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°ïÃæ ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö (Baculovirus)
> BacPAK¢â p35 ELISA Kit (Single Wash)
°£ÆíÇØÁø baculovirus ¿ª°¡ ÃøÁ¤ Å°Æ®
BacPAK¢â p35 ELISA Kit (Single Wash)
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Clontech
631477
BacPAK¢â p35 ELISA Kit (Single Wash)
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BaculovirusÀÇ titer¸¦ ELISA·Î °£ÆíÇÏ°Ô ÃøÁ¤
°¨¿°À¸·ÎºÎÅÍ 20½Ã°£ À̳»¿¡ ¿ª°¡ È®ÀÎ °¡´É, ¾à 2½Ã°£ÀÇ hands on time
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BacPAK p35 ELISA Kit (Single Wash)´Â baculovirus¿¡¼ ¹ßÇöÇÏ´Â p35ÀÇ ³óµµ (ng/§¢)¸¦ ELISA¹ýÀ¸·Î Á¤·®ÇÏ´Â Á¦Ç°À¸·Î, ÀÌ¹Ì ¿ª°¡¸¦ ¾Ë°í ÀÖ´Â reference virus stockÀÇ p35 °ª°ú ºñ±³/ȯ»ê
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À» ÅëÇØ ¾Ë°íÀÚ ÇÏ´Â baculovirus »ùÇÃÀÇ functional titer¸¦ °áÁ¤ÇÒ ¼ö ÀÖ´Ù.
p35´Â ¹ÙÀÌ·¯½ºÀÇ ¼¼Æ÷ °¨¿°¿¡ ÇʼöÀûÀÎ Ãʱ⠹ßÇö ´Ü¹éÁú·Î °¨¿° ½Ã ¼¼Æ÷ÀÇ apoptosis¸¦ ÀúÇØÇÑ´Ù. °¨¿° 4~12½Ã°£ ÈÄ ¼¼Æ÷Áú ±âÁú¿¡¼ °ËÃâµÇ¹Ç·Î, ¼¼Æ÷ÀÇ ÇüÅ º¯È³ª ¹ÙÀÌ·¯½º Ãâ¾Æ°¡ ÀϾ±â Àü¿¡ ¹ÙÀÌ·¯½º ´Ü¹éÁúÀÇ ¹ßÇöÁ¤µµ¸¦ Á¤È®ÇÏ°Ô ÃøÁ¤ÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°Àº ¾à 2½Ã°£ÀÇ hands on timeÀ¸·Î 20½Ã°£ À̳»¿¡ baculovirus ¿ª°¡¸¦ ÃøÁ¤Çϸç, 10
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»ùÇÃÀÇ ¿ª°¡¸¦ ÃøÁ¤Çϱâ Àü¿¡, ¹Ì¸® ¿ª°¡¸¦ ¾Ë°í ÀÖ´Â reference virus¸¦ Å×½ºÆ®ÇÏ¿© p35 °ªÀ» ¾òÀ¸¸é, ¿ª°¡ È®ÀÎÀÌ ÇÊ¿äÇß´ø »ùÇÃÀÇ pfu/§¢ (IFU/§¢) ´ÜÀ§ ¿ª°¡·Îµµ °è»ê(ȯ»ê)ÇÒ ¼ö ÀÖ´Ù.
- ½±°Ô ȯ»êÇÏ´Â ¿Â¶óÀÎ tool ¹Ù·Î°¡±â:
Baculovirus p35-to-IFU calculator (TB USA ÆäÀÌÁö·Î À̵¿ÇÕ´Ï´Ù.)
±×¸² 1. BacPAK¢â p35 ELISA Kit (Single Wash) workflow
A. ¹ÙÀÌ·¯½º »ùÇà serial dilution
B. Èñ¼®ÇÑ ¹ÙÀÌ·¯½º¸¦ °ïÃæ ¼¼Æ÷¿¡ °¨¿°½ÃŲ ÈÄ 27¡É, 16~20½Ã°£ incubation
C. ÷ºÎµÈ Lysis Buffer·Î °¨¿° ¼¼Æ÷¸¦ ¿ëÇØÇÏ°í ELISA·Î »ùÇÃÀÇ ¿ª°¡ (p35°ª)¸¦ ÃøÁ¤ (ng/§¢)
±×¸² 2. BacPAK¢â p35 ELISA Kit (Single Wash) ¿ø¸®
ELISA plate¿¡ cell lysate¿Í HRP-anti-p35 detection antibody¸¦ Ãß°¡Çϸé, »ùÇà ³»ÀÇ p35´Â Ç÷¹ÀÌÆ®¿¡ °íÁ¤ÈµÇ¾î ÀÖ´Â capture antiboody¿Í °áÇÕÇÏ°í, HRP-anti-p35 detection antibody¿Í °áÇÕÇÏ¿© Ç¥ÁöµÈ´Ù. Washing Buffer·Î ¼¼Ã´ ÈÄ, TMB Substrate·Î ¹ß»ö½ÃŲ´Ù. Stop SolutionÀ¸·Î ¹ÝÀÀÀ» Á¤Áö½ÃŲ ÈÄ, Èí±¤µµ (450 nm)¸¦ ÃøÁ¤ÇÏ¿© p35 ³óµµ (ng/§¢)¸¦ Á¤·®ÇÑ´Ù.
