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ºü¸£°Ô ¼Ò¿ë·®ÀÇ his-tagged protein Á¤Á¦

TALON Magnetic Beads

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
635636
TALON Magnetic Beads
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 ml¡¿2
371,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33125
Clontech
635637
TALON Magnetic Beads
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 ml¡¿6
1,029,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
635638
TALON Magnetic Beads Buffer Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 Kit
538,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33125
Clontech
631964
Magnetic Stand
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 Set
114,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

  • His-tagged proteinÀÇ ½Å¼ÓÇÏ°í °£ÆíÇÑ Á¤Á¦
  • TALON¢çÀÇ ³ôÀº ģȭ¼º°ú ƯÀ̼ºÀ¸·Î ¸ñÀû ´Ü¹éÁúÀÇ ¼øµµ Çâ»ó
  • ÀûÀº ºÎÇÇ (50-200 §¡)¿¡¼­ elution
Á¦Ç° ¼³¸í
TALON¢ç Magnetic Bead´Â his-tagged protein¿¡ ³ôÀº ģȭ¼º°ú ƯÀ̼ºÀ» °¡Áø TALON¢ç resin°ú °£ÆíÈ÷ ºÐ¸®ÇÒ ¼ö ÀÖ´Â magnetic beadsÀÇ ÀåÁ¡À» °áÇÕÇÑ Á¦Ç°ÀÌ´Ù. Magnetic beads´Â cobalt À̿°ú °áÇյǾî ÀÖ¾î his-tagged protein¿¡ º¸´Ù ´õ ³ôÀº ƯÀ̼ºÀ» °¡Áö¸ç, cobalt ÀÌ¿ÂÀº TALON¢çÀÇ tetradentate metal chelator¿¡ ÀÇÇØ beads¿Í °áÇյǾî ÀÖ¾î, Á¤Á¦ Áß¿¡ ±Ý¼ÓÀÌ¿ÂÀÌ Å»¶ôÇÏ´Â °ÍÀ» ¸·´Â´Ù.

°íµµÀÇ Æ¯ÀÌÀû °áÇÕ & ÃßÃâ
¾Æ·¡ÀÇ ±×¸²°ú °°ÀÌ TALON¢ç Magnetic Bead´Â ³ôÀº ƯÀ̼ºÀ¸·Î ÀÛÀº ºÐÀÚ·®¿¡¼­ Å« ºÐÀÚ·®±îÁö ´Ù¾çÇÑ Å©±âÀÇ his-tagged protein°ú °áÇÕÇϸç, Á¤Á¦µÈ ´Ü¹éÁúÀº ¼Ò¿ë·® (50-200§¡)À¸·Î elutionµÇ¹Ç·Î ³óÃàµÈ ÇüÅ·ΠÁ¤Á¦ÇÒ ¼ö ÀÖ´Ù (~3 §·/§¢). TALON¢ç Magnetic Bead´Â 5% ÇöŹ¾×À¸·Î Á¦°øµÇ¸ç, §¢ ´ç 750 §¶ÀÇ ´Ü¹éÁú°ú °áÇÕÇÒ ¼ö ÀÖ´Ù.


