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BCA ´Ü¹éÁú Á¤·® Kit

TaKaRa BCA Protein Assay Kit

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
T9300A
TaKaRa BCA Protein Assay Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
500ȸ
145,600¿ø 
182,000¿ø
°¡°ÝÇÒÀÎ
09.02 ~ 10.31
T9300A_e.v1906Da.pdf



  • Bicinchoninic acid (BCA) ½Ã¾àÀ» ÀÌ¿ëÇÑ ´Ü¹éÁú Á¤·® kit
  • °£´ÜÇÑ Á¶ÀÛÀ¸·Î ´Ü½Ã°£¿¡ ´Ü¹éÁú Á¤·® °¡´É
  • ´Ü¹éÁú Á¾·ù¿¡ ¿µÇâÀ» ¹ÞÁö ¾Ê¾Æ 0.02~2 mg/§¢ÀÇ ³ÐÀº ¹üÀ§¿¡¼­ ´Ü¹éÁú Á¤·® °¡´É
  • °è¸é È°¼ºÁ¦¿¡ ÀÇÇÑ ¿µÇâÀ» ¹ÞÁö ¾ÊÀ½
Á¦Ç° ¼³¸í
TaKaRa BCA Protein Assay KitÀº ¸Å¿ì ³ôÀº °¨µµÀÇ ¹ß»ö ¹ÝÀÀ(colormetric)À» ÀÌ¿ëÇÏ¿© ´Ü¹éÁúÀÇ ¾çÀ» Á¤·®Çϸç, °è¸éÈ°¼ºÁ¦·Î °¡¿ëÈ­µÈ ´Ü¹éÁúÀÇ Á¤·®µµ °¡´ÉÇÏ´Ù.
BCA ´Ü¹éÁú Á¤·®ÀÇ ¿ø¸®´Â 2´Ü°è·Î ÀÌ·ç¾îÁø´Ù. ù ¹ø° ´Ü°è´Â ¾ËÄ®¸®¼º(¾à ¿°±â¼º)¿ë¾×³»ÀÇ Á¤·®ÇÏ·Á´Â ´Ü¹éÁúÀÇ ÆéŸÀÌµå °áÇÕ¿¡ ÀÇÇØ Cu+2ÀÌ Cu+1À¸·Î ȯ¿øµÈ´Ù(biuret reacton). ÀÌ ¶§, ȯ¿øµÈ Cu+1ÀÇ ¾çÀº ¿ë¾×¿¡ Æ÷ÇÔµÈ ´Ü¹éÁúÀÇ ¾ç¿¡ ºñ·ÊÇÑ´Ù. µÎ ¹ø° ´Ü°è¿¡¼­ 2ºÐÀÚÀÇ Bicinchoninic acid (BCA)°¡ ȯ¿øµÈ Cu+1¿Í ¹èÀ§ °áÇÕÇÏ¿© 562nm¿¡¼­ °­ÇÑ Èí±¤µµ¸¦ ³ªÅ¸³»´Â ÆĶû-º¸¶ó»öÀÇ È­ÇÕ¹°À» Çü¼ºÇÑ´Ù. ÀÌ°ÍÀ» ºÐ±¤ ±¤µµ°è(spectrophotometer)¸¦ »ç¿ëÇÏ¿© ÃøÁ¤ ¹× Á¤·®ÇÑ´Ù.
BCA¿¡ ÀÇÇÑ ´Ü¹éÁú Á¤·®Àº ´Ü¹éÁúÀÇ Á¾·ù¿¡ µû¸¥ Â÷ÀÌ°¡ Àû¾î 0.02~2 mg/§¢ÀÇ ³ÐÀº ¹üÀ§¿¡¼­ Á¤·®ÀÌ °¡´ÉÇϸç, °è¸éÈ°¼ºÁ¦¿¡ ÀÇÇÑ ¿µÇâÀ» ¹ÞÁö ¾Ê´Â´Ù. ÇÏÁö¸¸, ȯ¿øÁ¦ ¹× ų·¹ÀÌÆ®°è ¹°ÁúÀº ¹ÝÀÀ¼º¿¡ ¿µÇâÀ» Áֱ⠶§¹®¿¡, ÇØ´ç ¹°ÁúÀÌ Æ÷ÇÔµÈ ¿ë¾×ÀÇ Á¤·®¿¡´Â ÀûÇÕÇÏÁö ¾Ê´Ù(°øÁ¸¹°ÁúÀÇ ¿µÇâÀº <Ç¥. 1> Âü°í).
´Ü¹éÁúÀÇ Á¤·®Àº º» Á¦Ç°¿¡ Æ÷ÇÔµÈ BSA Ç¥Áع°ÁúÀ» »ç¿ëÇÏ¿© °Ë·® °î¼±(standard curve)À» ÀÛ¼º ÈÄ »ùÇÃÀÇ ³óµµ¸¦ °áÁ¤ÇÑ´Ù.


