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HRV 3C Protease

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Takara
7360
HRV 3C Protease
°ü·ÃÇмú ±¸¸ÅÇϱâ
500 U
318,400¿ø 
398,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
7360_e.v1612Da.pdf

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  • 4¡É¿¡¼­ ´Ü¹éÁú ºÐÇØ È¿¼Ò È°¼ºÀ» ³ªÅ¸³»±â ¶§¹®¿¡ ¸ñÀû ´Ü¹éÁúÀ» Àú¿ÂÀ¸·Î À¯ÁöÇϸ鼭 tag¸¦ Àý´Ü °¡´É
  • Protease ÀÚü°¡ 6¡¿HN tag¸¦ º¸À¯ÇÏ°í ÀÖ¾î Àý´Ü ÈÄ TALON resin µîÀ¸·Î Á¦°Å °¡´É
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HRV 3C Protease´Â Human Rhinovirus 14 type 3C Protease¸¦ ´ëÀå±Õ¿¡¼­ ¹ßÇö½ÃŲ °í¼øµµ·Î Á¤Á¦µÈ 6¡¿HN À¶ÇÕ ´Ü¹éÁúÀÌ´Ù. º» È¿¼Ò´Â Native È¿¼Ò¿Í µ¿µîÇÑ È°¼ºÀ» °®°í ÀÖ°í ƯÁ¤ ¾Æ¹Ì³ë»ê ¼­¿­ÀÎ LeuGluValLeuPheGln¡éGlyPro¸¦ ÀνÄÇÏ¿© Àý´ÜÇÑ´Ù. ´Ü¹éÁúÀÇ ÅÂ±× ¹è¿­ÀÇ Àý´Ü¿¡ ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù. º» È¿¼Ò´Â 6¡¿HN À¶ÇÕ ´Ü¹éÁú·Î ¹ÝÀÀ ÈÄ, TALON Metal Affinity ResinÀ̳ª His60 Ni Superflow Resin°ú °°Àº IMAC(immobilized metal affinity chromatography)¿¡ ÈíÂøÇϱ⠶§¹®¿¡ ¹ÝÀÀ¾×¿¡¼­ ½±°Ô Á¦°ÅÇÒ ¼ö ÀÖ´Ù. ¶ÇÇÑ ÀÌ È¿¼Ò´Â 4¡É¿¡¼­ ÃæºÐÇÑ È°¼ºÀ» ³ªÅ¸³»±â ¶§¹®¿¡ ¸ñÀû ´Ü¹éÁúÀÇ È°¼ºÀ» ÀúÇØÇÏÁö ¾Ê°í ¾ÈÁ¤ÀûÀÎ »óÅ¿¡¼­ Àý´ÜÀ» ÇÒ ¼ö ÀÖ´Ù.
º» Á¦Ç°¿¡´Â ¹ÝÀÀ ÄÁÆ®·Ñ·Î Cleavage Control Fusion ProteinÀÌ Æ÷ÇԵǾî ÀÖ´Ù. Control ProteinÀº N¸»´Ü¿¡ 6¡¿HN ű׸¦ °®°í ÀÖÀ¸¸ç HRV 3C Protease¿¡ ÀÇÇØ 52 kDaÀÇ Trigger Factor¿Í 24 kDaÀÇ GST¿¡ Àý´ÜµÇ±â ¶§¹®¿¡ Àý´Ü ÈÄ, SDS-PAGE¿¡¼­ ½±°Ô °á°ú¸¦ È®ÀÎÇÒ ¼ö ÀÖ´Ù.
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HRV 3C Protease£¨1 U/¥ìl£©

500 U

Cleavage Control Fusion Protein£¨1 ¥ìg/¥ìl£©

10 ¥ìg

10¡¿HRV 3C Cleavage Buffer

10 ml

ÀúÀå
- 20¡É
Çü»ó ¡¤ Á¶¼º

HRV 3C Protease Storage Buffer

50 mM

Tris-HCl(pH8.0 at 25¡É)

150 mM

NaCl

1 mM

EDTA

0.5 mM

THP

50%

Glycerol


Cleavage Control Fusion Protein Storage Buffer

50 mM

Tris-HCl(pH7.5 at 25¡É)

100 mM

NaCl

10 mM

EDTA


10¡¿HRV 3C Cleavage Buffer

500 mM

Tris-HCl(pH7.5 at 25¡É)

1.5 M

NaCl

È°¼ºÀÇ Á¤ÀÇ
HRV 3C Cleavage Buffer(50 mM Tris-HCl, 150 mM NaCl, pH7.5)Áß¿¡¼­ 100¥ìgÀÇ Cleavage Control Fusion Protein ±âÁúÀ» 4¡É, 16½Ã°£¿¡ 95% ÀÌ»ó Àý´ÜÇÏ´Â È°¼ºÀ» 1 U·Î ÇÑ´Ù.

Keyword :

His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦
>
Spin columnŸÀÔÀ¸·Î ºü¸£°í °£ÆíÇÑ His-tag ´Ü..
Capturem¢â His-Tagged Purification
>
His-tagged protein °í¼øµµ Á¤Á¦
TALON (Cobalt) resin & column
>
ºü¸£°Ô ¼Ò¿ë·®ÀÇ his-tagged protein Á¤Á¦
TALON Magnetic Beads
>
FPLC purification - His-tagged protein
TALON (Cobalt) Superflow resin & cartridge
>
´ëÀå±ÕÀ¸·ÎºÎÅÍ His-tag À¶ÇÕ ´Ü¹éÁú ÃßÃâ¿¡,xT..
xTractor Buffer Kit
>
°í¼öÀ² Ni resin & FPLC purification
His60 (Nickel) Superflow resin & cartridge
>
High-throughput & microscale
His60 Ni Magnetic Beads
>
4¡É¿¡¼­ È°¼ºÀ» ¶ç´Â HN-tag¸¦ °®°í ÀÖ´Â ´Ü¹é..
HRV 3C Protease
>
Cell lysate¿¡¼­ His-tagged protein Á÷Á¢ Á¤Á¦
TALON (Cobalt) CellThru resin
>
Prepacked spin column
TALONSpin Column