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´Ü¹éÁú ¹ßÇö ¡¤ Á¤Á¦
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His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦
> TALON (Cobalt) CellThru resin
Cell lysate¿¡¼ His-tagged protein Á÷Á¢ Á¤Á¦
TALON (Cobalt) CellThru resin
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Clontech
635509
TALON CellThru Resin
10 ml
292,000¿ø
,
Clontech
635510
TALON CellThru Resin
100 ml
2,014,000¿ø
Clontech
635513
CellThru 10-ml Disposable Columns
20 °³
286,000¿ø
,
Clontech
635651
HisTALON Buffer Set
20 ȸ
472,000¿ø
,
,
,
Product index
Overview
´Ü¹éÁú ¹ßÇö½Ã½ºÅÛ - »ó..
[°³¿ä] Â÷¼¼´ë ´Ü¹éÁú ..
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
[¼±Åð¡À̵å] Protein E..
His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦
[¼±Åð¡À̵å] His-tag ..
Capturem¢â His-Tagged ..
TALON (Cobalt) resin ..
TALON Magnetic Beads
TALON (Cobalt) Superfl..
xTractor Buffer Kit
His60 (Nickel) Superfl..
His60 Ni Magnetic Bead..
HRV 3C Protease
Poly Histidine tag Ç×..
TALON (Cobalt) CellThr..
TALONSpin Column
´Ü¹éÁú ÃßÃâ ¡¤ Á¤Á¦°ü·Ã
Calpastatin
Cryonase¢â Cold-active..
Glutathione Excellose ..
IDA Excellose (polyhis..
MBP Excellose (MBP fus..
MiniExcellose
ProteoGuard EDTA-Free ..
Streptavidin-based Enr..
Ç×ü Á¤Á¦
Ç×ü Á¤Á¦ Resin
Capturem¢â IP £¦ Co-IP..
°ïÃæ ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö (Bac..
[Overview] Insect Expr..
BacPAK¢â Baculovirus G..
BacPAK¢â p35 ELISA Kit..
BacPAK Baculovirus ¹ß..
Baculovirus control vi..
Baculovirus DNA packag..
Baculovirus Titer Kit
flashBAC¢â Baculovirus..
FoldHelper Helper viru..
ProEasy Baculovirus Ve..
ProFectin
ProFold Baculovirus Ve..
qPCR Titer ÃøÁ¤ Kit
Transfer Vectors
Æ÷À¯·ù ¼¼Æ÷ ´Ü¹éÁú ¹ßÇö
[Overview] Mammalian E..
CHOgro¢ç Expression Sy..
CHOgro¢ç High Yield Ex..
Corystein¢â£¨Purothion..
Human Cell-Free Protei..
pHEK293 Ultra Expressi..
´ëÀå±Õ ´Ü¹éÁú ¹ßÇö
[Overview] Bacterial E..
´ëÀå±Õ ´Ü¹éÁú ¹ßÇö £¦ ..
Anti-ProS2
Anti-TF(Trigger Factor..
Chaperone Competent Ce..
Chaperone Plasmid Set
HAT ´Ü¹éÁú ¹ßÇö£¦ Á¤Á¦..
pCold¢â GST DNA
pCold¢â ProS2 DNA
pCold¢â TF DNA
pCold¢â Vector ½Ã¸®Áî
Single Protein Product..
TaKaRa Competent Cell ..
Brevibacillus£¯Bacillus ¹ß..
B. subtilis Secretory ..
´Ù¾çÇÑ tag ´Ü¹éÁú °ü·Ã
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
Excellose Spin Kit His..
FLAG tag ´Ü¹éÁú »ý»ê
GST-tag À¶ÇÕ ´Ü¹éÁú Á¤..
´ç£¯ÀλêÈ ´Ü¹éÁú °ü·Ã
Glycoprotein ³óÃà¿ë Re..
Magnetic bead - Phosph..
Phosphoprotein enrichm..
´Ü¹éÁú ¿°»ö ½Ã¾à
TaKaRa CBB Protein Saf..
SYPRO Protein Gel Stai..
SYPRO Ruby Protein Sta..
´Ü¹éÁú °ËÃâ, Á¤·®, Çؼ®
HRP Substrate ½Ã¸®Áî
HRP-Juice
ProSieve¢â EX Running ..
TaKaRa BCA Protein Ass..
TaKaRa Bradford Protei..
