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Magnetic bead - Phosphoprotein enrichment kit

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Clontech
635641
TALON PMAC Magnetic Phospho Enrichment Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 Kit
973,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33128, x33129

phosphoproteinÀ» microscale·Î Á¤Á¦Çϱâ À§ÇÑ ¿Ïº®ÇÑ kit
  • ¼¼Æ÷³ª Á¶Á÷ ½Ã·á¿¡ °ü°è¾øÀÌ Magnetic Bead¸¦ ÀÌ¿ëÇÏ¿© ºü¸£°í ½±°Ô phospho proteinÀ» ³óÃà
  • Final elute: ¼¼Æ÷ Á¾·ù¿¡ µû¸¥ 14-17% phosphoprotein Á¤Á¦
  • ¼¼Æ÷ ½ÅÈ£ ¿¬±¸ (ºñ¹æ»ç¼±)³ª MS ÀÀ¿ë¿¡ ÀûÇÕ
Magnetic Phospho Enrichment Kit´Â Clontech TALON ±â¹ÝÀÇ Phosphop rotein Enrichment KitÀÇ Àλê ƯÀ̼º°ú ºÐ¸®°¡ ¿ëÀÌÇÑ magnetic bead¸¦ °áÇÕÇÏ¿© mammalian ¼¼Æ÷¿Í Á¶Á÷¿¡¼­ ÀλêÈ­µÈ ´Ü¹éÁúÀ» microgram ´ÜÀ§·Î ºÐ¸®Çϱâ À§ÇÑ °£´ÜÇÏ°í, ºü¸¥ metal affinity ±â¹ÝÀÇ ¹æ¹ýÀ» Á¦°øÇÑ´Ù (1). bead ¾ÈÀÇ ÀÚ¼º ÀÔÀÚ´Â ÀÚ¼º ºÐ¸®±â¸¦ ÀÌ¿ëÇÏ¿© ±Ø¼Ò·®ÀÇ phosphoproteinÀ» ºü¸£°í ½±°Ô Á¤Á¦ÇÒ ¼ö ÀÖ°Ô ÇÑ´Ù.
ÀûÀº ºÎÇÇÀÇ ÃßÃâ¾×¿¡¼­ÀÇ ±Ø¼Ò·®ÀÇ Á¤Á¦
Phospho Magnetic Bead´Â 5% ÇöŹ¾×À¸·Î Á¦°øµÇ¸ç ÇöŹ¾× ml ´ç 400 ¥ìgÀÇ ¥â-casein °áÇÕ·ÂÀ» ³ªÅ¸³½´Ù. PhosphoproteinÀº ³óÃàµÈ ½Ã·á·Î ¾ò±â À§ÇÏ¿© ÀûÀº ºÎÇÇ (50-200 ¥ìl)·Î elutionÇÒ ¼ö ÀÖ´Ù.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
References
1. TALON PMAC MagneticPhospho Enrichment Kit (July 2006) Clontechniques XXI(2):9.

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