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Anti-p35 Coated Plate (96 well£º8 well¡¿12 strips)
p35 Control
Lysis Buffer
p35 Buffer
Anti-p35 (HRP Conjugate)
Washing Buffer (20¡¿)
TMB Substrate
Stop Solution
º¸Á¸ 4¡É
Washing Buffer (20¡¿)¸¦ ³ÃÀå º¸°üÇϸé ħÀüÀÌ ¹ß»ýÇÒ °¡´É¼ºÀÌ ÀÖ´Ù.
- ħÀüÀÌ ³ªÅ¸³ °æ¿ì 37¡É¿¡¼ ¿ëÇØÇÏ¿© »ç¿ë
°ü·ÃÁ¦Ç°
- Baculovirus ½Ã½ºÅÛ ¼Â¾÷À» À§ÇÑ all-in-one kit:
BacPAK¢â Baculovirus Expression System
- Insect cell line:
IPLB-Sf21 Insect Cells
- Insect cell Àü¿ë transfection ½Ã¾à:
Trans
IT
¢ç
-Insect Transfection Reagent
- ´Ù¾çÇÑ ¿ª°¡ÃøÁ¤ kit
qPCR·Î ºü¸£°Ô genome copy ÃøÁ¤:
BacPAK¢â qPCR Titration Kit
¸é¿ªÇÐÀû ºÐ¼®¹ýÀ¸·Î ±î´Ù·Î¿î plaque ºÐ¼® ºÒÇÊ¿ä:
BacPAK¢â Baculovirus Rapid Titer Kit
- ÇÕ¸®ÀûÀÎ °¡°Ý, °¡¼ººñ ³ôÀº qPCR ±â±â
CronoSTAR¢â 96 Real-Time PCR System(4ch)
Keyword :
°ïÃæ ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö (Baculovirus)
[Overview] Insect Expression System
10ºÐ ¿Ï·á - baculovirus ½Å¼Ó¿ª°¡ ÃøÁ¤
BacPAK¢â Baculovirus GoStix¢â Plus
°£ÆíÇØÁø baculovirus ¿ª°¡ ÃøÁ¤ Å°Æ®
BacPAK¢â p35 ELISA Kit (Single Wash)
BacPAK Baculovirus ¹ßÇö ½Ã½ºÅÛ
Positive / negative control ¿ë baculovirus
Baculovirus control virus / transfer vector
Baculovirus DNA & transfer vector set
Baculovirus DNA package kit
¸é¿ªÇÐÀû ÃøÁ¤¹ýÀ¸·Î 48 ½Ã°£À¸·Î Baculovirus ..
Baculovirus Titer Kit
°ïÃæ, Æ÷À¯·ù ¼¼Æ÷¿ë ÀçÁ¶ÇÕ ´Ü¹éÁú °í¹ßÇö ½Ã..
flashBAC¢â Baculovirus Expression System
ºÐÀÚ ChaperoneÀ» ¹ßÇöÇÏ´Â Recombinant Baculo..
FoldHelper Helper virus
Plaque Assay ¼øÈ°¡ ºÒÇÊ¿äÇÑ È¿À²ÀûÀÎ Recomb..
ProEasy Baculovirus Vectors
Sf9, Sf21 cell¿¡ ÃÖÀûÈµÈ Transfection reage..
ProFectin
Chaperone Æ÷ÇÔ - ´Ü¹éÁú °¡¿ëÈ È¿À² Çâ»ó
ProFold Baculovirus Vectors
Baculovirus ¿ª°¡£¨Genomic DNA copy ¼ö£©¸¦ ..
qPCR Titer ÃøÁ¤ Kit
¸ñÀû¿¡ µû¶ó ¼±ÅÃÇÏ¿© Baculovirus Á¦ÀÛ
Transfer Vectors