Tris-Glycine Powder
Western BLoT Blocking/..
Western BLoT Immuno Bo..
Western BLoT Immuno Bo..
Western BLoT Rapid Det..
Protease (¾Æ¹Ì³ë»ê ¼¿ ÇØ..
Arginylendopeptidase (..
Pfu Aminopeptidase I
Pfu Methionine Aminope..
Pfu N-acetyl Deblockin..
Pfu Protease S
Pfu Pyroglutamate Amin..
ºü¸£°í °£ÆíÇÑ ÇÁ·ÎÅäÄÝ: crude cell lysate¿¡¼ Á÷Á¢ Á¤Á¦
¸·´Ü¹éÁú ¶Ç´Â multiprotein complexÀÇ Á¤Á¦¿¡ ÃÖÀû
Cobalt ÀÌ¿ÂÀ» ÀÌ¿ëÇÑ IMAC resin : Nickel resin º¸´Ù °í¼øµµ·Î his-tagged protein Á¤Á¦
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TALON
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CellThru ResinÀº cell lysate, ÃÊÀ½ÆÄ Æļ⠻ùÇÃ, fermenter¿¡¼ ȸ¼öÇÑ ¼¼Æ÷ µî¿¡¼ his-tagged proteinÀ» Á¤Á¦ÇÒ ¼ö ÀÖ´Â IMAC resinÀÌ´Ù. ±âÁ¸ÀÇ TALON
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Metal Affinity Resin°ú °°ÀÌ µ¶ÀÚÀûÀÎ ¸®°£µå¸¦ °¡Áö°í ÀÖÁö¸¸ ´õ Å« beads (300-500§)¸¦ »ç¿ëÇÏ¿© ¿ø½ÉºÐ¸® ¾øÀ̵µ ¼¼Æ÷ ÀÜÇع°ÀÌ resinÀ» Åë°úÇÒ ¼ö ÀÖ´Ù. ½Å¼ÓÇÑ 1 step °úÁ¤À¸·Î crude lysate¿¡¼ his-tagged proteinÀ» Á÷Á¢ Á¤Á¦ÇϹǷÎ, ±âÁ¸ ¹æ¹ý º¸´Ù ´Ü¹éÁú ºÐÇØ °¡´É¼ºÀ» ³·Ãß°í ³ôÀº ¼öÀ²·Î ´Ü¹éÁúÀ» Á¤Á¦ÇÒ ¼ö ÀÖ´Ù.
ÀϹÝÀûÀÎ resinÀ» »ç¿ëÇϸé cell lysate¸¦ column¿¡ Àû¿ëÇϱâ Àü¿¡ ¿ø½ÉºÐ¸® µîÀ» ÅëÇØ cell debris¸¦ Á¦°ÅÇÏ´Â °úÁ¤ÀÌ ÇÊ¿äÇѵ¥, ÀÌ ¶§ Á¤Á¦ÇÏ°íÀÚ ÇÏ´Â ¸· °áÇÕ ´Ü¹éÁú ¶Ç´Â ´Ü¹éÁú º¹ÇÕü°¡ ¼¼Æ÷ ÀÜ¿©¹°°ú ÇÔ²² Á¦°ÅµÇ´Â °æ¿ì°¡ ÀÖ´Ù. ¹Ý¸é, TALON
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CellThru ResinÀ» ÀÌ¿ëÇϸé cell lysateÀÇ ¿ø½ÉºÐ¸® °úÁ¤ ¾øÀÌ ±×´ë·Î resin¿¡ Àû¿ëÇÒ ¼ö ÀÖÀ¸¹Ç·Î, »ùÇà ¾È¿¡ Á¸ÀçÇÏ´Â ¸· °áÇÕ ´Ü¹éÁú ¶Ç´Â ´Ü¹éÁú º¹ÇÕü¸¦ ¼º°øÀûÀ¸·Î Á¤Á¦ÇÒ ¼ö ÀÖ´Ù.
Å©±â°¡ Å« agarose beads·Î ±¸¼ºµÈ º» resinÀº ±¸¸ÛÀÌ ¸·È÷´Â °ÍÀ» ¸·±â À§ÇØ Áö¸§ÀÌ Å« ÀÏ¹Ý chromatography column¿¡ ÃæÁøÇؼ »ç¿ëÇϰųª, ¼¼Æ÷ ÀÜ¿©¹°À» ½±°Ô Åë°ú½Ãų ¼ö ÀÖµµ·Ï 90~130§ÀÇ ÇÊÅ͸¦ ÀåÂøÇÏ¿© ÆǸŠÁßÀÎ CellThru-10§¢ Disposable Column°ú ÇÔ²² »ç¿ëÇÏ¸é µÈ´Ù.
