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> TaKaRa BCA Protein Assay Kit
BCA ´Ü¹éÁú Á¤·® Kit
TaKaRa BCA Protein Assay Kit
Á¦Á¶»ç
Á¦Ç°ÄÚµå
Á¦Ç°¸í
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°¡°Ý
(ºÎ°¡¼¼º°µµ)
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»ç¿ëÀڸŴº¾ó
Takara
T9300A
TaKaRa BCA Protein Assay Kit
500ȸ
145,600¿ø
182,000¿ø
09.02 ~ 10.31
Product index
Overview
´Ü¹éÁú ¹ßÇö½Ã½ºÅÛ - »ó..
[°³¿ä] Â÷¼¼´ë ´Ü¹éÁú ..
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
[¼±Åð¡À̵å] Protein E..
His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦
[¼±Åð¡À̵å] His-tag ..
Capturem¢â His-Tagged ..
TALON (Cobalt) resin ..
TALON Magnetic Beads
TALON (Cobalt) Superfl..
xTractor Buffer Kit
His60 (Nickel) Superfl..
His60 Ni Magnetic Bead..
HRV 3C Protease
Poly Histidine tag Ç×..
TALON (Cobalt) CellThr..
TALONSpin Column
´Ü¹éÁú ÃßÃâ ¡¤ Á¤Á¦°ü·Ã
Calpastatin
Cryonase¢â Cold-active..
Glutathione Excellose ..
IDA Excellose (polyhis..
MBP Excellose (MBP fus..
MiniExcellose
ProteoGuard EDTA-Free ..
Streptavidin-based Enr..
Ç×ü Á¤Á¦
Ç×ü Á¤Á¦ Resin
Capturem¢â IP £¦ Co-IP..
°ïÃæ ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö (Bac..
[Overview] Insect Expr..
BacPAK¢â Baculovirus G..
BacPAK¢â p35 ELISA Kit..
BacPAK Baculovirus ¹ß..
Baculovirus control vi..
Baculovirus DNA packag..
Baculovirus Titer Kit
flashBAC¢â Baculovirus..
FoldHelper Helper viru..
ProEasy Baculovirus Ve..
ProFectin
ProFold Baculovirus Ve..
qPCR Titer ÃøÁ¤ Kit
Transfer Vectors
Æ÷À¯·ù ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö
[Overview] Mammalian E..
CHOgro¢ç Expression Sy..
CHOgro¢ç High Yield Ex..
Corystein¢â£¨Purothion..
Human Cell-Free Protei..
pHEK293 Ultra Expressi..
´ëÀå±Õ ´Ü¹éÁú ¹ßÇö
[Overview] Bacterial E..
´ëÀå±Õ ´Ü¹éÁú ¹ßÇö £¦ ..
Anti-ProS2
Anti-TF(Trigger Factor..
Chaperone Competent Ce..
Chaperone Plasmid Set
HAT ´Ü¹éÁú ¹ßÇö£¦ Á¤Á¦..
pCold¢â GST DNA
pCold¢â ProS2 DNA
pCold¢â TF DNA
pCold¢â Vector ½Ã¸®Áî
Single Protein Product..
TaKaRa Competent Cell ..
Brevibacillus£¯Bacillus ¹ß..
B. subtilis Secretory ..
´Ù¾çÇÑ tag ´Ü¹éÁú °ü·Ã
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
Excellose Spin Kit His..
FLAG tag ´Ü¹éÁú »ý»ê
GST-tag À¶ÇÕ ´Ü¹éÁú Á¤..
´ç£¯ÀλêÈ ´Ü¹éÁú °ü·Ã
Glycoprotein ³óÃà¿ë Re..
Magnetic bead - Phosph..
Phosphoprotein enrichm..
´Ü¹éÁú ¿°»ö ½Ã¾à
TaKaRa CBB Protein Saf..
SYPRO Protein Gel Stai..
SYPRO Ruby Protein Sta..
´Ü¹éÁú °ËÃâ, Á¤·®, Çؼ®
HRP Substrate ½Ã¸®Áî
HRP-Juice
ProSieve¢â EX Running ..
TaKaRa BCA Protein Ass..
TaKaRa Bradford Protei..
Tris-Glycine Powder
Western BLoT Blocking/..
Western BLoT Immuno Bo..
Western BLoT Immuno Bo..
Western BLoT Rapid Det..
Protease (¾Æ¹Ì³ë»ê ¼¿ ÇØ..
Arginylendopeptidase (..
Pfu Aminopeptidase I
Pfu Methionine Aminope..
Pfu N-acetyl Deblockin..
Pfu Protease S
Pfu Pyroglutamate Amin..
