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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > NGS ºÐ¼®¿ë È¿¼Ò > PrimeSTAR¢ç LongSeq DNA Polymerase
50 kb ÁõÆø - high fidelity PCR È¿¼Ò

PrimeSTAR¢ç LongSeq DNA Polymerase

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
R055A
PrimeSTAR¢ç LongSeq DNA Polymerase
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
200 ȸ
354,400¿ø 
443,000¿ø
°¡°ÝÇÒÀÎ
09.01 ~ 10.31
R055S_R055A_DS.v2503Da.pdf
Takara
R055B
PrimeSTAR¢ç LongSeq DNA Polymerase
°ü·ÃÇмú ±¸¸ÅÇϱâ
800 ȸ (R055Ax4)
1,594,800¿ø 

  • ´ÙÄ«¶óÀÇ µ¶ÀÚ ±â¼ú·Î long fragment (50 kb ÀÌ»ó) ÁõÆø ½ÇÇö
  • GC/AT rich ¼­¿­ÀÇ long fragmentµµ ÁõÆø
  • ³ôÀº ƯÀ̼º: ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¾ïÁ¦Çϰí long fragmentÀÇ multiplex PCR Àû¿ë °¡´É
  • ³ôÀº Á¤È®µµ·Î Long read sequencing¿¡ ÃÖÀû
  • »ç¿ëÀÌ Æí¸®ÇÑ premix ½Ã¾à
Á¦Ç°¼³¸í
PrimeSTAR¢ç LongSeq DNA Polymerase´Â ¼¼°è ÃÖ°í ¼öÁØÀÇ ³ôÀº ƯÀ̼ºÀ» °¡Áø PCR È¿¼ÒÀÌ´Ù. ³ôÀº ƯÀ̼ºÀ» °¡Á® ºñƯÀÌÀû ÁõÆøÀ» ¾ïÁ¦Çϸ鼭 50kb ÀÌ»óÀÇ Å¸°Ù ÁõÆøÀÌ °¡´ÉÇϰí, GC/AT rich ÇÑ long fragment µî ÁõÆøÀÌ ¾î·Á¿î ¼­¿­µµ ¾çÈ£ÇÏ°Ô ÁõÆøÇÒ ¼ö ÀÖ´Ù. ÀÌ·¯ÇÑ Æ¯Â¡À» ÅëÇØ ±âÁ¸¿¡ ¾î·Á¿ü´ø long fragmentÀÇ multiplex PCR ¹ÝÀÀÀ» ¼öÇàÇÒ ¼ö ÀÖ°í, º¸´Ù ³ôÀº Á¤È®¼ºµµ °âºñÇϱ⠶§¹®¿¡ long read sequencingÀ» À§ÇÑ ÁÖÇü ÁõÆø¿¡ ÃÖÀûÀÌ´Ù.
º» Á¦Ç°Àº ¸ðµç ±¸¼º½Ã¾à (È¿¼Ò, buffer, dNTP Mixture µî)ÀÌ Æ÷ÇԵǾî ÀÖ´Â 2¡¿ premix ŸÀÔÀ̸ç, -20¡É¿¡¼­ ¾óÁö ¾Ê¾Æ, Áï½Ã primer¿Í templateÀ» Ãß°¡ÇÏ¿© ÀçÇö¼º ³ôÀº PCRÀ» ÁøÇàÇÒ ¼ö ÀÖ´Ù.
¶ÇÇÑ, º» È¿¼Ò´Â »ó¿Â¿¡¼­ DNA Polymerase Ȱ¼º ¹× 3¡¯ ¡æ 5¡¯ exonuclease Ȱ¼ºÀ» ¾ïÁ¦ÇÏ´Â ´ÜÀÏ Å¬·Ð Ç×ü¸¦ ÀÌ¿ëÇÑ hot start versionÀÇ PCR È¿¼ÒÀÌ´Ù.
³»¿ë (200 ȸ, 50 §¡ ¹ÝÀÀ½Ã)
PrimeSTAR¢ç LongSeq DNA Polymerase (Code R055A)
1. PrimeSTAR LongSeq Premix (2¡¿)

1 §¢ x 5

º¸Á¸
-20¡É
½ÇÇè ¿¹
(1) Ÿ°Ù »çÀÌÁî
PrimeSTAR¢ç LongSeq DNA Polymerase (Code R055A/B)´Â ¶Ù¾î³­ ½ÅÀ强À» °¡Áö¸ç, ÀϹÝÀûÀ¸·Î High Fidelity PCR È¿¼Ò¿¡¼­´Â ÁõÆøÇϱ⠾î·Á¿î long fragment (53 kb) ±îÁö ƯÀ̼º ³ô°Ô ÁõÆø °¡´ÉÇÏ´Ù.

1. p53

0.5kb

5. ¥â-globin

15kb

9. SLC30A9

53kb

2. DCLRE1A

1kb

6. ¥â-globin

24kb

M1. 1kb DNA Ladder

3. DCLRE1A

2kb

7. ¥â-globin

30kb

 

 

4. DCLRE1A

4kb

8. HBB

40kb

 

 



Clontech PCR Thermal Cycler GP (Code WN400) »ç¿ë

(2) GC/AT rich Ÿ°ÙÀÇ ÁõÆø
ºñƯÀÌÀûÀÎ ÁõÆøÀÌ ÀϾ±â ½¬¿î GC/AT rich ÀÎ ÁÖÇüµµ, PrimeSTAR¢ç LongSeq DNA Polymerase (Code R055A/B)¿¡¼­´Â Ưº°ÇÑ buffer³ª ¹ÝÀÀ Á¶°ÇÀ» ¼³Á¤ÇÏÁö ¾Ê°í long fragment ÁõÆøÀÌ °¡´ÉÇÏ´Ù.
- »ùÇÃ: human genomic DNA (100 ng/50 §¡ ¹ÝÀÀ°è)


Clontech PCR Thermal Cycler GP (Code WN400) »ç¿ë
°ü·ÃÁ¦Ç°
- A-tailing & TA cloning kit: Mighty TA-cloning Reagent Set for PrimeSTAR¢ç
- Á¤È®ÇÑ cDNA ÇÕ¼ºÀ» À§ÇÑ RT premix: PrimeScript¢â IV 1st strand cDNA Synthesis Mix
- Gel elution¿ë Agarose: SeaPlaque¢ç Agarose
- 5ºÐ¸¸¿¡ Àü±â¿µµ¿ ¿Ï·á! Precast gel system: FlashGel¢â System
- ÄÄÆÑÆ®ÇÑ 96 well gradient PCR ±â±â: Clontech PCR Thermal Cycler GP

Keyword :

NGS ºÐ¼®¿ë È¿¼Ò
>
50 kb ÁõÆø - high fidelity PCR È¿¼Ò
PrimeSTAR¢ç LongSeq DNA Polymerase
>
Single-stranded DNA °£ÀÇ ligation
Single strand DNA Ligase