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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > NGS °ü·Ã Àåºñ > ICELL8 cx Human TCR Profiling Kit

ICELL8 cx Human TCR Profiling Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
640197
ICELL8¢ç cx Loading Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 Rxn
°¡°Ý¹®ÀÇ   ICELL8 cx Human TCR ab Profiling User Manual_030920.pdf
Takara
640048
ICELL8¢ç Collection Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 Kit
°¡°Ý¹®ÀÇ  
Takara
640200
ICELL8¢ç cx TCR Chip
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 chip
°¡°Ý¹®ÀÇ   ICELL8 cx Human TCR ab Profiling User Manual_030920.pdf
Takara
640018
MSND 384-Well Source Plate and Seals (430-000025)
°ü·ÃÇмú ±¸¸ÅÇϱâ
20/Pack
°¡°Ý¹®ÀÇ  
Takara
640179
ICELL8¢ç Human TCR a/b Profiling - Indexing Primer Set
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 chip
°¡°Ý¹®ÀÇ   ICELL8 cx Human TCR ab Profiling User Manual_030920.pdf
Takara
640180
ICELL8¢ç Human TCR a/b Profiling - Indexing Primer Set
°ü·ÃÇмú ±¸¸ÅÇϱâ
5 chips
°¡°Ý¹®ÀÇ  
Takara
640181
ICELL8¢ç Human TCR a/b Profiling - Indexing Primer Set
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 chips
°¡°Ý¹®ÀÇ  
Takara
640182
ICELL8¢ç Human TCR a/b Profiling Reagent Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 chip
(Package)
°¡°Ý¹®ÀÇ   ICELL8 cx Human TCR ab Profiling User Manual_030920.pdf

  • ICELL8 cx½Ã½ºÅÛÀ¸·Î ºÐ¸®µÈ ¼ö¹é°³ÀÇ ´ÜÀÏ Human T¼¼Æ÷ÀÇ¥á-chain¹× ¥â-chainÀÇ V(D)J variable region ÀÇ sequence¿Í pairing Á¤º¸¸¦ È®º¸.
  • SMART technology°ú RACE ±â¹ýÀÇ Á¶ÇÕÀ¸·Î, Àúºóµµ(low frequency)ÀÇ TCR mutation °ËÃâ °¡´É
  • ÃÖ´ë 8Á¾·ùÀÇ ´Ù¸¥ ¼¼Æ÷ »ùÇà ºÐ¼®
  • single cell TCRºÐ¼®°ú µ¿½Ã¿¡ 5'DE¹ßÇö Çؼ®ÀÌ °¡´É(coming soon)

T¼¼Æ÷°¡ °®´Â Ç׿øƯÀ̼º¿¡´Â ƯÁ¤ TCR ¥á-chain, ¥â-chainÀÇ pairingÀÌ °ü·ÃµÇ±â ¶§¹®¿¡ pairingÁ¤º¸¸¦ ¾ò´Â °ÍÀº Ç׿øÀνĿ¡ °üÇÑ ¿¬±¸¿¡ À־ ¸Å¿ì Áß¿äÇÕ´Ï´Ù. ±âÁ¸¿¡´Â ´ÜÀÏ T¼¼Æ÷ÀÇ ½ÃÄö½º´Â, °³°³ÀÇ T¼¼Æ÷¿¡ À־ÀÇ¥á-chain, ¥â-chainÀÇ pairingÀ» °áÁ¤ÇÒ ¼ö ÀÖ½À´Ï´Ù¸¸, ´Ù¼öÀÇ ¼¼Æ÷¸¦ °³º°ÀûÀ¸·Î sequencingÇÒ ÇÊ¿ä°¡ Àֱ⠶§¹®¿¡, ¸Å¿ì ºñ¿ëÀÌ ³ô°í º¹ÀâÇÑ ºÐ¼®ÀÌ ÇÊ¿äÇß½À´Ï´Ù.
ICELL8 cx TCR Chip°ú °ü·Ã ½Ã¾àÀ» ÀÌ¿ëÇÏ¿© ICELL8 cx½Ã½ºÅÛ¿¡¼­ ´ÜÀϼ¼Æ÷ ¼ö¹é °³ÀÇ T¼¼Æ÷ ¥á chain°ú ¥â chainÀÇ Á¤º¸¸¦ 1°³ÀÇ ½ÃÄö½º¿ë library·Î Çؼ®ÇÒ ¼ö ÀÖ½À´Ï´Ù. ¶Ç Indexing Primer Set¸¦ ÀÌ¿ëÇÏ¿© ÃÖ´ë 10ÀåÀÇ ICELL8 TCR Chip¿¡¼­ À¯·¡ÇÑ cDNA¶óÀ̺귯¸®¸¦ poolingÇÏ¿©, µ¿½Ã¿¡ NGSºÐ¼®ÇÒ ¼ö ÀÖ½À´Ï´Ù. º» Á¦Ç°Àº SMART technology¿¡ ÀÇÇÑ cDNAÇÕ¼º°ú RACE ±â¹ýÀÇ TCRÀ¯ÀüÀÚ Æ¯ÀÌÀû PCR¸¦ Á¶ÇÕÇÏ¿© TCR ¥á-chain¹× TCR ¥â-chainÀÇ °¡º¯¿µ¿ª(VDJ)ÀÇ full-length¸¦ ÁõÆø½Ãų ¼ö ÀÖ¾î ³ôÀº °¨µµÀÇ TCR sequencingÀÌ °¡´ÉÇÕ´Ï´Ù.

