Strand..
Total RNA-Seq Kit (FFP..
ÀÓ»ó»ùÇÃ ÃÖÀû Pico Inp..
ÀÓ»ó»ùÇÃ ÃÖÀû Pico Inp..
Single cell Stranded R..
Mid Input Stranded RNA..
High Input Stranded RN..
Small RNA-Seq
Small RNA-Seq Kit
Immune-Seq
[¼±Åð¡À̵å] Immune-se..
[New]UMI Àû¿ë Human TC..
[New]UMI Àû¿ë Human BC..
[New]UMI Àû¿ë Mouse TC..
[New]UMI Àû¿ë Mouse BC..
UMI Àû¿ë Human TCR pro..
Human BCR IgG IgM H/K/..
Mouse TCR a/b Profilin..
Mouse BCR IgG H/K/L Pr..
±âŸ RNA-Seq
Full length cDNA ÇÕ¼º
Target RNA Capture for..
DNA-seq °³¿ä
[±â¼ú°³¿ä] ThruPLEX & ..
DNA-Seq ¼±Åð¡À̵å
ChIP-Seq / Meth-Seq
50pg ChIP DNA-seq Kit
DNA SMART ChIP-Seq Kit
Meth-Seq DNA Enrichmen..
Micrococcal Nuclease
Low Input DNA-Seq
ThruPLEX¢ç DNA-seq Kit
ThruPLEX¢ç Plasma-seq ..
PicoPLEX¢ç Single Cell..
High input DNA-seq
ThruPLEX¢ç DNA-Seq HV
ThruPLEX¢ç Tag-Seq HV
Single cell DNA-Seq
PicoPLEX¢ç Single Cell..
Whole Genome Amplification..
[±â¼ú°³¿ä] ThruPLEX & ..
PicoPLEX¢ç Single Cell..
PicoPLEX¢ç Single Cell..
Index Kit
ThruPLEX, PicoPLEX¿ë I..
Stranded-SMART kit¿ë U..
SMART-PLUS kit¿ë UDI (..
Unique Dual Index Kits
NGS Library Á¤·®
Library Quantification..
NGS Sample preparation
rRNA Á¦°Å½Ã¾à RiboGone..
Magnetic Separator-PCR..
DNA Fragmentation Kit
NGS ºÐ¼®¿ë PCR È¿¼Ò
SeqAmp¢â DNA Polymeras..
PrimeSTAR¢ç GXL DNA Po..
PreAmp Master Mix
NGS °ü·Ã Àåºñ
ICELL8 cx Technical No..
ICELL8¢ç cx Single-Cel..
ICELL8 cx 3 Differenti..
ICELL8¢ç cx Single-Cel..
ICELL8 cx Human TCR Pr..
ICELL8¢ç cx blank chip
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
[Legacy] NGS Products
(Legacy) Human TCR a/b..
(Legacy) PicoPLEX¢ç WG..
* º» Á¦Ç°Àº ±âÁ¸ PicoPLEX
¢ç
Single Cell WGA Kit (Code R300671)ÀÇ ¾÷±×·¹ÀÌµå ¹öÀü Á¦Ç°À¸·Î, CNV´Â ¹°·Ð SNVÀÇ °ËÃâ±îÁö °¡´ÉÇÑ ÃÖ°í ¹Î°¨µµ ¼öÁØÀÇ Á¦Ç°ÀÔ´Ï´Ù.
Single cells ȤÀº 15 pg of DNA ¼öÁØ¿¡¼ ÀçÇö¼º ÀÖ´Â °á°ú
¿ùµîÇÑ Allele representation
ÀÚµ¿È ±â±â Àû¿ë¿¡ ÃÖÀû
¸ðµç ½ÇÇè Àû¿ë¿¡¼ ÆíÇ⼺ ¾ø´Â °á°ú (e.g., microarray and PCR)
Á¦Ç°¼³¸í
PicoPLEX Single Cell WGA Kit v3´Â single-tube protocol¸¦ ÀÌ¿ëÇÏ¿©, 1-10°³ÀÇ ¼¼Æ÷ ȤÀº µ¿µîÇÑ pg ¼öÁØÀÇ gDNA·ÎºÎÅÍ ÀçÇö¼º ÀÖ´Â ÁõÆøÀÌ °¡´ÉÇÏ´Ù. Cell lysis, whole genome preamplification, ÁõÆø °úÁ¤ÀÌ 2.5½Ã°£ ³»¿¡ ÁøÇàµÇ¸ç, ÃÖ¼ÒÈµÈ ¿¡·¯¹ß»ý·ü·Î »ùÇÃÀ» 2 ¥ìg ¼öÁØÀ¸·Î ÁõÆøÇÑ´Ù. ¶ÇÇÑ High-fidelity È¿¼Ò¸¦ µµÀÔÇÔÀ¸·Î½á, ±âÁ¸ Á¦Ç°¿¡¼ °¡´ÉÇÑ CNV »Ó ¾Æ´Ï¶ó, SNV °ËÃâ¿¡µµ È°¿ëµÉ ¼ö ÀÖ´Ù.
»ùÇÃ
- Mammalian cells (1-10 cells)
- Bacterial cells (1,000-10,000 cells)
- Isolated DNA (15-60 pg of human DNA)
- Sorted chromosomes
- Intact or fragmented, single- or double-stranded DNA
Àû¿ë
- Copy number variation (CNV) analysis using NGS, oligonucleotide aCGH, BAC aCGH, and qPCR
- Target enrichment or amplicon enrichment for mutation detection
- SNP genotyping
±×¸² 1. PicoPLEX
¢ç
WGA±â¼úÀÇ ¿ø¸®
Cells are lysed, quasi-random primers pre-amplify the DNA selectively, and a final PCR amplification adds the Illumina barcodes. (
PicoPLEX
¢ç
±â¼ú °³¿ä º¸±â
)
±×¸² 2. PicoPLEX WGA v3 workflow overview
Steps involved in PicoPLEX Single Cell WGA Kit v3 sample preparation starting from a single cell.
ÀÚ¼¼ÇÑ ±¸¼ºÀº CoA¸¦ Âü°íÇϼ¼¿ä.
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Keyword : Sngle cell,´ÜÀϼ¼Æ÷,½Ì±Û¼¿,single cell analysis,single cell NGS,Ultra low input,WGA,Whole genome amplification,NGS,PGS,PGD,PGA,žƻêÀü°Ë»ç,»êÀü°Ë»ç,DNA-seq,CNV,SNV,SNP,Genotyping,array,variation,DNA variation
Whole Genome Amplification (WGA)
[±â¼ú°³¿ä] ThruPLEX & PicoPLEX
SNV, CNV °ËÃâ °¡´ÉÇÑ Whole genome amplificat..
PicoPLEX¢ç Single Cell WGA Kit v3
Whole genome amplification (WGA) from single..
PicoPLEX¢ç Single Cell WGA Kit