Bicinchoninic acid (BCA) ½Ã¾àÀ» ÀÌ¿ëÇÑ ´Ü¹éÁú Á¤·® kit
°£´ÜÇÑ Á¶ÀÛÀ¸·Î ´Ü½Ã°£¿¡ ´Ü¹éÁú Á¤·® °¡´É
´Ü¹éÁú Á¾·ù¿¡ ¿µÇâÀ» ¹ÞÁö ¾Ê¾Æ 0.02~2 mg/§¢ÀÇ ³ÐÀº ¹üÀ§¿¡¼ ´Ü¹éÁú Á¤·® °¡´É
°è¸é È°¼ºÁ¦¿¡ ÀÇÇÑ ¿µÇâÀ» ¹ÞÁö ¾ÊÀ½
Á¦Ç° ¼³¸í
TaKaRa BCA Protein Assay KitÀº ¸Å¿ì ³ôÀº °¨µµÀÇ ¹ß»ö ¹ÝÀÀ(colormetric)À» ÀÌ¿ëÇÏ¿© ´Ü¹éÁúÀÇ ¾çÀ» Á¤·®Çϸç, °è¸éÈ°¼ºÁ¦·Î °¡¿ëÈµÈ ´Ü¹éÁúÀÇ Á¤·®µµ °¡´ÉÇÏ´Ù.
BCA ´Ü¹éÁú Á¤·®ÀÇ ¿ø¸®´Â 2´Ü°è·Î ÀÌ·ç¾îÁø´Ù. ù ¹ø° ´Ü°è´Â ¾ËÄ®¸®¼º(¾à ¿°±â¼º)¿ë¾×³»ÀÇ Á¤·®ÇÏ·Á´Â ´Ü¹éÁúÀÇ ÆéŸÀÌµå °áÇÕ¿¡ ÀÇÇØ Cu
+2
ÀÌ Cu
+1
À¸·Î ȯ¿øµÈ´Ù(biuret reacton). ÀÌ ¶§, ȯ¿øµÈ Cu
+1
ÀÇ ¾çÀº ¿ë¾×¿¡ Æ÷ÇÔµÈ ´Ü¹éÁúÀÇ ¾ç¿¡ ºñ·ÊÇÑ´Ù. µÎ ¹ø° ´Ü°è¿¡¼ 2ºÐÀÚÀÇ Bicinchoninic acid (BCA)°¡ ȯ¿øµÈ Cu
+1
¿Í ¹èÀ§ °áÇÕÇÏ¿© 562nm¿¡¼ °ÇÑ Èí±¤µµ¸¦ ³ªÅ¸³»´Â ÆĶû-º¸¶ó»öÀÇ ÈÇÕ¹°À» Çü¼ºÇÑ´Ù. ÀÌ°ÍÀ» ºÐ±¤ ±¤µµ°è(spectrophotometer)¸¦ »ç¿ëÇÏ¿© ÃøÁ¤ ¹× Á¤·®ÇÑ´Ù.
BCA¿¡ ÀÇÇÑ ´Ü¹éÁú Á¤·®Àº ´Ü¹éÁúÀÇ Á¾·ù¿¡ µû¸¥ Â÷ÀÌ°¡ Àû¾î 0.02~2 mg/§¢ÀÇ ³ÐÀº ¹üÀ§¿¡¼ Á¤·®ÀÌ °¡´ÉÇϸç, °è¸éÈ°¼ºÁ¦¿¡ ÀÇÇÑ ¿µÇâÀ» ¹ÞÁö ¾Ê´Â´Ù. ÇÏÁö¸¸, ȯ¿øÁ¦ ¹× ų·¹ÀÌÆ®°è ¹°ÁúÀº ¹ÝÀÀ¼º¿¡ ¿µÇâÀ» Áֱ⠶§¹®¿¡, ÇØ´ç ¹°ÁúÀÌ Æ÷ÇÔµÈ ¿ë¾×ÀÇ Á¤·®¿¡´Â ÀûÇÕÇÏÁö ¾Ê´Ù(°øÁ¸¹°ÁúÀÇ ¿µÇâÀº <Ç¥. 1> Âü°í).
´Ü¹éÁúÀÇ Á¤·®Àº º» Á¦Ç°¿¡ Æ÷ÇÔµÈ BSA Ç¥Áع°ÁúÀ» »ç¿ëÇÏ¿© °Ë·® °î¼±(standard curve)À» ÀÛ¼º ÈÄ »ùÇÃÀÇ ³óµµ¸¦ °áÁ¤ÇÑ´Ù.
±×¸² 1. Standard Curve for BSA from 0 - 2,000 ¥ìg/§¢
³»¿ë
1 §¢ ¹ÝÀÀ ½Ã 500ȸ, microtiter plate¸¦ ÀÌ¿ëÇÑ 200 §¡ ¹ÝÀÀ ½Ã 2,500ȸÀÇ ÃøÁ¤À» ÇÒ ¼ö ÀÖ´Ù.