TALON
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resinÀÇ Æ¯Â¡
TALON resin
TALON
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resinÀº cobalt¸¦ ÀÌ¿ëÇØ his-tagged proteinÀ» Á¤Á¦ÇÏ´Â immobilized metal affinity chromatography (IMAC) resinÀÌ´Ù. Cobalt´Â nickelº¸´Ù his-tagged protein¿¡ ´ëÇØ ³ôÀº ƯÀ̼ºÀ» ³ªÅ¸³»¹Ç·Î his-tagged proteinÀ» °í¼øµµ·Î Á¤Á¦ÇÒ ¼ö ÀÖ´Ù.
Cobalt¸¦ Æ÷ÇÔÇÑ °áÇÕ ºÎÀ§
TALON
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Metal Affinity ResinÀº °áÇÕ ºÎÀ§¿¡ cobalt ÀÌ¿ÂÀ» Æ÷ÇÔÇÏ°í ÀÖ¾î, his-tagged protein¿¡ ´ëÇØ ¸Å¿ì ³ôÀº ƯÀ̼ºÀ» °¡Áö°í ÀÖ´Ù. Cobalt ÀÌ¿ÂÀ» Æ÷ÇÔÇÑ TALON
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°áÇÕºÎÀ§´Â histidineÀÇ À§Ä¡¿¡ ¸Å¿ì ±î´Ù·Î¿ö histidineÀÌ °ø°£ÀûÀ¸·Î ¿¬¼ÓÇØ Àְųª, °¡±îÀÌ À§Ä¡ÇÑ (6x HN tag, HAT tag µî) ´Ü¹éÁú¸¸ resin¿¡ °áÇÕÇÒ ¼ö ÀÖ´Ù. ¹Ý¸é nickel ±â¹ÝÀÇ resin (Ni-NTA resin µî)Àº ÀÌ·¯ÇÑ Æ¯ÀÌÀûÀÎ ¼¿ÀÌ ¾Æ´Ñ histidineÀÌ ºÒ±ÔÄ¢ÇÏ°Ô ¹èÄ¡µÈ ¼¿¿¡µµ °áÇÕÇϹǷÎ, »ó´ëÀûÀ¸·Î cobalt ±â¹ÝÀÇ TALON
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resinÀÌ nickel¿¡ ºñÇØ his-tagged proteinÀ» ƯÀ̼º ³ô°Ô Á¤Á¦ÇÒ ¼ö ÀÖ´Ù.
±Ý¼ÓÀÌ¿Â Å»¶ôÀÌ ÀûÀº ±ÕÀÏÇÑ matrix ±¸Á¶
Cobalt resinÀº nickel resin¿¡ ºñÇØ ´õ ±ÕÀÏÇÑ ±¸Á¶¸¦ °¡Áø´Ù. TALON
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resinÀº 3Â÷ Æ÷Äϱ¸Á¶¸¦ Çü¼ºÇÏ¸ç °¢°¢ 3°³ÀÇ carboxyl ±×·ì°ú ÇϳªÀÇ Áú¼Ò¿øÀÚ°¡ cobalt ¾çÀ̿°ú °áÇÕÇÑ ±¸Á¶·Î, ÀÌ·¯ÇÑ ±¸Á¶´Â cobalt ÀÌ¿ÂÀÌ 2°³ÀÇ ÀÎÁ¢ÇÑ histidine Àܱâ¿Í °áÇյǵµ·Ï ÇÑ´Ù. µû¶ó¼ cobalt ÀÌ¿ÂÀÌ ¸Å¿ì ´Ü´ÜÇÏ°Ô °áÇյǾî ÀÖ¾î resin¿¡¼ ´©ÃâµÇÁö ¾Ê´Â´Ù. ¹Ý¸é nickel resinÀº nickel ÀÌ¿ÂÀÌ TALON
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ligand¿Í À¯»çÇÑ 3Â÷ Æ÷Äϱ¸Á¶³ª Æò¸é±¸Á¶, Áï 2°³ÀÇ ´Ù¸¥ ¹èÀ§±¸Á¶¸¦ Çü¼ºÇÒ ¼ö ÀÖ¾î TALON
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¿¡ ºñÇØ ±¸Á¶°¡ ´ú ±ÕÀÏÇÏ´Ù. Æò¸é ±¸Á¶¿¡¼ nickel ÀÌ¿ÂÀº 2°³ÀÇ carboxyl ±×·ì°ú ÇϳªÀÇ Áú¼Ò¿øÀÚ¿¡ °áÇÕÇÏ°í ÀÖ¾î TALON
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¿¡ ºñÇØ °áÇÕÀÌ ¾àÇÏ°í nickel ÀÌ¿ÂÀ» °ÇÏ°Ô À¯ÁöÇÒ ¼ö ¾ø¾î resin¿¡¼ÀÇ ´©ÃâÀÌ ÀϾÙ. ±ÕÀÏÇÑ matrix¸¦ Çü¼ºÇÏ´Â TALON
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Metal Affinity ResinÀº ¿©·¯ Á¤Á¦ Á¶°Ç ÇÏ¿¡¼ ³ôÀº °áÇÕ´É·ÂÀ» °¡Áö¹Ç·Î È®½ÇÇÏ°Ô his-tagged proteinÀ» Á¤Á¦ÇÒ ¼ö ÀÖ´Ù.