ICELL8 cx TCR Chip(5,184 nano well)¿¡´Â 1,728Á¾·ùÀÇ pool-barcode(SMARTer Oligo¿¡ well-barcode ºÎ°¡)°¡ ¹Ì¸® ÇÁ¸°Æ®µÇ¾î ÀÖÀ¸¸ç, °¢ barcode´Â Ĩ À§¿¡ 3well¾¿ ÇÁ¸°Æ®µÇ¾î ÀÖ½À´Ï´Ù. ICELL8 cx ½Ã½ºÅÛÀ» ÀÌ¿ëÇØ T¼¼Æ÷¸¦ chip¿¡ ºÐÁÖÈÄ, ½Ã½ºÅÛ »ó¿¡¼­ imagingÇÏ¿© »ì¾ÆÀÖ´Â ´ÜÀÏ T¼¼Æ÷¸¦ Æ÷ÇÔÇÑ wellÀ» ¼±ÅÃÇÕ´Ï´Ù. RT-PCR ½Ã¾àÀ» ¼±ÅÃÇÑ »ì¾Æ ÀÖ´Â ´ÜÀÏ T¼¼Æ÷¸¦ Æ÷ÇÔÇÑ well¿¡¸¸ ºÐÁÖÇÏ¿©, cDNA ÇÕ¼º°ú ÁõÆø¹ÝÀÀÀ» °¢ well¿¡¼­ ½Ç½ÃÇÕ´Ï´Ù. ´ÙÀ½¿¡ barcodeºÎ¿©µÈ cDNAÁõÆø »ê¹°À» chip¿¡¼­ ȸ¼ö, poolingÇÏ¿©, Á¤Á¦ÇÑ cDNAÀ» 1st TCR-specific PCRÀÇ template·Î »ç¿ëÇÕ´Ï´Ù. À̾î 2nd TCR-specific PCR¿¡ ÀÇÇÑ Illumina index primer°¡ ºÎ¿©µÇ¾î Illumina sequencer¿¡¼­ NGSºÐ¼®ÀÌ °¡´ÉÇÑ cDNA library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ½À´Ï´Ù.

* ´ÜÀϼ¼Æ÷ TCRºÐ¼®°ú µ¿½Ã¿¡ 5'DEÇؼ®ÀÌ °¡´É
º» Á¦Ç°À» ÀÌ¿ëÇÑ ÀÀ¿ë ¿¹·Î¼­ 5'¸»´ÜÀÇ Ä¸Ã³¿¡ ÀÇÇÑ À¯ÀüÀÚ Â÷ÀÌ ¹ßÇö Çؼ®À» Çѹø¿¡ ¼¼Æ÷ÀÇ TCRºÐ¼®°ú º´ÇàÇÒ ¼ö ÀÖ½À´Ï´Ù. ȸ¼öÇÑ cDNAÁõÆø »ê¹°ÀÇ ÀϺθ¦ ÀÌ¿ëÇÏ¿© 5'DEÇؼ®¿ë library¸¦ Á¦ÀÛÇÔÀ¸·Î½á ƯÁ¤ clonotypeÀ» °¡Áø T¼¼Æ÷¿¡ ´ëÇÑ À¯ÀüÀÚ ¹ßÇö Çؼ®ÀÌ °¡´ÉÇÕ´Ï´Ù.