1. BCA Reagent A
250 §¢¡¿2
2. BCA Reagent B
20 §¢
3. BSA Standard Solution (2 mg/ml)
*
1 §¢¡¿10
*
: BSA Standard SolutionÀº ¾ÈÁ¤È¸¦ À§ÇØ 0.9% NaCl, 0.05% NaN
3
¸¦ Æ÷ÇÔÇÑ´Ù.
º¸Á¸
BCA reagent A, BCA reagent B : ½Ç¿Â
BSA Standard solution : 4¡É
*º» Á¦Ç°Àº »ó¿Â¿¡¼ ¹è¼ÛµË´Ï´Ù. Á¦Ç° ¼ö·É ÈÄ BSA Standard SolutionÀº 4¡É¿¡ º¸°üÇϼ¼¿ä.
Á¦Ç° ÀÌ¿Ü¿¡ ÇÊ¿äÇÑ °Í
Spectrophotometer and compatible 1 §¢ cuvette
Microplate reader and compatible microplate
Benchtop centrifuge
Microtubes (2 §¢ or 1.5 §¢)
Water bath (37¡É)
Incubator (60¡É) (for the low-concentration measurement protocol)
´Ü¹éÁú Á¤·® »ùÇÿ¡ °øÁ¸ ¹°Áú¿¡ ÀÇÇÑ ¿µÇâ
BCA ¹æ¹ýÀº ºñ±³Àû °è¸éÈ°¼ºÁ¦³ª Buffer¿¡ ´ëÇÑ ¿µÇâÀ» ¹ÞÁö ¾ÊÁö¸¸, °í³óµµÀÇ Å³·¹ÀÌÆ®°è ¹× ȯ¿øÁ¦ ¹°ÁúÀº ÃøÁ¤¿¡ ¿µÇâÀ» ÁØ´Ù. <Ç¥. 1>¿¡´Â ÃøÁ¤¿¡ ¿µÇâÀ» ÁÖÁö ¾Ê´Â ³óµµ¸¦ Ç¥±âÇÏ¿´´Ù.
<Ç¥. 1> ´Ü¹éÁú Á¤·® »ùÇÃÀÇ °øÁ¸¹°Áú Çã¿ë³óµµ
´Ü¹éÁú Á¾·ù¿¡ µû¸¥ ¿µÇâ
BCA assay´Â ´Ü¹éÁú Á¾·ù¿¡ µû¸¥ ÃøÁ¤ °ªÀÇ º¯µ¿ÀÌ Àû´Ù´Â Ư¡ÀÌ ÀÖ´Ù.
<±×¸². 2>´Â ÀϹÝÀûÀ¸·Î »ç¿ëµÇ´Â Ç¥Áع°Áú BSA¿Í BGG¸¦ ´Ü°èÈñ¼® ÈÄ ÃøÁ¤ÇÏ¿© Ç¥ÁØ°î¼±À» ÀÛ¼ºÇÏ¿´´Ù. <Ç¥. 2>´Â 15Á¾·ùÀÇ ´Ü¹éÁúÀÇ ¹ß»ö·üÀ» BSAÀÇ ¹ß»ö·ü°ú ºñ±³ÇÏ¿´´Ù.
<±×¸². 2> 0~2,000 ¥ìg/§¢ ³óµµ ¹üÀ§ÀÇ BSA¿Í BGGÀÇ Ç¥ÁØ °î¼±
<Ç¥. 2> Ç¥ÁØ ÇÁ·ÎÅäÄÝÀÇ BSA¿¡ ´ëÇÑ °¢Á¾ ´Ü¹éÁúÀÇ ¹ß»ö·ü
*Ratio: (°¢Á¾ ´Ü¹éÁúÀÇ Èí±¤µµ Æò±Õ°ª) / (BSAÀÇ Èí±¤µµ Æò±Õ°ª)
Keyword :
´Ü¹éÁú °ËÃâ, Á¤·®, Çؼ®
Western blotting HRP °ËÃâ ÈÇÐ ¹ß±¤ ±âÁú
HRP Substrate ½Ã¸®Áî
HRP-Juice
ÃÖÀûÀÇ buffer·Î ´Ü¹éÁú Àü±â¿µµ¿À» ºü¸£°Ô
ProSieve¢â EX Running Buffers
BCA ´Ü¹éÁú Á¤·® Kit
TaKaRa BCA Protein Assay Kit
Bradford ¹æ½ÄÀÇ ´Ü¹éÁú Á¤·® Kit
TaKaRa Bradford Protein Assay Kit
Tris-Glycine Powder
Western BLoT Blocking/Stripping Buffer
Western blot, ELISAÀÇ Ç×ü ¹ÝÀÀ ÃËÁøÁ¦
Western BLoT Immuno Booster
Western blot, ELISAÀÇ Ç×ü ¹ÝÀÀ ÃËÁøÁ¦ (1 Li..
Western BLoT Immuno Booster PF
HRPÇ¥½Ä 2Â÷ Ç×ü ´ë½Å¿¡
Western BLoT Rapid Detect v2.0