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GST-tag À¶ÇÕ ´Ü¹éÁú Á¤Á¦ Resin

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
635607
Glutathione-Superflow Resin
°ü·ÃÇмú ±¸¸ÅÇϱâ
10 ml
483,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32678
Clontech
635608
Glutathione-Superflow Resin
°ü·ÃÇмú ±¸¸ÅÇϱâ
100 ml
3,792,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
Clontech
635619
GST Purification Kit
°ü·ÃÇмú ±¸¸ÅÇϱâ
5 ȸ
1,127,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â x32678

  • °í¼øµµÀÇ GST-tagged proteinÀ» one-stepÀ¸·Î Á¤Á¦
  • ³ôÀº °áÇÕ·Â (resin 1ml ´ç 10 mg ÀÌ»óÀÇ tagged protein)
  • Gravity flow¿Í FPLC Àû¿ëÀ» À§ÇÏ¿© ´Ù¾çÇÑ ÇüÅ·ΠÀÌ¿ë°¡´É
  • ¿ëÀÌÇÑ Àç»ý & ÇÕ¸®ÀûÀÎ °¡°Ý
Flexible Resin Format
Glutathione-Superflow¿Í Glutathione-Uniflow ResinÀº ³ôÀº ģȭ·Â°ú ƯÀ̼ºÀ¸·Î GST (glutathione-S-transferase) tag°ú °áÇÕÇÏ¿© GST-tagged proteinÀ» ºü¸£°í È¿À²ÀûÀ¸·Î Á¤Á¦Çϵµ·Ï ÇÑ´Ù. ÀÌ resinÀº glutathioneÀÌ °øÀ¯ °áÇյǾî ÀÖ´Â °¢°¢ 6%¿Í 4% cross-linked agarose¿¡ ±â¹ÝÀ» µÎ°í ÀÖ´Ù.

Glutathione-Superflow ResinÀº FPLC Àû¿ë¿¡ ÀûÇÕÇÏ´Ù. ÀÌ°ÍÀº ´õ ³ôÀº À¯¼Ó°ú 15 ml/cm2/min Á¤µµ·Î ³ôÀº À¯¼ÓÀ» °®´Â back pressureÀ» °ßµô ¼ö ÀÖ´Ù.

´õ Å« Æí¸®ÇÔÀ» À§ÇØ, GST Purification Kit´Â 5 ¹øÀÇ batch/gravity flow Á¤Á¦¸¦ À§ÇÑ ÃæºÐÇÑ stock buffer¿Í prepacked Glutathione-Superflow ColumnÀ» Á¦°øÇÑ´Ù. ÀÌ kit¸¦ »ç¿ëÇϸé column ´ç ÃÖ´ë 10 mgÀÇ GST-tagged proteinÀ» Á¤Á¦ÇÒ ¼ö ÀÖ´Ù.


Figure 1. GST-tagged protein purification from whole cell extract. Whole cell extracts containing GST-DHFR (Panel A) and GST alone (Panel B) were loaded, washed, and eluted from glutathione resin columns. The resulting purification fractions were analyzed by SDS-PAGE (upper panels) and Western blot (lower panels) with an anti-GST IgG. WCE = whole cell extract. FT = flowthrough. ELU = eluate.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.

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