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> pCold¢â TF DNA
Soluble tag (TF tag) ÀÌ Æ÷ÇÔµÈ Cold shock Vector
pCold¢â TF DNA
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Takara
3365
pCold¢â TF DNA
25 ¥ìg
1,011,200¿ø
1,264,000¿ø
09.02 ~ 10.31
Product index
Overview
´Ü¹éÁú ¹ßÇö½Ã½ºÅÛ - »ó..
[°³¿ä] Â÷¼¼´ë ´Ü¹éÁú ..
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
[¼±Åð¡À̵å] Protein E..
His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦
[¼±Åð¡À̵å] His-tag ..
Capturem¢â His-Tagged ..
TALON (Cobalt) resin ..
TALON Magnetic Beads
TALON (Cobalt) Superfl..
xTractor Buffer Kit
His60 (Nickel) Superfl..
His60 Ni Magnetic Bead..
HRV 3C Protease
Poly Histidine tag Ç×..
TALON (Cobalt) CellThr..
TALONSpin Column
´Ü¹éÁú ÃßÃâ ¡¤ Á¤Á¦°ü·Ã
Calpastatin
Cryonase¢â Cold-active..
Glutathione Excellose ..
IDA Excellose (polyhis..
MBP Excellose (MBP fus..
MiniExcellose
ProteoGuard EDTA-Free ..
Streptavidin-based Enr..
Ç×ü Á¤Á¦
Ç×ü Á¤Á¦ Resin
Capturem¢â IP £¦ Co-IP..
°ïÃæ ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö (Bac..
[Overview] Insect Expr..
BacPAK¢â Baculovirus G..
BacPAK¢â p35 ELISA Kit..
BacPAK Baculovirus ¹ß..
Baculovirus control vi..
Baculovirus DNA packag..
Baculovirus Titer Kit
flashBAC¢â Baculovirus..
FoldHelper Helper viru..
ProEasy Baculovirus Ve..
ProFectin
ProFold Baculovirus Ve..
qPCR Titer ÃøÁ¤ Kit
Transfer Vectors
Æ÷À¯·ù ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö
[Overview] Mammalian E..
CHOgro¢ç Expression Sy..
CHOgro¢ç High Yield Ex..
Corystein¢â£¨Purothion..
Human Cell-Free Protei..
pHEK293 Ultra Expressi..
´ëÀå±Õ ´Ü¹éÁú ¹ßÇö
[Overview] Bacterial E..
´ëÀå±Õ ´Ü¹éÁú ¹ßÇö £¦ ..
Anti-ProS2
Anti-TF(Trigger Factor..
Chaperone Competent Ce..
Chaperone Plasmid Set
HAT ´Ü¹éÁú ¹ßÇö£¦ Á¤Á¦..
pCold¢â GST DNA
pCold¢â ProS2 DNA
pCold¢â TF DNA
pCold¢â Vector ½Ã¸®Áî
Single Protein Product..
TaKaRa Competent Cell ..
Brevibacillus£¯Bacillus ¹ß..
B. subtilis Secretory ..
´Ù¾çÇÑ tag ´Ü¹éÁú °ü·Ã
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
Excellose Spin Kit His..
FLAG tag ´Ü¹éÁú »ý»ê
GST-tag À¶ÇÕ ´Ü¹éÁú Á¤..
´ç£¯ÀλêÈ ´Ü¹éÁú °ü·Ã
Glycoprotein ³óÃà¿ë Re..
Magnetic bead - Phosph..
Phosphoprotein enrichm..
´Ü¹éÁú ¿°»ö ½Ã¾à
TaKaRa CBB Protein Saf..
SYPRO Protein Gel Stai..
SYPRO Ruby Protein Sta..
´Ü¹éÁú °ËÃâ, Á¤·®, Çؼ®
HRP Substrate ½Ã¸®Áî
HRP-Juice
ProSieve¢â EX Running ..
TaKaRa BCA Protein Ass..
TaKaRa Bradford Protei..
Tris-Glycine Powder
Western BLoT Blocking/..
Western BLoT Immuno Bo..
Western BLoT Immuno Bo..
Western BLoT Rapid Det..
Protease (¾Æ¹Ì³ë»ê ¼¿ ÇØ..
Arginylendopeptidase (..
Pfu Aminopeptidase I
Pfu Methionine Aminope..
Pfu N-acetyl Deblockin..
Pfu Protease S
Pfu Pyroglutamate Amin..
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pCold¢â TF DNA´Â
E. coli
ChaperoneÀÇ ÇϳªÀÎ Trigger Factor (TF, ºÐÀÚ·® 48kDa)¸¦ soluble tag·Î ÇÏ´Â fusion Cold Shock expression vectorÀÌ´Ù. pCold DNA¿Í ¸¶Âù°¡Áö·Î
E. coli
Cold Shock À¯ÀüÀÚÀÇ ÇϳªÀÎ
cspA
À¯ÀüÀÚÀÇ promoter¸¦ ÀÌ¿ëÇÏ°í ÀÖÀ¸¸ç,
cspA
promoterÀÇ down-stream¿¡ 5¡¯ untranslated region (5'UTR), translation enhancing element (TEE), His tag, TF tag, multi-cloning site (MCS) µîÀÌ ¹èÄ¡µÇ¾î ÀÖ°í, Promoter downstream¿¡´Â ¹ßÇöÀ» Á¦¾îÇϱâ À§ÇÑ
lac
operator°¡ »ðÀԵǾî ÀÖ´Ù.
