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´Ü¹éÁú ¹ßÇö ¡¤ Á¤Á¦
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°ïÃæ ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö (Baculovirus)
> BacPAK Baculovirus ¹ßÇö ½Ã½ºÅÛ
BacPAK Baculovirus ¹ßÇö ½Ã½ºÅÛ
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Clontech
631402
BacPAK Baculovirus Expression System
1 Set
(Package)
2,208,000¿ø
,
,
,
,
Clontech
631401
BacPAK6 DNA (Bsu36 I digest)
5 ȸ
1,159,000¿ø
Clontech
631411
IPLB-Sf21 Insect Cells
1 vial
1,095,000¿ø
Clontech
631721
X-GLUC
100 mg
791,000¿ø
Product index
Overview
´Ü¹éÁú ¹ßÇö½Ã½ºÅÛ - »ó..
[°³¿ä] Â÷¼¼´ë ´Ü¹éÁú ..
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
[¼±Åð¡À̵å] Protein E..
His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦
[¼±Åð¡À̵å] His-tag ..
Capturem¢â His-Tagged ..
TALON (Cobalt) resin ..
TALON Magnetic Beads
TALON (Cobalt) Superfl..
xTractor Buffer Kit
His60 (Nickel) Superfl..
His60 Ni Magnetic Bead..
HRV 3C Protease
Poly Histidine tag Ç×..
TALON (Cobalt) CellThr..
TALONSpin Column
´Ü¹éÁú ÃßÃâ ¡¤ Á¤Á¦°ü·Ã
Calpastatin
Cryonase¢â Cold-active..
Glutathione Excellose ..
IDA Excellose (polyhis..
MBP Excellose (MBP fus..
MiniExcellose
ProteoGuard EDTA-Free ..
Streptavidin-based Enr..
Ç×ü Á¤Á¦
Ç×ü Á¤Á¦ Resin
Capturem¢â IP £¦ Co-IP..
°ïÃæ ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö (Bac..
[Overview] Insect Expr..
BacPAK¢â Baculovirus G..
BacPAK¢â p35 ELISA Kit..
BacPAK Baculovirus ¹ß..
Baculovirus control vi..
Baculovirus DNA packag..
Baculovirus Titer Kit
flashBAC¢â Baculovirus..
FoldHelper Helper viru..
ProEasy Baculovirus Ve..
ProFectin
ProFold Baculovirus Ve..
qPCR Titer ÃøÁ¤ Kit
Transfer Vectors
Æ÷À¯·ù ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö
[Overview] Mammalian E..
CHOgro¢ç Expression Sy..
CHOgro¢ç High Yield Ex..
Corystein¢â£¨Purothion..
Human Cell-Free Protei..
pHEK293 Ultra Expressi..
´ëÀå±Õ ´Ü¹éÁú ¹ßÇö
[Overview] Bacterial E..
´ëÀå±Õ ´Ü¹éÁú ¹ßÇö £¦ ..
Anti-ProS2
Anti-TF(Trigger Factor..
Chaperone Competent Ce..
Chaperone Plasmid Set
HAT ´Ü¹éÁú ¹ßÇö£¦ Á¤Á¦..
pCold¢â GST DNA
pCold¢â ProS2 DNA
pCold¢â TF DNA
pCold¢â Vector ½Ã¸®Áî
Single Protein Product..
TaKaRa Competent Cell ..
Brevibacillus£¯Bacillus ¹ß..
B. subtilis Secretory ..
´Ù¾çÇÑ tag ´Ü¹éÁú °ü·Ã
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
Excellose Spin Kit His..
FLAG tag ´Ü¹éÁú »ý»ê
GST-tag À¶ÇÕ ´Ü¹éÁú Á¤..
´ç£¯ÀλêÈ ´Ü¹éÁú °ü·Ã
Glycoprotein ³óÃà¿ë Re..
Magnetic bead - Phosph..
Phosphoprotein enrichm..
´Ü¹éÁú ¿°»ö ½Ã¾à
TaKaRa CBB Protein Saf..
SYPRO Protein Gel Stai..
SYPRO Ruby Protein Sta..
´Ü¹éÁú °ËÃâ, Á¤·®, Çؼ®
HRP Substrate ½Ã¸®Áî
HRP-Juice
ProSieve¢â EX Running ..
TaKaRa BCA Protein Ass..
TaKaRa Bradford Protei..
Tris-Glycine Powder
Western BLoT Blocking/..
Western BLoT Immuno Bo..
Western BLoT Immuno Bo..
Western BLoT Rapid Det..