±×¸². TALON¢ç Magnetic Beads¸¦ ÀÌ¿ëÇÑ his-tagged protein Á¤Á¦
E. coli BL21¿¡¼­ ¹ßÇöµÈ ´Ü¹éÁúÀ» xTractor¢â Buffer (Code 635623)·Î ÃßÃâÇÏ¿© TALON¢ç Magnetic Beads·Î Á¤Á¦ÇÑ °á°ú °í¼øµµ·Î ´Ü¹éÁúÀÌ Á¤Á¦ÇÒ ¼ö ÀÖ¾ú´Ù (Lane 1: Starting E. coli extract. Lane 2: Nonadsorbed material. Lane 3: Eluted protein. Lane M: Molecular weight markers.).
TALON¢ç resinÀÇ Æ¯Â¡
TALON resin
TALON¢ç resinÀº cobalt¸¦ ÀÌ¿ëÇØ his-tagged proteinÀ» Á¤Á¦ÇÏ´Â immobilized metal affinity chromatography (IMAC) resinÀÌ´Ù. Cobalt´Â nickelº¸´Ù his-tagged protein¿¡ ´ëÇØ ³ôÀº ƯÀ̼ºÀ» ³ªÅ¸³»¹Ç·Î his-tagged proteinÀ» °í¼øµµ·Î Á¤Á¦ÇÒ ¼ö ÀÖ´Ù.
Cobalt¸¦ Æ÷ÇÔÇÑ °áÇÕ ºÎÀ§
TALON¢ç Metal Affinity ResinÀº °áÇÕ ºÎÀ§¿¡ cobalt ÀÌ¿ÂÀ» Æ÷ÇÔÇÏ°í ÀÖ¾î, his-tagged protein¿¡ ´ëÇØ ¸Å¿ì ³ôÀº ƯÀ̼ºÀ» °¡Áö°í ÀÖ´Ù. Cobalt ÀÌ¿ÂÀ» Æ÷ÇÔÇÑ TALON¢ç °áÇÕºÎÀ§´Â histidineÀÇ À§Ä¡¿¡ ¸Å¿ì ±î´Ù·Î¿ö histidineÀÌ °ø°£ÀûÀ¸·Î ¿¬¼ÓÇØ Àְųª, °¡±îÀÌ À§Ä¡ÇÑ (6x HN tag, HAT tag µî) ´Ü¹éÁú¸¸ resin¿¡ °áÇÕÇÒ ¼ö ÀÖ´Ù. ¹Ý¸é nickel ±â¹ÝÀÇ resin (Ni-NTA resin µî)Àº ÀÌ·¯ÇÑ Æ¯ÀÌÀûÀÎ ¼­¿­ÀÌ ¾Æ´Ñ histidineÀÌ ºÒ±ÔÄ¢ÇÏ°Ô ¹èÄ¡µÈ ¼­¿­¿¡µµ °áÇÕÇϹǷÎ, »ó´ëÀûÀ¸·Î cobalt ±â¹ÝÀÇ TALON¢ç resinÀÌ nickel¿¡ ºñÇØ his-tagged proteinÀ» ƯÀ̼º ³ô°Ô Á¤Á¦ÇÒ ¼ö ÀÖ´Ù.
±Ý¼ÓÀÌ¿Â Å»¶ôÀÌ ÀûÀº ±ÕÀÏÇÑ matrix ±¸Á¶
Cobalt resinÀº nickel resin¿¡ ºñÇØ ´õ ±ÕÀÏÇÑ ±¸Á¶¸¦ °¡Áø´Ù. TALON¢ç resinÀº 3Â÷ Æ÷Äϱ¸Á¶¸¦ Çü¼ºÇÏ¸ç °¢°¢ 3°³ÀÇ carboxyl ±×·ì°ú ÇϳªÀÇ Áú¼Ò¿øÀÚ°¡ cobalt ¾çÀ̿°ú °áÇÕÇÑ ±¸Á¶·Î, ÀÌ·¯ÇÑ ±¸Á¶´Â cobalt ÀÌ¿ÂÀÌ 2°³ÀÇ ÀÎÁ¢ÇÑ histidine Àܱâ¿Í °áÇյǵµ·Ï ÇÑ´Ù. µû¶ó¼­ cobalt ÀÌ¿ÂÀÌ ¸Å¿ì ´Ü´ÜÇÏ°Ô °áÇյǾî ÀÖ¾î resin¿¡¼­ ´©ÃâµÇÁö ¾Ê´Â´Ù. ¹Ý¸é nickel resinÀº nickel ÀÌ¿ÂÀÌ TALON¢ç ligand¿Í À¯»çÇÑ 3Â÷ Æ÷Äϱ¸Á¶³ª Æò¸é±¸Á¶, Áï 2°³ÀÇ ´Ù¸¥ ¹èÀ§±¸Á¶¸¦ Çü¼ºÇÒ ¼ö ÀÖ¾î TALON¢ç¿¡ ºñÇØ ±¸Á¶°¡ ´ú ±ÕÀÏÇÏ´Ù. Æò¸é ±¸Á¶¿¡¼­ nickel ÀÌ¿ÂÀº 2°³ÀÇ carboxyl ±×·ì°ú ÇϳªÀÇ Áú¼Ò¿øÀÚ¿¡ °áÇÕÇÏ°í ÀÖ¾î TALON¢ç¿¡ ºñÇØ °áÇÕÀÌ ¾àÇÏ°í nickel ÀÌ¿ÂÀ» °­ÇÏ°Ô À¯ÁöÇÒ ¼ö ¾ø¾î resin¿¡¼­ÀÇ ´©ÃâÀÌ ÀϾ´Ù. ±ÕÀÏÇÑ matrix¸¦ Çü¼ºÇÏ´Â TALON¢ç Metal Affinity ResinÀº ¿©·¯ Á¤Á¦ Á¶°Ç ÇÏ¿¡¼­ ³ôÀº °áÇÕ´É·ÂÀ» °¡Áö¹Ç·Î È®½ÇÇÏ°Ô his-tagged proteinÀ» Á¤Á¦ÇÒ ¼ö ÀÖ´Ù.


