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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > NGS Library Á¤·® > Library Quantification Kit (for Illumina)
Illumina¿ë NGS Library Á¤·® Å°Æ®

Library Quantification Kit (for Illumina)

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Clontech
638324
Library Quantification Kit
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500 ȸ
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1,164,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32789
Clontech
638325
DNA Standards for Library Quantification
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50 ȸ
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  • Highly sensitive quantification of Illumina sequencing libraries - the qPCR-based assay can measure even low concentration libraries
  • Allows specific quantification of only DNA molecules bound to adapters
  • Reduces variability in cluster density to improve sequencing results - use prior to sequencing to ensure optimal loading concentration
  • Complete kit - includes qPCR primers and reagents, and DNA standards for absolute quantification using a standard curve
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Illumina NGS library quantification by qPCR
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High-qualityÀÇ Illumina next-generation sequencing µ¥ÀÌÅ͸¦ ¾ò±â À§ÇÏ¿©, ÀûÀýÇÑ ¾çÀÇ library DNA¸¦ ÀÌ¿ëÇÏ´Â °ÍÀÌ ÇʼöÀûÀÌ´Ù. Library DNAÀÇ ¾çÀÌ ºÎÁ·Çϸé cluster ¹Ðµµ (cluster density)°¡ ³·¾ÆÁ® ½ÃÄö½Ì ¼öÀ²(sequencing yield)ÀÌ ³·¾ÆÁø´Ù. ¹Ý¸é, Library DNAÀÇ ¾çÀÌ ³Ê¹« ¸¹À¸¸é Cluster ¹Ðµµ°¡ ³ô¾ÆÁ® High-qualityÀÇ µ¥ÀÌÅ͸¦ ¾òÀ» ¼ö ¾ø´Ù. Àü±â¿µµ¿(Electrophoresis)À̳ª ºÐ±¤ ±¤µµ ÃøÁ¤(spectrophotometry)°ú °°Àº ±âÁ¸ÀÇ NGS Library Á¤·® ¹æ¹ýÀº sensitivity°¡ ³·°í, adaptor-bound DNA¿¡ ´ëÇÏ¿© ºñƯÀÌÀû ÀϻӸ¸ ¾Æ´Ï¶ó, ¸¹Àº ¾çÀÇ Library DNA°¡ ÇÊ¿äÇÏ´Ù.
Library Quantification KitÀº °í°¨µµÀÇ qPCR ±â¹ÝÀÇ Á¤·®¹ýÀ» »ç¿ëÇϱ⠶§¹®¿¡ IlluminaÀÇ NGS ¹ÝÀÀ¿¡ »ç¿ëµÇ´Â adaptor sequence¸¦ ƯÀÌÀûÀ¸·Î °ËÃâÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ, »ùÇà ³»¿¡ adaptor°¡ ºÎ°¡µÇÁö ¾ÊÀº DNAºÐÀÚ°¡ ÀÖ´õ¶óµµ sequencing libraryÀÇ ³óµµ¸¦ ÃøÁ¤ °¡´ÉÇϸç, library Á¦ÀÛ °úÁ¤¿¡¼­ adaptor ligationÀ» È®ÀÎÇÏ´Â µ¥ »ç¿ëÇÒ ¼öµµ ÀÖ´Ù.

º» Á¦Ç°¿¡´Â standard curve ÀÛ¼ºÀ» À§ÇÑ DNA Standard°¡ Æ÷ÇԵǾîÀÖ¾î, library DNAÀÇ Àý´ëÁ¤·®ÀÌ °¡´ÉÇϸç, DNA standards for Library Quantification(Code 638325)´Â º°µµ·Î ±¸¸Å °¡´ÉÇÏ´Ù.

±×¸² 1. A typical RNA-seq workflow. Generating sufficient amounts of library DNA and loading an appropriate amount on the flow cell is essential for obtaining high-quality NGS data. The Library Quantification Kit, a qPCR-based quantification method that targets Illumina¡¯s adapter sequences, can be used to measure library concentration and ensure optimal loading concentration


±×¸² 2. Lot-to-lot variation for DNA Standards. Quantification of three lots of pre-diluted DNA Standards (0.01, 0.1, 1, and 10 pM) was performed by real-time PCR using Terra qPCR Direct SYBR Premix following the protocol in the Library Quantification Kit user manual. All reactions were performed in triplicate, and the resulting Ct (SDM) values for the triplicates were averaged. The variation between lots was <5%.
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Library Quantification Kit (Code 638324)
  • Terra qPCR Direct SYBR Premix (Code 638319)
  • Primer Mix Kit
  • DNA Standards for Library Quantification (Code 638325)
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Terra qPCR Direct SYBR Premix: -70¡É (À¶ÇØ ÈÄ, 4¡É¿¡¼­ 6°³¿ù º¸Á¸)
±âŸ: -20¡É

Keyword : Library Á¤·®,Illumina ngs,ÀÏ·ç¹Ì³ª Á¤·®,illumina,illumina qpcr

NGS Library Á¤·®
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Illumina¿ë NGS Library Á¤·® Å°Æ®
Library Quantification Kit (for Illumina)