Protease (¾Æ¹Ì³ë»ê ¼¿ ÇØ..
Arginylendopeptidase (..
Pfu Aminopeptidase I
Pfu Methionine Aminope..
Pfu N-acetyl Deblockin..
Pfu Protease S
Pfu Pyroglutamate Amin..
BacPAK Baculovirus Expression SystemÀº °ïÃæ ¼÷ÁÖ ¼¼Æ÷¿¡¼ ÀçÁ¶ÇÕ ´Ü¹éÁúÀ» °í¹ßÇö (¹è¾ç¾× L ´ç 1-500 mgÀÇ ´Ü¹éÁú)½ÃŲ´Ù (1, 2). ClontechÀÇ BacPAK SystemÀº 3 °¡Áö Áß¿äÇÑ ÀåÁ¡À» °®°í ÀÖ´Ù.
ÀçÁ¶ÇÕ ´Ü¹éÁúÀÇ ³ôÀº ȸ¼öÀ² : °ïÃæ ¼÷ÁÖ ¼¼Æ÷´Â ¸¹Àº ¾çÀÇ ¸ñÀû ´Ü¹éÁúÀ» »ý»êÇÑ´Ù.
»ýü ´Ü¹éÁú°úÀÇ À¯»ç¼ºÀÌ ³ôÀ½ : °ïÃæ ¼÷ÁÖ ¼¼Æ÷°¡ mammalian ¼¼Æ÷¿Í À¯»çÇÑ ¹ø¿ª ÈÄ (post-translational)ÀÇ °úÁ¤À» Á¦°øÇϱ⠶§¹®¿¡ ¹ßÇöµÈ ÀçÁ¶ÇÕ ´Ü¹éÁúÀº ÀϹÝÀûÀÎ »ýü ´Ü¹éÁú°ú ±¸Á¶ÀûÀ¸·Î À¯»çÇÒ »Ó¸¸ ¾Æ´Ï¶ó, »ý¹°ÇÐÀû È°¼º°ú ¸é¿ªÇÐÀû ¹ÝÀÀ¼º ¶ÇÇÑ À¯»çÇÏ´Ù.
³ôÀº ÀçÁ¶ÇÕ È¿À² : ÇüÁú ÀüȯµÈ ¼¼Æ÷¿¡ ÀÇÇØ »ý¼ºµÇ´Â ¹ÙÀÌ·¯½ºÀÇ 90% ÀÌ»óÀº ¸ñÀû ´Ü¹éÁúÀ» »ý»êÇÑ´Ù. BacPAK6 Viral DNA´Â ¹ÙÀÌ·¯½º¿Í Àü´ÞµÈ vector »çÀÌÀÇ ÀçÁ¶ÇÕÀ» À¯µµÇÏ¿© ³ôÀº ÀçÁ¶ÇÕ È¿À²À» ³ªÅ¸³»°Ô µÈ´Ù.
Figure 1. Protein production from recombinant viruses generated using the BacPAK Baculovirus Expression System.
Recombinant viruses were obtained by cotransfection of transfer vectors with BacPAK6 Viral DNA (Bsu36 I digest), followed by amplification in Sf21 cells. The SDS PAGE analysis of cellular lysates was performed 48 hr after infection of the Sf21 cultures. Lane 1: uninfected Sf21 cells. Lane 2: Sf21 cells infected with wild-type AcMNPV virus. Lane 3: Sf21 cells infected with nonrecombinant BacPAK6 virus. Lane 4: Sf21 cells infected with BacPAK8-GUS recombinant virus. Lane 5: purified CAT protein. Lane 6: Sf21 cells infected with BacPAK9-CAT recombinant virus. Lane M: molecular weight marker.
BacPAK ¹æ¹ý
¸ñÀû À¯ÀüÀÚ´Â shuttle vector·Î »ðÀԵǰí linearized BacPAK6 Viral DNA¿Í ÇÔ²² °ïÃæ ¼÷ÁÖ ¼¼Æ÷·Î cotransfection µÈ´Ù. BacPAK6 DNA¿¡´Â baculovirus genomeÀÇ Çʼö ¿ä¼Ò°¡ ¾øÁö¸¸ DNA°¡ vector¿Í ÀçÁ¶ÇÕÇϸé Çʼö ¿ä¼Ò°¡ º¹±¸µÇ°í ¸ñÀû À¯ÀüÀÚ°¡ baculovirus genomeÀ¸·Î ³¢¾îµé¾î°£´Ù. ÀçÁ¶ÇÕ ÈÄ, ¸î °³ÀÇ ¹ÙÀÌ·¯½º plaque¸¦ ¼±ÅÃ, Á¤Á¦ÇÏ¿© ÀçÁ¶ÇÕ Ç¥ÇöÇüÀ» È®ÀÎÇÑ´Ù. ±×·± ÈÄ »õ·Î ºÐ¸®µÈ ÀçÁ¶ÇÕ ¹ÙÀÌ·¯½º¸¦ ÁõÆøÇÏ¿© °ïÃæ ¼¼Æ÷ ¹è¾ç¾×¿¡ °¨¿°½ÃÅ°¸é ¸¹Àº ¾çÀÇ ¸ñÀû ´Ü¹éÁúÀ» ¸¸µé¾î³¾ ¼ö ÀÖ´Ù.