±×¸² 1. Standard Curve for BSA from 0 - 2,000 ¥ìg/§¢
³»¿ë
1 §¢ ¹ÝÀÀ ½Ã 500ȸ, microtiter plate¸¦ ÀÌ¿ëÇÑ 200 §¡ ¹ÝÀÀ ½Ã 2,500ȸÀÇ ÃøÁ¤À» ÇÒ ¼ö ÀÖ´Ù.
1. BCA Reagent A 250 §¢¡¿2
2. BCA Reagent B 20 §¢
3. BSA Standard Solution (2 mg/ml)* 1 §¢¡¿10
* : BSA Standard SolutionÀº ¾ÈÁ¤È­¸¦ À§ÇØ 0.9% NaCl, 0.05% NaN3 ¸¦ Æ÷ÇÔÇÑ´Ù.
º¸Á¸
BCA reagent A, BCA reagent B : ½Ç¿Â
BSA Standard solution : 4¡É
*º» Á¦Ç°Àº »ó¿Â¿¡¼­ ¹è¼ÛµË´Ï´Ù. Á¦Ç° ¼ö·É ÈÄ BSA Standard SolutionÀº 4¡É¿¡ º¸°üÇϼ¼¿ä.
Á¦Ç° ÀÌ¿Ü¿¡ ÇÊ¿äÇÑ °Í
  • Spectrophotometer and compatible 1 §¢ cuvette
  • Microplate reader and compatible microplate
  • Benchtop centrifuge
  • Microtubes (2 §¢ or 1.5 §¢)
  • Water bath (37¡É)
  • Incubator (60¡É) (for the low-concentration measurement protocol)
´Ü¹éÁú Á¤·® »ùÇÿ¡ °øÁ¸ ¹°Áú¿¡ ÀÇÇÑ ¿µÇâ
BCA ¹æ¹ýÀº ºñ±³Àû °è¸éÈ°¼ºÁ¦³ª Buffer¿¡ ´ëÇÑ ¿µÇâÀ» ¹ÞÁö ¾ÊÁö¸¸, °í³óµµÀÇ Å³·¹ÀÌÆ®°è ¹× ȯ¿øÁ¦ ¹°ÁúÀº ÃøÁ¤¿¡ ¿µÇâÀ» ÁØ´Ù. <Ç¥. 1>¿¡´Â ÃøÁ¤¿¡ ¿µÇâÀ» ÁÖÁö ¾Ê´Â ³óµµ¸¦ Ç¥±âÇÏ¿´´Ù.

<Ç¥. 1> ´Ü¹éÁú Á¤·® »ùÇÃÀÇ °øÁ¸¹°Áú Çã¿ë³óµµ
´Ü¹éÁú Á¾·ù¿¡ µû¸¥ ¿µÇâ
BCA assay´Â ´Ü¹éÁú Á¾·ù¿¡ µû¸¥ ÃøÁ¤ °ªÀÇ º¯µ¿ÀÌ Àû´Ù´Â Ư¡ÀÌ ÀÖ´Ù.

<±×¸². 2>´Â ÀϹÝÀûÀ¸·Î »ç¿ëµÇ´Â Ç¥Áع°Áú BSA¿Í BGG¸¦ ´Ü°èÈñ¼® ÈÄ ÃøÁ¤ÇÏ¿© Ç¥ÁØ°î¼±À» ÀÛ¼ºÇÏ¿´´Ù. <Ç¥. 2>´Â 15Á¾·ùÀÇ ´Ü¹éÁúÀÇ ¹ß»ö·üÀ» BSAÀÇ ¹ß»ö·ü°ú ºñ±³ÇÏ¿´´Ù.

<±×¸². 2> 0~2,000 ¥ìg/§¢ ³óµµ ¹üÀ§ÀÇ BSA¿Í BGGÀÇ Ç¥ÁØ °î¼±

<Ç¥. 2> Ç¥ÁØ ÇÁ·ÎÅäÄÝÀÇ BSA¿¡ ´ëÇÑ °¢Á¾ ´Ü¹éÁúÀÇ ¹ß»ö·ü

*Ratio: (°¢Á¾ ´Ü¹éÁúÀÇ Èí±¤µµ Æò±Õ°ª) / (BSAÀÇ Èí±¤µµ Æò±Õ°ª)

Keyword :

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