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[Kit] In-Fusion® Cloning

제조사	제품코드	제품명	용량	가격 (부가세별도)	사용자매뉴얼
Clontech	639649	In-Fusion® HD Cloning Kit	50 회	1,409,000원	,
Clontech	639650	In-Fusion® HD Cloning Kit	100 회	2,439,000원	,
Clontech	639615	Cloning Enhancer	100 회	393,000원
Clontech	636763	Stellar™ Competent Cells	10 회 (10 x 100ul)	293,000원
Clontech	636767	Stellar™ Competent Cells (96 well plate)	96 회 (96 x 20ul) (Package)	822,000원

Product index

※ In-Fusion Cloning 생생 후기보기

*In-Fusion® HD Cloning Kit 는 In-Fusion® Snap Assembly Master Mix로 업그레이드 되었습니다.

PCR Cloning Kits―DNA Cloning―In Fusion HD Cloning System

In-Fusion Cloning System은 어떤 PCR 단편이나 복수의 DNA 단편도 선형화된 vector(linearized vector)에 15분(In-Fusion HD EcoDry는 30분)의 반응으로 directional cloning이 가능하다 (Figure 1). 또한 PCR 단편은 제한효소처리나 phosphorylation과 같은 추가적인 처리 단계가 필요하지 않다. In-Fusion 효소는 선형화된 vector 말단과 PCR로 증폭된 PCR 단편(insert)의 말단의 15bp homologue sequence를 인식하여 융합시킨다. 이 15 bp의 융합서열은 원하는 sequence를 증폭하고자 하는 PCR primer에 디자인하여 껴 넣으면 쉽게 만들 수 있다.

이 실험방법을 사용하면 아래와 같은 다양한 실험에 적용 가능하다.

Subcloning 없이도 복수의 DNA 단편을 원하는 vector에 Cloning
내부의 특정 부분만 교체하는 Modular expression vector 제작
불필요한 염기가 없는 Fusion protein 구축
DNA 단편의 제거나 교체
Insert의 point mutation이나 제한효소 사이트 첨가
유전자내 형광 단백질의 tag 교체
cDNA로 UTRs첨가

Figure 1. The In-Fusion cloning protocol.

Figure 2. The In-Fusion HD Cloning System has been optimized for cloning large fragments. Ten out of ten colonies contain the correct insert (100% efficiency) when cloning fragments as large as 15 kb (results confirmed by colony PCR screening).

Figure 3. The In-Fusion HD Cloning System has an improved capability for cloning multiple fragments in a single reaction. Using this system, cloning up to four 1-kb fragments simultaneously is as easy as cloning a single fragment. This saves weeks that would otherwise be spent screening clones and subcloning.

Citations

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Nature Biotechnology    |    2022 Apr 25    |    PubMed ID: 35468954    |    Read Article

Distinct modes of mitochondrial metabolism uncouple T helper cell differentiation and function
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Saturation Editing of Genomic Regions by Multiplex Homology-Directed Repair
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Endocrine Deficiency As a Function of Radiation Dose to the Hypothalamus and Pituitary in Pediatric and Young Adult Patients With Brain Tumors
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SPARC enables genetic manipulation of precise proportions of cells.
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SARS-CoV-2 Infects the Brain Choroid Plexus and Disrupts the Blood-CSF Barrier in Human Brain Organoids
Cell Stem Cell    |    2020 Oct 13    |    PubMed ID: 33113348    |    Read Article

Mutations in SARS-CoV-2 variants of concern link to increased spike cleavage and virus transmission
Cell Host & Microbe    |    2022 Jan 21    |    PubMed ID: 35150638    |    Read Article

Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activity
Molecular cell    |    2022 Jun 08    |    PubMed ID: 35219382    |    Read Article

The Functional Proximal Proteome of Oncogenic Ras Includes mTORC2
Molecular cell    |    2019 Jan 10    |    PubMed ID: 30639242    |    Read Article