±×¸². TALON
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CellThru Resin
À» ÀÌ¿ëÇÑ ´Ü¹éÁú Á¤Á¦
E
.
coli
BL21 À» TALON
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wash buffer¿¡¼ sonication ÇÑ ÈÄ TALON
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CellThru columnÀ» ÀÌ¿ëÇÏ¿© 150 mM imidazole·Î Á¤Á¦ÇÏ¿´´Ù. M: molecular weight marker.
(Note: Membrane fraction¿¡ ³²¾ÆÀִ Ÿ°Ù ´Ü¹éÁúÀÇ ÀϺδ nonabsorbed material ±¸È¹¿¡¼ È®ÀεÊ)
±×¸². TALON
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CellThru Resin & CellThru 10-§¢ Disposable Columns
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TALON
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CellThru£º4¡É
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
°ü·ÃÁ¦Ç°
- Protein Á¤Á¦¸¦ À§ÇÑ Àü¿ë lysis buffer :
xTractor¢â Buffer Kit
- His-tagged protein °í¹ßÇöÀ» À§ÇÑ cold shock vector system :
pCold¢â vector
- Acetic acid ¾ø´Â °í°¨µµ ´Ü¹éÁú ¿°»ö½Ã¾à :
TaKaRa CBB Protein Safe Stain
- ¹ü¿ëÀûÀÎ °ËÃâºÎÅÍ ÃÊ°í°¨µµ °ËÃâ±îÁö ´Ù¾çÇÑ Á¦Ç° ¶óÀξ÷ :
HRP Substrate ½Ã¸®Áî
- ½Ç¿Â¿¡¼ 30ºÐ ¿Ï·á :
Western BLoT Stripping Buffer (Code T7135A)
Keyword :
His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦
[¼±Åð¡À̵å] His-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦
Spin columnŸÀÔÀ¸·Î ºü¸£°í °£ÆíÇÑ His-tag ´Ü..
Capturem¢â His-Tagged Purification
His-tagged protein °í¼øµµ Á¤Á¦
TALON (Cobalt) resin & column
ºü¸£°Ô ¼Ò¿ë·®ÀÇ his-tagged protein Á¤Á¦
TALON Magnetic Beads
FPLC purification - His-tagged protein
TALON (Cobalt) Superflow resin & cartridge
´ëÀå±ÕÀ¸·ÎºÎÅÍ His-tag À¶ÇÕ ´Ü¹éÁú ÃßÃâ¿¡,xT..
xTractor Buffer Kit
°í¼öÀ² Ni resin & FPLC purification
His60 (Nickel) Superflow resin & cartridge
High-throughput & microscale
His60 Ni Magnetic Beads
4¡É¿¡¼ È°¼ºÀ» ¶ç´Â HN-tag¸¦ °®°í ÀÖ´Â ´Ü¹é..
HRV 3C Protease
Poly Histidine tag Ç×ü
Cell lysate¿¡¼ His-tagged protein Á÷Á¢ Á¤Á¦
TALON (Cobalt) CellThru resin
Prepacked spin column
TALONSpin Column