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NGS °ü·Ã
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Whole Genome Amplification (WGA)
> PicoPLEX¢ç Single Cell WGA Kit
Whole genome amplification (WGA) from single cells
PicoPLEX¢ç Single Cell WGA Kit
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Clontech
R300671
PicoPLEX¢ç Single Cell WGA Kit
24ȸ
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Clontech
R300672
PicoPLEX¢ç Single Cell WGA Kit
96ȸ
°¡°Ý¹®ÀÇ
Clontech
R300673
PicoPLEX¢ç Single Cell WGA Kit
480ȸ
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Product index
SMARTer RNA-Seq °³¿ä
[±â¼ú°³¿ä] SMARTer Tec..
RNA-Seq ¼±Åð¡À̵å
NGS ¼±Åð¡À̵å (NGS ±â..
[FAQ] RNA-seq
Cancer ¿¬±¸ °¡À̵å
Cell-free DNA/RNA °¡ÀÌ..
SMARTer RNA-seqÀÇ HOT ..
mRNA-Seq (Single cell)
[Cancer Research] Canc..
Single cell-seq °¡À̵å
Single cell SMART-seq..
Single cell RNA-seq¿¡ ..
±Ø¼Ò·® »ùÇÃ(pg ¼öÁØ)À»..
´Ù·®ÀÇ ±Ø¼Ò·® »ùÇÃÀ» ..
±Ø¼Ò·® mRNAÀÇ 3¡¯ ¸»´Ü..
Stranded RNA-seq
Single cell Stranded K..
[NEW] Pico Input Mamma..
Pico Input Mammalian (..
Low Input Mammalian
HI Mammalian
Stranded RNA-Seq Kit
Total RNA-Seq Kit (FFP..
Small RNA-Seq
[Cancer Research] Canc..
Small RNA-Seq Kit
Immune-Seq
[¼±Åð¡À̵å] Immune-se..
[Cancer Research] Immu..
[Version 2] Human TCR ..
Human TCR a/b Profilin..
Human BCR IgG IgM H/K/..
Mouse TCR a/b Profilin..
Mouse BCR IgG H/K/L Pr..
±âŸ RNA-Seq
Full length cDNA ÇÕ¼º
Target RNA Capture for..
DNA-seq °³¿ä
[±â¼ú°³¿ä] ThruPLEX & ..
DNA-Seq ¼±Åð¡À̵å
Cell-free DNA/RNA °¡ÀÌ..
Single cell-seq °¡À̵å
[FAQ] DNA-Seq
[Àû¿ë] Overview_ThruPL..
[Cancer Research] Canc..
DNA-seqÀÇ ´Ù¾çÇÑ ÃֽŠ..
ChIP-Seq / Meth-Seq
[Àû¿ë] ThruPLEX¸¦ ÀÌ¿ë..
[³í¹®] ThruPLEX¢ç for ..
50pg ChIP DNA-seq Kit
DNA SMART ChIP-Seq Kit
Meth-Seq DNA Enrichmen..
Micrococcal Nuclease
Low Input DNA-Seq
[Àû¿ë] ThruPLEX¸¦ ÀÌ¿ë..
[Àû¿ë] ThruPLEX¸¦ ÀÌ¿ë..
ThruPLEX¢ç DNA-seq Kit
ThruPLEX¢ç Plasma-seq ..
PicoPLEX¢ç Single Cell..
High input DNA-seq
ThruPLEX¢ç DNA-Seq HV
ThruPLEX¢ç Tag-Seq HV
Single cell DNA-Seq
[Cancer Research] Canc..
Single cell-seq °¡À̵å
PicoPLEX¢ç Single Cell..
Whole Genome Amplification..
[±â¼ú°³¿ä] ThruPLEX & ..
[Cancer Research] HLA ..
[³í¹®] PicoPLEX¢ç ±â¼ú..
PicoPLEX¢ç Single Cell..
PicoPLEX¢ç Single Cell..
Index Kit
[FAQ] Library index
ThruPLEX, PicoPLEX¿ë I..
Stranded-SMART kit¿ë U..
SMART-PLUS kit¿ë UDI (..
NGS Library Á¤·®
Library Quantification..
NGS Sample preparation
rRNA Á¦°Å½Ã¾à RiboGone..
Magnetic Separator-PCR..
DNA Fragmentation Kit
NGS ºÐ¼®¿ë PCR È¿¼Ò
SeqAmp¢â DNA Polymeras..
PrimeSTAR¢ç GXL DNA Po..
PreAmp Master Mix
NGS °ü·Ã Àåºñ
ICELL8 cx Citation
ICELL8 cx Technical No..
ICELL8¢ç cx Single-Cel..
ICELL8 cx 3 Differenti..
ICELL8¢ç cx Single-Cel..
ICELL8 cx Human TCR Pr..
ICELL8¢ç cx blank chip
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
Apollo¢â Library Prep ..
[Legacy] NGS Products
(Legacy) SMARTer Ultra..
(Legacy) SMARTer¢ç Str..
(Legacy) PicoPLEX¢ç DN..
(Legacy) ThruPLEX¢ç Pl..
(Legacy) ThruPLEX¢ç DN..
(Legacy) PicoPLEX¢ç WG..
(Legacy) ThruPLEX¢ç Ta..
* Àá±ñ, ù ±¸¸ÅÀΰ¡¿ä? ´ÙÄ«¶óÄÚ¸®¾Æ °í°´Áö¿ø¼¾ÅÍ (Tel. 02-2081-2510)·Î ¿¬¶ôÁÖ¼¼¿ä!
