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Total RNA-Seq Kit (FFPE, LCM)

제조사	제품코드	제품명	용량	가격 (부가세별도)	비고	사용자매뉴얼
Clontech	634938	SMARTer® Universal Low Input RNA Kit for Sequencing	10 회 (Package)	2,184,000원		, ,
Clontech	634940	SMARTer® Universal Low Input RNA Kit for Sequencing	25 회 (Package)	4,678,000원		, ,

Product index

PGT-A
- Embgenix™ PGT-A Kit (..
SMARTer RNA-Seq 개요
mRNA-Seq (Single cell)
Stranded RNA-seq
Small RNA-Seq
- Small RNA-Seq Kit
Immune-Seq
기타 RNA-Seq
- Full length cDNA 합성
- Target RNA Capture for..
DNA-seq 개요
- [기술개요] ThruPLEX & ..
- DNA-Seq 선택가이드
ChIP-Seq / Meth-Seq
Low Input DNA-Seq
High input DNA-seq
- ThruPLEX® DNA-Seq HV
- ThruPLEX® Tag-Seq HV
Single cell DNA-Seq
- PicoPLEX® Single Cell..
Whole Genome Amplification..
Index Kit
NGS Library 정량
- Library Quantification..
NGS Sample preparation
NGS 분석용 PCR 효소
NGS 관련 장비
[Legacy] NGS Products
- (Legacy) Human TCR a/b..
- (Legacy) PicoPLEX® WG..

제품 설명

SMARTer Universal Low Input RNA Kit for Sequencing은 낮은 RIN 값을 가지는 소량의 RNA 뿐만 아니라 non-polyA mRNA (Bacteria sample)로부터 간단하고 효과적으로 RNA-seq에 적용 가능한 cDNA를 합성 가능하다. 이 Kit은 불과 200 pg의 rRNA-depleted RNA (2-10 ng의 total RNA로 준비)로부터 NGS에 적용할 수 있는 cDNA를 조제할 수 있다. 특히 SMARTer Universal Low Input RNA Kit for Sequencing은 FFPE나 LCM (Laser Captured Materials) 샘플에 적용할 수 있도록 고안 되어 있으며 기존의 방법에 비해 body coverage, 재현성, mappability 성능이 좋다.

그림 1. NGS Workflow for the SMARTer Universal Low Input RNA Kit for Sequencing.

Features

Sensitivity to handle low-input samples-Works with as little as 200 pg of input RNA rRNA-depleted material (derived from 2-10 ng of total RNA)
Efficient results from compromised samples-Ideal solution for FFPE samples
RNA-seq data quality-Consistent RNA-seq data across all input ranges (200 pg-10 ng)
- Mappability > 80%
- Reproducibility >0.98
- ERCC correlation > 0.99
- Number of genes > 15,000
Complete kit includes RT and PCR enzymes

Applications

Generate cDNA for RNA-seq from very low amounts of total RNA (2-10 ng) from FFPE or LCM samples
Generate cDNA for NGS from very low amounts of total RNA (2-10 ng) that are:
- Compromised (obtained from FFPE or LCM samples)
- Have low RIN values
- Are from sources that do not have polyadenylated mRNA

RNA-seq Solutions from Clontech

Product	SMART-Seq® v4 Ultra® Low Input RNA Kit for Sequencing	SMARTer® Universal Low Input RNA Kit
Code	634888	634938
size	12회	10회
Application	* Transcriptome analysis (mRNA-seq) * Ultra Low input mRNA-seq * Single cell mRNA-seq	* mRNA-seq, Total RNA-seq, Non-coding RNA-seq * Low input RNA-seq
Input material	* 10pg - 10ng input of mRNA * High quality total RNA (RIN>8) * Single cell * Few cells (1 - 1,000 cells)	* 200pg - 10ng input of rRNA depleted RNA * Degraded samples (fragmented RNA) (RIN 2-3) - FFPE, Laser capture microscopy(LCM) * Non polyadenylated RNA (Bacteria)
Technology	* Oligo dT Priming * Works both isolated RNA and directly on cells	* Random priming * Requires ribosomal RNA removal * Requires RNA isolation

Keyword :

Stranded RNA-seq

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