Ç¥ 1. ICELL8 TCR Chip¿Í ½Ã¾àÀÇ ÇÊ¿ä·®
Code

Á¦Ç°¸í

¿ë·®

ÇÊ¿ä·®

640200

ICELL8 cx TCR Chip

1 Chip

1

640179

ICELL8 Human TCR a/b Profiling - Indexing Primer Set

1 Set

1

640182

ICELL8 Human TCR a/b Profiling Reagent Kit

1 Kit

1

640048

ICELL8 Collection Kit

1 Kit

1

640197

ICELL8 cx Loading Kit

1 Kit

1


1ÀåÀÇ ICELL8 cx TCR ChipÀ» »ç¿ëÇÏ´Â °æ¿ì´Â »ó±â Á¦Ç° ¸®½ºÆ® Á¦Ç°À» °¢ 1Á¡¾¿ ±¸ÀÔÇÏ¿©¾ß ÇÕ´Ï´Ù. º°¸ÅÀÇ Indexing Primer SetÁ¦Ç°À» ±¸¸ÅÇÒ °æ¿ì ÃÖ´ë 10ÀåÀÇ ICELL8 cx TCR ChipÀ» polloingÇÏ¿© NGSºÐ¼®ÇÒ ¼ö ÀÖ½À´Ï´Ù.

MSND 384-well Source Plate and Seals´Â ¼¼Æ÷ ÇöŹ¾×(Cell suspension)ÀÇ ºÐÁÖ¿¡ ÀÌ¿ëÇÏ´Â source plate¿Í sealÀÇ ¼¼Æ®ÀÔ´Ï´Ù. Ç¥ 1ÀÇ ÇÊ¿ä ½Ã¾à ¿Ü¿¡ ±¸ÀÔÀÌ ÇÊ¿äÇÕ´Ï´Ù.

Cell suspension Áغñ Á¶°Ç°ËÅä¿Í ºÐÁÖÅ×½ºÆ®¸¦ ÇÒ ¶§¿¡´Â °¢ well¿¡ preprinted oligo(dT) primer°¡ ¹ÌÆ÷ÇÔµÈ Blank ChipÀ» ÀÌ¿ëÇϽʽÿÀ.
³»¿ë
ICELL8 Human TCR a/b Profiling - Indexing Primer Set£¨Code 640179£©¡¡for 1 Chip
       ICELL8 TCRa Human Primer 2 Forward HT Index 1 (aF1£»2 ¥ìM)
       ICELL8 TCRb Human Primer 2 Forward HT Index 1 (bF1£»2 ¥ìM)
ICELL8 Human TCR a/b Profiling - Indexing Primer Set£¨ Code 640180£©¡¡for 5 Chip
       ICELL8 TCRa Human Primer 2 Forward HT Index 1-5 (aF1-aF5£»2 ¥ìM)
       ICELL8 TCRb Human Primer 2 Forward HT Index 1-5 (bF1-bF5£»2 ¥ìM)
ICELL8 Human TCR a/b Profiling - Indexing Primer Set£¨ Code 640181£©¡¡for 10 Chip
       ICELL8 TCRa Human Primer 2 Forward HT Index 1-10 (aF1-aF10£»2 ¥ìM)
       ICELL8 TCRb Human Primer 2 Forward HT Index 1-10 (bF1-bF10£»2 ¥ìM)
ICELL8 Human TCR a/b Profiling Reagent Kit£¨ Code 640182£©
       Package 1£º Control Jurkat Total RNA (1 ¥ìg/¥ìl)
       Package 2£º Second Diluent (100X)
               RNase Inhibitor (40 U/¥ìl)
               ICELL8 Fiducial Mix (1X)
               cDNA Amplification dNTP Mixture (25 mM each)
               Triton X-100 (3£¥)
               SMARTScribe Buffer (10X)
               GC Melt (5 M)
               MgCl2 (1 M)
               Oligo dT Amp Primer (100 ¥ìM)
               DTT (100 mM)
               SMARTScribe Reverse Transcriptase (100 U/¥ìl)
               cDNA Amplification Buffer (2X)
               ICELL8 Amp Primer (10 ¥ìM)
               TCRa Human Primer 1 (2 ¥ìM)
               TCRb Human Primer 1 (2 ¥ìM)
               cDNA Amplification Polymerase (50X)
               P5 Primer (5 ¥ìM)
               TCR Amplification Polymerase (1.25 U/¥ìl)
               TCR Amplification Buffer (5X)
               TCR Amplification dNTP Mixture (2.5 mM each)
               Nuclease-Free Water

ICELL8 Collection Kit (Code 640048)
       Collection Fixture
       Collection Tube
       Collection Film
ICELL8 cx Loading Kit (Code 640197)
       Optical Imaging Film
       Sealing Film
       Blotting Paper
       SmartChip Intermediate Film
MSND 384-well Source Plate and Seals (Code 640018)
      ¤ý2 packs 384-Well Source Plate Seals (10 per pack)
      ¤ý1 pack 384-Well Source Plate (20 per pack)

º¸Á¸
Control Jurkat Total RNA£º£­70¡É
ICELL8 Collection Kit£º½Ç¿Â
ICELL8 Loading Kit B£º½Ç¿Â
±âŸ£º£­20¡É



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