TF tag¿Í MCS »çÀÌ¿¡´Â HRV 3C Protease, Thrombin, Factor Xa Àνļ¿ÀÌ ÀÖ¾î ¹ßÇö ÈÄ À¶ÇÕ ´Ü¹éÁú¿¡¼ tag¸¦ Á¦°ÅÇÏ´Â µ¥¿¡ »ç¿ëÇÒ ¼ö ÀÖ´Ù.
Cold Shock expression systemÀº Àú¿Â¿¡¼ ¸ñÀû´Ü¹éÁúÀÇ ¹ßÇöÀ» À¯µµÇϱ⠶§¹®¿¡ ¼÷ÁÖ À¯·¡ ´Ü¹éÁúÀÇ ÇÕ¼ºÀº ¾ïÁ¦µÇ°í ¸ñÀû ´Ü¹éÁú¸¸À» °íÈ¿À²·Î ¾òÀ» ¼ö ÀÖ¾î ±âÁ¸ÀÇ
E. coli
¹ßÇö½Ã½ºÅÛ°ú ºñ±³ÇÏ¿© ¸ñÀû ´Ü¹éÁúÀÇ ¹ßÇö·®À̳ª °¡¿ë¼ºÀÇ Çâ»óÀ» ±â´ëÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ TF soluble tag°ú chaperone ±â´ÉÀ¸·Î ÀÎÇØ ±× µ¿¾È ¹ßÇöÀÌ ¾î·Á¿ü´ø À¯ÀüÀÚ¸¦ º¸´Ù ³ôÀº È®·ü·Î soluble proteinÀ¸·Î ¹ßÇö½Ãų ¼ö ÀÖ´Ù.
*Translation enhancing elementÀÇ ¾àÀÚ·Î, ¹ø¿ªÀ» ÃËÁøÇÏ´Â ±â´ÉÀ» °¡Áø´Ù.
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pCold¢â TF vector map
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-20 ¡É
GenBank
Accession No. : AB213654
»ç¿ë »ó ÁÖÀÇ»çÇ×
º» Á¦Ç°Àº ¿¬±¸ ¸ñÀûÀ¸·Î¸¸ »ç¿ë °¡´ÉÇÑ Á¦Ç°ÀÔ´Ï´Ù (Research use only).
º» Á¦Ç° ¶Ç´Â º» Á¦Ç°À¸·Î ¸¸µé¾îÁø »ê¹°À» »ó¾÷Àû ¸ñÀûÀ¸·Î ÀÌ¿ëÇÏ´Â °æ¿ì, ¶óÀ̼±½º°¡ Æ÷ÇÔµÈ Á¦Ç°À» ±¸ÀÔÇÏ½Ç ¼ö ÀÖÀ¸´Ï ´ÙÄ«¶óÄÚ¸®¾Æ (02-2081-2510)·Î º°µµ ¹®Àǹٶø´Ï´Ù.
°ü·ÃÁ¦Ç°
- ÀçÁ¶ÇÕ ´Ü¹éÁú ¹ßÇö¿ë
E. coli
Comp. cell :
TaKaRa Competent Cells BL21
- Â÷¼¼´ë Membrane ±â¼úÀÇ His-tagged protein Á¤Á¦ column :
Capturem¢â His-Tagged Purification
- Acetic acid ¾ø´Â °í°¨µµ ´Ü¹éÁú ¿°»ö½Ã¾à :
TaKaRa CBB Protein Safe Stain
- ¹ü¿ëÀûÀÎ °ËÃâºÎÅÍ ÃÊ°í°¨µµ °ËÃâ±îÁö ´Ù¾çÇÑ Á¦Ç° ¶óÀξ÷ :
HRP Substrate ½Ã¸®Áî
Keyword :
´ëÀå±Õ ´Ü¹éÁú ¹ßÇö
[Overview] Bacterial Expression System
´ëÀå±Õ ´Ü¹éÁú ¹ßÇö £¦ Á¤Á¦ Starter Kit
Anti-ProS2
Anti-TF(Trigger Factor)
ChaperoneÀ» ¹ßÇöÇÏ´Â E. coli BL21 Competent ..
Chaperone Competent Cell BL21 ½Ã¸®Áî
ÀûÀýÇÑ foldingÀ» À§ÇÑ chaperone team
Chaperone Plasmid Set
HAT ´Ü¹éÁú ¹ßÇö£¦ Á¤Á¦ ½Ã½ºÅÛ
GST TagÀÌ Æ÷ÇÔµÈ Cold shock Vector
pCold¢â GST DNA
Soluble tagÀÇ °áÁ¤ÆÇ
pCold¢â ProS2 DNA
Soluble tag (TF tag) ÀÌ Æ÷ÇÔµÈ Cold shock Ve..
pCold¢â TF DNA
°íÈ¿À² E. coli Cold shock ¹ßÇö½Ã½ºÅÛ
pCold¢â Vector ½Ã¸®Áî
¸ñÀû ´Ü¹éÁú Àüü¸¦ È¿À²ÀûÀ¸·Î À¯µµ¹ßÇö/µ¿À§..
Single Protein Production System (SPP System¢â)
TaKaRa Competent Cell BL21