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BacPAK Baculovirus ¹ßÇö ½Ã½ºÅÛ

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
631402
BacPAK Baculovirus Expression System
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 Set
(Package)
2,208,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x33274, x33275, x32501, x32502, x99314
Clontech
631401
BacPAK6 DNA (Bsu36 I digest)
°ü·ÃÇмú ±¸¸ÅÇϱâ
5 ȸ
1,159,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
631411
IPLB-Sf21 Insect Cells
°ü·ÃÇмú ±¸¸ÅÇϱâ
1 vial
1,095,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
631721
X-GLUC
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 mg
791,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â



BacPAK Baculovirus Expression SystemÀº °ïÃæ ¼÷ÁÖ ¼¼Æ÷¿¡¼­ ÀçÁ¶ÇÕ ´Ü¹éÁúÀ» °í¹ßÇö (¹è¾ç¾× L ´ç 1-500 mgÀÇ ´Ü¹éÁú)½ÃŲ´Ù (1, 2). ClontechÀÇ BacPAK SystemÀº 3 °¡Áö Áß¿äÇÑ ÀåÁ¡À» °®°í ÀÖ´Ù.
  • ÀçÁ¶ÇÕ ´Ü¹éÁúÀÇ ³ôÀº ȸ¼öÀ² : °ïÃæ ¼÷ÁÖ ¼¼Æ÷´Â ¸¹Àº ¾çÀÇ ¸ñÀû ´Ü¹éÁúÀ» »ý»êÇÑ´Ù.
  • »ýü ´Ü¹éÁú°úÀÇ À¯»ç¼ºÀÌ ³ôÀ½ : °ïÃæ ¼÷ÁÖ ¼¼Æ÷°¡ mammalian ¼¼Æ÷¿Í À¯»çÇÑ ¹ø¿ª ÈÄ (post-translational)ÀÇ °úÁ¤À» Á¦°øÇϱ⠶§¹®¿¡ ¹ßÇöµÈ ÀçÁ¶ÇÕ ´Ü¹éÁúÀº ÀϹÝÀûÀÎ »ýü ´Ü¹éÁú°ú ±¸Á¶ÀûÀ¸·Î À¯»çÇÒ »Ó¸¸ ¾Æ´Ï¶ó, »ý¹°ÇÐÀû È°¼º°ú ¸é¿ªÇÐÀû ¹ÝÀÀ¼º ¶ÇÇÑ À¯»çÇÏ´Ù.
  • ³ôÀº ÀçÁ¶ÇÕ È¿À² : ÇüÁú ÀüȯµÈ ¼¼Æ÷¿¡ ÀÇÇØ »ý¼ºµÇ´Â ¹ÙÀÌ·¯½ºÀÇ 90% ÀÌ»óÀº ¸ñÀû ´Ü¹éÁúÀ» »ý»êÇÑ´Ù. BacPAK6 Viral DNA´Â ¹ÙÀÌ·¯½º¿Í Àü´ÞµÈ vector »çÀÌÀÇ ÀçÁ¶ÇÕÀ» À¯µµÇÏ¿© ³ôÀº ÀçÁ¶ÇÕ È¿À²À» ³ªÅ¸³»°Ô µÈ´Ù.

Figure 1. Protein production from recombinant viruses generated using the BacPAK Baculovirus Expression System. Recombinant viruses were obtained by cotransfection of transfer vectors with BacPAK6 Viral DNA (Bsu36 I digest), followed by amplification in Sf21 cells. The SDS PAGE analysis of cellular lysates was performed 48 hr after infection of the Sf21 cultures. Lane 1: uninfected Sf21 cells. Lane 2: Sf21 cells infected with wild-type AcMNPV virus. Lane 3: Sf21 cells infected with nonrecombinant BacPAK6 virus. Lane 4: Sf21 cells infected with BacPAK8-GUS recombinant virus. Lane 5: purified CAT protein. Lane 6: Sf21 cells infected with BacPAK9-CAT recombinant virus. Lane M: molecular weight marker.
BacPAK ¹æ¹ý
¸ñÀû À¯ÀüÀÚ´Â shuttle vector·Î »ðÀԵǰí linearized BacPAK6 Viral DNA¿Í ÇÔ²² °ïÃæ ¼÷ÁÖ ¼¼Æ÷·Î cotransfection µÈ´Ù. BacPAK6 DNA¿¡´Â baculovirus genomeÀÇ Çʼö ¿ä¼Ò°¡ ¾øÁö¸¸ DNA°¡ vector¿Í ÀçÁ¶ÇÕÇϸé Çʼö ¿ä¼Ò°¡ º¹±¸µÇ°í ¸ñÀû À¯ÀüÀÚ°¡ baculovirus genomeÀ¸·Î ³¢¾îµé¾î°£´Ù. ÀçÁ¶ÇÕ ÈÄ, ¸î °³ÀÇ ¹ÙÀÌ·¯½º plaque¸¦ ¼±ÅÃ, Á¤Á¦ÇÏ¿© ÀçÁ¶ÇÕ Ç¥ÇöÇüÀ» È®ÀÎÇÑ´Ù. ±×·± ÈÄ »õ·Î ºÐ¸®µÈ ÀçÁ¶ÇÕ ¹ÙÀÌ·¯½º¸¦ ÁõÆøÇÏ¿© °ïÃæ ¼¼Æ÷ ¹è¾ç¾×¿¡ °¨¿°½ÃÅ°¸é ¸¹Àº ¾çÀÇ ¸ñÀû ´Ü¹éÁúÀ» ¸¸µé¾î³¾ ¼ö ÀÖ´Ù.

BacPAK SystemÀº BacPAK6 Viral DNA transfer vector, ÀçÁ¶ÇÕ ´Ü¹éÁú »ý»ê¿¡ ÇÊ¿äÇÑ °ïÃæ ¼÷ÁÖ¼¼Æ÷, °íÈ¿À² ÇüÁúÀüȯÀ» À§ÇÑ Bacfectin Transfection ½Ã¾à, sequencing°ú PCRÀ» À§ÇÑ primer¸¦ Æ÷ÇÔÇÏ°í ÀÖ´Ù. Baculovirus titer¸¦ ºü¸£°Ô ÃøÁ¤Çϱâ À§Çؼ­ BacPAK Rapid Titer Kit (Cat. No. 631406)³ª BacPAK qPCR Titration Kit (Cat. No. 631414)¸¦ ÃßõÇÑ´Ù. BacPAK6 Viral DNA (Cat. No. 631401)¿Í IPLB-Sf21 Insect Cells (Cat. No. 631411)´Â º°µµ ±¸¸Åµµ °¡´ÉÇÏ´Ù.


Figure 2. BacPAK6 Viral DNA map.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
Cell Line Ãë±Þ ÁÖÀÇ»çÇ× (Ŭ¸¯)
References
1. Kitts, P. A. & Possee, R. D. (1993) Biotechniques 15(5):810-817.
2. Kitts, P. A. et al. (1990) Nucleic Acids Res. 18:5667-5672.

Keyword : ´Ü¹éÁú¹ßÇö, Baculovirus, Baculo, º£Å§·Î¹ÙÀÌ·¯½º, ¹èŧ·Î¹ÙÀÌ·¯½º, °ïÃæ¼¼Æ÷, °ïÃæ´Ü¹éÁú, °ïÃæ, Insect, ´Ü¹éÁú»ý»ê, ´Ü¹éÁú ´ë·®»ý»ê

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