±×¸². TALON¢ç Magnetic Beads & Magnetic Stand
º¸Á¸ 4¡É
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
°ü·ÃÁ¦Ç°
- Protein Á¤Á¦¸¦ À§ÇÑ Àü¿ë lysis buffer : xTractor¢â Buffer Kit
- His-tagged protein °í¹ßÇöÀ» À§ÇÑ cold shock vector system : pCold¢â vector
- Acetic acid ¾ø´Â °í°¨µµ ´Ü¹éÁú ¿°»ö½Ã¾à : TaKaRa CBB Protein Safe Stain
- ¹ü¿ëÀûÀÎ °ËÃâºÎÅÍ ÃÊ°í°¨µµ °ËÃâ±îÁö ´Ù¾çÇÑ Á¦Ç° ¶óÀξ÷ : HRP Substrate ½Ã¸®Áî
- ½Ç¿Â¿¡¼­ 30ºÐ ¿Ï·á : Western BLoT Stripping Buffer (Code T7135A)

Keyword :

His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦
>
Spin columnŸÀÔÀ¸·Î ºü¸£°í °£ÆíÇÑ His-tag ´Ü..
Capturem¢â His-Tagged Purification
>
His-tagged protein °í¼øµµ Á¤Á¦
TALON (Cobalt) resin & column
>
ºü¸£°Ô ¼Ò¿ë·®ÀÇ his-tagged protein Á¤Á¦
TALON Magnetic Beads
>
FPLC purification - His-tagged protein
TALON (Cobalt) Superflow resin & cartridge
>
´ëÀå±ÕÀ¸·ÎºÎÅÍ His-tag À¶ÇÕ ´Ü¹éÁú ÃßÃâ¿¡,xT..
xTractor Buffer Kit
>
°í¼öÀ² Ni resin & FPLC purification
His60 (Nickel) Superflow resin & cartridge
>
High-throughput & microscale
His60 Ni Magnetic Beads
>
4¡É¿¡¼­ È°¼ºÀ» ¶ç´Â HN-tag¸¦ °®°í ÀÖ´Â ´Ü¹é..
HRV 3C Protease
>
Cell lysate¿¡¼­ His-tagged protein Á÷Á¢ Á¤Á¦
TALON (Cobalt) CellThru resin
>
Prepacked spin column
TALONSpin Column