BacPAK SystemÀº BacPAK6 Viral DNA transfer vector, ÀçÁ¶ÇÕ ´Ü¹éÁú »ý»ê¿¡ ÇÊ¿äÇÑ °ïÃæ ¼÷ÁÖ¼¼Æ÷, °íÈ¿À² ÇüÁúÀüȯÀ» À§ÇÑ Bacfectin Transfection ½Ã¾à, sequencing°ú PCRÀ» À§ÇÑ primer¸¦ Æ÷ÇÔÇÏ°í ÀÖ´Ù. Baculovirus titer¸¦ ºü¸£°Ô ÃøÁ¤Çϱâ À§Çؼ BacPAK Rapid Titer Kit (Cat. No. 631406)³ª BacPAK qPCR Titration Kit (Cat. No. 631414)¸¦ ÃßõÇÑ´Ù. BacPAK6 Viral DNA (Cat. No. 631401)¿Í IPLB-Sf21 Insect Cells (Cat. No. 631411)´Â º°µµ ±¸¸Åµµ °¡´ÉÇÏ´Ù.
Figure 2. BacPAK6 Viral DNA map.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
Cell Line Ãë±Þ ÁÖÀÇ»çÇ× (Ŭ¸¯)
References
1. Kitts, P. A. & Possee, R. D. (1993) Biotechniques 15(5):810-817.
2. Kitts, P. A. et al. (1990) Nucleic Acids Res. 18:5667-5672.
Keyword : ´Ü¹éÁú¹ßÇö, Baculovirus, Baculo, º£Å§·Î¹ÙÀÌ·¯½º, ¹èŧ·Î¹ÙÀÌ·¯½º, °ïÃæ¼¼Æ÷, °ïÃæ´Ü¹éÁú, °ïÃæ, Insect, ´Ü¹éÁú»ý»ê, ´Ü¹éÁú ´ë·®»ý»ê
°ïÃæ ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö (Baculovirus)
[Overview] Insect Expression System
10ºÐ ¿Ï·á - baculovirus ½Å¼Ó¿ª°¡ ÃøÁ¤
BacPAK¢â Baculovirus GoStix¢â Plus
°£ÆíÇØÁø baculovirus ¿ª°¡ ÃøÁ¤ Å°Æ®
BacPAK¢â p35 ELISA Kit (Single Wash)
BacPAK Baculovirus ¹ßÇö ½Ã½ºÅÛ
Positive / negative control ¿ë baculovirus
Baculovirus control virus / transfer vector
Baculovirus DNA & transfer vector set
Baculovirus DNA package kit
¸é¿ªÇÐÀû ÃøÁ¤¹ýÀ¸·Î 48 ½Ã°£À¸·Î Baculovirus ..
Baculovirus Titer Kit
°ïÃæ, Æ÷À¯·ù ¼¼Æ÷¿ë ÀçÁ¶ÇÕ ´Ü¹éÁú °í¹ßÇö ½Ã..
flashBAC¢â Baculovirus Expression System
ºÐÀÚ ChaperoneÀ» ¹ßÇöÇÏ´Â Recombinant Baculo..
FoldHelper Helper virus
Plaque Assay ¼øÈ°¡ ºÒÇÊ¿äÇÑ È¿À²ÀûÀÎ Recomb..
ProEasy Baculovirus Vectors
Sf9, Sf21 cell¿¡ ÃÖÀûÈµÈ Transfection reage..
ProFectin
Chaperone Æ÷ÇÔ - ´Ü¹éÁú °¡¿ëÈ È¿À² Çâ»ó
ProFold Baculovirus Vectors
Baculovirus ¿ª°¡£¨Genomic DNA copy ¼ö£©¸¦ ..
qPCR Titer ÃøÁ¤ Kit
¸ñÀû¿¡ µû¶ó ¼±ÅÃÇÏ¿© Baculovirus Á¦ÀÛ
Transfer Vectors