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Biotinylated proteinsÀÇ Á¤Á¦¿Í ³óÃàÀ» À§ÇÑ Membrane spin column

Streptavidin-based Enrichment & Purification

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
635733
Capturem¢â Streptavidin Miniprep Columns
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
20 columns
779,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x100908, x100924
Clontech
635734
Capturem¢â Streptavidin 96-Well Plate
°ü·ÃÇмú ±¸¸ÅÇϱ⠶óÀ̼±½º 
1 plate
1,462,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x100908

  • Simple, Ultra-fast: ½Ç¿Â¿¡¼­ 5 - 15ºÐ ³», Biotinylated protein ³óÃà/Á¤Á¦ ¿Ï·á
  • High concentration: °í³óµµÀÇ ´Ü¹éÁú Á¤Á¦ °¡´É
  • High quality: ªÀº Á¤Á¦ ½Ã°£À¸·Î ´Ü¹éÁú ÀÀÁý, º¹ÇÕü Çظ® (Complex dissociation), ½ÇÈ° À§ÇèÀ» ÃÖ¼ÒÈ­
  • High reproducibility: well°£ ÀÏÁ¤ÇÑ Á¤Á¦ °á°ú
  • Single use: Clontech µ¶ÀÚÀûÀÎ disposable spin column »ç¿ëÀ¸·Î Á¤Á¦/³óÃà °úÁ¤ ÁßÀÇ ¿À¿° ¹× carryover ¹æÁö

Capturem¢â Streptavidin Á¦Ç°Àº high-capacity Capturem membrane¿¡ streptavidinÀ» °íÁ¤½ÃŲ Á¦Ç°À¸·Î½á, °íÇ°ÁúÀÇ biotinylated proteinÀ» º¸´Ù ºü¸£°í ½±°Ô ³óÃà (enrichment) ¶Ç´Â Á¤Á¦ (purification)ÇÑ´Ù. Capturem¢â Streptavidin membrane¿¡ °áÇÕÇÑ´Ù°í ¾Ë·ÁÁø biotinylated ligand´Â ¸ñÀû ´Ü¹éÁúÀ̳ª complex¸¦ ƯÀÌÀûÀ¸·Î ĸÃÄÇϰųª pull downÇÏ´Â µ¥ »ç¿ëÇÒ ¼ö ÀÖ´Ù. StreptavidinÀº¹ÚÅ׸®¾Æ (Streptomyces avidinii)ÀÇ ¼¼Æ÷º® ´Ü¹éÁú·Î½á biotin¿¡ ƯÀÌÀûÀΠģȭ¼º (high affinity)¸¦ º¸À¯ÇÏ°í ÀÖ´Ù.
Capturem¢â Kit´Â ClontechÀÌ µ¶ÀÚÀûÀ¸·Î Á¦ÀÛÇÑ high-capacity membrane-based affinity technology·Î½á, ½Ç¿Â¿¡¼­ ºü¸¥ ½Ã°£ ³»¿¡ ´Ü¹éÁú ¶Ç´Â Ç×ü µîÀ» °í¼øµµ·Î Á¤Á¦ÇÒ ¼ö ÀÖ´Â ±â¼úÀÌ´Ù.

Ç¥ 1. Loading capacities and times for available Capturem¢â Streptavidin formats
 

Biotin-antibody

Biotin-BSA

Biotin-ssDNA

Free biotin

Purification time

Capturem¢â Streptavidin miniprep columns and 96-well plates

20-40 ¥ìg

>15 ¥ìg

1,000-1,500 ng

>4,000 pmol

15 minutes




±×¸² 1. Successive capture of antibodies in triplicate using Capturem Streptavidin. Panels A-C. First, 48 ¥ìg of biotinylated rabbit IgG in 200 ¥ìl Binding Buffer was passed through an equilibrated Capturem Streptavidin spin column, and 32.0 ¡¾ 1.4 ¥ìg (Panel B) was successfully immobilized on the membrane. Following a single wash step, a sample containing the spiked-in target antibody (~100 ¥ìg of anti-rabbit IgG from goat) in hybridoma medium with 20% mouse serum was diluted with Binding Buffer and applied to the Capturem Streptavidin column. After two successive washing steps with Binding Buffer and then PBS, the enriched target antibody was eluted from the biotinylated capture antibody in 1.0 M glycine in three steps to yield 42 ¡¾ 5 ¥ìg (Panel C) of highly pure target antibody.


±×¸² 2. Highly reproducible capture in Capturem Streptavidin 96-well plates. Eight technical replicates were loaded with either biotinylated oligonucleotide (Panel A) or biotinylated BSA (Panel B) and captured using centrifugation. Bound molecules were quantified by measuring the O.D. of samples before and after loading through the column. Standard protocols with these 96-well plates can be performed in under 15 minutes.
Àû¿ë
  • Purification or enrichment of biotinylated proteins
  • Pull-down of binding partners (e.g., proteins or oligos) for a biotin-labeled ligand
º¸Á¸
½Ç¿Â
±¸¼ºÇ°

Capturem¢â Streptavidin Miniprep Columns (Code 635733)

   - Capturem Streptavidin Miniprep Columns

20 columns

Capturem¢â Streptavidin 96-Well Plate (Code 635734)

   - Capturem Streptavidin 96-Well Plate

1Àå


Keyword : streptavidin,biotin,ºñ¿Àƾ,¹ÙÀÌ¿Àƾ,´Ü¹éÁú,´Ü¹éÁú Á¤Á¦,Resin,Biotinylated protein,Á¤Á¦,avidin,¾Æºñµò

´Ü¹éÁú ÃßÃâ ¡¤ Á¤Á¦°ü·Ã
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Calpastatin
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Biotinylated proteinsÀÇ Á¤Á¦¿Í ³óÃàÀ» À§ÇÑ M..
Streptavidin-based Enrichment & Purification