Identification of a novel deFADding activity in human, yeast and bacterial 5′ to 3′ exoribonucleases
Nucleic Acids Research    |    2022 Aug 26    |    PubMed ID: 35904778    |    Read Article

Experimental demonstration of operon formation catalyzed by insertion sequence
Nucleic Acids Research    |    2022 Feb 22    |    PubMed ID: 35066585    |    Read Article

Grand scale genome manipulation via chromosome swapping in Escherichia coli programmed by three one megabase chromosomes
Nucleic Acids Research    |    2021 Sep 07    |    PubMed ID: 33907814    |    Read Article

Drop-off-reinitiation triggered by EF-G-driven mistranslocation and its alleviation by EF-P
Nucleic Acids Research    |    2022 Mar 21    |    PubMed ID: 35188576    |    Read Article

Rational design of an XNA ligase through docking of unbound nucleic acids to toroidal proteins
Nucleic Acids Research    |    2019 Jul 26    |    PubMed ID: 31334814    |    Read Article

An siRNA-guided ARGONAUTE protein directs RNA polymerase V to initiate DNA methylation
Nature Plants    |    2021 Nov 08    |    PubMed ID: 34750500    |    Read Article

NIMA-related kinase 9 regulates the phosphorylation of the essential myosin light chain in the heart
Nature Communications    |    2022 Oct 20    |    Read Article

Activation of stably silenced genes by recruitment of a synthetic de-methylating module
Nature Communications    |    2022 Sep 23    |    PubMed ID: 36151095    |    Read Article

Translational induction of ATF4 during integrated stress response requires noncanonical initiation factors eIF2D and DENR
Nature Communications    |    2020 Sep 16    |    PubMed ID: 32938929    |    Read Article

Age-dependent loss of adipose Rubicon promotes metabolic disorders via excess autophagy
Nature Communications    |    2020 Aug 18    |    PubMed ID: 32811819    |    Read Article

The surfaceome of multiple myeloma cells suggests potential immunotherapeutic strategies and protein markers of drug resistance
Nature Communications    |    2022 Jul 15    |    PubMed ID: 35840578    |    Read Article

Tracking cryptic SARS-CoV-2 lineages detected in NYC wastewater
Nature Communications    |    2022 Feb 03    |    PubMed ID: 35115523    |    Read Article

ICER is requisite for Th17 differentiation
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Therapeutic homology-independent targeted integration in retina and liver
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Robust and tunable signal processing in mammalian cells via engineered covalent modification cycles
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An endoribonuclease-based feedforward controller for decoupling resource-limited genetic modules in mammalian cells
Nature Communications    |    2020 Nov 10    |    Read Article

Defunctionalizing intracellular organelles such as mitochondria and peroxisomes with engineered phospholipase A/acyltransferases
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A Single Immunization with Spike-Functionalized Ferritin Vaccines Elicits Neutralizing Antibody Responses against SARS-CoV-2 in Mice
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Characterization of a vaccine-elicited human antibody with sequence homology to VRC01-class antibodies that binds the C1C2 gp120 domain
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Exonuclease VII repairs quinolone-induced damage by resolving DNA gyrase cleavage complexes
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A two-step mechanism governing PARP1-DNA retention by PARP inhibitors
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Keyword : infusion,in fusion,cloning,클로닝,복수단편,인퓨전,프로믹스,premix, mutagenesis, mutation,soeasy,so easy

In-Fusion Cloning

[Overview] In-Fusion® Cloning

[개요] So Easy Product

Online Tools for In-Fusion

최고의 Colony 형성능과 정확한 PCR cloning

[NEW] In-Fusion® Snap Assembly

실온 보관 Master mix

[NEW] 동결건조 In-Fusion® Snap Assembly

Premix화! 시간단축! 효율성 향상!

[Kit] In-Fusion® Cloning

In-Fusion® cloning을 바로 진행하기 위한 lin..

In-Fusion® Ready Vectors