* º» Á¦Ç°Àº ±âÁ¸ PicoPLEX
¢ç
WGA Kit (Code R30050)ÀÇ ¾÷±×·¹ÀÌµå ¹öÀü Á¦Ç°ÀÔ´Ï´Ù.
Reproducible results from single cells (or <15 pg of DNA)
Superior reproducibility of allele representation
Easy to use and automate
Unambiguous results at all resolutions (e.g., microarray and PCR)
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PicoPLEX
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Single Cell WGA Kit´Â ´ÜÀϼ¼Æ÷ (single cell)·ÎºÎÅÍ ³ôÀº ÀçÇö¼º°ú Á¤È®µµ·Î Genomic DNA¸¦ ÁõÆø °¡´ÉÇÑ Á¦Ç°ÀÌ´Ù. º» Á¦Ç°ÀÇ ±âº»ÀÌ µÇ´Â °íÀ¯ÀÇ ±â¼ú(ƯÇã ±â¼ú),
PicoPLEX
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񃬣
Àº single copy genomic DNAÀÇ ÁõÆø¿¡ ÃÖÀûȵǾî ÀÖÀ¸¸ç ÇϳªÀÇ tube¿¡¼ ¹ÝÀÀÇÏ´Â single tube reactionÀ¸·Î »ç¿ëÇÏ±â Æí¸®Çϰí, ¿¡·¯ ¹× ¹é±×¶ó¿îµå¸¦ ÃÖ¼ÒÈÇÏ¿© Çâ»óµÈ °á°ú¸¦ µµÃâÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°Àº single cell (1 - 10 cells)»Ó ¾Æ´Ï¶ó, ÃßÃâÇÑ ±Ø¼Ò·®(6 - 50 pg)ÀÇ gDNA·ÎºÎÅÍ Whole Genome Amplification (WGA)¸¦ ÁøÇàÇÒ ¼ö ÀÖÀ¸¸ç, ÁõÆøÇÑ DNA´Â PCRÀº ¹°·Ð array CGH analysis¿¡µµ Àû¿ëÇÒ ¼ö ÀÖ´Ù.
PicoPLEX
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WGA Kit˼
in vitro
fertilization(IVF, ü¿Ü¼öÁ¤)½Ã pre-implantation genetic screening and diagnostics (PGS/PGD)¿Í °°Àº °Ë»ç ½Ã À¯¿ëÇÏ°Ô »ç¿ëµÇ°í ÀÖ´Ù.
±×¸² 1. PicoPLEX
¢ç
WGA±â¼úÀÇ ¿ø¸®
Cells are lysed, quasi-random primers pre-amplify the DNA selectively, and a final PCR amplification adds the Illumina barcodes. (
PicoPLEX
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±â¼ú °³¿ä º¸±â
)
±×¸² 2. Reproducible amplification
Flow-sorted cancer cells were amplified with PicoPLEX single cell WGA technology. Each of the cells (blue lines) amplified at the same rate and resulted in a similar, predictable yield. A pooled sample of five cells (red line) represents a positive control. Samples containing 0 cells (green lines, no template control) show very low background.
±×¸² 3. Reproducible CNVs
Single-blastomere biopsies were amplified using PicoPLEX single cell WGA technology, labeled and hybridized to BlueGnome¡¯s 24 Sure arrays at Genesis Genetics Institute. Note the clear indiciation of CNVs. In 2011 ESHRE clinical trials confirmed accuracy of karyotyping using PicoPLEX technology.
±×¸² 4. Greater reliability
Locus-specific qPCR was used to quantify 48 loci in independent single-cell libraries. Data shown compares results of DNA from two individual samples amplified with (left to right) PicoPLEX single cell WGA technology, GenomePLEX, and GehomiPhi using 10pg of DNA. More than 90% of the product from the PicoPLEX single cell WGA technology produced identifiable, highly reproducible human sequences.
Applications
Copy Number Variation (CNV) analysis using NGS, oligonucleotide aCGH, BAC aCGH, and qPCR
Target enrichment or amplicon enrichment for mutation detection
SNP Genotyping
±¸¼ºÇ° (ÀÚ¼¼ÇÑ ³»¿ëÀº CoA¸¦ ÂüÁ¶Çϼ¼¿ä)
- Cell Extraction Buffer
- Extraction Enzyme Dilution Buffer
- Cell Extraction Enzyme
- Pre-Amp W Buffer
- Pre-Amp W Enzyme
- Amplification Buffer
- Amplification Enzyme
- Nuclease-Free Water
Keyword : Sngle cell,´ÜÀϼ¼Æ÷,½Ì±Û¼¿,single cell analysis,single cell NGS,Ultra low input,WGA,Whole genome amplification,NGS,PGS,PGD,PGA,žƻêÀü°Ë»ç,»êÀü°Ë»ç,DNA-seq,CNV,SNV,SNP,Genotyping,array,variation,DNA variation
Whole Genome Amplification (WGA)
[±â¼ú°³¿ä] ThruPLEX & PicoPLEX
[Cancer Research] HLA typing
[³í¹®] PicoPLEX¢ç ±â¼ú°ú ´Ù¾çÇÑ applications
SNV, CNV °ËÃâ °¡´ÉÇÑ Whole genome amplificat..
PicoPLEX¢ç Single Cell WGA Kit v3
Whole genome amplification (WGA) from single..
PicoPLEX¢ç Single Cell WGA Kit